KEGG   ENZYME: 3.1.4.50
Entry
EC 3.1.4.50                 Enzyme                                 
Name
glycosylphosphatidylinositol phospholipase D;
GPI-PLD;
glycoprotein phospholipase D;
phosphatidylinositol phospholipase D;
phosphatidylinositol-specific phospholipase D
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
Sysname
glycoprotein-phosphatidylinositol phosphatidohydrolase
Reaction(IUBMB)
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate [RN:R06623 R07398]
Reaction(KEGG)
R06623(G) R07398;
(other) R04149
Substrate
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248];
H2O [CPD:C00001]
Product
6-(alpha-D-glucosaminyl)-1D-myo-inositol [CPD:C15658];
3-sn-phosphatidate [CPD:C00416]
Comment
This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors, but does so by hydrolysis, whereas glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) does so by elimination. It acts on plasma membranes only after solubilization of the substrate with detergents or solvents, but it may act on intracellular membranes.
History
EC 3.1.4.50 created 1990, modified 2002
Pathway
ec00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
ec01100  Metabolic pathways
Orthology
K01127  glycosylphosphatidylinositol phospholipase D
Genes
HSA: 2822(GPLD1)
PTR: 462473(GPLD1)
PPS: 100973223(GPLD1)
GGO: 101146393(GPLD1)
PON: 100455517(GPLD1)
PPYG: 129039157(GPLD1)
NLE: 100598329(GPLD1)
HMH: 116459945(GPLD1)
SSYN: 129472852(GPLD1)
MCC: 707782(GPLD1)
MCF: 102125729(GPLD1)
MTHB: 126952975
MNI: 105482539(GPLD1)
CSAB: 103222053(GPLD1)
CATY: 105572618(GPLD1)
PANU: 101001857(GPLD1)
TGE: 112622303(GPLD1)
MLEU: 105552512(GPLD1)
RRO: 104663891(GPLD1)
RBB: 108533667(GPLD1)
TFN: 117086801(GPLD1)
PTEH: 111541103(GPLD1)
CANG: 105526706(GPLD1)
CJC: 100385911(GPLD1)
SBQ: 101035773(GPLD1)
CIMI: 108287071(GPLD1)
ANAN: 105721463(GPLD1)
CSYR: 103251843(GPLD1)
MMUR: 105865209(GPLD1)
LCAT: 123638333(GPLD1)
PCOQ: 105805640(GPLD1)
OGA: 100943003(GPLD1)
MMU: 14756(Gpld1)
MCAL: 110307663(Gpld1)
MPAH: 110333811(Gpld1)
RNO: 291132(Gpld1)
MCOC: 116081596(Gpld1)
ANU: 117713545(Gpld1)
MUN: 110551065(Gpld1)
CGE: 100759513(Gpld1)
MAUA: 101825977(Gpld1)
PROB: 127229384(Gpld1)
PLEU: 114700916(Gpld1)
MORG: 121434002(Gpld1)
MFOT: 126501644
AAMP: 119817603(Gpld1)
NGI: 103745185(Gpld1)
HGL: 101724062(Gpld1)
CPOC: 100734898(Gpld1)
CCAN: 109688371(Gpld1)
DORD: 106000038(Gpld1)
DSP: 122111059(Gpld1)
PLOP: 125352966(Gpld1)
NCAR: 124988281
MMMA: 107149294(Gpld1)
OCU: 100351916
OPI: 101523140(GPLD1)
