| Entry |
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| Name |
phosphonopyruvate hydrolase;
PPH
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| Class |
Hydrolases;
Acting on carbon-phosphorus bonds;
Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
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| Reaction(IUBMB) |
3-phosphonopyruvate + H2O = pyruvate + phosphate [RN: R08200]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Highly specific for phosphonopyruvate as substrate [2]. The reaction is not inhibited by phosphate but is inhibited by the phosphonates phosphonoformic acid, hydroxymethylphosphonic acid and 3-phosphonopropanoic acid [2]. The enzyme is activated by the divalent cations Co2+, Mg2+ and Mn2+. This enzyme is a member of the phosphoenolpyruvate mutase/isocitrate lyase superfamily [3].
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| History |
EC 3.11.1.3 created 2007
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| Pathway |
| ec00440 | Phosphonate and phosphinate metabolism |
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| Orthology |
| K19669 | phosphonopyruvate hydrolase |
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| Genes |
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| Reference |
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| Authors |
Ternan NG, Hamilton JT, Quinn JP. |
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| Title |
Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6. |
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| Journal |
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| Reference |
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| Authors |
Kulakova AN, Wisdom GB, Kulakov LA, Quinn JP |
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| Title |
The purification and characterization of phosphonopyruvate hydrolase, a novel carbon-phosphorus bond cleavage enzyme from Variovorax sp Pal2. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Chen CC, Han Y, Niu W, Kulakova AN, Howard A, Quinn JP, Dunaway-Mariano D, Herzberg O |
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| Title |
Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 3.11.1.3 |
| ExPASy - ENZYME nomenclature database: | 3.11.1.3 |
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