KEGG   ENZYME: 3.1.2.29Help
Entry
EC 3.1.2.29                 Enzyme                                 

Name
fluoroacetyl-CoA thioesterase
Class
Hydrolases;
Acting on ester bonds;
Thioester hydrolases
BRITE hierarchy
Sysname
fluoroacetyl-CoA hydrolase
Reaction(IUBMB)
fluoroacetyl-CoA + H2O = fluoroacetate + CoA [RN:R09643]
Reaction(KEGG)
Substrate
fluoroacetyl-CoA [CPD:C19775];
H2O [CPD:C00001]
Product
fluoroacetate [CPD:C06108];
CoA [CPD:C00010]
Comment
Fluoroacetate is extremely toxic. It reacts with CoA to form fluoroacetyl-CoA, which substitutes for acetyl CoA and reacts with EC 2.3.3.1 (citrate synthase) to produce fluorocitrate, a metabolite of which binds very tightly to EC 4.2.1.3 (aconitase) and halts the TCA cycle. This enzyme hydrolyses fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance on the organisms that produce it. It has been described in the poisonous plant Dichapetalum cymosum and the bacterium Streptomyces cattleya, both of which are fluoroacetate producers.
History
EC 3.1.2.29 created 2011
Orthology
K18700  fluoroacetyl-CoA thioesterase
Genes
DJI: CH75_12785
PSJ: PSJM300_07535
PSIL: PMA3_16455
HAZ: A9404_01960
BSAN: CHH28_03570
GBI: PG2T_09105
SLIM: SCL_2604
SVA: SVA_3777
RSM: CMR15_mp10105
RPJ: N234_00730 N234_11085
REH: H16_A0188(h16_A0188)
CGD: CR3_0095(flK)
BCEW: DM40_4005
BCT: GEM_3710
BUB: BW23_5832
BLAT: WK25_22535
BGU: KS03_3927
BPH: Bphy_3182
PPNO: DA70_14415
PPNM: LV28_16265
PPUL: RO07_10395
PSPU: NA29_06220
PAPI: SG18_10800
OFO: BRW83_0360(flK)
SLT: Slit_0397
GCA: Galf_1995
EBA: ebB192
AZA: AZKH_2894
TCL: Tchl_2129
BPRC: D521_1615
ABU: Abu_0882
ABT: ABED_0834
ATP: ATR_1085
APOC: APORC_1361
HBV: ABIV_1630
SUN: SUN_0938
GBM: Gbem_3982
GEB: GM18_2349
DEU: DBW_1408(flK)
DDS: Ddes_0080
DALK: DSCA_35490
SFU: Sfum_3560
BRA: BRADO6036
BBT: BBta_1742
BRS: S23_13410
AOL: S58_62090
BRAD: BF49_2989
RPB: RPB_1187
RPE: RPE_0936
RPT: Rpal_1367
OCA: OCAR_5925
VGO: GJW-30_1_00689(flK_1) GJW-30_1_01509(flK_2)
FIL: BN1229_v1_3097(flK)
FIY: BN1229_v1_2823(flK)
MCG: GL4_2070
RRU: Rru_A0734
RRF: F11_03765
MAG: amb1240
MAGX: XM1_1872
MAGN: WV31_17120
TMO: TMO_b0280
BEO: BEH_00935
OIH: OB2879
ANM: GFC28_426
AAMY: GFC30_1771
VPN: A21D_01987(flK)
PSYO: PB01_19090
ASOC: CB4_03661(flK)
AAC: Aaci_0813
AAD: TC41_0811
BTS: Btus_1242
ECAS: ECBG_01836
CTC: CTC_00206
CBK: CLL_A2218
CBN: CbC4_0165
CBT: CLH_2019
CBE: Cbei_2480
CBZ: Cbs_2480
CBEI: LF65_03049
CKL: CKL_0173
CKR: CKR_0142
CSQ: CSCA_4243
CTYK: CTK_C29310
AMT: Amet_0908
CTH: Cthe_2252
CCE: Ccel_0086
ESR: ES1_11380
ESU: EUS_05280
RCH: RUM_02020
FPR: FP2_32500
FPA: FPR_17620
BFI: CIY_15280
RIX: RO1_39090
RIM: ROI_28450
CCT: CC1_03810
CSO: CLS_11080
BPRL: CL2_26670
CPRO: CPRO_27490(flK)
EHL: EHLA_2441
