KEGG   ENZYME: 3.1.1.77
Entry
EC 3.1.1.77                 Enzyme                                 
Name
acyloxyacyl hydrolase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Reaction(IUBMB)
3-(acyloxy)acyl group of bacterial toxin + H2O = 3-hydroxyacyl group of bacterial toxin + a fatty acid [RN:R05792]
Reaction(KEGG)
R05792
Substrate
3-(acyloxy)acyl group of bacterial toxin [CPD:C11547];
H2O [CPD:C00001]
Product
3-hydroxyacyl group of bacterial toxin [CPD:C11548];
fatty acid [CPD:C00162]
Comment
The substrate is lipid A on the reducing end of the toxic lipopolysaccharide (LPS) of Salmonella typhimurium and related organisms. It consists of diglucosamine, beta-D-GlcN-(1-> 6)-D-GlcN, attached by glycosylation on O-6 of its non-reducing residue, phosphorylated on O-4 of this residue and on O-1 of its potentially reducing residue. Both residues carry 3-(acyloxy)acyl groups on N-2 and O-3. The enzyme from human leucocytes detoxifies the lipid by hydrolysing the secondary acyl groups from O-3 of the 3-hydroxyacyl groups on the disaccharide (LPS). It also possesses a wide range of phospholipase and acyltransferase activities [e.g. EC 3.1.1.4 (phospholipase A2), EC 3.1.1.5 (lysophospholipase), EC 3.1.1.32 (phospholipase A1) and EC 3.1.1.52 (phosphatidylinositol deacylase)], hydrolysing diacylglycerol and phosphatidyl compounds, but not triacylglycerols. It has a preference for saturated C12-C16 acyl groups.
History
EC 3.1.1.77 created 2001
Orthology
K01065  acyloxyacyl hydrolase
Genes
HSA313(AOAH)
PTR463352(AOAH)
PPS100994136(AOAH)
GGO101134942(AOAH)
PON100456260(AOAH)
NLE100585049(AOAH)
MCC706291(AOAH)
MCF102141551(AOAH)
MTHB126951433
CSAB103226220(AOAH)
CATY105596820(AOAH)
PANU101008469(AOAH)
TGE112620443(AOAH)
RRO104660689(AOAH)
RBB108530681(AOAH)
TFN117073092(AOAH)
PTEH111520348(AOAH)
CJC100388358(AOAH)
SBQ101038550(AOAH)
CSYR103265601(AOAH)
MMUR105878242(AOAH)
LCAT123646891(AOAH)
OGA100943005(AOAH)
MMU27052(Aoah)
MCAL110308210(Aoah)
MPAH110333962(Aoah)
RNO498757(Aoah)
MCOC116082376(Aoah)
MUN110563276(Aoah)
CGE100768833(Aoah)
MAUA101823247(Aoah)
PLEU114685851(Aoah)
MORG121443959(Aoah)
MFOT126501876
AAMP119816970(Aoah)
NGI103743893(Aoah)
HGL101718171(Aoah)
CPOC100714206(Aoah)
CCAN109698614(Aoah)
DORD106000820(Aoah)
DSP122120841(Aoah)
NCAR124991975
OCU100008662(AOAH)
OPI101524623(AOAH)
TUP102490537(AOAH)
CFA482402(AOAH)
CLUD112670726(AOAH)
VVP112909773(AOAH)
VLG121474477(AOAH)
AML100471433(AOAH)
UMR103658989(AOAH)
UAH113262793(AOAH)
UAR123789068(AOAH)
ELK111147266
LLV125081111
MPUF101691175(AOAH)
ORO101377931(AOAH)
EJU114201299(AOAH) 114202987 114202988
ZCA113911606(AOAH)
MLX118020968(AOAH)
NSU110588897(AOAH)
FCA101101376(AOAH)
PYU121028485(AOAH)
PBG122488103(AOAH)
LRUF124523104
PTG102960989(AOAH)
PPAD109248427(AOAH)
AJU106968650(AOAH)
HHV120236380(AOAH)
BTA768208(AOAH)
BOM102282514(AOAH)
BIU109557445(AOAH)
BBUB102416197(AOAH)
CHX102189546(AOAH)
OAS101115787
ODA120877794(AOAH)
CCAD122438382(AOAH)
SSC100522290(AOAH)
CFR102523361(AOAH)
CBAI105069301(AOAH)
CDK105095876(AOAH)
VPC102531274(AOAH)
BACU103014073(AOAH)
LVE103075940(AOAH)
OOR101290037(AOAH)
DLE111179540(AOAH)
PCAD102983172(AOAH)
PSIU116759016(AOAH)
ECB100055508(AOAH)
EPZ103561558(AOAH)
EAI106830867(AOAH)
MYB102260205(AOAH)
MYD102763366(AOAH)
MMYO118665926(AOAH)
MLF102420935(AOAH)
MNA107544368(AOAH)
PKL118727848(AOAH)
HAI109389807(AOAH)
DRO112306988(AOAH)
SHON118983525(AOAH)
AJM119035213(AOAH)
PDIC114508104(AOAH)
PHAS123811540(AOAH)
MMF118620246(AOAH)
RFQ117012105(AOAH)
PALE102893901(AOAH)
PGIG120602453(AOAH)
PVP105293694(AOAH)
RAY107518274(AOAH)
MJV108397845(AOAH)
TOD119244517(AOAH)
SARA101554919(AOAH)
LAV100655055(AOAH)
