KEGG ENZYME: 3.1.1.14

ENZYME: 3.1.1.14

Entry

EC 3.1.1.14                 Enzyme

Name

chlorophyllase;
CLH;
Chlase

Class

Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases

Sysname

chlorophyll chlorophyllidohydrolase

Reaction(IUBMB)

chlorophyll + H2O = phytol + chlorophyllide

Reaction(KEGG)

(other) R05618 R09068

Substrate

chlorophyll [CPD:C01793];
H2O [CPD:C00001]

Product

phytol [CPD:C01389];
chlorophyllide [CPD:C02139]

Comment

Chlorophyllase has been found in higher plants, diatoms, and in the green algae Chlorella [3]. This enzyme forms part of the chlorophyll degradation pathway and is thought to take part in de-greening processes such as fruit ripening, leaf senescence and flowering, as well as in the turnover and homeostasis of chlorophyll [4]. This enzyme acts preferentially on chlorophyll a but will also accept chlorophyll b and pheophytins as substrates [5]. Ethylene and methyl jasmonate, which are known to accelerate senescence in many species, can enhance the activity of the hormone-inducible form of this enzyme [5].

History

EC 3.1.1.14 created 1961, modified 2007

Pathway

ec00860

Porphyrin metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K08099

chlorophyllase

Genes

BFO:	118430150 118430151 118430600 118430728

BBEL:

109479793 109479795 109479796 109479797

DPX:	DAPPUDRAFT_109234 DAPPUDRAFT_307488

DMK:	116931126 116931128

PVM:	113808190 113820612

PJA:	122256068 122263317

PCHN:

125036948 125046224

PMOO:

119588577 119590394

HAME:

121854997

CQD:

128704775

PTRU:

123505794 123505801

ESN:	126994043 126995275

MNZ:

135212008

MRJ:

136852139

HAZT:

108682296 108683295

EAF:	111710345 111713932

TCF:

131880242

PPOI:

119090331

PVUL:

126823540 126824324

BGT:	106056453 106056471

HRF:	124141054 124141055 124141515

HRJ:	124256195 124256196 124265847 124281765

HASI:

137261732 137287306

CRG:

105346495

CANU:

128188310

CVN:

111133346

OED:

125663743

MYI:

110462918

PMAX:

117321073 117344891

AIRR:

138314926

MMER:

123550267

RPHI:

132717156

DPOL:

127845313 127845546 127845868 127846216

MAEA:

128244124

LJP:	135467231 135467255

LAK:

106178068

ATH:	AT1G19670(CLH1) AT5G43860(CLH2)

ALY:	9299705 9329122

CRB:	17877061 17898197

CSAT:

104725070 104725071 104740665 104756312 104760568 104760570 104771535 104772775 104776081

EUS:	EUTSA_v10003248mg EUTSA_v10008201mg

BRP:	103839225 103848843 103872768

BNA:	106346945 106366642 106407289 106416159 106451729 125592422

BOE:	106313945 106323049 106344030

RSZ:	108813624 108825985 108840190

THJ:

104801988

CPAP:

110815852

CIT:	102578008(CHLASE1) 102620504 102621972 127901493

CIC:	CICLE_v10005453mg CICLE_v10021095mg CICLE_v10021103mg CICLE_v10024279mg

PVY:	116136638 116145869

MINC:

123210567 123221132

TCC:

18603049

GRA:	105778393 105792273

GHI:	107887126 107914870 107936486 107940220

GAB:	108464995 108469438

HSYR:

120147021 120204241

DZI:	111291557 111298521 111306098 111313428

EGR:	104423825 104423837 104423847

GMX:	100790901 100807959 100808490 100820123

GSJ:	114367379 114372586 114418639 114418804

PVU:	137827577 137836539

VRA:	106756600 106768765 106771335

VAR:	108325428 108345122 108345314

VUN:	114181584 114182439 114189882

VUM:	124824349 124831168 124838183

CCAJ:

109788901 109795324

APRC:

113866769 113872403

MTR:	11437154 25482953 25482954

TPRA:

123887376 123892259 123893194 123893214 123898611 123902293 123902294 123902625

CAM:

101490133

PSAT:

127097048 127114269

VVO:	131598746 131598747 131600185 131626629

LJA:	130720918 130726141 130738840

ADU:	107463645 107493176

AIP:	107626511 107645208

AHF:	112696243 112729005 112759021 112761361

LANG:

109339525 109354986

PCIN:

129298141 129299954 129304613 129310125

QSA:	O6P43_010595 O6P43_017738 O6P43_021530

FVE:	101295614 101306192

AANS:

126784135 126784452 126791652

RCN:	112169488 112195827

PPER:

18768229 18792394

PMUM:

103323995 103334201

PAVI:

110763481 110768653

PDUL:

117614258 117637301

MDM:	103419096 103419118 103449043 103453222

MSYL:

126590478 126595922 126616700

PXB:	103935863 103960097

ZJU:	107423104 107426389

MNT:	21385927 21385928 21392462

CSAV:

115721858 115724631

CSV:	101203653 101206529

CMO:	103491233 103491250 103503349

BHJ:	120077598 120092495

MCHA:

111009609 111009779

CMAX:

111499382

CMOS:

111459924

CPEP:

111807242

RCU:

8280860

MEAN:

126666622 126686238 126688378

JCU:	105632037 105649942 105649943 105650221

HBR:	110632586 110672194 110672203 110673201

MESC:

110611886 110614412 110626942

POP:	18109913 7474174

PEU:	105130020 105131395 105131482

PALZ:

