Entry
Name
iron(III)-enterobactin esterase;
fes (gene name);
pfeE (gene name);
enterochelin hydrolase;
enterochelin esterase;
ferric enterobactin esterase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
iron(III)-enterobactin hydrolase
Reaction(IUBMB)
iron(III)-enterobactin + 3 H2O = iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine (overall reaction);
(1a) iron(III)-enterobactin + H2O = iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex;
(1b) iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + H2O = iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + N-(2,3-dihydroxybenzoyl)-L-serine;
(1c) iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + H2O = iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + N-(2,3-dihydroxybenzoyl)-L-serine
Substrate
iron(III)-enterobactin;
H2O [CPD:
C00001 ];
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex;
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex
Product
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex;
N-(2,3-dihydroxybenzoyl)-L-serine [CPD:
C04204 ];
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex;
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex
Comment
The enzyme, isolated from the bacterium Escherichia coli, allows the bacterium to grow in limited iron conditions. It can also act on enterobactin (with no complexed iron) and the aluminium(III) analogue of iron(III)-enterobactin. The trimer formed is further hydrolysed to form the dimer and the monomer.
History
EC 3.1.1.108 created 2019
Orthology
K07214 iron(III)-enterobactin esterase
K23722 iron(III)-enterobactin esterase
Genes
ECD : ECDH10B_0544(fes) ECDH10B_0652(fes)
ECOO : ECRM13514_0606(fes)
ECOH : ECRM13516_0550(fes)
EDJ : ECDH1ME8569_0554(fes)
ECOI : ECOPMV1_00602(fes_1)
REE : electrica_03715(fes)
RTG : NCTC13098_05443(fes_2)
KIE : NCTC12125_02382(fes)
AHN : NCTC12129_03611(fes)
MPHG : MPHASIOC01_003279(fes)
YMA : DA391_03015 DA391_07265
SMAR : SM39_1907 SM39_3042 SM39_3654 SM39_4668
SMAC : SMDB11_1731 SMDB11_3460 SMDB11_4409
SMW : SMWW4_v1c03110(fes) SMWW4_v1c24640 SMWW4_v1c41540
SMAF : D781_2010 D781_3215 D781_3453
SLQ : M495_17670 M495_21080
SERF : L085_02500 L085_07275 L085_16415
SERS : SERRSCBI_01415 SERRSCBI_11590 SERRSCBI_20210
SRZ : AXX16_1624 AXX16_1856 AXX16_3119
SERM : CLM71_10240 CLM71_16995 CLM71_18150
SQU : E4343_19830 E4343_23555
SFJ : SAMEA4384070_0301(fes_1) SAMEA4384070_1827(fes_2) SAMEA4384070_4186(besA)
SOF : NCTC11214_02950(besA) NCTC11214_03236(fes)
SSUR : ATE40_009005 ATE40_017490 ATE40_022300
SURI : J0X03_03130 J0X03_11620(fes) J0X03_22430(fes)
SRHZ : FO014_16065 FO014_17240 FO014_23405
SNEM : NLX84_01345(fes) NLX84_12460(fes) NLX84_21460
SNEV : OI978_04975(fes) OI978_10080
SGRI : SGBXF1_03505(fes) SGBXF1_04154(besA)
SSAR : SSARUM_000280(fes) SSARUM_002385(fes) SSARUM_003418(fes) SSARUM_004056
SERB : QEP77_01515 QEP77_21580(fes)
SERN : GUC32_01485(fes) GUC32_12110(fes) GUC32_20815
SERT : TAN611_2170(fes) TAN611_3417(fes) TAN611_3667
SERH : GF111_11360(fes) GF111_16460
PARL : PEC302110_29330(fes)
DIC : Dpoa569_0001091(fes)
PAO : Pat9b_0494 Pat9b_5347
PANS : FCN45_02445 FCN45_21755
KPIE : N5580_16155(fes) N5580_19690(fes)
PANE : P6287_02460(fes) P6287_22525(fes)
PANZ : NR302_02520(fes) NR302_23035(fes)
MINT : C7M51_02067(fes_1) C7M51_04039(fes_2)
TPTY : NCTC11468_00311(fes)
XFU : XFF4834R_chr28840 XFF4834R_chr35160
VAU : VANGNB10_cI0889(vabH)
VIB : VspSTUT11_46260(fes)
POR : APT59_14305 APT59_21420
PPSL : BJP27_05285 BJP27_15020
PPRC : PFLCHA0_c27290(besA)
PEZ : HWQ56_03585 HWQ56_12500(fes)
PBEN : V6W80_14380 V6W80_14825(fes) V6W80_23145
PSIV : LZ023_00595 LZ023_21270(fes)
BTRM : SAMEA390648702347(bfeB)
AXX : ERS451415_05705(besA)
CCOS : Pan44_38220(besA_2)
HBS : IPV69_15170 IPV69_15565
MRUB : DEO27_001720 DEO27_013405
» show all
Taxonomy
Reference
Authors
O'Brien IG, Cox GB, Gibson F
Title
Enterochelin hydrolysis and iron metabolism in Escherichia coli.
Journal
Sequence
Reference
Authors
Greenwood KT, Luke RK
Title
Enzymatic hydrolysis of enterochelin and its iron complex in Escherichia Coli K-12. Properties of enterochelin esterase.
Journal
Sequence
Reference
Authors
Pettis GS, McIntosh MA
Title
Molecular characterization of the Escherichia coli enterobactin cistron entF and coupled expression of entF and the fes gene.
Journal
Reference
Authors
Brickman TJ, McIntosh MA
Title
Overexpression and purification of ferric enterobactin esterase from Escherichia coli. Demonstration of enzymatic hydrolysis of enterobactin and its iron complex.
Journal
J Biol Chem 267:12350-5 (1992)
Sequence
Reference
Authors
Winkelmann G, Cansier A, Beck W, Jung G
Title
HPLC separation of enterobactin and linear 2,3-dihydroxybenzoylserine derivatives: a study on mutants of Escherichia coli defective in regulation (fur), esterase (fes) and transport (fepA).
Journal
Sequence
Reference
Authors
Perraud Q, Moynie L, Gasser V, Munier M, Godet J, Hoegy F, Mely Y, Mislin GLA, Naismith JH, Schalk IJ
Title
A Key Role for the Periplasmic PfeE Esterase in Iron Acquisition via the Siderophore Enterobactin in Pseudomonas aeruginosa.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.1.1.108