KEGG   ENZYME: 3.1.2.29
Entry
EC 3.1.2.29                 Enzyme                                 
Name
fluoroacetyl-CoA thioesterase
Class
Hydrolases;
Acting on ester bonds;
Thioester hydrolases
Sysname
fluoroacetyl-CoA hydrolase
Reaction(IUBMB)
fluoroacetyl-CoA + H2O = fluoroacetate + CoA [RN:R09643]
Reaction(KEGG)
R09643
Substrate
fluoroacetyl-CoA [CPD:C19775];
H2O [CPD:C00001]
Product
fluoroacetate [CPD:C06108];
CoA [CPD:C00010]
Comment
Fluoroacetate is extremely toxic. It reacts with CoA to form fluoroacetyl-CoA, which substitutes for acetyl CoA and reacts with EC 2.3.3.1 (citrate synthase) to produce fluorocitrate, a metabolite of which binds very tightly to EC 4.2.1.3 (aconitase) and halts the TCA cycle. This enzyme hydrolyses fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance on the organisms that produce it. It has been described in the poisonous plant Dichapetalum cymosum and the bacterium Streptomyces cattleya, both of which are fluoroacetate producers.
History
EC 3.1.2.29 created 2011
Orthology
K18700  fluoroacetyl-CoA thioesterase
Genes
LAREHIV01_017850
DJICH75_12785
PSILPMA3_16455
PSJPSJM300_07535
HAZA9404_01960
BSANCHH28_03570
GBIPG2T_09105
SLIMSCL_2604
SVASVA_3777
RSMCMR15_mp10105
RPJN234_00730 N234_11085
REHH16_A0188(h16_A0188)
CNCCNE_1c01790 CNE_2c21370
CUHBJN34_00860
REUReut_A0155
CTIRALTA_A0131 RALTA_A1649
CGDCR3_0095(flK)
COXE0W60_11530 E0W60_19270
BCEWDM40_4005
BCTGEM_3710
BCEPAPZ15_32795
BPYRABD05_24835
BUBBW23_5832
BLATWK25_22535
BANNJFN94_27870 JFN94_27895
BGLbglu_2g19990
BGUKS03_3927
BYIBYI23_C011430 BYI23_C013520
BUEBRPE67_CCDS12970 BRPE67_CCDS15150
BURKDM992_22110
BPHBphy_3182
BPXBUPH_02668
PHSC2L64_26035
PMEGFNZ07_29015
PPKU875_21740
PPNODA70_14415
PPNMLV28_16265
PRBX636_12430
PPULRO07_10395
PSPUNA29_06220
PAPISG18_10800
PTXABW99_14960
PNRAT302_10130
PFIBPI93_001850
BUOBRPE64_DCDS08010 BRPE64_DCDS08700
AXONH44784_041571
ADTAPT56_11470
ACHRC2U31_08270
RHYRD110_03605 RD110_09030
RHFEUB48_15605
POSDT070_17495
POOF7R28_13090
VAPVapar_2079
ADKAlide2_2761
STHMIS481_02755
MASWAM586_01330
MASSCR152_02270
MANCIV454_08365
OFOBRW83_0360(flK)
NOKFAY22_19800
SHDSUTH_01618
SLTSlit_0397
GCAGalf_1995
FKUFGKAn22_18030
SEMEMIZ01_0295
SULFCAP31_07690
SLACSKTS_28660
URUDSM104443_00464 DSM104443_03423(flK)
UPLDSM104440_00366 DSM104440_00533
ARESIWH25_13905
EBAebB192
ABREpbN1_03570
APETToN1_39630
AZAAZKH_2894
AZIAzCIB_2835
AZDCDA09_15460
TMZTmz1t_2969
TCLTchl_2129
TAKTharo_1287
DDZDSYM_12640 DSYM_29360
BPRCD521_1615
BEBAEM42_06970
BEBABWI17_02820 BWI17_09885
ABUAbu_0882
ABTABED_0834
ABLA7H1H_0892
