EC                 Enzyme                                 

acyloxyacyl hydrolase
Acting on ester bonds;
Carboxylic-ester hydrolases
3-(acyloxy)acyl group of bacterial toxin + H2O = 3-hydroxyacyl group of bacterial toxin + a fatty acid [RN:R05792]
3-(acyloxy)acyl group of bacterial toxin [CPD:C11547];
H2O [CPD:C00001]
3-hydroxyacyl group of bacterial toxin [CPD:C11548];
fatty acid [CPD:C00162]
The substrate is lipid A on the reducing end of the toxic lipopolysaccharide (LPS) of Salmonella typhimurium and related organisms. It consists of diglucosamine, beta-D-GlcN-(1-> 6)-D-GlcN, attached by glycosylation on O-6 of its non-reducing residue, phosphorylated on O-4 of this residue and on O-1 of its potentially reducing residue. Both residues carry 3-(acyloxy)acyl groups on N-2 and O-3. The enzyme from human leucocytes detoxifies the lipid by hydrolysing the secondary acyl groups from O-3 of the 3-hydroxyacyl groups on the disaccharide (LPS). It also possesses a wide range of phospholipase and acyltransferase activities [e.g. EC (phospholipase A2), EC (lysophospholipase), EC (phospholipase A1) and EC (phosphatidylinositol deacylase)], hydrolysing diacylglycerol and phosphatidyl compounds, but not triacylglycerols. It has a preference for saturated C12-C16 acyl groups.
EC created 2001
K01065  acyloxyacyl hydrolase
HSA: 313(AOAH)
PTR: 463352(AOAH)
PPS: 100994136(AOAH)
GGO: 101134942(AOAH)
PON: 100456260(AOAH)
NLE: 100585049(AOAH)
MCC: 706291(AOAH)
MCF: 102141551(AOAH)
CSAB: 103226220(AOAH)
CATY: 105596820(AOAH)
PANU: 101008469(AOAH)
RRO: 104660689(AOAH)
RBB: 108530681(AOAH)
TFN: 117073092(AOAH)
PTEH: 111520348(AOAH)
CJC: 100388358(AOAH)
SBQ: 101038550(AOAH)
MMUR: 105878242(AOAH)
MMU: 27052(Aoah)
MCAL: 110308210(Aoah)
MPAH: 110333962(Aoah)
RNO: 498757(Aoah)
MCOC: 116082376(Aoah)
MUN: 110563276(Aoah)
CGE: 100768833(Aoah)
PLEU: 114685851(Aoah)
NGI: 103743893(Aoah)
HGL: 101718171(Aoah)
CCAN: 109698614(Aoah)
OCU: 100008662(AOAH)
TUP: 102490537(AOAH)
CFA: 482402(AOAH)
VVP: 112909773(AOAH)
VLG: 121474477(AOAH)
AML: 100471433(AOAH)
UMR: 103658989(AOAH)
UAH: 113262793(AOAH)
ORO: 101377931(AOAH)
ELK: 111147266
MPUF: 101691175(AOAH)
MLX: 118020968(AOAH)
FCA: 101101376(AOAH)
PYU: 121028485(AOAH)
PTG: 102960989(AOAH)
PPAD: 109248427(AOAH)
AJU: 106968650(AOAH)
HHV: 120236380(AOAH)
BTA: 768208(AOAH)
BOM: 102282514(AOAH)
BIU: 109557445(AOAH)
BBUB: 102416197(AOAH)
CHX: 102189546(AOAH)
OAS: 101115787
ODA: 120877794(AOAH)
CCAD: 122438382(AOAH)
SSC: 100522290(AOAH)
CFR: 102523361(AOAH)
CBAI: 105069301(AOAH)
CDK: 105095876(AOAH)
BACU: 103014073(AOAH)
LVE: 103075940(AOAH)
OOR: 101290037(AOAH)
DLE: 111179540(AOAH)
PCAD: 102983172(AOAH)
ECB: 100055508(AOAH)
EPZ: 103561558(AOAH)
EAI: 106830867(AOAH)
