KEGG   ENZYME: 3.1.4.54
Entry
EC 3.1.4.54                 Enzyme                                 
Name
N-acetylphosphatidylethanolamine-hydrolysing phospholipase D;
NAPE-PLD;
anandamide-generating phospholipase D;
N-acyl phosphatidylethanolamine phospholipase D;
NAPE-hydrolyzing phospholipase D
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
Sysname
N-acetylphosphatidylethanolamine phosphatidohydrolase
Reaction(IUBMB)
N-acylphosphatidylethanolamine + H2O = N-acylethanolamine + a 1,2-diacylglycerol 3-phosphate
Substrate
N-acylphosphatidylethanolamine;
H2O [CPD:C00001]
Product
N-acylethanolamine;
1,2-diacylglycerol 3-phosphate
Comment
This enzyme is involved in the biosynthesis of anandamide. It does not hydrolyse phosphatidylcholine and phosphatidylethanolamine [1]. No transphosphatidation [1]. The enzyme contains Zn2+ and is activated by Mg2+ or Ca2+ [2].
History
EC 3.1.4.54 created 2011
Orthology
K13985  N-acyl-phosphatidylethanolamine-hydrolysing phospholipase D
Genes
HSA222236(NAPEPLD)
PTR472476(NAPEPLD)
PPS100985918(NAPEPLD)
GGO101137253(NAPEPLD)
PON100172099(NAPEPLD)
NLE100597657(NAPEPLD)
MCC695318(NAPEPLD)
MCF101866055(NAPEPLD)
CSAB103226680(NAPEPLD)
CATY105583185(NAPEPLD)
PANU101002201(NAPEPLD)
TGE112620104(NAPEPLD)
RRO104673548(NAPEPLD)
RBB108515835(NAPEPLD) 108516781
TFN117065567 117073126(NAPEPLD)
PTEH111526685(NAPEPLD)
CJC100398969(NAPEPLD)
SBQ101035720(NAPEPLD)
CSYR103257398(NAPEPLD)
MMUR105861562(NAPEPLD)
OGA100950255(NAPEPLD)
MMU242864(Napepld)
MCAL110294541(Napepld)
MPAH110317044(Napepld)
RNO296757(Napepld)
MCOC116097512(Napepld)
MUN110542463(Napepld)
CGE100759349(Napepld)
PLEU114707983(Napepld)
NGI103732204(Napepld)
HGL101720071 101722949(Napepld)
CPOC100724473(Napepld)
CCAN109683824(Napepld)
DORD106000064(Napepld)
DSP122111851(Napepld)
OCU100352578(NAPEPLD)
OPI101517920(NAPEPLD)
TUP102497728(NAPEPLD)
CFA100685330(NAPEPLD)
VVP112925355(NAPEPLD)
VLG121501701(NAPEPLD)
AML100480963(NAPEPLD)
UMR103658645(NAPEPLD)
UAH113258971(NAPEPLD)
UAR123789373(NAPEPLD)
ELK111155331
LLV125080389
MPUF101693336(NAPEPLD)
ORO101382909(NAPEPLD)
EJU114220549(NAPEPLD)
ZCA113911252(NAPEPLD)
MLX118020660(NAPEPLD)
FCA101090597(NAPEPLD)
PYU121028836(NAPEPLD)
PBG122487786(NAPEPLD)
PTG102971600(NAPEPLD)
PPAD109268121(NAPEPLD)
AJU106972731(NAPEPLD)
HHV120236339(NAPEPLD)
BTA541291(NAPEPLD)
BOM102267222(NAPEPLD)
BIU109557357(NAPEPLD)
BBUB102393342(NAPEPLD)
CHX102189544(NAPEPLD)
OAS101119039(NAPEPLD)
ODA120878152(NAPEPLD)
CCAD122438280(NAPEPLD)
SSC100737731(NAPEPLD)
CFR102512680(NAPEPLD)
CBAI105062398(NAPEPLD)
CDK105093013(NAPEPLD)
VPC102533294(NAPEPLD)
BACU103008372(NAPEPLD)
LVE103084546(NAPEPLD)
OOR101288700(NAPEPLD)
DLE111164657(NAPEPLD)
PCAD102983637(NAPEPLD)
PSIU116759345(NAPEPLD)
ECB100050247(NAPEPLD)
EPZ103541734(NAPEPLD)