ASKEI285_02765
ATPATR_1085
ACIBACBT_1556
ACREACRYA_0878
ALANALANTH_1056
APOCAPORC_1361
AFCAFAEC_1427
ANTArnit_2115
AAQIAAQM_0910
ALPLPB137_09430
APAIAPAC_1672
HBVABIV_1630
SUNSUN_0938
GEBGM18_2349
GEFFO488_02330
GBMGbem_3982
DEUDBW_1408(flK)
DDSDdes_0080
DFIAXF13_14085
DEFCNY67_00435
DPRDespr_2396
DEOCAY53_06070
DOGHP555_13995
DBKDGMP_02880
DMLDmul_25410
DOVDSCO28_34590
DALKDSCA_35490
DLIdnl_33280
AFWAnae109_3233
DAODesac_0943 Desac_1007
DTIDesti_5521
SFUSfum_3560
MESMEJ066_00550
EMXFKV68_15750
BJUBJ6T_62860
BJPRN69_09060 RN69_30465
BRABRADO6036
BBTBBta_1742
BRSS23_13410
AOLS58_62090
BRADBF49_2989
BICLMTR13_33075
BROBRAD285_6006
BRKCWS35_36945
BOTCIT37_17920
BRQCIT40_04190 CIT40_11485
BGQX265_31600
BGZXH91_29995
BSYMCIT39_02545 CIT39_10625
BBETF8237_10000
BARHWN72_18705
BVZBRAD3257_1190
BELBE61_87130
RPBRPB_1187
RPDRPD_1291 RPD_1506
RPERPE_0936
RPTRpal_1367
RPXRpdx1_0928
OCAOCAR_5925
OCGOCA5_c20960
OCOOCA4_c20950
VGOGJW-30_1_00689(flK_1) GJW-30_1_01509(flK_2)
BOFFQV39_10170
METSDK389_23775
CDQBOQ54_14535
HDNHden_1941 Hden_2213
HDTHYPDE_28848 HYPDE_33998
HMCHYPMC_1299 HYPMC_2898
HNIW911_09815 W911_10150
FILBN1229_v1_3097(flK)
FIYBN1229_v1_2823(flK)
RHZRHPLAN_16120
MCGGL4_2070
METGHT051_08845
PSINCAK95_17200
RLIRLO149_c032200
LSALKBK07_10520
PAMNJCM7685_0856
TAWEI545_12225
FAQG5B39_00605
ATIAL072_27965
RRURru_A0734
RRFF11_03765
RPMRSPPHO_01411
MAGamb1240
MAGXXM1_1872
MAGNWV31_17120
TMOTMO_b0280
HTQFRZ44_00480 FRZ44_46870
HADHFRZ61_00460 FRZ61_44820
BIFN288_11505
BHKB4U37_11730
BEOBEH_00935
BONA361_12110
BKOCKF48_14845
CFIRNAF01_10805
OIHOB2879
GMCGY4MC1_1501
GTHGeoth_1593
PTLAOT13_14875
PTBDER53_14735
ANMGFC28_426
AAMYGFC30_1771
ANLGFC29_1198
ANDGRQ40_10595
ACAIISX45_08220
LMACI6G82_21940
VIGBKP57_13050
VILCFK37_07210
VPNA21D_01987(flK)
VPTKBP50_02885
FPNABE65_003860
FARABE41_003910
PSYOPB01_19090
POFGS400_11790
BVQFHE72_02650
BVJI5776_15080
BOUI5818_18770
EXMU719_01990
EXUESP131_04435
GMONCTC11323_00833
GEQCG018_06535
GSAFOC50_08585
GHANCTC10459_00822
BBEBBR47_49960
BFMBP422_20745
BAGRBA6348_06500
BCHSJNE38_27070
PLVERIC2_c10050
ANXACH33_14490
ASOCCB4_03661(flK)
ATHEK3F53_04410
AACAaci_0813
AADTC41_0811
BTSBtus_1242
KYRCVV65_09900
SPSYAZE41_14030
PANCE2636_08205
LFBC1X05_03535
TVUAB849_001900
KPULGXN76_04880
KEBGXN75_05620
PABSJIR001_07590
LACTD7I46_06185
LACKFLP15_11345
ECASECBG_01836
EGAAL523_10305
ESSATZ33_04285
EGVEGCR1_03920
EAVEH197_14250
ESGEsVE80_10360
VTEBHY08_06140
VACE4Z98_04990
VAOFA707_05595
VAHG7081_05725
VCPH9L18_07860 H9L18_10905
VHYG7082_12730
AURHMPREF9243_0474
AUNAWM73_07155
ASANAWM72_03440
