KEGG   ENZYME: 3.1.1.80Help
Entry
EC 3.1.1.80                 Enzyme                                 

Name
acetylajmaline esterase;
AAE;
2beta(R)-17-O-acetylajmalan:acetylesterase;
acetylajmalan esterase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
17-O-acetylajmaline O-acetylhydrolase
Reaction(IUBMB)
(1) 17-O-acetylajmaline + H2O = ajmaline + acetate [RN:R07513];
(2) 17-O-acetylnorajmaline + H2O = norajmaline + acetate [RN:R05880]
Reaction(KEGG)
Substrate
17-O-acetylajmaline [CPD:C15985];
H2O [CPD:C00001];
17-O-acetylnorajmaline [CPD:C11809]
Product
ajmaline [CPD:C06542];
acetate [CPD:C00033];
norajmaline [CPD:C11810]
Comment
This plant enzyme is responsible for the last stages in the biosynthesis of the indole alkaloid ajmaline. The enzyme is highly specific for the substrates 17-O-acetylajmaline and 17-O-acetylnorajmaline as the structurally related acetylated alkaloids vinorine, vomilenine, 1,2-dihydrovomilenine and 1,2-dihydroraucaffricine cannot act as substrates [2]. This is a novel member of the GDSL family of serine esterases/lipases.
History
EC 3.1.1.80 created 2006
Pathway
ec00901  Indole alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K21026  acetylajmaline esterase
Genes
RSS: 109442660
ATH: AT1G28580 AT1G28590 AT1G28600
ALY: ARALYDRAFT_336556 ARALYDRAFT_473078 ARALYDRAFT_668375 ARALYDRAFT_680622 ARALYDRAFT_680624 ARALYDRAFT_908337 ARALYDRAFT_916856
CRB: 111828597 111830104 111831227 111832504 111832505 111832506 111832507 111832508 111832509 111832587 111832593 111832656 17876944 17882068
CSAT: 104707391 104708668 104719419 104728539 104737095 104741468 104741471 104741477 104741761 104741762 104741797 104743589 104743590 104743592 104757205 104757206 104757207 104757212 104757213 104757563 104757811 104759479 104759480 104759481 104772876 104776920 104776922 104777081 104777193 104778923 104778924 104778925 104778926 109125022 109130222 109132262
EUS: EUTSA_v10006895mg EUTSA_v10007853mg EUTSA_v10009438mg EUTSA_v10009717mg EUTSA_v10015547mg
BRP: 103828994 103828998 103828999 103829000 103835308 103835309 103838941 103838944 103864749
BNA: 106355176 106358062 106358063 106358064 106358067 106361670 106361671 106370015 106394898 106396299 106396300 106397338 106397516 106421972 106421974 106433844 106448671 106449271 111197709 111204683 111213295
BOE: 106292724 106302168 106302199 106306085 106306088 106315364 106321476 106329340 106331440 106341599 106343864
THJ: 104798417 104801275 104814239 104814240 104826531
LJA: Lj0g3v0257729.1(Lj0g3v0257729.1) Lj6g3v1995900.1(Lj6g3v1995900.1) Lj6g3v1995900.2(Lj6g3v1995900.2) Lj6g3v1995910.1(Lj6g3v1995910.1)
DOSA: Os01t0214600-01(Os01g0214600) Os01t0215000-01(Os01g0215000) Os01t0215700-01(Os01g0215700) Os01t0216400-00(Os01g0216400) Os01t0216500-00(Os01g0216500) Os01t0216900-00(Os01g0216900) Os01t0223000-01(Os01g0223000) Os01t0649200-01(Os01g0649200) Os01t0649400-00(Os01g0649400) Os01t0649900-01(Os01g0649900) Os01t0650200-01(Os01g0650200) Os01t0650900-00(Os01g0650900) Os01t0651000-01(Os01g0651000) Os02t0604000-00(Os02g0604000) Os02t0608801-00(Os02g0608801) Os03t0365000-00(Os03g0365000) Os03t0365800-01(Os03g0365800) Os03t0365900-01(Os03g0365900) Os05t0209600-01(Os05g0209600) Os05t0210100-01(Os05g0210100) Os05t0210400-00(Os05g0210400) Os05t0506500-00(Os05g0506500) Os05t0506600-00(Os05g0506600) Os06t0156600-01(Os06g0156600) Os06t0157000-01(Os06g0157000) Os06t0694200-01(Os06g0694200) Os07t0642200-01(Os07g0642200) Os10t0393600-00(Os10g0393600) Os10t0393800-01(Os10g0393800) Os10t0394000-01(Os10g0394000) Os11t0521000-01(Os11g0521000)
ATS: 109732539(LOC109732539) 109733106(LOC109733106) 109733108(LOC109733108) 109733109(LOC109733109) 109733111(LOC109733111) 109733876(LOC109733876) 109735703(LOC109735703) 109737836(LOC109737836) 109738812(LOC109738812) 109738813(LOC109738813) 109739194(LOC109739194) 109740060(LOC109740060) 109740480(LOC109740480) 109744384(LOC109744384) 109749898(LOC109749898) 109752266(LOC109752266) 109752451(LOC109752451) 109752461(LOC109752461) 109754958(LOC109754958) 109754960(LOC109754960) 109754967(LOC109754967) 109755023(LOC109755023) 109755025(LOC109755025) 109755030(LOC109755030) 109756401(LOC109756401) 109756443(LOC109756443) 109756639(LOC109756639) 109756981(LOC109756981) 109760236(LOC109760236) 109762707(LOC109762707) 109762719(LOC109762719) 109763450(LOC109763450) 109763796(LOC109763796) 109766488(LOC109766488) 109766493(LOC109766493) 109768093(LOC109768093) 109768677(LOC109768677) 109768679(LOC109768679) 109769318(LOC109769318) 109770611(LOC109770611) 109770612(LOC109770612) 109770797(LOC109770797) 109771041(LOC109771041) 109771283(LOC109771283) 109772848(LOC109772848) 109773388(LOC109773388) 109773650(LOC109773650) 109776349(LOC109776349) 109776350(LOC109776350) 109776351(LOC109776351) 109776352(LOC109776352) 109776738(LOC109776738) 109776739(LOC109776739) 109777174(LOC109777174) 109778302(LOC109778302) 109779234(LOC109779234) 109779544(LOC109779544) 109779548(LOC109779548) 109781428(LOC109781428) 109781860(LOC109781860) 109782174(LOC109782174) 109783948(LOC109783948) 109785760(LOC109785760) 109785762(LOC109785762) 109785923(LOC109785923)
PPP: 112279097
 » show all
Taxonomy
Reference
1  [PMID:2955586]
  Authors
Polz L, Schubel H, Stockigt J.
  Title
Characterization of 2 beta (R)-17-O-acetylajmalan: acetylesterase--a specific enzyme involved in the biosynthesis of the Rauwolfia alkaloid ajmaline.
  Journal
Z Naturforsch [C] 42:333-42 (1987)
Reference
2  [PMID:16133216]
  Authors
Ruppert M, Woll J, Giritch A, Genady E, Ma X, Stockigt J.
  Title
Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.
  Journal
Planta 222:888-98 (2005)
DOI:10.1007/s00425-005-0031-0
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.80
IUBMB Enzyme Nomenclature: 3.1.1.80
ExPASy - ENZYME nomenclature database: 3.1.1.80
BRENDA, the Enzyme Database: 3.1.1.80
CAS: 110183-46-5

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