TMU101341268
DNM101425928
MDO103104605(AOAH)
SHR100913760(AOAH)
PCW110192926(AOAH)
OAA103167871(AOAH)
GGA420737(AOAH)
PCOC116227247(AOAH)
MGP100548243(AOAH)
CJO107309429(AOAH)
NMEL110393524(AOAH)
APLA101793271(AOAH)
ACYG106044351(AOAH)
AFUL116485581(AOAH)
TGU100230185(AOAH)
LSR110483388(AOAH)
SCAN103821110(AOAH)
PMOA120505053(AOAH)
OTC121337621(AOAH)
PRUF121352993(AOAH)
GFR102036558(AOAH)
FAB101820983(AOAH)
PHI102111626(AOAH)
PMAJ107200241(AOAH)
CCAE111923611(AOAH) 111923900
CCW104688946(AOAH)
CBRC103615772(AOAH)
ETL114061499(AOAH)
ZAB102073705(AOAH)
ACHL103797429(AOAH)
FPG101918125(AOAH)
FCH102059809(AOAH)
CLV102091359(AOAH)
EGZ104124826(AOAH)
NNI104012086(AOAH)
PLET104625819(AOAH)
PCRI104036962(AOAH)
ACUN113477589(AOAH)
TALA104362821(AOAH)
PADL103923970(AOAH)
AFOR103894143(AOAH)
ACHC115339300(AOAH)
HALD104325017(AOAH)
CCRI104160793(AOAH)
CSTI104561911(AOAH)
EHS104514242(AOAH)
CMAC104483455(AOAH)
MUI104540411(AOAH)
FGA104080337 104081074(AOAH)
GSTE104262346(AOAH)
LDI104350203(AOAH)
OHA104328649(AOAH)
NNT104405721(AOAH)
DPUB104299664(AOAH)
PGUU104469217(AOAH)
ACAR104525678(AOAH)
AVIT104272248 104275868(AOAH)
CVF104282002(AOAH)
AAM106489028(AOAH)
AROW112974608(AOAH)
NPD112948572(AOAH)
TGT104573647(AOAH)
DNE112984341(AOAH)
SCAM104147189(AOAH)
ASN102373583(AOAH)
AMJ102570124(AOAH)
CPOO109312609(AOAH)
GGN109291847(AOAH)
PSS102453660(AOAH)
CMY102943071(AOAH)
CPIC101940255(AOAH)
TST117872575(AOAH)
CABI116827694(AOAH)
MRV120398882(AOAH)
ACS100554361(aoah)
PVT110076184(AOAH)
SUND121934577(AOAH)
PBI103067406
PMUR107283808(AOAH)
CTIG120305260(AOAH)
VKO123035856(AOAH)
PMUA114607810(AOAH)
ZVI118091936(AOAH)
GJA107117166(AOAH)
STOW125440991(AOAH)
XLA108719016(aoah.L)
XTR100491792(aoah)
NPR108792217(AOAH)
RTEM120940393(AOAH)
BBUF121001980(AOAH)
BGAR122937882(AOAH)
BFO118418622
BBEL109480518
CIN100179746
SCLV120332962
SPU592185
SKO102806049 102806164
TCA657467
ATD109598277
AGB108909550
NLU111062664
FOC113218019
TPAL117646427
ZNE110828349
CSEC111863447
EAF111702609
CSCU111615746 111633112
PTEP107441857
SDM118187617
LGILOTGIDRAFT_164760
PCAN112577321
BGT106061699
GAE121389307 121389308
HRF124140897
HRJ124287899
CRG105332002
MYI110448174 110448175
PMAX117337110
MMER123549627
LAK106172532
NVE5502616
EPA110241226
ATEN116294129
ADF107349171
AMIL114959881
PDAM113685157
SPIS111323837
DGT114522567
XEN124459550
AQU100634015
MBRMONBRDRAFT_34989
SREPTSG_01302
DDIDDB_G0272785
DPPDICPUDRAFT_153563
DFADFA_01937 DFA_03128
ACANACA1_113860 ACA1_253120
TETTTHERM_00535580 TTHERM_00563920
TBRTb10.70.2420
TBGTbgDal_X5820
TCR510289.40
NGRNAEGRDRAFT_80170 NAEGRDRAFT_82645
 » show all
Reference
1  [PMID:2398058]
  Authors
Erwin AL, Munford RS.
  Title
Deacylation of structurally diverse lipopolysaccharides by human acyloxyacyl hydrolase.
  Journal
J Biol Chem 265:16444-9 (1990)
Reference
2  [PMID:1883828]
  Authors
Hagen FS, Grant FJ, Kuijper JL, Slaughter CA, Moomaw CR, Orth K, O'Hara PJ, Munford RS
  Title
Expression and characterization of recombinant human acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides.
  Journal
Biochemistry 30:8415-23 (1991)
DOI:10.1021/bi00098a020
  Sequence
[hsa:313]
Reference
3  [PMID:1577781]
  Authors
Munford RS, Hunter JP.
  Title
Acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides, has phospholipase, lysophospholipase, diacylglycerollipase, and acyltransferase activities in vitro.
  Journal
J Biol Chem 267:10116-21 (1992)
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.77
IUBMB Enzyme Nomenclature: 3.1.1.77
ExPASy - ENZYME nomenclature database: 3.1.1.77
BRENDA, the Enzyme Database: 3.1.1.77
CAS: 110277-64-0

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