KEGG   ENZYME: 3.1.21.9Help
Entry
EC 3.1.21.9                 Enzyme                                 

Name
T4 deoxyribonuclease IV;
T4 endonuclease IV;
EndoIV (ambiguous);
denB (gene name)
Class
Hydrolases;
Acting on ester bonds;
Endodeoxyribonucleases producing 5'-phosphomonoesters
BRITE hierarchy
Reaction(IUBMB)
Endonucleolytic cleavage of the 5' phosphodiester bond of deoxycytidine in single-stranded DNA.
Comment
This phage T4 enzyme is involved in degradation of host DNA. The enzyme does not cleave double-stranded DNA or native T4 DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
History
EC 3.1.21.9 created 2014
Orthology
K20842  T4 deoxyribonuclease IV
Reference
1  [PMID:4900512]
  Authors
Sadowski PD, Hurwitz J
  Title
Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli.
  Journal
J Biol Chem 244:6192-8 (1969)
Reference
2  [PMID:11946172]
  Authors
Ling V
  Title
Partial digestion of 32P-fd DNA with T(4) endonuclease IV.
  Journal
FEBS Lett 19:50-54 (1971)
DOI:10.1016/0014-5793(71)80602-6
Reference
3  [PMID:4550601]
  Authors
Sadowski PD, Bakyta I
  Title
T4 endonuclease IV. Improved purification procedure and resolution from T4 endonuclease 3.
  Journal
J Biol Chem 247:405-12 (1972)
Reference
4  [PMID:782881]
  Authors
Bernardi A, Maat J, de Waard A, Bernardi G
  Title
Preparation and specificity of endonuclease IV induced by bacteriophage T4.
  Journal
Eur J Biochem 66:175-9 (1976)
DOI:10.1111/j.1432-1033.1976.tb10437.x
Reference
5  [PMID:16971463]
  Authors
Hirano N, Ohshima H, Takahashi H
  Title
Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis.
  Journal
Nucleic Acids Res 34:4743-51 (2006)
DOI:10.1093/nar/gkl553
  Sequence
[vg:1258726]
Reference
6  [PMID:17940096]
  Authors
Ohshima H, Hirano N, Takahashi H
  Title
A hexanucleotide sequence (dC1-dC6 tract) restricts the dC-specific cleavage of single-stranded DNA by endonuclease IV of bacteriophage T4.
  Journal
Nucleic Acids Res 35:6681-9 (2007)
DOI:10.1093/nar/gkm721
  Sequence
[vg:1258726]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.21.9
IUBMB Enzyme Nomenclature: 3.1.21.9
ExPASy - ENZYME nomenclature database: 3.1.21.9
BRENDA, the Enzyme Database: 3.1.21.9

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