KEGG   ENZYME: 3.2.1.169
Entry
EC 3.2.1.169                Enzyme                                 

Name
protein O-GlcNAcase;
OGA;
glycoside hydrolase O-GlcNAcase;
O-GlcNAcase;
BtGH84;
O-GlcNAc hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase
Reaction(IUBMB)
(1) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine  [RN:R09672];
(2) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-theronine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine [RN:R09673]
Reaction(KEGG)
R09672 R09673
Substrate
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine [CPD:C19802];
H2O [CPD:C00001];
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-theronine
Product
[protein]-L-serine [CPD:C02189];
N-acetyl-D-glucosamine [CPD:C00140];
[protein]-L-threonine [CPD:C19803]
Comment
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
History
EC 3.2.1.169 created 2011
Orthology
K15719  protein O-GlcNAcase / histone acetyltransferase
Genes
HSA: 10724(OGA)
PTR: 450691(MGEA5)
PPS: 100979884(OGA)
GGO: 101124144(OGA)
PON: 100459796(MGEA5)
NLE: 100586192(OGA)
MCC: 712370(OGA)
MCF: 102142813(MGEA5)
CSAB: 103216418(MGEA5)
CATY: 105581866(MGEA5)
PANU: 101015029(OGA)
RRO: 104656503(OGA)
RBB: 108515105(MGEA5)
TFN: 117067826(OGA)
PTEH: 111538829(OGA)
CJC: 100392114(MGEA5)
SBQ: 101029093(MGEA5)
MMUR: 105874010(MGEA5)
MMU: 76055(Oga)
MCAL: 110285308(Mgea5)
MPAH: 110313432(Mgea5)
RNO: 154968(Oga)
MCOC: 116103925(Oga)
MUN: 110556269(Mgea5)
CGE: 100751586(Oga)
PLEU: 114708145(Oga)
NGI: 103744007(Mgea5)
HGL: 101702515(Mgea5)
CPOC: 100721083(Mgea5)
CCAN: 109691315(Mgea5)
OCU: 100351779(MGEA5)
OPI: 101534359(OGA)
TUP: 102491291(MGEA5)
CFA: 477802(OGA)
VVP: 112915175(OGA)
VLG: 121484059(OGA)
AML: 100472078(OGA)
UMR: 103679457(MGEA5)
UAH: 113251855(OGA)
ORO: 101374982(MGEA5)
ELK: 111144756
MPUF: 101688735(MGEA5)
EJU: 114205483(OGA)
MLX: 118025273(OGA)
FCA: 101098493(MGEA5)
PYU: 121037144(OGA)
PBG: 122493164(OGA)
PTG: 102959286(MGEA5)
PPAD: 109272801(MGEA5)
AJU: 106966947(OGA)
HHV: 120239568(OGA)
BTA: 538561(OGA)
BOM: 102279409(MGEA5)
BIU: 109578999(MGEA5)
BBUB: 102398663(OGA)
CHX: 102179540(MGEA5)
OAS: 101113978(OGA)
ODA: 120873553(OGA)
CCAD: 122445997(OGA)
SSC: 100152585(MGEA5)
CFR: 102507074(OGA)
CBAI: 105081720(MGEA5)
CDK: 105084341(OGA)
BACU: 103001043(MGEA5)
LVE: 103070168(MGEA5)
OOR: 101288973(MGEA5)
DLE: 111172372(OGA)
PCAD: 102973862(OGA)
PSIU: 116741261(OGA)
ECB: 100060245(MGEA5)
EPZ: 103551392(MGEA5)
EAI: 106828857(OGA)
MYB: 102258473(MGEA5)
MYD: 102763853(MGEA5)
MMYO: 118669820(OGA)
MLF: 102432555(MGEA5)
MNA: 107542582(MGEA5)
PKL: 118718900(OGA)
HAI: 109379646(MGEA5)
DRO: 112300370(MGEA5)
SHON: 118999490(OGA)
AJM: 119059637(OGA)
PDIC: 114496600(OGA)
MMF: 118632007(OGA)
RFQ: 117035786(OGA)
PALE: 102892734(OGA)
PGIG: 120609167(OGA)
RAY: 107497783(MGEA5)
MJV: 108405927(MGEA5)
TOD: 119233350(OGA)
LAV: 100660277(MGEA5)
TMU: 101348318
MDO: 100023348(MGEA5)
GAS: 123238135(OGA)
SHR: 100924785(OGA)
PCW: 110194632(MGEA5)
OAA: 100086916(OGA)
GGA: 423851(OGA) 430422
PCOC: 116226056 116231682(OGA)
MGP: 100550244(OGA) 116216702
CJO: 107306849 107315982(OGA)
NMEL: 110390931 110399611(MGEA5)
APLA: 101798266(OGA)
ACYG: 106035955(MGEA5) 106043195
TGU: 100217691 100222791(OGA)
LSR: 110468741 110473663(OGA)
SCAN: 103814148(OGA) 103823117
