Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Hydrolysis of the (1->3)-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose
When neoagarohexaose is used as a substrate, the oligosaccharide is cleaved at the non-reducing end to produce 3,6-anhydro-L-galactose and agaropentaose, which is further hydrolysed to agarobiose and agarotriose. With neoagarotetraose as substrate, the products are predominantly agarotriose and 3,6-anhydro-L-galactose. In Vibrio sp. the actions of EC 184.108.40.206, beta-agarase and EC 220.127.116.11 can be used to degrade agarose to 3,6-anhydro-L-galactose and D-galactose.