KEGG ENZYME: 3.2.1.165

ENZYME: 3.2.1.165

Entry

EC 3.2.1.165                Enzyme

Name

exo-1,4-beta-D-glucosaminidase;
CsxA;
GlcNase;
exochitosanase;
GlmA;
exo-beta-D-glucosaminidase;
chitosan exo-1,4-beta-D-glucosaminidase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

chitosan exo-(1->4)-beta-D-glucosaminidase

Reaction(IUBMB)

Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini [RN:R06225 R08715]

Reaction(KEGG)

R06225(G) R08715 > R01966 R06225(G)

Comment

Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi and comprises D-glucosamine residues with a variable content of GlcNAc residues [4]. Acts specifically on chitooligosaccharides and chitosan, having maximal activity on chitotetraose, chitopentaose and their corresponding alcohols [1]. The enzyme can degrade GlcN-GlcNAc but not GlcNAc-GlcNAc [3]. A member of the glycoside hydrolase family 2 (GH-2) [4].

History

EC 3.2.1.165 created 2008

Pathway

ec00520

Amino sugar and nucleotide sugar metabolism

ec01100

Metabolic pathways

Orthology

K15855

exo-1,4-beta-D-glucosaminidase

Genes

NCR:

NCU09042

NTE:	NEUTE1DRAFT130945(NEUTE1DRAFT_130945)

SMP:	10803036(SMAC4_05835)

PAN:	PODANSg2615

PBEL:

QC761_510470

PPSD:

QC762_510470

PPSP:

QC763_510470

PPSA:

QC764_510470

TTT:	THITE_2049779

MTM:	MYCTH_54601

CTHR:

CTHT_0049390

MGR:

MGG_05864

PPEI:

PpBr36_02687

PGRI:

PgNI_02262

SSCK:

SPSK_06994

FGR:	FGSG_02314

FPU:	FPSE_03809

FPOA:

FPOAC1_002486

FVN:	FVRRES_02707

FVR:	FVEG_17613

FMU:	J7337_012168

TRE:	TRIREDRAFT_77299

TRR:	M419DRAFT_24455

TATV:

25775408(TrAtP1_011908)

TASP:

36608107(GLS1)

MAW:	19249451(GLS93)

MAJ:

MAA_09634

MBRN:

26246956(gls93)

PCHM:

VFPPC_06548 VFPPC_06806 VFPPC_11872

CMT:

CCM_04923

PLJ:	28891694(PLICBS_010261) 28892851(PLICBS_009016)

PTKZ:

JDV02_002139 JDV02_005701(GLS93)

ELA:	UCREL1_5242

PFY:	PFICI_01243

ANI:	ANIA_02824

AFM:	AFUA_4G00390

ACT:	ACLA_078510

NFI:	NFIA_045250

AOR:	AO090003001410

ANG:	An01g06630

AFV:	AFLA_000268

ALUC:

AKAW2_20753S

APUU:

APUU_81022S

PCS:	N7525_001260

PDP:	PDIP_77980

POU:	POX_g09349

TRG:	TRUGW13939_11757

URE:	UREG_02485

ABE:

ARB_07888

TVE:

TRV_00950

PNO:	SNOG_15490

PTE:

PTT_07728

PTRR:

6349172(PtrM4_131400)

BZE:	COCCADRAFT_5109

BSC:	COCSADRAFT_136281

BOR:	COCMIDRAFT_25729

ARAB:

EKO05_0010065

PFJ:	MYCFIDRAFT_86684

TASA:

A1Q1_02923

PWI:	MWN52_01745

RGL:	CS053_09885

DJI:	CH75_09920

DTL:	H8F01_03210

DTA:	DYST_00557

DYG:	DYGSA30_29770

LPY:	FIV34_02685

LANH:

KR767_07885

LFL:	IM816_04405

LAEG:

L2Y94_01025

LAEO:

L2Y97_02650

LAES:

L2Y96_03010

AGE:	AA314_04805

NNV:	QNH39_10435

HPK:	Hprae_1313

MSA:	Mycsm_03422

MYV:	G155_16065

MYE:	AB431_16420

MDX:	BTO20_16235

MKU:	I2456_16720

MPAG:

C0J29_19915

MADI:

A7U43_15630

MCB:	Mycch_2879

MFT:	XA26_32930

MLL:	B1R94_13275

MMAG:

MMAD_29410

MFX:	MFAL_08710

MAIC:

MAIC_28430

MIJ:	MINS_27820

MALV:

MALV_07040

MPSC:

MPSYJ_35140

MARZ:

MARA_08700

MHEV:

MHEL_52080

MSAR:

MSAR_39990

MANY:

MANY_14110

MAUB:

MAUB_06510

MPHU:

MPHO_06090

MMUC:

C1S78_015285

MMAT:

MMAGJ_65440

MBOK:

MBOE_41550

MFG:	K6L26_15840

MSEI:

MSEDJ_43030

MGO:	AFA91_22240

MSEN:

K3U95_13525

MPAE:

K0O64_15515

MRF:	MJO55_14580

MCRO:

MI149_16475

MGRO:

FZ046_20445

SRT:

Srot_2920

SMA:	SAVERM_1223(csxA)

SVL:	Strvi_3876

SHY:

SHJG_2189

SHO:	SHJGH_1954

SRC:	M271_06075

SMAL:

SMALA_7195

SSOI:

I1A49_39755

SBH:	SBI_00935(bga4)

SCI:	B446_04595

SCX:	AS200_40475

SRW:	TUE45_00966(csxA_1)

