KEGG   ENZYME: 3.1.1.76
Entry
EC 3.1.1.76                 Enzyme                                 

Name
poly(3-hydroxyoctanoate) depolymerase;
PHO depolymerase;
poly(3HO) depolymerase;
poly[(R)-hydroxyalkanoic acid] depolymerase;
poly(HA) depolymerase;
poly(HAMCL) depolymerase;
poly[(R)-3-hydroxyoctanoate] hydrolase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Sysname
poly{oxycarbonyl[(R)-2-pentylethylene]} hydrolase
Reaction(IUBMB)
Hydrolyses the polyester poly{oxycarbonyl[(R)-2-pentylethylene]} to oligomers [RN:R05796]
Reaction(KEGG)
R05796
Comment
The main product after prolonged incubation is the dimer [3]. Besides hydrolysing polymers of 3-hydroxyoctanoic acid, the enzyme also hydrolyses other polymers derived from medium-chain-length (C6-C12) hydroxyalkanoic acids and copolymers of mixtures of these. It also hydrolyses p-nitrophenyl esters of fatty acids. Polymers of short-chain-length hydroxyalkanoic acids such as poly[(R)-3-hydroxybutanoic acid] and poly[(R)-3-hydroxypentanoic acid] are not hydrolysed.
History
EC 3.1.1.76 created 2001, modified 2005
Orthology
K22249  poly(3-hydroxyoctanoate) depolymerase
K22250  poly(3-hydroxyoctanoate) depolymerase
Genes
LYJ: FKV23_00190
PAE: PA5057(phaD)
PAEV: N297_5230(phaZ)
PAEI: N296_5230(phaZ)
PAU: PA14_66830(phaD)
PAP: PSPA7_5795(phaZ)
PAG: PLES_54471(phaD)
PAF: PAM18_5172(phaD)
PNC: NCGM2_0454(phaD)
PAEB: NCGM1900_5813(phaD)
PAEP: PA1S_27405
PAEM: U769_27660
PAEL: T223_27865
PAEU: BN889_05615(phaD)
PAEG: AI22_06590
PAEC: M802_5228(phaZ)
PAEO: M801_5095(phaZ)
PMY: Pmen_0529
PMK: MDS_0567
PRE: PCA10_52780(phaZ)
PPSE: BN5_0413(phaZ)
PCQ: PcP3B5_05850(catD_2)
PMUI: G4G71_00390(phaZ)
PPU: PP_5004(phaB)
PPF: Pput_4878
PPT: PPS_4848
PPI: YSA_04109
PPX: T1E_5659(phaB)
PPUH: B479_24485
PPUT: L483_30175
PPUN: PP4_50710(phaZ)
PPUD: DW66_5239
PMON: X969_23945
PMOT: X970_23580
PST: PSPTO_5144(phaB)
PSYR: N018_23800
PSP: PSPPH_0378(phaZ)
PAMG: BKM19_003495(phaZ)
PAVL: BKM03_27060(phaZ)
PVD: CFBP1590__4931(phaB)
PFL: PFL_0433(phaZ)
PPRC: PFLCHA0_c04400(phaB)
PPRO: PPC_0447
PFE: PSF113_0412(phaZ)
PFS: PFLU_0395(phaB)
PFC: PflA506_0387(phaZ)
PFB: VO64_3471
PMAN: OU5_3070
PRX: HRH33_02910(phaZ)
PEN: PSEEN5067(phaZ)
PPUU: PputUW4_00332(phaZ1) PputUW4_02301(phaZ2)
PKC: PKB_0465(phaB)
PSES: PSCI_2219
PSEM: TO66_02110
PSEC: CCOS191_0483(phaB)
PSOS: POS17_0424
PANR: A7J50_0385
PSET: THL1_5319
PSIL: PMA3_01420
PKE: DLD99_02265(phaZ)
PALL: UYA_02425
POJ: PtoMrB4_52200(phaD)
PGY: AWU82_21810(phaZ)
PLAL: FXN65_25880(phaZ)
PSEJ: HNQ25_21875(phaZ)
PVK: EPZ47_01975(phaZ)
PBZ: GN234_16070(phaZ)
PKH: JLK41_02530(phaZ)
PTY: JWV26_13290(phaZ)
PMAO: PMYSY11_4140(phaZ)
PATA: JWU58_25445(phaZ)
PDW: BV82_2659(phaZ)
PZE: HU754_003075(phaZ)
PMOE: HV782_003005(phaZ)
SALN: SALB1_0347
BCT: GEM_3227
BUB: BW23_3668
BSEM: WJ12_32285
BMEC: WJ16_30770
BSTG: WT74_31100
PLG: NCTC10937_00592(phaZ)
PSTG: E8M01_05815(phaZ)
SECH: B18_10555
BBA: Bd3709
BBAT: Bdt_3599
BBAC: EP01_11030
PARH: I5S86_03190(phaZ)
PACC: PAC1_08325
PACH: PAGK_0599
MHU: Mhun_0431
 » show all
Reference
1  [PMID:11217416]
  Authors
Jendrossek D.
  Title
Microbial degradation of polyesters.
  Journal
Adv Biochem Eng Biotechnol 71:293-325 (2001)
DOI:10.1007/3-540-40021-4_10
Reference
2  [PMID:10506180]
  Authors
Garcia B, Olivera ER, Minambres B, Fernandez-Valverde M, Canedo LM, Prieto MA, Garcia JL, Martinez M, Luengo JM
  Title
Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon.
  Journal
J Biol Chem 274:29228-41 (1999)
DOI:10.1074/jbc.274.41.29228
  Sequence
Reference
3  [PMID:8476295]
  Authors
Schirmer A, Jendrossek D, Schlegel HG
  Title
Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13.
  Journal
Appl Environ Microbiol 59:1220-7 (1993)
DOI:10.1128/AEM.59.4.1220-1227.1993
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.76
IUBMB Enzyme Nomenclature: 3.1.1.76
ExPASy - ENZYME nomenclature database: 3.1.1.76
BRENDA, the Enzyme Database: 3.1.1.76

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