KEGG   ENZYME: 3.2.1.107
Entry
EC 3.2.1.107                Enzyme                                 
Name
protein-glucosylgalactosylhydroxylysine glucosidase;
PGGHG (gene name);
2-O-alpha-D-glucopyranosyl-5-O-alpha-D-galactopyranosylhydroxy-L-lysine glucohydrolase;
protein-alpha-D-glucosyl-1,2-beta-D-galactosyl-L-hydroxylysine glucohydrolase;
protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine glucohydrolase
Reaction(IUBMB)
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine + H2O = D-glucose + [collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine [RN:R04503]
Reaction(KEGG)
R04503
Substrate
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine;
H2O [CPD:C00001]
Product
D-glucose [CPD:C00031];
[collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
Comment
The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.
History
EC 3.2.1.107 created 1984
Orthology
K22078  protein-glucosylgalactosylhydroxylysine glucosidase
Genes
HSA80162(PGGHG)
PTR743704(PGGHG)
PPS100971508(PGGHG)
GGO109025438(PGGHG)
PON100454348(PGGHG)
NLE100601940(PGGHG)
HMH116466561(PGGHG)
MCC697199(PGGHG)
MCF101926478(PGGHG)
MTHB126936558
MNI105494123(PGGHG)
CSAB103236799(PGGHG)
CATY105577182(ATHL1)
PANU100997983(PGGHG)
TGE112606324(PGGHG)
MLEU105530810(ATHL1)
RRO104655560(PGGHG)
RBB108537993(PGGHG)
TFN117081588(PGGHG)
PTEH111520543(PGGHG)
CANG105503186(ATHL1)
CJC100385744(PGGHG)
SBQ101049147(ATHL1)
CIMI108298302(PGGHG)
CSYR103255720(PGGHG)
MMUR105874073(PGGHG)
LCAT123642985(PGGHG)
PCOQ105816546(ATHL1)
OGA100942793(PGGHG)
MMU212974(Pgghg)
MCAL110299030(Pgghg)
MPAH110325920(Pgghg)
RNO309103(Pgghg)
MCOC116103197(Pgghg)
ANU117697618(Pgghg)
MUN110553930(Pgghg)
CGE100757546(Pgghg)
MAUA101841403(Pgghg)
PROB127228517(Pgghg)
PLEU114693487(Pgghg)
MORG121457716(Pgghg)
MFOT126498799
AAMP119801636(Pgghg)
NGI103751062(Pgghg)
HGL101723837(Pgghg)
CPOC100719730(Pgghg)
CCAN109698993(Pgghg)
DORD105998608(Athl1)
DSP122095013(Pgghg)
NCAR124960687
OCU103347496
OPI101515994(PGGHG)
TUP102501509(PGGHG)
GVR103602145(ATHL1)
CFA483395(PGGHG)
CLUD112663545(PGGHG)
VVP112914422(PGGHG)
VLG121471379(PGGHG)
AML100469466(PGGHG)
UMR103671029(PGGHG)
UAH113248810(PGGHG)
UAR123775496(PGGHG)
ELK111150122
LLV125078262
MPUF101675114(ATHL1)
NVS122913206(PGGHG)
ORO101386980(ATHL1)
EJU114201538(PGGHG)
ZCA113914867(PGGHG)
MLX118016230(PGGHG)
NSU110582230(PGGHG)
LWW102745174(PGGHG)
FCA101087100(PGGHG)
PYU121022771(PGGHG)
PBG122484191(PGGHG)
LRUF124527555
PTG102949755(PGGHG)
PPAD109259816(PGGHG)
PUC125937748
AJU106987954(PGGHG)
HHV120223433(PGGHG)
BTA511919(PGGHG)
BOM102276155(PGGHG)
BBUB102405787(PGGHG)
BBIS104983406(ATHL1)
CHX102188292(PGGHG)
OAS101106382(PGGHG)