EAI106837113(NAPEPLD)
MYB102251197(NAPEPLD)
MYD102751565(NAPEPLD)
MMYO118666032(NAPEPLD)
MLF102424257(NAPEPLD)
MNA107542318(NAPEPLD)
PKL118714382(NAPEPLD)
HAI109389513(NAPEPLD)
DRO112303319(NAPEPLD)
SHON118994445(NAPEPLD)
AJM119034686 119065612(NAPEPLD)
PDIC114507561(NAPEPLD)
PHAS123811393(NAPEPLD)
MMF118620225(NAPEPLD)
RFQ117012521(NAPEPLD)
PALE102890709(NAPEPLD)
PGIG120591491(NAPEPLD)
PVP105295502(NAPEPLD)
RAY107511511(NAPEPLD)
MJV108386630(NAPEPLD)
TOD119244958(NAPEPLD)
SARA101542910(NAPEPLD)
LAV100654395(NAPEPLD)
TMU101361415
DNM101413796(NAPEPLD)
MDO100017822(NAPEPLD)
GAS123248686(NAPEPLD)
SHR100922754(NAPEPLD)
PCW110206980(NAPEPLD)
OAA100076416(NAPEPLD)
GGA417719(NAPEPLD)
PCOC116229857(NAPEPLD)
MGP100544476(NAPEPLD)
CJO107323995(NAPEPLD)
NMEL110392346(NAPEPLD)
APLA101804191(NAPEPLD)
ACYG106035555(NAPEPLD)
AFUL116486589(NAPEPLD)
TGU100232210(NAPEPLD)
LSR110479099(NAPEPLD)
SCAN103813530(NAPEPLD)
PMOA120509262(NAPEPLD)
OTC121336296(NAPEPLD)
PRUF121357434(NAPEPLD)
GFR102032062(NAPEPLD)
FAB101814848(NAPEPLD)
PHI102109609(NAPEPLD)
PMAJ107204704(NAPEPLD)
CCAE111923262(NAPEPLD)
CCW104686879(NAPEPLD)
ETL114063925(NAPEPLD)
ZAB102065561(NAPEPLD)
FPG101919393(NAPEPLD)
FCH102047018(NAPEPLD)
CLV102087617(NAPEPLD)
EGZ104128561(NAPEPLD)
NNI104010736(NAPEPLD)
ACUN113475913(NAPEPLD)
TALA104362254(NAPEPLD)
PADL103920585(NAPEPLD)
ACHC115341143(NAPEPLD)
AAM106485772(NAPEPLD)
AROW112967029(NAPEPLD)
NPD112953318(NAPEPLD)
DNE112981770(NAPEPLD)
ASN102388069(NAPEPLD)
AMJ102564505(NAPEPLD)
CPOO109315744(NAPEPLD)
GGN109292157(NAPEPLD)
PSS102451163(NAPEPLD)
CMY102934785(NAPEPLD)
CPIC101938499(NAPEPLD)
TST117875128(NAPEPLD)
CABI116821392(NAPEPLD)
MRV120395226(NAPEPLD)
ACS100559466(napepld)
PVT110077181(NAPEPLD)
SUND121931206(NAPEPLD)
PBI103056311(NAPEPLD)
PMUR107301910 107303260
TSR106557310(NAPEPLD)
PGUT117675214(NAPEPLD)
VKO123020048(NAPEPLD)
PMUA114605284(NAPEPLD)
ZVI118091022(NAPEPLD)
GJA107113713(NAPEPLD)
XLA100036998(napepld.L) 100137693(napepld.S)
XTR100494898(napepld)
NPR108790209(NAPEPLD)
RTEM120931752(NAPEPLD)
BBUF120982385(NAPEPLD)
BGAR122927865(NAPEPLD)
DRE568061(napepld)
SRX107732974 107738826
SANH107694176
SGH107576752 107590591(napepld)
CAUA113062379(napepld)
AMEX103040918(napepld)
EEE113588094(napepld)
TRU101065463(napepld)
TNGGSTEN00014499G001
LCO104940541(napepld)
NCC104962590(napepld)
CGOB115028124(napepld)
ELY117249268(napepld)
SLUC116060068(napepld)
ECRA117938867(napepld)
PFLV114549885(napepld)
GAT120818163(napepld)
PPUG119210514(napepld)
MSAM119917947 119918362
CUD121505405(napepld)
MZE101469541(napepld)
ONL100712367(napepld)
OAU116309866(napepld)
OLA101162968(napepld)
OML112158646(napepld)
XMA102223768(napepld)
XCO114161161(napepld)
XHE116737174(napepld)
PRET103459295(napepld) 103459303 103459307 103459310
PFOR103146197 103146501
