Acting on ester bonds;
Endoribonucleases producing 5'-phosphomonoesters
Endonucleolytic cleavage to a 5'-phosphomonoester
This is an endoribonuclease that cleaves double-stranded RNA molecules . The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site . Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage . RNase III is involved in both rRNA processing and mRNA processing and decay.