SGS:	AVL59_27620

SCAD:

DN051_02655

SAUO:

BV401_39605

SPUN:

BFF78_38835

SLX:	SLAV_03175(csxA)

SRJ:

SRO_6680

SVN:	CP980_01980

SGF:	HEP81_07022

SSEO:

D0Z67_00815

SNF:	JYK04_00492(csxA)

SBRO:

GQF42_06070

SCHF:

IPT68_01635

SHUN:

DWB77_01272(csxA_2)

SCYG:

S1361_03420(csxA)

SDD:	D9753_32935

SYAN:

NRK68_01270

SSPN:

LXH13_02735

SXT:	KPP03845_100436(csxA)

SANT:

QR300_05110

SARG:

HKX69_31955

STTN:

BSL84_01920

SINN:

ABB07_34695

STPB:

QR97_39150

SMIB:

SMIR_37925

STRW:

QHG49_03395

STRL:

HEP84_07210

SRPZ:

HEP85_04285

STRH:

GXP74_11685

KQI:	F1D05_31905

ACIR:

OG979_21010

ACTQ:

OG417_35905

AMD:	AMED_3420 AMED_5067 AMED_6094

AMN:	RAM_17400 RAM_25805 RAM_31240

AMM:	AMES_3381 AMES_5007 AMES_6008

AMZ:	B737_3381 B737_5007 B737_6008

AOI:

AORI_5124

AJA:	AJAP_13860(csxA)

AMYC:

CU254_13085 CU254_22695

AMYB:

BKN51_18055

AMYY:

YIM_31765(csxA2)

AORI:

SD37_25235

AACD:

LWP59_02905

APRT:

MUY14_05400 MUY14_18295

AROO:

NQK81_41205

ARHD:

VSH64_04020

AMOG:

QRX60_34480 QRX60_47310

ACOR:

LCL61_20370

AMYZ:

HUW46_08023(csxA)

SESP:

BN6_82410(csxA)

KAL:	KALB_8547 KALB_8660

KUT:	JJ691_11740 JJ691_80470

KPHY:

AOZ06_13880 AOZ06_38310 AOZ06_47505

LED:	BBK82_15950

CROS:

N8J89_40265 N8J89_40270

UME:	RM788_51450

MTEM:

GCE86_09470

DFU:	Dfulv_32370

DACT:

KZZ52_44305

CAI:

Caci_7497

OCK:	EXM22_08615

ABA:	Acid345_1359

ACA:

ACP_1263

TRS:	Terro_3039

ABAS:

ACPOL_4897

ADIN:

H7849_25270

TGI:	RBB81_18030

TPSC:

RBB77_23220

SUS:

Acid_7527

PFER:

IRI77_05355

GEP:	Q9293_09020 Q9293_14945

GBA:

J421_6020

BCEL:

BcellWH2_01738(csxA_2)

PSAC:

PSM36_0032

PRU:

PRU_0394

MCOS:

GM418_25040

ASX:	CDL62_00075

CBAE:

COR50_19985

AGI:	FSB73_19960

ATEE:

K9M52_18285

PGIN:

FRZ67_21820

THEO:

IMW88_10245

SHG:	Sph21_1908

MUC:

MuYL_2241

MUH:	HYN43_011735

MGIN:

FRZ54_02725

MRUB:

DEO27_012770

MSAB:

SNE25_22520

OLI:	FKG96_06465

EMZ:	MB380_16075

ALS:	DJ013_10140

RSI:	Runsl_2770

RUN:	DR864_11915

FCG:	LNP23_06810

ZPR:

ZPR_2497

AALG:

AREALGSMS7_01260(csxA)

EMAR:

D1013_15210

SHYR:

LA303_00705

CABY:

Cabys_1956

LOB:	NEF87_002321

» show all

Reference

1 [PMID:2351651]

Authors

Nanjo F, Katsumi R, Sakai K.

Title

Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis.

Journal

J Biol Chem 265:10088-94 (1990)

Sequence

[ag:AAX62629]

Reference

2 [PMID:16349528]

Authors

Nogawa M, Takahashi H, Kashiwagi A, Ohshima K, Okada H, Morikawa Y.

Title

Purification and Characterization of Exo-beta-d-Glucosaminidase from a Cellulolytic Fungus, Trichoderma reesei PC-3-7.

Journal

Appl Environ Microbiol 64:890-895 (1998)
DOI:10.1128/AEM.64.3.890-895.1998

Sequence

[ag:BAD99604]

Reference

3 [PMID:16877749]

Authors

Fukamizo T, Fleury A, Cote N, Mitsutomi M, Brzezinski R.

Title

Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism.

Journal

Glycobiology 16:1064-72 (2006)
DOI:10.1093/glycob/cwl026

Reference

4 [PMID:16316314]

Authors

Cote N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R

Title

Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases.

Journal

Biochem J 394:675-86 (2006)
DOI:10.1042/BJ20051436

Sequence

[ag:AAX62629]

Reference

5 [PMID:16636831]

Authors

Ike M, Isami K, Tanabe Y, Nogawa M, Ogasawara W, Okada H, Morikawa Y

Title

Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7.

Journal

Appl Microbiol Biotechnol 72:687-95 (2006)
DOI:10.1007/s00253-006-0320-y

Sequence

[ag:BAD99604]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.165

IUBMB Enzyme Nomenclature:

3.2.1.165

ExPASy - ENZYME nomenclature database:

3.2.1.165

BRENDA, the Enzyme Database:

3.2.1.165

DBGET integrated database retrieval system