MYB: 102260205(AOAH)
MYD: 102763366(AOAH)
MMYO: 118665926(AOAH)
MNA: 107544368(AOAH)
HAI: 109389807(AOAH)
DRO: 112306988(AOAH)
SHON: 118983525(AOAH)
AJM: 119035213(AOAH)
MMF: 118620246(AOAH)
PALE: 102893901(AOAH)
PGIG: 120602453(AOAH)
RAY: 107518274(AOAH)
MJV: 108397845(AOAH)
TOD: 119244517(AOAH)
LAV: 100655055(AOAH)
TMU: 101341268
MDO: 103104605(AOAH)
SHR: 100913760(AOAH)
PCW: 110192926(AOAH)
OAA: 103167871(AOAH)
GGA: 420737(AOAH)
PCOC: 116227247(AOAH)
MGP: 100548243(AOAH)
CJO: 107309429(AOAH)
NMEL: 110393524(AOAH)
APLA: 101793271(AOAH)
ACYG: 106044351(AOAH)
TGU: 100230185(AOAH)
LSR: 110483388(AOAH)
SCAN: 103821110(AOAH)
PMOA: 120505053(AOAH)
GFR: 102036558(AOAH)
FAB: 101820983(AOAH)
PHI: 102111626(AOAH)
PMAJ: 107200241(AOAH)
CCAE: 111923611(AOAH) 111923900
CCW: 104688946(AOAH)
ETL: 114061499(AOAH)
FPG: 101918125(AOAH)
FCH: 102059809(AOAH)
CLV: 102091359(AOAH)
EGZ: 104124826(AOAH)
NNI: 104012086(AOAH)
ACUN: 113477589(AOAH)
PADL: 103923970(AOAH)
AAM: 106489028(AOAH)
NPD: 112948572(AOAH)
ASN: 102373583(AOAH)
AMJ: 102570124(AOAH)
CPOO: 109312609(AOAH)
GGN: 109291847(AOAH)
PSS: 102453660(AOAH)
CMY: 102943071(AOAH)
CPIC: 101940255(AOAH)
TST: 117872575(AOAH)
CABI: 116827694(AOAH)
ACS: 100554361(aoah)
PVT: 110076184(AOAH)
PBI: 103067406
PMUR: 107283808(AOAH)
PMUA: 114607810(AOAH)
ZVI: 118091936(AOAH)
GJA: 107117166(AOAH)
XLA: 108719016(aoah.L)
XTR: 100491792(aoah)
NPR: 108792217(AOAH)
BFO: 118418622
BBEL: 109480518
CIN: 100179746
SCLV: 120332962
SPU: 592185
TCA: 657467
ATD: 109598277
AGB: 108909550
NLU: 111062664
ZNE: 110828349
CSEC: 111863447
EAF: 111702609
PTEP: 107441857
SDM: 118187617
PCAN: 112577321
BGT: 106061699
CRG: 105332002
PMAX: 117337110
LAK: 106172532
NVE: 5502616
EPA: 110241226
ATEN: 116294129
ADF: 107349171
AMIL: 114959881
PDAM: 113685157
SPIS: 111323837
DGT: 114522567
AQU: 100634015
TCR: 510289.40
 » show all
1  [PMID:2398058]
Erwin AL, Munford RS.
Deacylation of structurally diverse lipopolysaccharides by human acyloxyacyl hydrolase.
J Biol Chem 265:16444-9 (1990)
2  [PMID:1883828]
Hagen FS, Grant FJ, Kuijper JL, Slaughter CA, Moomaw CR, Orth K, O'Hara PJ, Munford RS
Expression and characterization of recombinant human acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides.
Biochemistry 30:8415-23 (1991)
3  [PMID:1577781]
Munford RS, Hunter JP.
Acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides, has phospholipase, lysophospholipase, diacylglycerollipase, and acyltransferase activities in vitro.
J Biol Chem 267:10116-21 (1992)
Other DBs
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IUBMB Enzyme Nomenclature:
ExPASy - ENZYME nomenclature database:
BRENDA, the Enzyme Database:
CAS: 110277-64-0

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