Hydrolases;
Acting on ester bonds;
Phosphoric-monoester hydrolases
BRITE hierarchy

NADPH 2'-phosphohydrolase

(1) NADPH + H2O = NADH + phosphate [RN:R12587];
(2) NADP+ + H2O = NAD+ + phosphate [RN:R00118]

R00118 R12587
Reaction

NADPH [CPD:C00005];
H2O [CPD:C00001];
NADP+ [CPD:C00006]

The mammalian mitochondrial enzyme is a rhythmically expressed protein that regulates metabolism under the control of circadian clock. It has a slight preference for NADPH over NADP+. The archaeal enzyme, identified in Methanocaldococcus jannaschii, is bifunctional acting as NAD+ kinase (EC 2.7.1.23) and NADP+ phosphatase with a slight preference for NADP+ over NADPH.

EC 3.1.3.108 created 2020

1  [PMID:18391451]

Kawai S, Murata K

Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H).

Biosci Biotechnol Biochem 72:919-30 (2008)
DOI:10.1271/bbb.70738

[mja:MJ_0109]

2  [PMID:29860338]

Abshire ET, Chasseur J, Bohn JA, Del Rizzo PA, Freddolino PL, Goldstrohm AC, Trievel RC

The structure of human Nocturnin reveals a conserved ribonuclease domain that represses target transcript translation and abundance in cells.

Nucleic Acids Res 46:6257-6270 (2018)
DOI:10.1093/nar/gky412

[hsa:25819]

3  [PMID:30389976]

Estrella MA, Du J, Korennykh A

Crystal Structure of Human Nocturnin Catalytic Domain.

Sci Rep 8:16294 (2018)
DOI:10.1038/s41598-018-34615-0

[hsa:25819]

4  [PMID:31147539]

Estrella MA, Du J, Chen L, Rath S, Prangley E, Chitrakar A, Aoki T, Schedl P, Rabinowitz J, Korennykh A

The metabolites NADP(+) and NADPH are the targets of the circadian protein Nocturnin (Curled).

Nat Commun 10:2367 (2019)
DOI:10.1038/s41467-019-10125-z

[hsa:25819]