ACGAWM71_04335
ABAECL176_07340
CMLBN424_1548
CDJBFC22_07745
JEHEJN90_00915
CTCCTC_00206
CTETBN906_00196
CNONT01CX_1041
CBKCLL_A2218
CBNCbC4_0165
CBTCLH_2019
CBECbei_2480
CBZCbs_2480
CBEILF65_03049
CKLCKL_0173
CKRCKR_0142
CLJCLJU_c39350
CLSCXIVA_00190
CLBClo1100_0082
CSBCLSA_c24070
CAHCAETHG_1780
CSQCSCA_4243
CACECACET_c03610
CCKCcar_20070
CBUTATN24_06735
CTYKCTK_C29310
CEUA7L45_08135 A7L45_09900
CTAEBGI42_08765
CFMBJL90_07480
CARGRSJ17_03270
CDRKB9W14_23885
CDYF3K33_12675
CFERD4Z93_02450 D4Z93_12415
CLOSDMR38_19620
AMTAmet_0908
GFEGferi_16845 Gferi_23430
CALEFDN13_08660
CRSFQB35_01210 FQB35_04855
CTHCthe_2252
CTXClo1313_2925
CCLClocl_0128
RBPB6259_09800
CCECcel_0086
RHEREHE19_000630
ESRES1_11380
ESUEUS_05280
FPLAA4U99_08745
RCHRUM_02020
RUJE5Z56_03765
FPRFP2_32500
FPAFPR_17620
FPRACG447_08435
FDUGXM22_00515
CAPREQM14_00155
OVAOBV_26430
OBJEIO64_01010
CAMLH6X83_13940
CLFGJQ69_09245
SMANC12CBH8_15080
CFEMHCR03_11875
ACOLK5I23_15085
BFICIY_15280
RHORHOM_03470
RIXRO1_39090
RIMROI_28450
CCTCC1_03810
CSOCLS_11080
BPRLCL2_26670
ARFAR1Y2_0898 AR1Y2_3117
ACACEYQ97_12670
CPROCPRO_27490(flK)
LUAD4A81_09455
EHLEHLA_2441
PXVFXF36_14260
ERTEUR_19040
ERAERE_05620
EACEAL2_c17940
DSYDSY1906
DHDDhaf_3066
DDHDesde_2535
DDLDesdi_2140
DMTDESME_06180
SGYSgly_2172
DORDesor_3086 Desor_3428
DAIDesaci_3166
DMIDesmer_1794 Desmer_2692
PTHPTH_1667
DRMDred_0128
DFGB0537_08630
TFRBR63_14515
FWADCMF_21655
AACXDEACI_1716
TJRTherJR_0207
DGIDesgi_0161
HMOHM1_0673
HCVFTV88_2657
ELMELI_3780
EMTCPZ25_009140
ELIMB2M23_07560
AWOAwo_c29810
ALKAJ0B03_06080
TMRTmar_1224
THEFE1B22_09745
THEPDYI95_006095
SAYTPY_1550
SAPSulac_1752
STHRBXT84_12380 BXT84_14435
CTHMCFE_0087
IBUIB211_01561c
MDVC5Q96_05595
EUUADJ67_02370
BPRMCL3_08010
BPRSCK3_00200
CHYCHY_2386
ADGAdeg_1847
GEKkuro4_15330
ATEAthe_2209
COBCOB47_1987
CHDCalhy_0554
COWCalow_1895
CKICalkr_0369
CLCCalla_1990
CKNCalkro_0432
CDIACaldiYA01_03850
TNRThena_1675
TACITDSAC_1644
MASMahau_0619
NTHNther_1839
KMEH0A61_00495(flK_1) H0A61_00779(flK_2)
CAZOG3A45_12725
SEDZJYG23_12600
VPRVpar_0746
VATB7L28_03555
VRM44547418_00755
VDNNCTC11831_01202
VNKVEIT17_10830
MEDMELS_1131
MHWACT01_09310
MEGDKB62_02255
MMAXLCQ47_03590
DHODia5BBH33_01230 Dia5BBH33_14030
SSGSelsp_0905
SRISELR_23920
SELEADJ74_06125
SELOAXE86_03410
SELTBCS37_09345
MFUNGXM21_07210
PUFUFO1_3267
PFTJBW_00119
STEDSPTER_11360
AFNAcfer_0121
AINAcin_0216
PFACPFJ30894_00131(flK)
FROAALO17_22840
AARGAargi30884_13650
ABSIA9CBEGH2_14480
CIUG4D55_16825
LCGL3BBH23_05250
ERBA4V01_19550
LPILLIP_0304
MSHGMSG_02389
MTYMTOK_51950
MHEVMHEL_08280
CGLCgl0457(Cgl0457)
CGBcg0542
