KEGG ENZYME: 3.2.1.132

ENZYME: 3.2.1.132

Entry

EC 3.2.1.132                Enzyme

Name

chitosanase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

chitosan N-acetylglucosaminohydrolase

Reaction(IUBMB)

Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan

Reaction(KEGG)

(other) R02833 R06175(G)

Comment

A whole spectrum of chitosanases are now known (for more details, see {http://rbrzezinski.recherche.usherbrooke.ca/::http://rbrzezinski.recherche.usherbrooke.ca/}). They can hydrolyse various types of links in chitosan. The only constant property is the endohydrolysis of GlcN-GlcN links, which is common to all known chitosanases. One known chitosanase is limited to this link recognition [4], while the majority can also recognize GlcN-GlcNAc links or GlcNAc-GlcN links but not both. They also do not recognize GlcNAc-GlcNAc links in partly acetylated chitosan.

History

EC 3.2.1.132 created 1990, modified 2004

Pathway

ec00520

Amino sugar and nucleotide sugar metabolism

ec01100

Metabolic pathways

Orthology

K01233

chitosanase

Genes

BGT:	106060432 106073034

QSU:

112020272

FVR:	FVEG_01778

FOX:	FOXG_02920

TRE:	TRIREDRAFT_70341

TRR:	M419DRAFT_13025

MAW:

MAC_08097

MAJ:

MAA_09022

CMT:

CCM_09571

PLJ:	VFPFJ_07273 VFPFJ_10340

AFM:	AFUA_3G14980 AFUA_8G00930

ACT:	ACLA_006620 ACLA_016840

NFI:	NFIA_062130 NFIA_094360

AOR:	AO090011000027 AO090113000063 AO090124000065

ANG:	ANI_1_1412034(An03g05260) ANI_1_1844184(An04g04530)

AFV:	AFLA_007386 AFLA_009777

ALUC:

AKAW2_50899A AKAW2_51449S

APUU:

APUU_81074S

PCS:	Pc12g07820

PDP:	PDIP_35480

TRG:	TRUGW13939_04760

CIM:	CIMG_09773

CPW:	CPC735_023010

URE:	UREG_04688

ABE:

ARB_00766

TVE:

TRV_00085

ZTR:	MYCGRDRAFT_96467

NPA:	UCRNP2_3805

SHS:	STEHIDRAFT_112437

ADL:	AURDEDRAFT_130380 AURDEDRAFT_186562 AURDEDRAFT_74830

MPR:	MPER_11138

MRR:	Moror_13977

KMI:	VW41_11695

CIF:	AL515_22930

CARS:

E1B03_13925

CNT:	JT31_00610

CEM:	LH23_16605

CEN:	LH86_15590

LPY:	FIV34_09340

LANH:

KR767_12360

PALK:

PSAKL28_22030

PAGR:

E2H98_07390

SKH:	STH12_01428(csn)

SCYP:

JYB88_08930

SSEM:

JYB85_09110

CJA:

CJA_3747

GSN:	YC6258_05941

CVI:

CV_3931

CVC:	BKX93_10435

CHRO:

CXB49_01655

CHRI:

DK842_04725

CHRB:

DK843_10190

CRZ:	D1345_12490

CHAE:

CH06BL_25720

BGD:	bgla_2g09600

BGO:	BM43_4589(csn)

BRH:	RBRH_02856

PCOM:

NTU39_09075

PBH:	AAW51_2941

MIU:	ABE85_05140 ABE85_13390

JAG:	GJA_1109(csn) GJA_1110(csn) GJA_1111(csn) GJA_1112(csn) GJA_1802(csn) GJA_1803(csn)

CARE:

LT85_2580

CPRA:

CPter91_3397(csn)

UPV:	EJN92_11100

UPI:	EJG51_013730

NII:	Nit79A3_1025

NIM:

W01_16340

NARC:

NTG6680_0240

APET:

ToN1_40840

DAR:

Daro_2852

FLE:	KI610_14985

AZI:	AzCIB_1024

TMZ:	Tmz1t_3574

PLA:

Plav_0939

PMOB:

HG718_08915

RBS:	RHODOSMS8_00528(csn)

RAD:	CO657_11065

HNI:	W911_08845

MSD:	MYSTI_01268

MFB:	MFUL124B02_07090

CCX:	COCOR_06517

SUR:	STAUR_1941

AVM:	JQX13_15785

MBD:	MEBOL_002371

CFUS:

CYFUS_009189

PAUU:

E8A73_042520

BSU:	BSU26890(csn)

BSR:

I33_2730

BSL:

A7A1_0329

BSH:	BSU6051_26890(csn)

BSY:	I653_12750

BSUT:

BSUB_02870(csn)

BSUL:

BSUA_02870(csn)

BSUS:

Q433_14455

BSO:	BSNT_09076(csn)

BSN:	BSn5_04145

BSQ:	B657_26890(csn)

BSX:	C663_2522(csn)

BSP:	U712_13195

BST:

GYO_2921

BAY:	RBAM_029740

BAQ:	BACAU_3000(csn)

BYA:	BANAU_3164(csn)

BAMP:

B938_15235

BQY:	MUS_3567(csn)

BAML:

BAM5036_2888(csn)

BAMA:

RBAU_3106(csn)

BAMN:

BASU_2894(csn)

BAMB:

BAPNAU_3155(csn)

BAMT:

AJ82_16845

BAMY:

V529_32340(csn)

BMP:	NG74_03126(csn)

BAO:	BAMF_3066(csn)

BAZ:	BAMTA208_16520(csn)

BQL:	LL3_03344(csn)

BXH:	BAXH7_03372(csn)

BAMI:

KSO_004285

BAMC:

U471_30820

BAMF:

U722_16000

BAE:	BATR1942_06760

BVM:	B9C48_15205

BSON:

S101395_01714

BHT:	DIC78_03515

BMOJ:

HC660_12620(csn)

BTEQ:

G4P54_13750

BIQ:	AN935_13095

BCAB:

EFK13_13635(csn)

BJS:

MY9_2670

BACP:

SB24_13865

BACB:

OY17_18210

BACY:

QF06_11630

BACL:

BS34A_29350(csn)

BALM:

BsLM_2641

BGI:	BGM20_07335

TAID:

KS242_16075

SCAP:

AYP1020_2099(csn)

SSCH:

LH95_00710

SSCZ:

RN70_00920

SFF:	FOB90_07050

LGZ:	NCTC10812_00576(csn)

PPM:	PPSC2_18125(csn)

PPO:	PPM_3647(csn)

PPOL:

X809_34215

PPQ:	PPSQR21_036500

PPOY:

RE92_19075

POD:	PODO_19015

PAEJ:

H70737_19500

PIH:	UB51_10650

PPEO:

ABE82_18160

PLUT:

EI981_05310

PCHI:

PC41400_15865

LKO:	ABN16_06395

LACA:

LAC1533_0180

PCE:	PECL_1823(csn)

CBE:

Cbei_0949

CBZ:

Cbs_0949

CBEI:

LF65_01008

CDY:	F3K33_05010

CSH:	Closa_0438

SCO:	SCO0677(csn) SCO2024(SC3A3.02)

SALB:

XNR_0630 XNR_4855

SMA:	SAVERM_6191(csn2)

SSX:	SACTE_5457 SACTE_5458 SACTE_5886

SVL:	Strvi_2888

SHY:

SHJG_1996

SHO:	SHJGH_1761

SRC:	M271_11085

SMAL:

SMALA_6368

SSOI:

I1A49_35685

SFA:	Sfla_1047 Sfla_1048

SBH:

SBI_02563

SDV:	BN159_0394 BN159_0876(csn1) BN159_6425(csn3)

SALS:

SLNWT_0768 SLNWT_5869

STRP:

F750_5797 F750_5798

SFI:

SFUL_6175

SCI:	B446_03745

SLV:	SLIV_27590(csn1) SLIV_34555(csn2)

SGU:	SGLAU_09170(csn)

STRE:

GZL_02610 GZL_05266

SCW:	TU94_08700

SLD:

T261_1844

SLC:	SL103_25165

STRM:

M444_06945

SAMB:

SAM23877_2111 SAM23877_6866(csn)

SPRI:

SPRI_1051 SPRI_1545

SCX:	AS200_05585

SRW:	TUE45_00747(csn)

STRF:

ASR50_09875

SLE:	sle_51160(sle_51160)

SPAV:

Spa2297_08235 Spa2297_30295

STRT:

A8713_25850 A8713_29725

SCLF:

BB341_03975

SGS:	AVL59_26615

STSI:

A4E84_01680 A4E84_10470

SKY:	D0C37_04445 D0C37_26165

SALW:

CP975_08795 CP975_29090

SGM:	GCM10017557_15620 GCM10017557_61740

SNR:	SNOUR_11140(csn) SNOUR_24450

SALU:

DC74_6404

SALL:

SAZ_32170

SPLU:

LK06_031025

STRD:

NI25_01975 NI25_29045

SNW:	BBN63_04660 BBN63_05800 BBN63_05810

SAUO:

BV401_35805

SSIA:

A7J05_07810

SPUN:

BFF78_39905

SGV:	B1H19_31965

SALF:

SMD44_06848(csn)

SALJ:

SMD11_1083(csn)

SLX:	SLAV_07155(csn)

STRO:

STRMOE7_30450

SFK:

KY5_6563c

SNZ:	DC008_02340 DC008_08495

SGE:	DWG14_01829(csn_1) DWG14_08070(csn_2)

SRJ:

SRO_6811

SLK:	SLUN_31860

SDX:	C4B68_30085

SGD:	ELQ87_02320 ELQ87_29200

SCYA:

EJ357_02270 EJ357_11915

SAST:

CD934_25415

SNQ:	CP978_04735 CP978_06050

STIR:

DDW44_27010

SKA:	CP970_08325

SVN:	CP980_05670

SNK:	CP967_02505 CP967_04920 CP967_04925

SHAW:

CEB94_02140

SFIC:

EIZ62_04640

SGAL:

CP966_34370

SSPO:

DDQ41_28305

SVR:	CP971_26805

SPAD:

DVK44_29140 DVK44_30690

SFY:	GFH48_10305 GFH48_29825

SAQU:

EJC51_13695 EJC51_42250

SGF:	HEP81_07151

SRIM:

CP984_07750

SSUB:

CP968_06215

SPHV:

F9278_39015

SFUG:

CNQ36_08950

SPAC:

B1H29_02795 B1H29_27250

SCHA:

CP983_02365 CP983_32195

SCOE:

CP976_11765 CP976_40975

SSPB:

CP982_11915 CP982_32540

SNF:	JYK04_06923(csn_2)

SPLA:

CP981_30730

SBRO:

GQF42_05245

STUI:

GCM10017668_67470

SATA:

C5746_33485 C5746_33500

SHUN:

DWB77_01701(csn)

SCYG:

S1361_02500(csn)

SFEU:

IM697_11785 IM697_21255

SLIA:

HA039_06265 HA039_06270

SDW:	K7C20_29360

SVD:	CP969_11210

SMOB:

J7W19_31940

SPRA:

CP972_09260

SPEU:

CGZ69_31535

SXN:	IAG42_26530

SHK:	J2N69_30525

SDD:	D9753_33665

SACT:

DMT42_02225 DMT42_10105

SINE:

KI385_35315

SLF:	JEQ17_34360

SAOV:

G3H79_06570

SDUR:

M4V62_31025

SYAN:

NRK68_28990

SRUG:

F0345_05825

SNIG:

HEK616_48810

SVIO:

HWN34_03850 HWN34_24530

SCT:	SCAT_5429(csn)

SCY:	SCATT_54280

KSK:	KSE_15150(csn1) KSE_40860(csn2) KSE_40930(csn3)

KAU:	B6264_21365

KIT:	CFP65_1163

KIS:	HUT16_06310

STRI:

C7M71_008240

RSA:	RSal33209_3139

LMOI:

VV02_22645

AUS:	IPK37_19290

RAIN:

Rai3103_16670

KQI:	F1D05_07980

SRO:

Sros_2053

NOA:	BKM31_04180

NOW:	GBF35_34045

AMD:

AMED_6991

AMN:

RAM_35860

AMM:

AMES_6884

AMZ:

B737_6884

AOI:

AORI_1736

AJA:	AJAP_30805(csn)

AMYC:

CU254_28230

AORI:

SD37_32500

APRT:

MUY14_01600

APRE:

CNX65_21695

AMI:

Amir_4331

KAL:	KALB_2167 KALB_4388

KPHY:

AOZ06_37680

MICH:

FJK98_21295

ASE:	ACPL_2150(csn) ACPL_7579(csn2)

AMS:	AMIS_60150 AMIS_73130

ACTN:

L083_7237(csn2) L083_7833

AFS:	AFR_38200 AFR_41225

ACTS:

ACWT_2028 ACWT_7449

AIH:	Aiant_34180 Aiant_77800

ACTR:

Asp14428_51640 Asp14428_58090

DVC:	Dvina_07825 Dvina_29635 Dvina_33695 Dvina_42880

DROS:

Drose_07585

DAUR:

Daura_33040

DMAT:

Dmats_07340 Dmats_24830 Dmats_28590 Dmats_37570

CCAZ:

COUCH_02870 COUCH_06340

HBS:	IPV69_15965

CHIT:

FW415_23625

LACS:

H4075_07665

SPIK:

EXU85_18100

MHOR:

MSHOH_0861 MSHOH_1458 MSHOH_3609 MSHOH_3610 MSHOH_3611

» show all

Reference

Authors

Fenton, D.M. and Eveleigh, D.E.

Title

Purification and mode of action of a chitosanase from Penicillium islandicum.

Journal

J Gen Microbiol 126:151-165 (1981)

Reference

2 [PMID:10521473]

Authors

Saito J, Kita A, Higuchi Y, Nagata Y, Ando A, Miki K

Title

Crystal structure of chitosanase from Bacillus circulans MH-K1 at 1.6-A resolution and its substrate recognition mechanism.

Journal

J Biol Chem 274:30818-25 (1999)
DOI:10.1074/jbc.274.43.30818

Sequence

[ag:BAA01474]

Reference

3 [PMID:1368330]

Authors

Izume M, Nagae S, Kawagishi H, Mitsutomi M, Ohtakara A.

Title

Action pattern of Bacillus sp. no. 7-M chitosanase on partially N-acetylated chitosan.

Journal

Biosci Biotechnol Biochem 56:448-53 (1992)
DOI:10.1271/bbb.56.448

Reference

4 [PMID:8564542]

Authors

Marcotte EM, Monzingo AF, Ernst SR, Brzezinski R, Robertus JD

Title

X-ray structure of an anti-fungal chitosanase from streptomyces N174.

Journal

Nat Struct Biol 3:155-62 (1996)
DOI:10.1038/nsb0296-155

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.132

IUBMB Enzyme Nomenclature:

3.2.1.132

ExPASy - ENZYME nomenclature database:

3.2.1.132

BRENDA, the Enzyme Database:

3.2.1.132

CAS:	51570-20-8

DBGET integrated database retrieval system