PLAI106940535 106943693
PMEI106922250 106924678(napepld) 106926645
GAF122826350(napepld)
CVG107095995(napepld)
CTUL119786199(napepld)
GMU124882656(napepld)
NFU107385164(napepld)
KMR108235103(napepld)
ALIM106521795(napepld)
NWH119428407(napepld)
AOCE111571463(napepld)
CSEM103382793(napepld)
POV109632910(napepld)
SSEN122767007(napepld)
HHIP117757647(napepld)
LCF108894945(napepld)
SDU111224580 111225006
SLAL111663671(napepld)
XGL120799172(napepld)
HCQ109520041(napepld)
BPEC110156007(napepld)
MALB109959912(napepld)
SASA106592130 106592131 106599773
OTW112240578
OMY110511372 110511373 110512490 110527029
OGO123994349 123995859 124020953
SALP112076497(napepld)
SNH120032121 120032140
ELS105023099(napepld)
SFM108920852 108931347
PKI111843163(napepld)
AANG118231513(napepld)
LOC102688045(napepld)
PSPA121318371 121319729
ARUT117415050 117419443
LCM102353963(NAPEPLD)
CMK103176983(napepld)
RTP109922755(napepld)
BFO118424143
BBEL109474584
SPU577498
APLC110974316
SKO100371650 100371802 100371950
ISCIscW_ISCW005989
DSV119431609
RSAN119372014
RMP119163260
TUT107363395
DPTE113797789
DFR124492981
CSCU111613185
PTEP107448406
SDM118182524
CELCELE_Y37E11AR.3(nape-2) CELE_Y37E11AR.4(nape-1)
NAINECAME_07618
TSPTsp_11558
PCAN112565234
BGT106051542 106064809 106073108
GAE121367940 121368412
HRF124134531 124134547
HRJ124258615 124258699
CRG105331080 105334696
MYI110442510 110443829 110443904 110443905
PMAX117330860 117331405 117331408 117331409
MMER123529856 123542890 123548809
OBI106876738
OSN115225289
LAK106172214
EGLEGR_04153 EGR_10923
NVE5511122 5511149
EPA110243010 110243011
ATEN116305450 116305457
ADF107340107 107341384 107341416
AMIL114959129 114959130 114962193
PDAM113672521 113672530 113672531
SPIS111325046 111325053 111325068
DGT114521952 114544427
HMG100205873 100208076 105846065
TADTRIADDRAFT_27102
QSU112001273 112022048 112029150
SMOSELMODRAFT_2635 SELMODRAFT_2636
CRECHLRE_01g007300v5
MNGMNEG_0591
CSLCOCSUDRAFT_49353
CVRCHLNCDRAFT_25371
APROF751_5638
CMECYME_CMG164C
GSLGasu_18170 Gasu_61510
CCPCHC_T00004230001
SCEYPL103C(FMP30)
AGOAGOS_ABR187C
ERCEcym_7074
KLAKLLA0_E24377g
KMXKLMA_10803(FMP30)
LTHKLTH0E13464g
VPOKpol_242p7
ZROZYRO0F14894g
CGRCAGL0H05885g
NCSNCAS_0C01900(NCAS0C01900)
NDINDAI_0E02620(NDAI0E02620)
TPFTPHA_0A01240(TPHA0A01240)
TBLTBLA_0B04420(TBLA0B04420)
TDLTDEL_0A05260(TDEL0A05260)
TGBHG536_0A06820
KAFKAFR_0E00700(KAFR0E00700)
ZMKHG535_0H02810
PPAPAS_chr3_1013
DHADEHA2G09240g
PICPICST_38719
PGUPGUG_02733
SPAASPAPADRAFT_53094
LELLELG_01488
CALCAALFM_C100470CA(CaO19.6061)
CTPCTRG_04313
COTCORT_0B07020
CDUCD36_00400
CTENCANTEDRAFT_119103
YLIYALI0B18876g
CLUCLUG_02090
CLUSA9F13_02g02035
CAURCJI97_003243
SLBAWJ20_2454(FMP30)
PKZC5L36_0D02540
BNNFOA43_001221
BBRXBRETT_002361
NCRNCU07271
NTENEUTE1DRAFT116607(NEUTE1DRAFT_116607)
SMPSMAC_12092