CGUWA5_0442
CGTcgR_0530
CGSC624_02660
CGGC629_02660
CGMcgp_0542
CGJAR0_02835
CGQCGLAR1_02700
CGXSB89_02515
CEFCE1195
CCNH924_05340
CFNCFAL_00070
CVTB843_00440
CDXCDES_05480
CCJZccrud_02395
CCHOCCHOA_08225
CSURN24_0599
BFVC628_02695
NNONONO_c39650
NAHF5544_24280
ROAPd630_LPD04762
SSXSACTE_6536
SCTSCAT_4159(flK)
SCYSCATT_41470
SRWTUE45_00627(flK)
SRNA4G23_04197(flK)
STRTA8713_30605
SCADDN051_09745
SVUB1H20_22575
SPUNBFF78_02590
SRJSRO_6982
SDXC4B68_02600
SGFHEP81_07316
SSEOD0Z67_28305
SBYH7H31_02540
SCYGS1361_36410(flK)
SFBCP974_22630
ICAIntca_1420
TEHGKE56_11975
TFLRPIT_05230
TESBW730_04770
RAINRai3103_09470
NCANoca_1708
NDKI601_3968
NOYEXE57_05375 EXE57_11570
NOIFCL41_11650
NOOFE634_08030
NSNEXE58_14035
NANOG5V58_14865
NMESH9L09_15590
NAROCFH99_09595
PSIMKR76_10220
AERAERYTH_06475
AEZC3E78_04630
AEBC6I20_10615
AEFGEV26_04455
MUZH4N58_05765
TFUTfu_0552
THAONI17_023555
TALXFOF52_20650
NGVCDO52_08130
STRREKD16_04330(flK)
SROSros_8316
NOABKM31_52475
NOWGBF35_36750 GBF35_43780
NCXNocox_36020(flK)
NGNLCN96_48860
TBITbis_2916
GOBGobs_1901
MMARMODMU_2289
AMYYYIM_23690(flK)
PAUTPdca_29320
STPStrop_3033
SAQSare_3259
MAUMicau_4249
MILML5_4054
MICBMicB006_3243
MTUACSH63_03385
MICHFJK98_16205 FJK98_22760
MTEMGCE86_15590
MCABHXZ27_21620
MSAGGCM10017556_04750
VMAVAB18032_25695
MCRAID554_24975
ASEACPL_1908
AMSAMIS_16610
ACTNL083_2232
AFSAFR_10985
ACTSACWT_1786
AIHAiant_62510
PLKCIK06_19375
PLABC6361_24920
PLATC6W10_33280
PFLAPflav_049160
PSUUPsuf_006970
VERHUT12_22355
ATLAthai_33280
ASERAsera_41330
PRYPrubr_01830
CATICS0771_71570
CCAZCOUCH_28775
CAICaci_7731
SNASnas_3802
EKEEK0264_03140
ABAIIMCC26256_11306
BSOLFSW04_11730
AYMYM304_10880
ATQGH723_13910
AQZKSP35_11065
OLOADJ70_01680
EUZDVS28_a1098
LENLEP3755_11180
STISthe_2574
ABAOBEQ56_06210
KBSEPA93_42805
TBHTbon_11180
DGEDgeo_2156
DDRDeide_20170
DMRDeima_1127
DPTDeipr_0507
DGODGo_CA2498
DPDDeipe_0813
DSWQR90_08105
DCHSY84_11230
DABAUC44_02500
DPUSU48_12110
DWUDVJ83_10915
DFCDFI_00680
DEINDAAJ005_08735
DGADEGR_04440
DRDLMT64_00980
TRATrad_1897
TTJTTHA0967(TTHA0967)
TTSThthe16_0965
TTLTtJL18_1083
TSCTSC_c10540
THCTCCBUS3UF1_12340
TOSTheos_0981
TAQTO73_0993
TPARAV541_05860
TBCA0O31_00110(flK)
TANTKNN15_10330
MRBMrub_1320
MREK649_06255
MSVMesil_2323
MTAIMtai_v1c16560
MHDMarky_1275
PLHVT85_17250(flK)
GMRGmarT_48070(flK)
GIMF1728_21225
GPNPan110_47940(flK)
MRIMal4_19030(flK)
SACISinac_5066
PBORBSF38_02074(flK)
AGVOJF2_54980(flK)
TPITREPR_2668
TRMJO41_06005 JO41_10645
TRZGWP43_09580 GWP43_12995
OCKEXM22_13855
SCCSpico_1568
BHCJFL75_20385
SUSAcid_4405
PFERIRI77_21240
CTMCabther_A0738