ODA120874199(PGGHG)
CCAD122430802(PGGHG)
SSC100518005(PGGHG)
CFR102511585(PGGHG)
CBAI105064430(ATHL1)
CDK105105739(PGGHG)
VPC102541806(PGGHG)
BACU103010340(PGGHG)
LVE103071239(ATHL1)
OOR101279414(ATHL1)
DLE111170281(PGGHG)
PCAD102991703(PGGHG)
PSIU116757783(PGGHG)
ECB100146757(PGGHG)
EPZ103568056(PGGHG)
EAI106843884(PGGHG)
MYB102252209(PGGHG)
MYD102760826(PGGHG)
MMYO118649081(PGGHG)
MNA107544451(ATHL1)
PKL118712997(PGGHG)
HAI109378078(PGGHG)
DRO112317246(PGGHG)
SHON118974897(PGGHG)
AJM119060967(PGGHG)
PDIC114499171(PGGHG)
PHAS123807371(PGGHG)
MMF118643163(PGGHG)
RFQ117030533(PGGHG)
PALE102887768(PGGHG)
PGIG120614156(PGGHG)
PVP105309813(PGGHG)
RAY107506507(ATHL1)
MJV108384850(PGGHG)
TOD119249819(PGGHG)
SARA101553230(ATHL1)
LAV100655235(PGGHG)
TMU101349759
ETF101643584(PGGHG)
DNM101410876(PGGHG)
MDO103092300(PGGHG)
GAS123252860(PGGHG)
SHR100923289(PGGHG)
AFZ127539999
PCW110203719(PGGHG)
OAA100091579(PGGHG)
GGA422991(PGGHG)
PCOC116237919(PGGHG)
MGP100538987(PGGHG)
CJO107314276(PGGHG)
NMEL110401494(PGGHG)
APLA101791880(PGGHG)
ACYG106047048(PGGHG)
AFUL116490363(PGGHG)
TGU100221918(PGGHG)
LSR110467551(PGGHG)
SCAN103827054(PGGHG)
PMOA120500985(PGGHG)
OTC121334793(PGGHG)
PRUF121352365(PGGHG)
GFR102035352(PGGHG)
FAB101806950(PGGHG)
PHI102112573(ATHL1)
PMAJ107205256(PGGHG)
CCAE111930573(PGGHG)
CCW104684542(PGGHG)
CBRC103614577(PGGHG)
ETL114063280(PGGHG)
ZAB102072796(PGGHG)
ACHL103805928(ATHL1)
SVG106854807(ATHL1)
FPG101919210(PGGHG)
FCH102051782(PGGHG)
CLV102089207(PGGHG)
EGZ104127885(ATHL1)
NNI104011413(ATHL1)
PCRI104034685(ATHL1)
PCAO104047290(ATHL1)
ACUN113481198(PGGHG)
TALA104365197(PGGHG)
PADL103925396(ATHL1)
AFOR103906296(PGGHG)
ACHC115351889(PGGHG)
HALD104316724(ATHL1)
HLE104832093(ATHL1)
AGEN126047544
GCL127016727
CCRI104169557(ATHL1)
MUI104546391(ATHL1)
FGA104077869(ATHL1)
GSTE104253621(ATHL1)
LDI104352058(ATHL1)
OHA104333131(ATHL1)
NNT104407864(ATHL1)
SHAB115607583(PGGHG)
DPUB104297526(ATHL1)
ACAR104529766(PGGHG)
CPEA104392123(ATHL1)
AVIT104277475(ATHL1)
CVF104282478(ATHL1)
CUCA104057242(ATHL1)
BRHI104489962(ATHL1)
AAM106489147(PGGHG)
AROW112973763(PGGHG)
NPD112948025(PGGHG)
TGT104574673(ATHL1)
DNE112993815(PGGHG)
SCAM104149783(ATHL1)
ASN102382785(PGGHG)
AMJ102566701(PGGHG)
CPOO109318166(PGGHG)
GGN109299260(PGGHG)
PSS102452333(PGGHG)
CMY102947357(PGGHG)
CPIC101949278(PGGHG)
TST117877389(PGGHG)
CABI116822732(PGGHG)
MRV120403851(PGGHG)
ACS100552045(pgghg)
PVT110086184(PGGHG)
SUND121919827(PGGHG)
PBI103064802(PGGHG)
PMUR107291105(PGGHG)
CTIG120312470(PGGHG)
PGUT117665147(PGGHG)
VKO123021215(PGGHG)
PMUA114600382(PGGHG)
ZVI118078924 118088834(PGGHG)
GJA107112736(PGGHG)
STOW125425776(PGGHG)
XLA108705477(pgghg.L)