PANPODANS72p122 PODANSg3311
TTTTHITE_2114763
MTMMYCTH_2065104 MYCTH_2298369
CTHRCTHT_0035210
MGRMGG_13254
TMNUCRPA7_4696
SSCKSPSK_06335 SPSK_09259
FGRFGSG_09261
FPUFPSE_09653
FVRFVEG_03849
FOXFOXG_05981
NHENECHADRAFT_103573
TRETRIREDRAFT_56499
TRRM419DRAFT_72821
MAWMAC_03410
MAJMAA_02008
CMTCCM_00300
PLJVFPFJ_04106 VFPFJ_04707
VALVDBG_08193
VDAVDAG_10086
CFJCFIO01_10131
SAPOSAPIO_CDS5447
ELAUCREL1_1018 UCREL1_6338
PFYPFICI_04456 PFICI_06131
SSLSS1G_14439
BFUBCIN_11g00380
MBEMBM_06035
PSCOLY89DRAFT_578857 LY89DRAFT_601819 LY89DRAFT_602524 LY89DRAFT_721779
GLZGLAREA_01828 GLAREA_03112
ANGANI_1_804114(An13g03380)
TRGTRUGW13939_01286
ABEARB_05202
TVETRV_03618
PNOSNOG_02097
PTEPTT_03276 PTT_18658
BZECOCCADRAFT_8143 COCCADRAFT_85723
BSCCOCSADRAFT_338759 COCSADRAFT_39487
BORCOCMIDRAFT_32346 COCMIDRAFT_8345
AALTCC77DRAFT_1028734 CC77DRAFT_1099505
ZTRMYCGRDRAFT_51107 MYCGRDRAFT_95030
PFJMYCFIDRAFT_203906 MYCFIDRAFT_46081
BCOMBAUCODRAFT_67574 BAUCODRAFT_68318 BAUCODRAFT_76981
NPAUCRNP2_3901
TMLGSTUM_00011077001
CNECNA02140 CNC04610 CND01370
CNBCNBA2030 CNBC2560 CNBD4940 CNBD5270
CGICGB_B2500C CGB_C6720W CGB_D3590W
TMSTREMEDRAFT_29049 TREMEDRAFT_46031 TREMEDRAFT_62255
TASAA1Q1_05627 A1Q1_08094
TVSTRAVEDRAFT_51812 TRAVEDRAFT_51813
DSQDICSQDRAFT_87478
PCOPHACADRAFT_211339 PHACADRAFT_255196 PHACADRAFT_255223 PHACADRAFT_260146
SHSSTEHIDRAFT_120244 STEHIDRAFT_66170
HIRHETIRDRAFT_474791
ADLAURDEDRAFT_115787 AURDEDRAFT_43365
FMEFOMMEDRAFT_153567 FOMMEDRAFT_170545
GTRGLOTRDRAFT_75241
LBCLACBIDRAFT_256061
MRRMoror_3315
CCICC1G_02616
SCMSCHCODRAFT_31015
CPUTCONPUDRAFT_136233
SLASERLADRAFT_477716
UMAUMAG_01873 UMAG_02828 UMAG_05349 UMAG_05894
PFPPFL1_01859 PFL1_02654 PFL1_05388 PFL1_06682
MGLMGL_3806
PGRPGTG_12284
MLRMELLADRAFT_45981
SREPTSG_12980
DDIDDB_G0271270 DDB_G0293392
DPPDICPUDRAFT_28013 DICPUDRAFT_93840
DFADFA_03394
ACANACA1_062470 ACA1_203030
TANTA19325
TPVTP01_0189
TOTTOT_010000176
BEQBEWA_032550
BBOBBOV_I004470(19.m02152)
BMICBMR1_02g00400
TGOTGME49_231150
SMINv1.2.018948.t2(symbB.v1.2.018948.t2)
PTIPHATRDRAFT_24223
FCYFRACYDRAFT_151604
SPARSPRG_10673 SPRG_18228 SPRG_18230
GTTGUITHDRAFT_69665 GUITHDRAFT_93321
NGRNAEGRDRAFT_75174 NAEGRDRAFT_77977
TVATVAG_290900 TVAG_453240
PEZHWQ56_11510
MADHP15_2823
MARAD0851_07545
ILOIL1762
ILIK734_08875
IDTC5610_03410
LSDEMK97_14560
THTE2K93_16465
THAPFNC98_15595
PHAPSHAa0169
PTNPTRA_a0186
PSMPSM_A0187
PSEOOM33_17880
PIAPI2015_0185
PPHEPP2015_808
PBWD172_001185
PLZS4054249_02610 S4054249_22745
PALNB0W48_09365
PEAPESP_a0215
PSPOPSPO_b1084
PARTPARC_a0183
PTUPTUN_b0121
PNGPNIG_a0190
PTDPTET_a0194
PDJD0907_01310
PAGAPAGA_a0212
PCARPC2016_2986
PMAACPA52_01010
PXIJ5O05_03005
PSYMJ1N51_11295
AMCMADE_1018935
AMHI633_19490
AMAAamad1_18895
AMALI607_17835
AMAEI876_18210
AMAOI634_17975
AMADI636_18040
AMAII635_18885
