KEGG   ENZYME: 3.2.1.165Help
Entry
EC 3.2.1.165                Enzyme                                 

Name
exo-1,4-beta-D-glucosaminidase;
CsxA;
GlcNase;
exochitosanase;
GlmA;
exo-beta-D-glucosaminidase;
chitosan exo-1,4-beta-D-glucosaminidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
chitosan exo-(1->4)-beta-D-glucosaminidase
Reaction(IUBMB)
Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini [RN:R06225 R08715]
Reaction(KEGG)
Comment
Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi and comprises D-glucosamine residues with a variable content of GlcNAc residues [4]. Acts specifically on chitooligosaccharides and chitosan, having maximal activity on chitotetraose, chitopentaose and their corresponding alcohols [1]. The enzyme can degrade GlcN-GlcNAc but not GlcNAc-GlcNAc [3]. A member of the glycoside hydrolase family 2 (GH-2) [4].
History
EC 3.2.1.165 created 2008
Pathway
ec00520  Amino sugar and nucleotide sugar metabolism
ec01100  Metabolic pathways
Orthology
K15855  exo-1,4-beta-D-glucosaminidase
Genes
ZJU: 107408972
NCR: NCU09042
NTE: NEUTE1DRAFT130945(NEUTE1DRAFT_130945)
SMP: SMAC_05835
PAN: PODANSg2615
TTT: THITE_2049779
MTM: MYCTH_54601
CTHR: CTHT_0049390
MGR: MGG_05864
SSCK: SPSK_06994
MAW: MAC_05140
MAJ: MAA_09634
CMT: CCM_04923
ANI: AN2824.2
ANG: ANI_1_2638014(An01g06630)
ABE: ARB_07888
TVE: TRV_00950
PTE: PTT_07728
SRT: Srot_2920
SMA: SAVERM_1223(csxA)
SBH: SBI_00935(bga4)
SHY: SHJG_2189
SRW: TUE45_00966(csxA_1)
SMAL: SMALA_7195
SLX: SLAV_03175(csxA)
AMD: AMED_6094
AMN: RAM_31240
AMM: AMES_6008
AMZ: B737_6008
AOI: AORI_5124
AJA: AJAP_13860(csxA)
SESP: BN6_82410(csxA)
CAI: Caci_7497
ACA: ACP_1263
ABAS: ACPOL_4897
SUS: Acid_7527
GBA: J421_6020
BCEL: BcellWH2_01738(csxA_2)
PSAC: PSM36_0032
PRU: PRU_0394
MUC: MuYL_2241
ZPR: ZPR_2497
AALG: AREALGSMS7_01260(csxA)
CABY: Cabys_1956
 » show all
Taxonomy
Reference
1  [PMID:2351651]
  Authors
Nanjo F, Katsumi R, Sakai K.
  Title
Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis.
  Journal
J Biol Chem 265:10088-94 (1990)
  Sequence
Reference
2  [PMID:16349528]
  Authors
Nogawa M, Takahashi H, Kashiwagi A, Ohshima K, Okada H, Morikawa Y.
  Title
Purification and Characterization of Exo-beta-d-Glucosaminidase from a Cellulolytic Fungus, Trichoderma reesei PC-3-7.
  Journal
Appl Environ Microbiol 64:890-895 (1998)
  Sequence
Reference
3  [PMID:16877749]
  Authors
Fukamizo T, Fleury A, Cote N, Mitsutomi M, Brzezinski R.
  Title
Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism.
  Journal
Glycobiology 16:1064-72 (2006)
DOI:10.1093/glycob/cwl026
Reference
4  [PMID:16316314]
  Authors
Cote N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R.
  Title
Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases.
  Journal
Biochem J 394:675-86 (2006)
DOI:10.1042/BJ20051436
  Sequence
Reference
5  [PMID:16636831]
  Authors
Ike M, Isami K, Tanabe Y, Nogawa M, Ogasawara W, Okada H, Morikawa Y.
  Title
Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7.
  Journal
Appl Microbiol Biotechnol 72:687-95 (2006)
DOI:10.1007/s00253-006-0320-y
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.165
IUBMB Enzyme Nomenclature: 3.2.1.165
ExPASy - ENZYME nomenclature database: 3.2.1.165
BRENDA, the Enzyme Database: 3.2.1.165

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