KEGG   ENZYME: 3.1.1.83
Entry
EC 3.1.1.83                 Enzyme                                 

Name
monoterpene epsilon-lactone hydrolase;
MLH
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Sysname
isoprop(en)ylmethyloxepan-2-one lactonohydrolase
Reaction(IUBMB)
(1) isoprop(en)ylmethyloxepan-2-one + H2O = 6-hydroxyisoprop(en)ylmethylhexanoate (general reaction);
(2) 4-isopropenyl-7-methyloxepan-2-one + H2O = 6-hydroxy-3-isopropenylheptanoate [RN:R06390 R06392];
(3) 7-isopropyl-4-methyloxepan-2-one + H2O = 6-hydroxy-3,7-dimethyloctanoate [RN:R08979]
Reaction(KEGG)
Substrate
isoprop(en)ylmethyloxepan-2-one;
H2O [CPD:C00001];
4-isopropenyl-7-methyloxepan-2-one [CPD:C11414];
7-isopropyl-4-methyloxepan-2-one [CPD:C18066]
Product
6-hydroxyisoprop(en)ylmethylhexanoate;
6-hydroxy-3-isopropenylheptanoate [CPD:C11417];
6-hydroxy-3,7-dimethyloctanoate [CPD:C18067]
Comment
The enzyme catalyses the ring opening of epsilon-lactones which are formed during degradation of dihydrocarveol by the Gram-positive bacterium Rhodococcus erythropolis DCL14. The enzyme also acts on ethyl caproate, indicating that it is an esterase with a preference for lactones (internal cyclic esters). The enzyme is not stereoselective.
History
EC 3.1.1.83 created 2008
Pathway
ec00903  Limonene and pinene degradation
ec00930  Caprolactam degradation
Orthology
K14731  epsilon-lactone hydrolase
Genes
QSU: 112008051
PFY: PFICI_02360
BZE: COCCADRAFT_39010
PFJ: MYCFIDRAFT_210278
BCOM: BAUCODRAFT_239045
GTT: GUITHDRAFT_118216
NGR: NAEGRDRAFT_61429
ESA: ESA_02202
CSK: ES15_2347
CSJ: CSK29544_03486
CAMA: F384_09330
SRL: SOD_c22360(bah)
SPLY: Q5A_012510(mlhB)
SOF: NCTC11214_04780(mlhB)
DDQ: DDI_1172
SOD: Sant_2259
EBI: EbC_28410
PSHI: SAMEA2665130_1587(mlhB)
XCA: xcc-b100_0088(aes)
VSP: VS_II0230
VTA: B0493
PPR: PBPRA1878(BP2097)
PAE: PA2098
PAEV: N297_2163(estE5)
PAEI: N296_2163(estE5)
PAEP: PA1S_15190
PAEM: U769_14790
PAEL: T223_16470
PAEC: M802_2160
PAEO: M801_2162
PMY: Pmen_1891
PMK: MDS_3007
PPSE: BN5_2548
PCQ: PcP3B5_22650(lipR)
PKC: PKB_1903
PANR: A7J50_3127
PALL: UYA_14495
ACC: BDGL_000404(bah)
MCUN: NCTC10297_01418(mlhB)
SON: SO_0616
SAZ: Sama_3012
SBL: Sbal_0570
SSE: Ssed_1377
SHL: Shal_2863
SKH: STH12_02924(mlhB)
MBS: MRBBS_1574(bah) MRBBS_2608(est)
MARJ: MARI_19160 MARI_20890(lipR_2)
AMAL: I607_17435
AMAE: I876_17815
AMAO: I634_03710
AMAD: I636_17685
AMAI: I635_18475
AMAG: I533_17380
AMAC: MASE_17505
AAUS: EP12_17950
GNI: GNIT_3308
FBL: Fbal_0749
FPZ: LA55_825
FPJ: LA02_1643
FNA: OOM_1385
TOL: TOL_3423
RSO: RSc0439
RSE: F504_463
RPI: Rpic_0315
BMA: BMA0171
BMAL: DM55_1576
BMAE: DM78_417
BMAQ: DM76_1555
BMAI: DM57_2208
BMAF: DM51_3147
BMAZ: BM44_905
BMAB: BM45_498
BPS: BPSL0623
BPSE: BDL_1380
BPSM: BBQ_2803
BPSU: BBN_2926
