KEGG ENZYME: 3.2.1.152

ENZYME: 3.2.1.152

Entry

EC 3.2.1.152                Enzyme

Name

mannosylglycoprotein endo-beta-mannosidase;
endo-beta-mannosidase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Reaction(IUBMB)

Hydrolysis of the alpha-D-mannosyl-(1->6)-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequence of glycoprotein to alpha-D-mannosyl-(1->6)-D-mannose and N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequences

Comment

The substrate group is a substituent on N-4 of an asparagine residue in the glycoprotein. The mannose residue at the non-reducing end of the sequence may carry further alpha-D-mannosyl groups on O-3 or O-6, but such a substituent on O-3 of the beta-D-mannosyl group prevents the action of the enzyme. The enzyme was obtained from the lily, Lilium longiflorum.

History

EC 3.2.1.152 created 2005

Orthology

K18577

mannosylglycoprotein endo-beta-mannosidase

Genes

ATH:

AT1G09010

ALY:

9325794

CRB:

17900667

CSAT:

104739491 104755088 104767262

EUS:	EUTSA_v10006730mg

BRP:	103836364 103871635

BNA:	106346294 106362335 106415431 106453177

BOE:	106294903 106307139

RSZ:	108807750 108822974 130507228

THJ:

104805875

CPAP:

110815331

CIT:

102618119

CIC:	CICLE_v10000150mg

PVY:	116118844 116128562

MINC:

123209656

TCC:

18600378

GRA:	105761023 105781536

GHI:	107887393 107893587 107920199 107958667

GAB:	108464371 108473723

HSYR:

120124249 120154013

DZI:	111277297 111295937

EGR:

104415555

GMX:	100787941 100804477 100809140 100817144

GSJ:	114370283 114400734 114402823 114407611

PVU:	PHAVU_006G092100g PHAVU_007G180100g

VRA:	106772076 106776242

VAR:	108342919 108345492

VUN:	114188276 114191121

VUM:	124836018 124837497

CCAJ:

109795453 109796169

APRC:

113861935 113872132

MTR:	11410041 11430551

TPRA:

123890901 123907349

CAM:	101503609 101505520

PSAT:

127098294 127132353

VVO:	131610606 131633928 131644094

LJA:	130718328 130731197

ADU:	107465393 107492112

AIP:	107618043 107646499

AHF:	112710838 112758141 112775615

LANG:

109330117 109336087

PCIN:

129295024 129295874

QSA:	O6P43_007519

FVE:

101296018

RCN:

112192351

PPER:

18775253 18775314

PMUM:

103344631 103344633

PAVI:

110759657

PDUL:

117632383 117632384

MDM:	103450943 103451325

MSYL:

126592900 126592901

PXB:

103942899

ZJU:

107412022

CSAV:

115718737

CSV:

101207955

CMO:

103492174

BHJ:

120069814

MCHA:

111015278

CMAX:

111468316

CMOS:

111433409

CPEP:

111792852

RCU:

8285565

MEAN:

126673362

JCU:

105647543

HBR:

110644505

MESC:

110620745

POP:

7481756

PEU:

105125043

PALZ:

118060785

PNZ:

133669615

JRE:

109008073

CILL:

122317306

CAVE:

132168921

QSU:

111983211

QLO:

115979009

TWL:	120008957 120008958

VVI:

100254646

VRI:

117930200

SLY:

101267848

SPEN:

107008011

SOT:

102586571

SSTN:

125863851

SDUL:

129886193

CANN:

107854888

LBB:

132645908

NTA:	107762938 107828361

NSY:

104218593

NTO:

104105594

NAU:

109209905

INI:

109181437

ITR:

116005572

SIND:

105169398 105172345

OEU:	111389164 111411182

EGT:	105960789 105976210 105976346

SSPL:

121795360 121798907

SMIL:

130996919

SHIS:

125205894

APAN:

127260842

HAN:

110920122

ECAD:

122590961

LSV:

111895788

CCAV:

112507203

DCR:	108204287 108227635

CSIN:

114310720

RVL:	131334524 131334525

AEW:

130783446

BVG:

104890840

SOE:

110798590

CQI:

110708616

ATRI:

130797300

MOF:

131152684

NNU:

104596494

MING:

122057744

TSS:

122642219

PSOM:

113315242

OSA:

4338683

OBR:

102703868

OGL:

127773687

BDI:

100834607

ATS:

109774075

TDC:	119277985 119341843

TAES:

123054439 123131312 123181888

TUA:

125516433

LPER:

127315760

LRD:	124647930 124707799

SBI:

8085086

ZMA:

103630319

SITA:

101785350

SVS:

117850534

PVIR:

120665611 120698819

PHAI:

112885781

PDA:

103716435

EGU:

105057570

MUS:

103977642

ZOF:	121988714 121997643

DCT:	110098097 110114017

PEQ:	110021821 110023222

AOF:

109840056

MSIN:

131226012

NCOL:

116258708

ATR:

18440882

SMO:	SELMODRAFT_155695 SELMODRAFT_164536

PPP:	112285782 112292500

CSL:	COCSUDRAFT_28678

TPS:	THAPSDRAFT_262343

EHX:	EMIHUDRAFT_231619

GTT:	GUITHDRAFT_64741

BUR:	Bcep18194_C7540

BCON:

NL30_34310

BMEC:

WJ16_19375

PMEG:

FNZ07_11640

BCEL:

BcellWH2_02649(csxA_3)

PMUC:

ING2E5A_0455(EBM)

PSAC:

PSM36_0848 PSM36_1079

PDI:

BDI_3381

PARC:

CI960_23000

AIT:	AI2BBH_10670

MCOS:

GM418_24495

CPI:

Cpin_2275

CHIH:

GWR21_26635

CFIL:

MYF79_27920

NKO:	Niako_7278

NSO:	NIASO_18765

NIA:	A8C56_16950

FLA:	SY85_05335

AGI:	FSB73_19460

ARAC:

E0W69_005870

FLN:

FLA_4835

PSEG:

D3H65_04885

PCM:	AY601_2523

PEK:	FFJ24_022710

PROE:

H9L23_23000

SHG:	Sph21_1452

MUP:	A0256_20255

MUC:

MuYL_1839

MGOT:

MgSA37_01958(csxA_3)

MUH:	HYN43_006850

MGIN:

FRZ54_16465

MGK:	FSB76_29410

MRUB:

DEO27_007190

MGOS:

DIU38_007020

MMAB:

HQ865_04360

OLI:	FKG96_04205

SBX:	CA265_21990

EVI:	Echvi_3868

EST:	DN752_02690

SPIK:

EXU85_03505

SRHO:

HH216_15960

HYZ:	AXW84_06640

HNV:	DDQ68_00540

RUD:	TH61_17245

NIB:	GU926_04950

ZPR:

ZPR_4166

SPON:

HME9304_00657(csxA)

» show all

Reference

1 [PMID:15247239]

Authors

Ishimizu T, Sasaki A, Okutani S, Maeda M, Yamagishi M, Hase S

Title

Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization.

Journal

J Biol Chem 279:38555-62 (2004)
DOI:10.1074/jbc.M406886200

Sequence

[ath:AT1G09010]

Reference

2 [PMID:9990135]

Authors

Sasaki A, Yamagishi M, Mega T, Norioka S, Natsuka S, Hase S.

Title

Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium longflorum thumb.

Journal

J Biochem (Tokyo) 125:363-7 (1999)
DOI:10.1093/oxfordjournals.jbchem.a022295

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.152

IUBMB Enzyme Nomenclature:

3.2.1.152

ExPASy - ENZYME nomenclature database:

3.2.1.152

BRENDA, the Enzyme Database:

3.2.1.152

CAS:	141176-95-6

All links

Gene (1330)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (262)   
   KEGG MGENES (969)   
   RefGene (98)   
Protein sequence (40)   
   UniProt (38)   
   SWISS-PROT (2)   
DNA sequence (32)   
   GenBank (16)   
   EMBL (16)   
Protein domain (11)   
   InterPro (11)   
All databases (1413)   

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