| Entry |
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| Name |
heparanase;
Hpa1 heparanase;
Hpa1;
heparanase 1;
heparanase-1;
C1A heparanase;
HPSE
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| Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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| Sysname |
heparan sulfate N-sulfo-D-glucosamine endoglucanase
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| Reaction(IUBMB) |
endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan
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| Reaction(KEGG) |
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| Comment |
Heparanase cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group [2]. Heparanase-1 cuts macromolecular heparin into fragments of 5000--20000 Da [5]. The enzyme cleaves the heparan sulfate glycosaminoglycans from proteoglycan core proteins and degrades them to small oligosaccharides. Inside cells, the enzyme is important for the normal catabolism of heparan sulfate proteoglycans, generating glycosaminoglycan fragments that are then transported to lysosomes and completely degraded. When secreted, heparanase degrades basement membrane heparan sulfate glycosaminoglycans at sites of injury or inflammation, allowing extravasion of immune cells into nonvascular spaces and releasing factors that regulate cell proliferation and angiogenesis [1].
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| History |
EC 3.2.1.166 created 2010
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| Pathway |
| ec00531 | Glycosaminoglycan degradation |
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| Orthology |
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| Genes |
» show all
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| Reference |
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| Authors |
Bame KJ |
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| Title |
Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans. |
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| Journal |
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| Reference |
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| Authors |
Peterson SB, Liu J |
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| Title |
Unraveling the specificity of heparanase utilizing synthetic substrates. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Pikas DS, Li JP, Vlodavsky I, Lindahl U |
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| Title |
Substrate specificity of heparanases from human hepatoma and platelets. |
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| Journal |
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| Reference |
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| Authors |
Okada Y, Yamada S, Toyoshima M, Dong J, Nakajima M, Sugahara K |
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| Title |
Structural recognition by recombinant human heparanase that plays critical roles in tumor metastasis. Hierarchical sulfate groups with different effects and the essential target disulfated trisaccharide sequence. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Vreys V, David G |
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| Title |
Mammalian heparanase: what is the message? |
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| Journal |
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| Reference |
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| Authors |
Gong F, Jemth P, Escobar Galvis ML, Vlodavsky I, Horner A, Lindahl U, Li JP |
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| Title |
Processing of macromolecular heparin by heparanase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Toyoshima M, Nakajima M |
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| Title |
Human heparanase. Purification, characterization, cloning, and expression. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Miao HQ, Navarro E, Patel S, Sargent D, Koo H, Wan H, Plata A, Zhou Q, Ludwig D, Bohlen P, Kussie P |
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| Title |
Cloning, expression, and purification of mouse heparanase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Hammond E, Li CP, Ferro V |
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| Title |
Development of a colorimetric assay for heparanase activity suitable for kinetic analysis and inhibitor screening. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 3.2.1.166 |
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