BPSD: BBX_3327
BPK: BBK_861
BPSH: DR55_469
BPSA: BBU_1527
BPSO: X996_76
BUT: X994_2091
BTQ: BTQ_562
BTZ: BTL_3159
BTD: BTI_3164
BTHA: DR62_1740
BTHL: BG87_2
BOK: DM82_52
BOC: BG90_969
BCN: Bcen_2135
BCEN: DM39_2836
BCEW: DM40_362
BAM: Bamb_2797
BMU: Bmul_0552
BMK: DM80_2170(estE5)
BCT: GEM_0695
BDL: AK34_343
BLAT: WK25_13310
BTEI: WS51_24105
BPSL: WS57_32480
BMEC: WJ16_14085
BSTG: WT74_14545
BGU: KS03_550
BGO: BM43_949
BPH: Bphy_0360
BFN: OI25_1075
PSPU: NA29_16340
CABA: SBC2_03610(aes_1) SBC2_64000(mlhB) SBC2_69740(aes_5) SBC2_76110(aes_6)
BPC: BPTD_0142
BPET: B1917_0149
BPEU: Q425_2600
BPA: BPP4011
AFQ: AFA_12560
RFR: Rfer_2416
POL: Bpro_5566
HYB: Q5W_20125
MMS: mma_2812
OFO: BRW83_0610(lipR)
DSU: Dsui_1821
AOA: dqs_2491
GEB: GM18_3868
PPD: Ppro_3541
DAS: Daes_1132
DWD: DSCW_01020(bah_1) DSCW_12170 DSCW_36520(bah_2)
CCX: COCOR_04675(bah)
SCL: sce9229
PACA: ID47_12025
MES: Meso_2945
RLE: RL1817
RLG: Rleg_1475
KAI: K32_23640
AOL: S58_03070
BRAD: BF49_4573
VGO: GJW-30_1_03242(lipR) GJW-30_1_03245(mlhB)
XAU: Xaut_4571
SNO: Snov_0624
META: Y590_20855
MTUN: MTUNDRAET4_1429(bah)
LABT: FIU93_30965(mlhB)
CSE: Cseg_0473
PZU: PHZ_c1044
TSV: DSM104635_00460(mlhB_1) DSM104635_00541(mlhB_2) DSM104635_01775(aes_3)
RUT: FIU92_22375(mlhB)
JAN: Jann_2666
DSH: Dshi_2588
OTM: OSB_05210(mlhB)
LAQU: R2C4_02585
CID: P73_3829
MALG: MALG_03045
RMM: ROSMUCSMR3_01282(mlhB)
RID: RIdsm_04322(lipR) RIdsm_04590(mlhB)
ROH: FIU89_18460(mlhB)
AHT: ANTHELSMS3_01617(mlhB)
MARU: FIU81_08240(lipF1) FIU81_15075(lipF2)
RCP: RCAP_rcc00461(est)
LVS: LOKVESSMR4R_03943(mlhB)
PAMO: BAR1_14975
GAK: X907_0626
NAR: Saro_3615
SPHP: LH20_19455
SPKC: KC8_10330
SECH: B18_22395
SSY: SLG_35930
SPHR: BSY17_3784
SPHT: K426_25054
SFLA: SPHFLASMR4Y_03196(mlhB)
AAY: WYH_00342(aes)
ELI: ELI_09720
ERF: FIU90_15335(lipF)
SCAP: AYP1020_2095(lipR_3)
PRI: PRIO_3266
AAC: Aaci_1055
AAD: TC41_1091
SPYA: A20_1103c
SPF: SpyM50796
SEZ: Sez_0446
SEQ: SZO_15360
SEQU: Q426_07040
SANS: DK43_02045
LRH: LGG_00065(aes)
LRL: LC705_00057(aes)
LRA: LRHK_62
LBH: Lbuc_0514
LBR: LVIS_0232
WCE: WS08_0654
WCT: WS74_0656
CLS: CXIVA_00050(Aes)
OVA: OBV_06920
BPB: bpr_IV024
BFI: CIY_00430
BHU: bhn_I0251
RIX: RO1_04110
RIM: ROI_00870
CCT: CC1_05780
BPRO: PMF13cell1_05279(mlhB)
CSO: CLS_33060
EHL: EHLA_3068
ERT: EUR_27150
ERA: ERE_06890
DSY: DSY1047
DHD: Dhaf_2129
BPRS: CK3_29550
FMA: FMG_1381
STED: SPTER_15950(aes)
MTV: RVBD_3084
MRA: MRA_3116(lipR)
MTUR: CFBS_3252(lipR)
MTO: MTCTRI2_3148(lipR)
MTD: UDA_3084(lipR)
MTN: ERDMAN_3375(lipR)
MTUE: J114_16500
MTUH: I917_21660
MTUL: TBHG_03014
MTUT: HKBT1_3239(lipR)
MTUU: HKBT2_3244(lipR)
MTQ: HKBS1_3250(lipR)
MBO: BQ2027_MB3111(lipR)
MBT: JTY_3104(lipR)
MBM: BCGMEX_3106(lipR)
MBX: BCGT_2934
MAF: MAF_30910(lipR)
MMIC: RN08_3396