TUP: 102501907(GPLD1)
GVR: 103596846(GPLD1)
CFA: 478735(GPLD1)
CLUD: 112657196(GPLD1)
VVP: 112920498(GPLD1)
VLG: 121499732(GPLD1)
NPO: 129508122(GPLD1)
AML: 100484842(GPLD1)
UMR: 103665176(GPLD1)
UAH: 113264336(GPLD1)
UAR: 123794261(GPLD1)
ELK: 111138612
LLV: 125101848
MPUF: 101688496(GPLD1)
MNP: 132017643(GPLD1)
MLK: 131834816(GPLD1)
NVS: 122913238(GPLD1)
ORO: 101370230(GPLD1)
EJU: 114212585(GPLD1)
ZCA: 113926556(GPLD1)
MLX: 118027226(GPLD1)
NSU: 110586839(GPLD1)
LWW: 102732488(GPLD1)
FCA: 101088486(GPLD1)
PYU: 121020624(GPLD1)
PCOO: 112853571(GPLD1)
PBG: 122489168(GPLD1)
PVIV: 125165239(GPLD1)
LRUF: 124528799
PTG: 102964289(GPLD1)
PPAD: 109252710(GPLD1)
PUC: 125939406
AJU: 106968449
HHV: 120219837(GPLD1)
BTA: 287025(GPLD1)
BOM: 102286788(GPLD1)
BIU: 109577315(GPLD1)
BBUB: 102389512(GPLD1)
BBIS: 104991130(GPLD1)
CHX: 102169718(GPLD1)
OAS: 101119000(GPLD1)
BTAX: 128056464(GPLD1)
ODA: 120870310(GPLD1)
CCAD: 122429730(GPLD1)
MBEZ: 129543195(GPLD1)
SSC: 100157497(GPLD1)
CFR: 102518271(GPLD1)
CBAI: 105061677(GPLD1)
CDK: 105089129(GPLD1)
VPC: 102538547(GPLD1)
BACU: 103003582(GPLD1)
BMUS: 118903415(GPLD1)
LVE: 103088629(GPLD1)
OOR: 101288738(GPLD1)
DLE: 111168561(GPLD1)
PCAD: 102975868(GPLD1)
PSIU: 116761576(GPLD1)
NASI: 112404172(GPLD1)
ECB: 100067517(GPLD1)
EPZ: 103546694(GPLD1)
EAI: 106822357(GPLD1)
MYB: 102260484(GPLD1)
MYD: 102753404(GPLD1)
MMYO: 118672726(GPLD1)
MDT: 132220742(GPLD1)
PKL: 118727102(GPLD1)
EFUS: 103291298(GPLD1)
MNA: 107539689(GPLD1)
DRO: 112313946(GPLD1)
SHON: 118990658(GPLD1)
AJM: 119054900(GPLD1)
PDIC: 114489512(GPLD1)
PHAS: 123833507(GPLD1)
MMF: 118637507(GPLD1)
PPAM: 129063916(GPLD1)
HAI: 109396397(GPLD1)
RFQ: 117015438(GPLD1)
PALE: 102896882(GPLD1)
PGIG: 120607804(GPLD1)
PVP: 105306900(GPLD1)
RAY: 107508879(GPLD1)
MJV: 108399074(GPLD1)
TOD: 119230328 119251689(GPLD1)
SARA: 101538377(GPLD1)
SETR: 125995999(GPLD1)
LAV: 100677378(GPLD1)
TMU: 101344243
ETF: 101648291(GPLD1)
DNM: 101430948(GPLD1)
MDO: 100024296(GPLD1)
GAS: 123240054(GPLD1)
SHR: 100931811(GPLD1)
AFZ: 127544109
PCW: 110208720(GPLD1)
TVP: 118850498(GPLD1)
OAA: 100092985(GPLD1)
GGA: 420819(GPLD1)
PCOC: 116239798(GPLD1)
MGP: 100546397(GPLD1)
CJO: 107309738(GPLD1)
TPAI: 128087940(GPLD1)
LMUT: 125690340(GPLD1)
NMEL: 110394624(GPLD1)
APLA: 101792700(GPLD1)
ACYG: 106038351(GPLD1)
CATA: 118249547(GPLD1)
AFUL: 116485918(GPLD1)
TGU: 100230775(GPLD1)
LSR: 110483464(GPLD1)
SCAN: 103819395(GPLD1)
PMOA: 120503505(GPLD1)
OTC: 121347820(GPLD1)
PRUF: 121357795(GPLD1)
