Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy

agarose 3-glycanohydrolase

Endohydrolysis of (1->3)-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product

Requires Ca2+. The enzyme from Thalassomonas sp. can use agarose, agarohexaose and neoagarohexaose as substrate. The products of agarohexaose hydrolysis are dimers and tetramers, with agarotetraose being the predominant product, whereas hydrolysis of neoagarohexaose gives rise to two types of trimer. While the enzyme can also hydrolyse the highly sulfated agarose porphyran very efficiently, it cannot hydrolyse the related compounds kappa-carrageenan (see EC 3.2.1.83) and iota-carrageenan (see EC 3.2.1.157) [2]. See also EC 3.2.1.81, beta-agarase.

EC 3.2.1.158 created 2006

1  [PMID:8513809]

Potin P, Richard C, Rochas C, Kloareg B

Purification and characterization of the alpha-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B.

Eur J Biochem 214:599-607 (1993)
DOI:10.1111/j.1432-1033.1993.tb17959.x

[ag:AAF26838]

2  [PMID:15902469]

Ohta Y, Hatada Y, Miyazaki M, Nogi Y, Ito S, Horikoshi K

Purification and characterization of a novel alpha-agarase from a Thalassomonas sp.

Curr Microbiol 50:212-6 (2005)
DOI:10.1007/s00284-004-4435-z

[ag:BAF44076]