MCE: MCAN_31101(lipR)
MCQ: BN44_60600(lipR)
MCV: BN43_60076(lipR)
MCX: BN42_41101(lipR)
MCZ: BN45_60072(lipR)
MPA: MAP_2197(lipK)
MAO: MAP4_1625
MAVI: RC58_08115
MAVU: RE97_08115
MIT: OCO_18580
MIA: OCU_18760
MUL: MUL_2832(lipU_1) MUL_3067
MJL: Mjls_3202
MMI: MMAR_3137
MLI: MULP_00675(lipU_1) MULP_02435
MSAK: MSAS_01440
MCOO: MCOO_01360
MBAI: MB901379_02486(lipR_2)
MSG: MSMEI_2935(lipR)
MGI: Mflv_4606
MDU: MDUV_32500(mbtJ)
MAUU: NCTC10437_01240(mlhB_1)
MMAG: MMAD_12720(mbtJ)
MPSC: MPSYJ_52210(mbtJ)
MARZ: MARA_26510(mbtJ)
MGAD: MGAD_26470(mbtJ)
MPOF: MPOR_17670(mbtJ)
MBOK: MBOE_24380
MAB: MAB_2076
MCHE: BB28_23770
MSTE: MSTE_00831 MSTE_01521(bah_1) MSTE_03925(bah_2)
MSAL: DSM43276_00393(lipF_1) DSM43276_00710(lipF_2)
MJD: JDM601_0907(lipU) JDM601_1606(mbtJ)
MTER: 4434518_00874(lipU_1) 4434518_03700(lipR) 4434518_03885(mlhB)
MHIB: MHIB_04060
CUR: cu1816
CVA: CVAR_0330
CTER: A606_01340
CPRE: Csp1_23990(mlhB)
NFR: ERS450000_00738(mlhB_1) ERS450000_05227(mlhB_4)
NAD: NCTC11293_04103(mlhB_3) NCTC11293_06010(mlhB_4)
RFA: A3L23_01126(lipF_2) A3L23_03142(lipR)
RHS: A3Q41_00187(lipR) A3Q41_02261(lipF_1)
RHU: A3Q40_02276(lipR)
RRT: 4535765_01378(mlhB_1) 4535765_04627(lip2_7)
RCR: NCTC10994_01421(mlhB_2) NCTC10994_02868(lip2_3)
GRU: GCWB2_00565(lipF1) GCWB2_12730(mlhB) GCWB2_21960(lipF2)
GOM: D7316_01086(mlhB) D7316_03543(lipF_1) D7316_03816(lipF_2) D7316_04097(lipF_3)
SCO: SCO3415(SCE9.22)
SBH: SBI_01199
SALU: DC74_221
SALL: SAZ_01570
STRE: GZL_01558
STRM: M444_06090
SRW: TUE45_02116(lipR)
SLX: SLAV_32710(mlhB)
MTS: MTES_1158
MLV: CVS47_01889(mlhB)
ART: Arth_1841
AAGI: NCTC2676_1_02465(mlhB_2)
NDK: I601_1653(mlhB_2)
PSIM: KR76_25940
NAL: B005_0480
STRR: EKD16_11705(mlhB)
SRO: Sros_3441
NCX: Nocox_19515(mlhB1) Nocox_21845(mlhB2) Nocox_24690(mlhB3)
TBI: Tbis_1867
MMAR: MODMU_4064
AMYY: YIM_18340(mlhB1) YIM_22075(mlhB2) YIM_32910(mlhB3)
PDX: Psed_0489
PSEE: FRP1_01730
PSEH: XF36_18485
ACTN: L083_1282
AFS: AFR_22850
PDO: PSDT_0042
ATM: ANT_01270
GPN: Pan110_36300(lipR)
PBOR: BSF38_01313(mlhB)
TPB: TPFB_0935
ACA: ACP_1899(est)
SUS: Acid_2102
FSC: FSU_0418
GAU: GAU_2134
CPI: Cpin_4628
SGN: SGRA_1209
HSW: Hsw_2878
FLM: MY04_0387
MFC: BRM9_1847
METM: MSMTP_1327
MEMA: MMAB1_0486(bah)
MBN: Mboo_0641
TAA: NMY3_03016(mlhB)
 » show all
Reference
1  [PMID:11157238]
  Authors
van der Vlugt-Bergmans CJ, van der Werf MJ
  Title
Genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from Rhodococcus erythropolis DCL14.
  Journal
Appl Environ Microbiol 67:733-41 (2001)
DOI:10.1128/AEM.67.2.733-741.2001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.83
IUBMB Enzyme Nomenclature: 3.1.1.83
ExPASy - ENZYME nomenclature database: 3.1.1.83
BRENDA, the Enzyme Database: 3.1.1.83

DBGET integrated database retrieval system