118061495 118061656 118061660

PNZ:	133694064 133694118 133696253

JRE:	108989432 109005075

CILL:

122278211 122300769

CAVE:

132168478 132169890 132172874 132178655

AGLU:

133858602 133868741 133868907

QSU:	111985871 112012335 112028542 136069502

QLO:	115950929 115970703

TWL:	119992824 120000993 120007192

VVI:	100243499 100244179 100248634 100249336 100252835 100853040

VRI:	117913783 117917855 117918826 117919171

SLY:	101254033 101258376 101263579 101265579

SPEN:

107005311 107021195 107030576

SOT:	102583310 102584485 102599165 102603471

SSTN:

125851858 125863323 125870968 125874432

SDUL:

129870438 129883038 129901192

CANN:

107853614 107873769 124897042

LBB:	132609766 132626757 132636469 132642467

NTA:	107767570 107795708 107812657 107817300 107820048 107822816

NSY:	104213060 104226899 104239014 104241943 104246002

NTO:	104085961 104093037

NAU:	109214525 109219777 109220450 109239633 109241853

INI:	109161293 109176252

ITR:	116001664 116030002

SIND:

105163609 105171067 105171304

OEU:	111371701 111377213 111396764

EGT:	105949808 105950913 105953292

SSPL:

121756166 121797626

SMIL:

130991408 130992773

SHIS:

125196609 125218330

APAN:

127247737 127258669

HAN:	110863854 110864074 110940214

ECAD:

122592936 122593774 122608201

LSV:	111894801 111894805

CCAV:

112506625 112507467

DCR:	108193833 108206898

CSIN:

114293959 114293960 114298031 114298882 114309888 114315681

RVL:	131309656 131314458

AEW:	130750838 130756700 130771133

BVG:	104886508 104907958

SOE:	110784312 110784373 110803699

CQI:	110684804 110699749 110702553 110702554 110719158 110719405

ATRI:

130803877 130825228

MOF:	131151759 131151760

NNU:	104596611 104596676

MING:

122059453 122088590 122088656 122090825

TSS:	122641795 122648733

PSOM:

113280354 113315788 113336762 113350749 113352605 113357756

OSA:

4348648

DOSA:

Os10g0419600

OBR:

102713942

OGL:

127753224

BDI:	100824120 100833871 100835336 100839712

ATS:	109772274 109772321 109776391 109783103

TDC:	119267169 119275018 119302851 119310060 119311545 119314261 119315090 119324830 119325076

TAES:

123057940 123064963 123077378 123094110 123104759 123125412 123125413 123129881 123130453 123133036 123139674 123144040

TUA:	125515469 125529299 125542870

LPER:

127331196 127336901

LRD:	124669524 124673350 124674659 124675755 124682008 124691054

SBI:	8057813 8059442 8062553

ZMA:	100191887 100383045 103632406

MFLO:

136501668 136501685 136502140 136502182 136551476 136551477

SITA:

101760397 101763039 101773232

SVS:	117836408 117842290 117849346 117866833

PVIR:

120651010 120658768 120671526 120679154

PHAI:

112873530 112880278 112896417

PAUA:

133897242 133902024 133907896

PDA:	103695966 103705552

EGU:	105053391 105054855

MUS:

103978829

ZOF:	121972176 122011636 122011637 122016014 122016437

DCT:	110115286 110115287

PEQ:

110032436

AOF:	109835385 109835452 109848662

MSIN:

131249378 131249379 131249381 131249382

ACAK:

137934808 137948430

NCOL:

116246305 116246306 116248862 116266274

ATR:	18429611 18429616

CJF:	131047479 131047482 131050734 131051696 131051699

SMO:	SELMODRAFT_107983 SELMODRAFT_176680 SELMODRAFT_57004 SELMODRAFT_66990 SELMODRAFT_85435 SELMODRAFT_86398

PPP:

112273217

» show all

Reference

1 [PMID:13715233]

Authors

HOLDEN M.

Title

The breakdown of chlorophyll by chlorophyllase.

Journal

Biochem J 78:359-64 (1961)
DOI:10.1042/bj0780359

Reference

2 [PMID:13756631]

Authors

KLEIN AO, VISHNIAC W.

Title

Activity and partial purification of chlorophyllase in aqueous systems.

Journal

J Biol Chem 236:2544-7 (1961)

Reference

3 [PMID:10611389]

Authors

Tsuchiya T, Ohta H, Okawa K, Iwamatsu A, Shimada H, Masuda T, Takamiya K

Title

Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate.

Journal

Proc Natl Acad Sci U S A 96:15362-7 (1999)
DOI:10.1073/pnas.96.26.15362

Sequence

[ag:AAF27045] [ath:AT1G19670 AT5G43860]

Reference

4 [PMID:16610227]

Authors

Okazawa A, Tango L, Itoh Y, Fukusaki E, Kobayashi A.

Title

Characterization and subcellular localization of chlorophyllase from Ginkgo biloba.

Journal

Z Naturforsch [C] 61:111-7 (2006)
DOI:10.1515/znc-2006-1-220

Reference

5 [PMID:16669755]

Authors

Hortensteiner S.

Title

Chlorophyll degradation during senescence.

Journal

Annu Rev Plant Biol 57:55-77 (2006)
DOI:10.1146/annurev.arplant.57.032905.105212

Other DBs

ExplorEnz - The Enzyme Database:

3.1.1.14

IUBMB Enzyme Nomenclature:

3.1.1.14

ExPASy - ENZYME nomenclature database:

3.1.1.14

BRENDA, the Enzyme Database:

3.1.1.14

CAS:

9025-96-1

DBGET integrated database retrieval system