XTR100496278(pgghg)
NPR108784117(PGGHG)
RTEM120917544(PGGHG)
BBUF120980623(PGGHG)
BGAR122920195(PGGHG)
DRE777617(pgghg)
SRX107726850(athl1) 107754371
SANH107674819
SGH107551409
CCAR109071510(pgghg)
CAUA113043029
CGIB128014171
PPRM120473357
MAMB125246848(pgghg)
CIDE127506684
MASI127435747
IPU108274703(pgghg)
PHYP113529799(pgghg)
SMEO124392887(pgghg)
TFD113653967(pgghg)
AMEX103031131(pgghg)
EEE113584482(pgghg)
TRU101062094(pgghg)
TNGGSTEN00029865G001
LCO104928845(pgghg)
NCC104956195(athl1)
CGOB115009887(pgghg)
ELY117261814(pgghg)
EFO125887913(pgghg)
PLEP121950353(pgghg)
SLUC116038726(pgghg)
ECRA117949357(pgghg)
ESP116676528 116694469
PFLV114560021(pgghg)
GAT120808687(pgghg)
PPUG119196680(pgghg)
MSAM119893919(pgghg)
CUD121515754(pgghg)
ALAT119024343(pgghg)
MZE101468880(pgghg)
ONL100691439(pgghg)
OAU116326290(pgghg)
OLA101155845(pgghg)
OML112152859(pgghg)
XMA102229355(pgghg)
XCO114136298(pgghg)
XHE116711420(pgghg)
PRET103466501(pgghg)
PFOR103141313(athl1)
PLAI106964669(athl1)
PMEI106933109(athl1)
GAF122835741(pgghg)
CVG107100632(athl1)
CTUL119787430(pgghg)
GMU124877816(pgghg)
NFU107388675(athl1)
KMR108229896(pgghg)
ALIM106521182(athl1)
NWH119415788(pgghg)
AOCE111577282(pgghg)
MCEP125014680(pgghg)
CSEM103380281(athl1)
POV109623963(pgghg)
SSEN122776450(pgghg)
HHIP117762923(pgghg)
HSP118110268(pgghg)
LCF108886949(pgghg) 108891288
SDU111229684(pgghg)
SLAL111667069(pgghg)
XGL120793538(pgghg)
HCQ109507601(pgghg)
SSCV125970104
BPEC110169653(pgghg)
MALB109972776(pgghg)
BSPL114857078(pgghg)
SASA106561823(pgghg)
STRU115197720(pgghg)
OTW112243931(pgghg)
OMY110501276(pgghg)
OGO124031677
SALP112071753(pgghg)
SNH120066505(pgghg)
CCLU121531582(pgghg)
ELS105029689(pgghg)
SFM108940848(pgghg)
PKI111848029(pgghg)
AANG118215147(pgghg)
PSPA121294971(pgghg)
ARUT117432325(pgghg)
PSEX120535647(pgghg)
LCM102363080(PGGHG)
CMK103174850(pgghg)
RTP109928234(pgghg)
BFO118422669 118427572
BBEL109464648 109485036
CIN100183627
SCLV120347404
SPU762741
APLC110983330
SKO100367252 100368173 100368199 100375069 102801557 102807261
DMEDmel_CG16965(CG16965)
DER6541527
DSE6617683
DSIDsimw501_GD23801(Dsim_GD23801)
DYADyak_GE18556
DSR110183998
DPO4817014
DPE6588680
DMN108162922
DWI6642582
DGR6566703
DMODmoj_GI24803
DAZ108611620
DNV108649416
DHE111603770
CCAT101461034
BOD106622467
RZE108366250
MDE101889101
SCAC106081277
LCQ111683711
LSQ119602617
AGAAgaP_AGAP008548
ACOZ120957616 120958356 120959147
AARA120900865 120901729 120901730
ASTE118512519 118512520 118512521
AAG5564618 5564619 5567690 5576505
AALB109417656 109417660 109417661 109417662 109622152 109622154 115255403 115255515
CQUCpipJ_CPIJ008851 CpipJ_CPIJ008852 CpipJ_CPIJ008853 CpipJ_CPIJ008854 CpipJ_CPIJ008855
