KEGG ENZYME: 3.1.1.83

ENZYME: 3.1.1.83

Entry

EC 3.1.1.83                 Enzyme

Name

monoterpene epsilon-lactone hydrolase;
MLH

Class

Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases

Sysname

isoprop(en)ylmethyloxepan-2-one lactonohydrolase

Reaction(IUBMB)

(1) isoprop(en)ylmethyloxepan-2-one + H2O = 6-hydroxyisoprop(en)ylmethylhexanoate (general reaction);
(2) 4-isopropenyl-7-methyloxepan-2-one + H2O = 6-hydroxy-3-isopropenylheptanoate [RN:R06390 R06392];
(3) 7-isopropyl-4-methyloxepan-2-one + H2O = 6-hydroxy-3,7-dimethyloctanoate [RN:R08979]

Reaction(KEGG)

R06390 R06392 R08979;
(other) R03751 R06391 R06393

Substrate

isoprop(en)ylmethyloxepan-2-one;
H2O [CPD:C00001];
4-isopropenyl-7-methyloxepan-2-one [CPD:C11414];
7-isopropyl-4-methyloxepan-2-one [CPD:C18066]

Product

6-hydroxyisoprop(en)ylmethylhexanoate;
6-hydroxy-3-isopropenylheptanoate [CPD:C11417];
6-hydroxy-3,7-dimethyloctanoate [CPD:C18067]

Comment

The enzyme catalyses the ring opening of epsilon-lactones which are formed during degradation of dihydrocarveol by the Gram-positive bacterium Rhodococcus erythropolis DCL14. The enzyme also acts on ethyl caproate, indicating that it is an esterase with a preference for lactones (internal cyclic esters). The enzyme is not stereoselective.

History

EC 3.1.1.83 created 2008

Pathway

ec00903

Limonene degradation

ec00930

Caprolactam degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K14731

epsilon-lactone hydrolase

Genes

PPJ:	RK21_02866

ACIC:

IOD65_14190

PPAI:

E1956_37465

POL:

Bpro_5566

ATEM:

PQV96_11840

HCZ:	G9Q37_07250

DIS:	I3K84_12425

MLO:

mll1869

MLN:	A9174_17235

MOP:	Mesop_3308

MAM:	Mesau_03191

MHUA:

MCHK_3437

MJR:	EB229_17475

MERD:

EB233_14750

RJG:	CCGE525_02335

RLN:	J0663_02145

RBAN:

J2J98_14760

RHIB:

I8E17_06385

KAI:

K32_23640

PSCQ:

KHQ08_18415

LABT:

FIU93_30965(mlhB)

CAK:

Caul_5125

RUT:	FIU92_22375(mlhB)

PHAE:

N1037_21380

CON:	TQ29_18790 TQ29_18930

SUAM:

BOO69_22540

SULD:

B5M07_18400

SULP:

AADW22_15340

SPSE:

SULPSESMR1_04141(mlhB)

MALU:

KU6B_59960

LVS:	LOKVESSMR4R_03943(mlhB)

SPHD:

HY78_29685

SBIN:

SBA_ch1_21760

SPCL:

F9288_06105

SBAR:

H5V43_03345

SPAG:

sphantq_03288(mlhB_1) sphantq_04480(mlhB_2)

PALJ:

LZC94_10710

NKO:	Niako_0270

MSA:	Mycsm_06641

MMC:

Mmcs_1513

MKM:

Mkms_1536

MFJ:	MFLOJ_25420

MSTO:

MSTO_28640

MSIM:

MSIM_15720

MSAK:

MSAS_01440

MKU:	I2456_11355

MPAA:

MKK62_01580

MSM:	MSMEG_3010

MSG:	MSMEI_2935(lipR)

MSB:	LJ00_14980

MSN:	LI99_14985

MSH:	LI98_14990

MVQ:	MYVA_1608 MYVA_4988

MCHT:

MCHIJ_02190

MMON:

EWR22_07700

MGO:	AFA91_24835

MDF:	K0O62_05005

MPAT:

MPNTM1_02123

CCOE:

CETAM_13035(mlhB)

NFR:	ERS450000_00738(mlhB_1)

NNO:	NONO_c26760(bah)

RHA:	RHA1_ro06680

RPY:	Y013_12975

RRZ:	CS378_11575 CS378_11785

RKO:	JWS14_15895 JWS14_20950 JWS14_43755

RGO:	KYT97_23850

ROZ:	CBI38_25685

RPSK:

JWS13_28455 JWS13_31000 JWS13_42110 JWS13_42175 JWS13_43350

RHOO:

RA302_30930

RHOJ:

JVH1_02765

RHOS:

QWW67_23460

RHOQ:

QWW98_23470

RHJS:

OYT95_22945 OYT95_35445

RHZQ:

GO592_34175

RHPD:

U9J23_25905

PDEF:

P9209_05620

GOR:

KTR9_3184

GTA:	BCM27_16265

GOD:	GKZ92_20420 GKZ92_20525

SGRF:

SGFS_010390

SCB:	SCAB_77011

SBH:

SBI_01199

SLS:	SLINC_0027

SDX:	C4B68_05970

SAQU:

EJC51_02305

SDEC:

L3078_00625 L3078_03500

SAKB:

K1J60_39635 K1J60_42320

SCAE:

IHE65_45115

SJN:	RI060_17530

SCOA:

QU709_03220 QU709_42465

KBU:	Q4V64_50825

STRL:

HEP84_49955

ABRY:

NYE86_02710

MHOS:

CXR34_00665

MSF:	IT882_15885

PSAI:

C3B54_111350

HEA:	HL652_02370

GMN:	GMOLON4_1493(chnC)

ARTP:

E5206_14690

GMY:

XH9_01110

SPUE:

AB5L97_16920

JAY:	H7A72_06395

BSPO:

L1F31_01650 L1F31_01865 L1F31_11510

MICG:

GJV80_04835

KSL:	OG809_40790

AGRA:

AGRA3207_001538

AMAZ:

LUW76_44795

ACTQ:

OG417_03105

SACG:

FDZ84_29665

AMQ:	AMETH_2426 AMETH_3877

AAB:	A4R43_22535

AMYY:

YIM_32910(mlhB3)

ATHM:

L1857_19155

AMYD:

K1T34_46470

PDX:

Psed_0489

PSEQ:

AD006_28260

PECQ:

AD017_28655

PAUT:

Pdca_39560

PBRO:

HOP40_29310

PPEL:

H6H00_18840 H6H00_19435 H6H00_19590

PMAD:

BAY61_09880 BAY61_19415

ACTY:

OG774_12490 OG774_29150

AIH:	Aiant_00540

ACTE:

ACTI_51590

AFS:

AFR_22850

DVC:	Dvina_29365

DAUR:

Daura_17400

DMAT:

Dmats_24580

SNA:

Snas_2426

EKE:	EK0264_02330

BSOL:

FSW04_20600

SBAE:

DSM104329_02046(mlhB_1)

» show all

Reference

1 [PMID:11157238]

Authors

van der Vlugt-Bergmans CJ, van der Werf MJ

Title

Genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from Rhodococcus erythropolis DCL14.

Journal

Appl Environ Microbiol 67:733-41 (2001)
DOI:10.1128/AEM.67.2.733-741.2001

Sequence

[ag:CAC17806]

Other DBs

ExplorEnz - The Enzyme Database:

3.1.1.83

IUBMB Enzyme Nomenclature:

3.1.1.83

ExPASy - ENZYME nomenclature database:

3.1.1.83

BRENDA, the Enzyme Database:

3.1.1.83

All links

Gene (13870)   
   MGENES (11593)   
   RefGene (2277)   
Protein sequence (583)   
   UniProt (581)   
   SWISS-PROT (1)   
   RefSeq(pep) (1)   
DNA sequence (721)   
   RefSeq(nuc) (2)   
   GenBank (362)   
   EMBL (357)   
Protein domain (3)   
   InterPro (3)   
All databases (15177)   

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