GFR: 102032214(GPLD1)
FAB: 101816045(GPLD1)
OMA: 130249258(GPLD1)
PHI: 102114237(GPLD1)
PMAJ: 107200258(GPLD1)
CCAE: 111923724(GPLD1)
CCW: 104691444(GPLD1)
CBRC: 103613169(GPLD1)
ETL: 114069829(GPLD1)
ZAB: 102066942(GPLD1)
ZLE: 135444053(GPLD1)
ACHL: 103807301(GPLD1)
SVG: 106854115(GPLD1)
MMEA: 130579711(GPLD1)
HRT: 120750084(GPLD1)
SATI: 136364147(GPLD1)
FPG: 101912228(GPLD1)
FCH: 102052413(GPLD1)
CCRI: 104167493(GPLD1)
NNT: 104402944
SHAB: 115605387(GPLD1)
ACUN: 113476037(GPLD1)
TALA: 104368490(GPLD1)
ACHC: 115353059(GPLD1)
HALD: 104313504(GPLD1)
HLE: 104829910(GPLD1)
AGEN: 126048542
GCL: 127013027
CSTI: 104554877(GPLD1)
LDI: 104350256(GPLD1)
MNB: 103771284(GPLD1)
DPUB: 104302368(GPLD1)
AVIT: 104267477(GPLD1)
BRHI: 104495115(GPLD1)
EGZ: 104125477(GPLD1)
NNI: 104021793(GPLD1)
PCRI: 104029539(GPLD1)
PCAO: 104041515(GPLD1)
PADL: 103919061(GPLD1)
AFOR: 103903819(GPLD1)
FGA: 104078730(GPLD1)
GSTE: 104254087(GPLD1)
CLV: 102090900(GPLD1)
MUI: 104543338(GPLD1)
PGUU: 104457898(GPLD1)
PLET: 104618061(GPLD1)
CMAC: 104478271(GPLD1)
CUCA: 104062174(GPLD1)
TEO: 104383487(GPLD1)
BREG: 104642207(GPLD1)
OHA: 104335531(GPLD1)
ACAR: 104521825(GPLD1)
CPEA: 104394880(GPLD1)
CVF: 104288417(GPLD1)
RTD: 128905130(GPLD1)
AAM: 106483272(GPLD1)
AROW: 112972795(GPLD1)
NPD: 112958299 112958300
TGT: 104564647(GPLD1)
DNE: 112992496(GPLD1)
SCAM: 104153090(GPLD1)
ASN: 102375814(GPLD1)
AMJ: 102574726(GPLD1)
CPOO: 109316301(GPLD1)
GGN: 109302143(GPLD1)
PSS: 102448848(GPLD1)
CMY: 102944962(GPLD1)
CCAY: 125632201(GPLD1)
DCC: 119852264(GPLD1)
CPIC: 101945760(GPLD1)
TST: 117873259(GPLD1)
CABI: 116836102(GPLD1)
MRV: 120396443(GPLD1)
ACS: 100567850(gpld1)
ASAO: 132774639(GPLD1)
PVT: 110072878(GPLD1)
SUND: 121927761(GPLD1)
PBI: 103063314(GPLD1)
PMUR: 107289603(GPLD1)
CTIG: 120300001(GPLD1)
PGUT: 117672545(GPLD1)
APRI: 131195166(GPLD1)
PTEX: 113435583(GPLD1)
NSS: 113413590(GPLD1)
VKO: 123021649(GPLD1)
PMUA: 114600839(GPLD1)
PRAF: 128417707(GPLD1)
ZVI: 118090549(GPLD1)
HCG: 128323558(GPLD1)
GJA: 107113400(GPLD1)
STOW: 125439160(GPLD1)
EMC: 129333212(GPLD1)
XLA: 108718535
XTR: 116411679(gpld1)
NPR: 108792898(GPLD1)
RTEM: 120940771(GPLD1)
BBUF: 121001703(GPLD1)
BGAR: 122939268(GPLD1)
MUO: 115456470(GPLD1)
GSH: 117355635(GPLD1)
LCM: 102345190(GPLD1)
BFO: 118405295
BBEL: 109466135
SPU: 115926364 590298
LPIC: 129254944
APLC: 110975439
AJC: 117117987
SKO: 102801774
EAF: 111713084
PCAN: 112573356
BGT: 106061229
GAE: 121374135
HRF: 124113065
HRJ: 124288674
CRG: 105347431 105347449 105347450