CPII120424350 120424354 120424366 120424367 120424368
CNS116348022
BCOO119076367 119078597
BMOR101739191
BMAN114246045
MSEX115448110
DPLKGM_201303
BANY112045495
NIQ126781408
PMAC106718814
PPOT106101409
PXU106118146
PRAP110993009
PBX123707481
PNAP125056025
ZCE119828533
CCRC123706310
HAW110378758
HZE124646374
TNL113493163
SLIU111352767
OFU114356286
PXY105386635 105394851
FCD110848403
DPXDAPPUDRAFT_55687
DMK116932146
DPZ124349473
EAF111706613
HROHELRODRAFT_161910 HELRODRAFT_193290 HELRODRAFT_76967
LGILOTGIDRAFT_141357 LOTGIDRAFT_226064
PCAN112570055
BGT106054488 106072139
GAE121371587 121372683
HRF124122908 124124759
HRJ124254530 124291996
CRG105324264 105328484 105340679
MYI110440546 110440547 110440583
PMAX117322791 117324382 117324383
MMER123539149
DPOL127870704
OBI106873889
OSN115216968
LAK106179554
OVIT265_13014
NVE5507997 5508007 5510775 5511304
EPA110233900 110233920 110233925 110253303
ATEN116290584 116294426 116294435 116294905
ADF107342244 107356873 107356887
AMIL114956600 114956601 114965960
PDAM113668974 113668976 113674506
SPIS111321839 111346317 111346319
DGT114523848
XEN124434693
HMG100207176
MBRMONBRDRAFT_16873 MONBRDRAFT_22300
SREPTSG_06584
DDIDDB_G0287109(athl1)
DPPDICPUDRAFT_97948
DFADFA_10677(athl1)
ACANACA1_054500 ACA1_368900
NGRNAEGRDRAFT_69774(AM32)
NPYNPRO_19770
 » show all
Reference
1  [PMID:385589]
  Authors
Hamazaki H, Hotta K.
  Title
Purification and characterization of an alpha-glucosidase specific for hydroxylysine-linked disaccharide of collagen.
  Journal
J Biol Chem 254:9682-7 (1979)
Reference
2  [PMID:7460918]
  Authors
Hamazaki H, Hotta K.
  Title
Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens.
  Journal
Eur J Biochem 111:587-91 (1980)
DOI:10.1111/j.1432-1033.1980.tb04975.x
Reference
3  [PMID:385599]
  Authors
Sternberg M, Spiro RG.
  Title
Studies on the catabolism of the hydroxylysine-linked disaccharide units of basement membranes and collagens. Isolation and characterization of a rat kidney alpha-glucosidase of high specificity.
  Journal
J Biol Chem 254:10329-36 (1979)
Reference
4  [PMID:26682924]
  Authors
Hamazaki H, Hamazaki MH
  Title
Catalytic site of human protein-glucosylgalactosylhydroxylysine glucosidase: Three crucial carboxyl residues were determined by cloning and site-directed mutagenesis.
  Journal
Biochem Biophys Res Commun 469:357-62 (2016)
DOI:10.1016/j.bbrc.2015.12.005
  Sequence
[gga:422991]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.107
IUBMB Enzyme Nomenclature: 3.2.1.107
ExPASy - ENZYME nomenclature database: 3.2.1.107
BRENDA, the Enzyme Database: 3.2.1.107
CAS: 72829-45-9

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