KEGG   ENZYME: 3.2.1.157Help
Entry
EC 3.2.1.157                Enzyme                                 

Name
iota-carrageenase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
iota-carrageenan 4-beta-D-glycanohydrolase (configuration-inverting)
Reaction(IUBMB)
Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose-2-sulfate in iota-carrageenans
Comment
The main products of hydrolysis are iota-neocarratetraose sulfate and iota-neocarrahexaose sulfate. iota-Neocarraoctaose is the shortest substrate oligomer that can be cleaved. Unlike EC 3.2.1.81, beta-agarase and EC 3.2.1.83, kappa-carrageenase, this enzyme proceeds with inversion of the anomeric configuration. iota-Carrageenan differs from kappa-carrageenan by possessing a sulfo group on O-2 of the 3,6-anhydro-D-galactose residues, in addition to that present in the kappa-compound on O-4 of the D-galactose residues.
History
EC 3.2.1.157 created 2006
Orthology
K20850  iota-carrageenase
Genes
CAO: Celal_3965
CLY: Celly_2877
CLH: IX49_14485
CBAL: M667_16305
CBAT: M666_16290
ZGA: ZOBELLIA_4265(cgiA1)
WFU: AXE80_00300
Taxonomy
Reference
1  [PMID:10934194]
  Authors
Barbeyron T, Michel G, Potin P, Henrissat B, Kloareg B.
  Title
iota-Carrageenases constitute a novel family of glycoside hydrolases, unrelated to that of kappa-carrageenases.
  Journal
J Biol Chem 275:35499-505 (2000)
DOI:10.1074/jbc.M003404200
  Sequence
Reference
2  [PMID:11493601]
  Authors
Michel G, Chantalat L, Fanchon E, Henrissat B, Kloareg B, Dideberg O.
  Title
The iota-carrageenase of Alteromonas fortis. A beta-helix fold-containing enzyme for the degradation of a highly polyanionic polysaccharide.
  Journal
J Biol Chem 276:40202-9 (2001)
DOI:10.1074/jbc.M100670200
  Sequence
Reference
3  [PMID:14623184]
  Authors
Michel G, Helbert W, Kahn R, Dideberg O, Kloareg B.
  Title
The structural bases of the processive degradation of iota-carrageenan, a main cell wall polysaccharide of red algae.
  Journal
J Mol Biol 334:421-33 (2003)
DOI:10.1016/j.jmb.2003.09.056
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.157
IUBMB Enzyme Nomenclature: 3.2.1.157
ExPASy - ENZYME nomenclature database: 3.2.1.157
BRENDA, the Enzyme Database: 3.2.1.157
CAS: 50936-37-3

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