KEGG   ENZYME: 3.1.1.109
Entry
EC 3.1.1.109                Enzyme                                 
Name
iron(III)-salmochelin esterase;
iroD (gene name);
ferric-salmochelin esterase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Sysname
iron(III)-salmochelin complex hydrolase
Reaction(IUBMB)
(1) iron(III)-[diglucosyl-enterobactin] complex + H2O = iron(III)-[salmochelin S2] complex;
(2) iron(III)-[monoglucosyl-enterobactin] complex + H2O = iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex;
(3) iron(III)-[salmochelin S2] complex + H2O = iron(III)-[diglucosyl-(2,3-dihydroxybenzoylserine)2] complex + N-(2,3-dihydroxybenzoyl)-L-serine;
(4) iron(III)-[salmochelin S2] complex + H2O = iron(III)-[salmochelin S1] complex + salmochelin SX;
(5) iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex + H2O = iron(III)-[salmochelin S1] complex + N-(2,3-dihydroxybenzoyl)-L-serine;
(6) iron(III)-[diglucosyl-(2,3-dihydroxybenzoylserine)2] complex + H2O = iron(III)-[salmochelin SX] complex + salmochelin SX
Substrate
iron(III)-[diglucosyl-enterobactin] complex;
H2O [CPD:C00001];
iron(III)-[monoglucosyl-enterobactin] complex;
iron(III)-[salmochelin S2] complex;
iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex;
iron(III)-[diglucosyl-(2,3-dihydroxybenzoylserine)2] complex
Product
iron(III)-[salmochelin S2] complex;
iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex;
iron(III)-[diglucosyl-(2,3-dihydroxybenzoylserine)2] complex;
N-(2,3-dihydroxybenzoyl)-L-serine [CPD:C04204];
iron(III)-[salmochelin S1] complex;
salmochelin SX;
iron(III)-[salmochelin SX] complex
Comment
This bacterial enzyme is present in pathogenic Salmonella species, uropathogenic and avian pathogenic Escherichia coli strains, and certain Klebsiella strains. The enzyme acts on iron(III)-bound enterobactin and C-glucosylated derivatives known as salmochelins. Unlike EC 3.1.1.107, apo-salmochelin esterase (IroE), IroD prefers iron(III)-bound siderophores as substrates, and is assumed to act after the iron-siderophore complexes are imported into the cell. It catalyses several hydrolytic reactions, producing a mixture of iron(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] complex and salmochelin SX.
History
EC 3.1.1.109 created 2019
Orthology
K23723  iron(III)-salmochelin esterase
Genes
ESOO3O_09570
ESMO3M_15710
ECPECP_0304
ECVAPECO1_O1CoBM139(iroD)
ECIUTI89_C1120(iroD)
EIHECOK1_1109
ECZECS88_p0126(iroD)
ECCc1252(iroD)
ELOEC042_3201(mchS1)
ELNNRG857_30012(iroD)
EABECABU_c12090(iroD)
ELUUM146_12305
ELCi14_1146(iroD)
ELDi02_1146(iroD)
ECOIECOPMV1_01080(fes_2)
STYSTY2892(iroD)
STTt2670(iroD)
SEXSTBHUCCB_28100
SENTTY21A_13520
STMSTM2775(iroD)
SEOSTM14_3346(iroD)
SEVSTMMW_27421
SEYSL1344_2760(iroD)
SEMSTMDT12_C28290
SEJSTMUK_2763(iroD)
SEBSTM474_2910(iroD)
SEFUMN798_3011(iroD)
SETUSTU288_14050
SETCCFSAN001921_03205
SENRSTMDT2_26811(iroD)
SENDDT104_27771(iroD)
SENICY43_14510
SEENSE451236_19970
SPTSPA2631(iroD)
SEKSSPA2453
SPQSPAB_03446
SEISPC_2815(iroD)
SECSCH_2706(iroD)
SEHSeHA_C2951
SHBSU5_03262
SENHCFSAN002069_18390
SEEHSEEH1578_22690
SEESNSL254_A2970
SENNSN31241_38630
SEWSeSA_A2917
SEASeAg_B2887
SENSQ786_13320
SEDSeD_A3073
SEGASPUCDC_2753(iroD)
SETSEN2621(iroD)
SENAAU38_13295
SENOAU37_13305
SENVAU39_13305
SENQAU40_14970
SENLIY59_13880
SENJCFSAN001992_19660
SEECCFSAN002050_20160
SEEBSEEB0189_006305
SENBBN855_28080
SENEIA1_13245
SENCSEET0819_20685
SESSARI_00203
SALZEOS98_05675
SDYSDY_1024(iroD)
SDZAsd1617_01305
ECLEECNIH2_07805
ECLYLI62_07660
ECLOENC_19090
EHMAB284_18100
ECANCWI88_15320
ENKLOC22_17640
KPUKP1_3613(iroD) KP1_p026(iroD)
KPPA79E_1751
KPTVK055_5101(iroD)
KPOKPN2242_26216
KPNKBN49_pII0028(iroD)
EAEEAE_03280
EARCCG33324
RTGNCTC13098_04508(fes_1)
PGELG71_24430
YREHEC60_09575
IZHFEM41_21250
GQUAWC35_23160
RBADH2866_00500
SCLsce7910
SCUSCE1572_45460
MRMA7982_09369
CCGCCASEI_02415
CVTB843_11165
CDOCDOO_09680
CTEDCTEST_02160
CSTACSTAT_02075
CFKCFRA_03215
CPHOCPHO_11130
CPEGCPELA_09415(fes)
CGKCGERO_09135(fes)
CRLNCTC7448_01441(fes)
CPSOCPPEL_09590(fes)
CCYSSAMEA4530656_2495(fes)
CMATHBA49_08975
CPRPI6I69_06890
COKCOCCU_02350(fes1)
GBRGbro_0641
GITC6V83_06505
GAMGII34_07530
GPDGII33_06920
TSMASU32_11055
DTMBJL86_1508
PAUSNCTC13651_02261(fes)
PGOFSB84_13515
MGIKGO620_016730
 » show all
Reference
1  [PMID:16076215]
  Authors
Lin H, Fischbach MA, Liu DR, Walsh CT
  Title
In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes.
  Journal
J Am Chem Soc 127:11075-84 (2005)
DOI:10.1021/ja0522027
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.109
IUBMB Enzyme Nomenclature: 3.1.1.109
ExPASy - ENZYME nomenclature database: 3.1.1.109
BRENDA, the Enzyme Database: 3.1.1.109

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