ERT: EUR_19040
ERA: ERE_05620
DSY: DSY1906
DHD: Dhaf_3066
DRM: Dred_0128
PTH: PTH_1667
SGY: Sgly_2172
HMO: HM1_0673
ELM: ELI_3780
OVA: OBV_26430
TMR: Tmar_1224
SAY: TPY_1550
CTHM: CFE_0087
BPRM: CL3_08010
BPRS: CK3_00200
CHY: CHY_2386
ADG: Adeg_1847
ATE: Athe_2209
TACI: TDSAC_1644
VPR: Vpar_0746
MED: MELS_1131
PUF: UFO1_3267
PFT: JBW_00119
AIN: Acin_0216
PFAC: PFJ30894_00131(flK)
LPIL: LIP_0304
MSHG: MSG_02389
CGL: NCgl0442(Cgl0457)
CGB: cg0542
CGU: WA5_0442
CGT: cgR_0530
CGM: cgp_0542
CGJ: AR0_02835
CEF: CE1195
SCT: SCAT_4159(flK)
SRW: TUE45_00627(flK)
SRN: A4G23_04197(flK)
SRJ: SRO_6982
NCA: Noca_1708
NDK: I601_3968
PSIM: KR76_10220
TFU: Tfu_0552
STRR: EKD16_04330(flK)
SRO: Sros_8316
GOB: Gobs_1901
MMAR: MODMU_2289
AMYY: YIM_23690(flK)
PAUT: Pdca_29320
SAQ: Sare_3259
MIL: ML5_4054
ASE: ACPL_1908
ACTN: L083_2232
AFS: AFR_10985
ACTS: ACWT_1786
CAI: Caci_7731
SNA: Snas_3802
TBI: Tbis_2916
STI: Sthe_2574
DGE: Dgeo_2156
DFC: DFI_00680
TRA: Trad_1897
TTJ: TTHA0967
TAQ: TO73_0993
TBC: A0O31_00110(flK)
MRB: Mrub_1320
PLH: VT85_17250(flK)
GMR: GmarT_48070(flK)
SACI: Sinac_5066
PBOR: BSF38_02074(flK)
AGV: OJF2_54980(flK)
SUS: Acid_4405
TLI: Tlie_1650
BVU: BVU_1957
BOA: Bovatus_01252(flK)
PRU: PRU_1498
AFD: Alfi_2770
ASH: AL1_28540
BLQ: L21SP5_01377(flK)
MBAS: ALGA_0299
ASAC: ATHSA_0585
MOX: DAMO_1518
MARC: AR505_1499
ABI: Aboo_0696
MAC: MA_0038
HHB: Hhub_3479
NMO: Nmlp_1577
SMR: Smar_1507
DKA: DKAM_0685
TAG: Tagg_0130
IAG: Igag_1870
HBU: Hbut_1011
STO: STK_06730
SOL: Ssol_2310
SSOA: SULA_2340
SSOL: SULB_2341
SSOF: SULC_2338
AHO: Ahos_2122
STEP: IC006_0216
ASC: ASAC_0040
NEV: NTE_03122
TAA: NMY3_03720(flK)
NFN: NFRAN_1008(flK)
NCV: NCAV_0469
 » show all
Taxonomy
Reference
1
  Authors
Meyer, J.J.M., Grobbelaar, N., Vleggaar, R. and Louw, A.I.
  Title
Fluoroacetyl-coenzyme-A hydrolase-like activity in Dichapetalum cymosum.
  Journal
J Plant Physiol 139:369-372 (1992)
Reference
2  [PMID:16720268]
  Authors
Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, Leadlay PF, Spencer JB
  Title
The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A.
  Journal
Chem Biol 13:475-84 (2006)
DOI:10.1016/j.chembiol.2006.02.014
  Sequence
[sct:SCAT_4159]
Reference
3  [PMID:20430898]
  Authors
Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, Leadlay PF, Spencer JB, Blundell TL
  Title
Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK.
  Journal
J Biol Chem 285:22495-504 (2010)
DOI:10.1074/jbc.M110.107177
  Sequence
[sct:SCAT_4159]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.2.29
IUBMB Enzyme Nomenclature: 3.1.2.29
ExPASy - ENZYME nomenclature database: 3.1.2.29
BRENDA, the Enzyme Database: 3.1.2.29

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