AMAGI533_17770
AMACMASE_17910
AMBAMBAS45_18335
AMGAMEC673_18040
AMKAMBLS11_17410
SALKFBQ74_06370
GNIGNIT_0043
GPSC427_2096
LALAT746_11210
SALHHMF8227_01069
HMIsoil367_05125
SPOIIMCC21906_02843
ALGAQULUS_13680
LSSNCTC12082_01366
FTWFTW_2008
FTLFTL_1950
FTHFTH_1868
FTAFTA_2061
FTSF92_10810
FTIFTS_1897
FTOX557_09965
FTCDA46_767
FTVCH67_226
FTZCH68_2110
FTMFTM_1736
FTNFTN_1765
FTXAW25_219
FTDAS84_718
FTYCH70_134
FCFFNFX1_1803
FPHFphi_0827
FPTBZ13_1205
FPIBF30_1042(napepld)
FPMLA56_1351
FPXKU46_513
FPZLA55_2077
FPJLA02_429
FRTF7308_1964
FNAOOM_1825
FNLM973_00270
FRFLO80_06055
FRCKX01_258
FADCDH04_09365
FMIF0R74_08375
FSRKQR59_09365
TEETel_09040
ABOABO_0101
ADIB5T_00060 B5T_02787
APACS7S_17905
ALNAS19_01030
AXEP40_00310
KAKKalk_03860
OAIOLEAN_C27180
OMEOLMES_4758
VFFVITFI_CDS3377
CHRIDK842_16915
CHRBDK843_22445
AMAHDLM_1168
BYIBYI23_B000060
BUEBRPE67_BCDS02510
BUMAXG89_15555
BCAIK788_0003187
PHSC2L64_25755
PTERC2L65_22655
BUOBRPE64_BCDS11540
LIMNHKT17_08305
RSBRS694_06930
RHYRD110_02690
RHGEXZ61_01570 EXZ61_07110
POSDT070_05105
POOF7R28_04345
ACKC380_23355
ACRABSY15_1272
MMSmma_0705
HSEHsero_1817
HSZACP92_09060
HHTF506_17665
HEEhmeg3_16470
HHFE2K99_09185
HFRG5S34_10005
MTIMDIR46_01720
AONDEH84_11345
XYKGT347_14540
URUDSM104443_04276
DPSDP2297
DALDalk_0140
DTOTOL2_C00340
DWDDSCW_28320
DALKDSCA_26370
SCLsce9325
SCUSCE1572_52605
CCROCMC5_014210
SATSYN_00874
DAODesac_1424
SFUSfum_0514
PLAPlav_0016
PMOBHG718_12890
RBSRHODOSMS8_03259
LAPACP90_05215
LABPFJ695_07990
LABTFIU93_10855
PHQD1820_17125
LEJETW24_01455
NOGGKE62_04725
RCERC1_2067
FERFNB15_18410
TXITH3_08165
THACCSC3H3_07835
TIIDY252_11050
PUBSAR11_0306
PEGE5R92_04790
APMHIMB5_00014150
BBABd2858
BBATBdt_2799
BBWBDW_10500
BDZDOM22_04555
SNGSNE_A04770(napEPLD-A) SNE_A09680(napEPLD-B)
LUOHHL09_08645
LBILEPBI_I0728
LBFLBF_0705
TPXTurpa_3182
CABYCabys_805
TIDThein_1655
TAVG4V39_08360
TMAIFVE67_06100
 » show all
Reference
1  [PMID:14634025]
  Authors
Okamoto Y, Morishita J, Tsuboi K, Tonai T, Ueda N
  Title
Molecular characterization of a phospholipase D generating anandamide and its congeners.
  Journal
J Biol Chem 279:5298-305 (2004)
DOI:10.1074/jbc.M306642200
  Sequence
[hsa:222236] [mmu:242864] [rno:296757]
Reference
2  [PMID:16527816]
  Authors
Wang J, Okamoto Y, Morishita J, Tsuboi K, Miyatake A, Ueda N
  Title
Functional analysis of the purified anandamide-generating phospholipase D as a member of the metallo-beta-lactamase family.
  Journal
J Biol Chem 281:12325-35 (2006)
DOI:10.1074/jbc.M512359200
  Sequence
[rno:296757] [mmu:242864] [hsa:222236]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.4.54
IUBMB Enzyme Nomenclature: 3.1.4.54
ExPASy - ENZYME nomenclature database: 3.1.4.54
BRENDA, the Enzyme Database: 3.1.4.54

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