CVLJ8C06_06860
CHLOJ8C02_03575
FNTHMPREF0405_00883
FUSHMPREF0409_00541
FNEFSDG_00644
FHWRN87_07655
FPDCTM68_10105
FMOC4N19_05970
FGOC4N16_04335
FNFBSQ88_02905
FPEIC4N17_03330
FCII6I83_07270
IPOIlyop_1341
SNSVC03_04305
ACOAmico_0029
TLITlie_1650
AMOAnamo_0226
BOABovatus_01252(flK)
BVUBVU_1957
BSABacsa_2604
BDOEL88_17490
BDHGV66_10070
PAHPoras_0003
PCRENCTC12858_01403
PPNPalpr_0808
OSPOdosp_1654
BUYD8S85_03085
BVIRI6J59_10555
PRUPRU_1498
PROHMPREF0669_00672
PEOAS203_10850
AFDAlfi_2770
ASHAL1_28540
AOKA3BBH6_00630
ACOUA5CBH24_20900
ADAA5CPEGH6_02350
RBCBN938_0733
TTZFHG85_07035
BLQL21SP5_01377(flK)
BACCBRDCF_p1045
ASXCDL62_16080
MBASALGA_0299
FEIK9M53_12765
HYDPK28_13680
HYEAM218_02765
HYHD3Y59_00540
HYJFHG12_14030
HQIH9L05_12380
HRSHER32_05410
MTTFtrac_0057
ASACATHSA_0585
ALAMRT761_01999(flK)
MOXDAMO_1518
MACMA_0038
MVCMSVAZ_2211
MEKMSKOL_2207
HHBHhub_3479
HALXM0R89_10055
HAXZM0R88_10240
NMONmlp_1577
HLMDV707_16800
TARTALC_00314
MAXMMALV_02850
MEARMpt1_c10750
MARCAR505_1499
ABIAboo_0696
ACFAciM339_0365
SMRSmar_1507
SHCShell_0937
DKADKAM_0685
DFDDesfe_0824
DMUDesmu_0580
TAGTagg_0130
IAGIgag_1870
THGTCELL_0079
HBUHbut_1011
STOSTK_06730
SOHD1869_03240
SOLSsol_2310
SSOASULA_2340
SSOLSULB_2341
SSOFSULC_2338
AHOAhos_2122
AMANB6F84_01855
ABRIDFR85_05905
ASULDFR86_01840
AAMBD1866_05610
ACIHHS5_09370
SAZOD1868_03930
CSTYKN1_23330
STEPIC006_0216
THBN186_08770
TCBTCARB_0832
THFMA03_03575
ASCASAC_0040
FFOFFONT_0779
NGANgar_c00920
NVNNVIE_024250
NEVNTE_03122
TAANMY3_03720(flK)
NFNNFRAN_1008(flK)
NCVNCAV_0469
KCRKcr_0215 Kcr_0526
 » show all
Reference
1
  Authors
Meyer, J.J.M., Grobbelaar, N., Vleggaar, R. and Louw, A.I.
  Title
Fluoroacetyl-coenzyme-A hydrolase-like activity in Dichapetalum cymosum.
  Journal
J Plant Physiol 139:369-372 (1992)
Reference
2  [PMID:16720268]
  Authors
Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, Leadlay PF, Spencer JB
  Title
The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A.
  Journal
Chem Biol 13:475-84 (2006)
DOI:10.1016/j.chembiol.2006.02.014
  Sequence
[sct:SCAT_4159]
Reference
3  [PMID:20430898]
  Authors
Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, Leadlay PF, Spencer JB, Blundell TL
  Title
Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK.
  Journal
J Biol Chem 285:22495-504 (2010)
DOI:10.1074/jbc.M110.107177
  Sequence
[sct:SCAT_4159]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.2.29
IUBMB Enzyme Nomenclature: 3.1.2.29
ExPASy - ENZYME nomenclature database: 3.1.2.29
BRENDA, the Enzyme Database: 3.1.2.29

DBGET integrated database retrieval system