CANU: 128165758 128165906 128166993
CVN: 111107846 111118101 111118284 111118285 111118286 111119154 111119995 111120355 111120568 111121275
OED: 125668748 125668749 125669462
MYI: 110456981
PMAX: 117326416 117326417
RPHI: 132760121
DPOL: 127843817
MAEA: 128214764
LJP: 135470662
LAK: 106165615
NVE: 5525170
EPA: 110241955
ATEN: 116291696
ADF: 107355065
PDAM: 113678713
SPIS: 111330701
DGT: 114523470
XEN: 124446939
HSY: 130636402
RES: 135684240
SCE: YJL132W
SPAO: SPAR_J00780
KLA: KLLA0_E15401g
KMX: KLMA_60039
LTH: KLTH0F05830g
ZRO: ZYRO0D03256g
CGR: 2890803(GVI51_L05599)
NCS: NCAS_0A09790(NCAS0A09790)
NDI: NDAI_0G06190(NDAI0G06190)
TBL: TBLA_0D05660(TBLA0D05660)
TGB: HG536_0F01060
TDL: TDEL_0H01030(TDEL0H01030)
KNG: KNAG_0C02670(KNAG0C02670)
ZMK: HG535_0C00920
PPA: PAS_chr4_0595
PKZ: C5L36_0C01010
BNN: FOA43_004168
OPA: HPODL_01688
YLI: 2906823(YALI2_C00450g)
SLB: AWJ20_2030
TML: GSTUM_00002772001
RIE: OCT59_017344
SRE: PTSG_02129
DDI: DDB_G0276919(pldG)
DPP: DICPUDRAFT_96554
DFA: DFA_04808(pldG)
ACAN: ACA1_147710
 » show all
Reference
1  [PMID:3422494]
  Authors
Low MG, Prasad AR
  Title
A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma.
  Journal
Proc Natl Acad Sci U S A 85:980-4 (1988)
DOI:10.1073/pnas.85.4.980
Reference
2  [PMID:3028377]
  Authors
Malik AS, Low MG.
  Title
Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases.
  Journal
Biochem J 240:519-27 (1986)
DOI:10.1042/bj2400519
Reference
3  [PMID:7961859]
  Authors
Li JY, Hollfelder K, Huang KS, Low MG.
  Title
Structural features of GPI-specific phospholipase D revealed by proteolytic fragmentation and Ca2+ binding studies.
  Journal
J Biol Chem 269:28963-71 (1994)
Reference
4  [PMID:11254757]
  Authors
Deeg MA, Bierman EL, Cheung MC.
  Title
GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.
  Journal
J Lipid Res 42:442-51 (2001)
Other DBs
ExplorEnz - The Enzyme Database: 3.1.4.50
IUBMB Enzyme Nomenclature: 3.1.4.50
ExPASy - ENZYME nomenclature database: 3.1.4.50
BRENDA, the Enzyme Database: 3.1.4.50
CAS: 113756-14-2

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (8)   
   KEGG COMPOUND (6)   
   KEGG GLYCAN (2)   
Chemical reaction (5)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Gene (1008)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (351)   
   KEGG MGENES (288)   
   RefGene (368)   
Protein sequence (768)   
   UniProt (756)   
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DNA sequence (29)   
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