PMOA: 120502106(OGA) 120509741
OTC: 121335221 121341962(OGA)
PRUF: 121350033(OGA) 121364720
GFR: 102039689(MGEA5) 102041996
FAB: 101807026(MGEA5) 101820674
PHI: 102099607 102100524(MGEA5)
PMAJ: 107203361 107206955(MGEA5)
CCAE: 111929151 111930940(MGEA5)
CCW: 104690840 104697891(OGA)
ETL: 114068881 114070525(OGA)
FPG: 101911645 101920070(MGEA5)
FCH: 102050212 102050673(OGA)
CLV: 102086261 102092419(MGEA5)
EGZ: 104125639(MGEA5)
NNI: 104013575(MGEA5)
ACUN: 113481496(OGA) 113490509
PADL: 103916317(MGEA5) 103922179
AAM: 106487392 106496884(MGEA5)
AROW: 112967879(OGA) 112978201
NPD: 112957020(OGA)
DNE: 112984773(OGA)
ASN: 102378650 102387046(OGA)
AMJ: 102563356(MGEA5) 106740206
CPOO: 109323917(MGEA5) 109324416
GGN: 109287545(MGEA5)
PSS: 102451920(OGA)
CMY: 102948301(OGA)
CPIC: 101934313(OGA)
TST: 117879788(OGA)
CABI: 116833940(OGA)
MRV: 120409101(OGA)
ACS: 100559082(mgea5)
PVT: 110077588(MGEA5)
SUND: 121927346(OGA)
PBI: 103061735(OGA)
PMUR: 107285267(OGA)
TSR: 106549157(MGEA5)
PGUT: 117678619(OGA)
VKO: 123026895(OGA)
PMUA: 114597566(OGA)
ZVI: 118093544(OGA)
GJA: 107114456(MGEA5)
XLA: 108696157(oga.L) 108697432(oga.S)
XTR: 100145437(oga)
NPR: 108784409(MGEA5)
DRE: 100538182(si:dkey-183c6.8) 324487(oga) 571547(mgea5l)
CCAR: 109069987 109083210(mgea5)
LCO: 104922274(oga) 104925209
ELY: 117248314(oga) 117255350(ogal) 117272795(si:dkey-183c6.8)
PLEP: 121941846(ogal) 121954223(oga) 121962152(si:dkey-183c6.8)
SLUC: 116042246(si:dkey-183c6.8) 116042595(ogal) 116053576(oga)
ECRA: 117934563(si:dkey-183c6.8) 117936653(ogal) 117960762(oga)
GAT: 120820615(oga) 120822207(si:dkey-183c6.8) 120825243(ogal)
PPUG: 119214134(oga) 119218258(ogal) 119222545(si:dkey-183c6.8)
MSAM: 119899547(ogal) 119912245 119917666(oga)
CUD: 121504818(si:dkey-183c6.8) 121508370(ogal) 121511590(oga)
OAU: 116319631(oga) 116322277(si:dkey-183c6.8) 116330977(ogal)
OML: 112137423(ogal) 112155772(si:dkey-183c6.8) 112161614(oga)
GAF: 122820190(si:dkey-183c6.8) 122829586(ogal) 122842221(oga)
CTUL: 119772716(si:dkey-183c6.8) 119783282(ogal) 119790729(oga)
KMR: 108234080(oga) 108236421(ogal) 108242892(si:dkey-183c6.8)
CSEM: 103383859 103387380(mgea5)
SSEN: 122766735(si:dkey-183c6.8) 122771484(ogal) 122781668(oga)
HHIP: 117751790(si:dkey-183c6.8) 117767478(ogal) 117775389(oga)
XGL: 120796260(oga) 120797482(si:dkey-183c6.8) 120800240(ogal)
OTW: 112214489(si:dkey-183c6.8) 112242091(ogal) 112252425(oga) 112252769
OMY: 110485509(ogal) 110493422 110500330(si:dkey-183c6.8) 110502247(oga) 110526041
SNH: 120017500 120018110 120041436 120046445(oga) 120049052(si:dkey-183c6.8)
SFM: 108920620 108930276(oga)
AANG: 118217811(oga) 118218961 118231584(si:dkey-183c6.8)
LOC: 102683302 102694479(mgea5)
PSPA: 121294585(si:dkey-183c6.8) 121321684 121325549
ARUT: 117409458(si:dkey-183c6.8) 117415414 117418184
LCM: 102346400(MGEA5) 102358152
CMK: 103190567(oga)
RTP: 109923289(mgea5) 109924748
BFO: 118413805
BBEL: 109471441
CIN: 100179919
SCLV: 120338667
SPU: 585537
APLC: 110981261
SKO: 100378403
DME: Dmel_CG5871(Oga)
DER: 6553422
DSE: 6620036
DSI: Dsimw501_GD19407(Dsim_GD19407)
DAN: 6494447
DSR: 110188182
DPE: 6598788
DMN: 108154237
DWI: 6647454
DGR: 6567353
DAZ: 108608693
DNV: 108652629
DHE: 111602671
DVI: 6634891
CCAT: 101450686
BOD: 106617257
MDE: 101893356
SCAC: 106084367
LCQ: 111688191
ACOZ: 120951771
AARA: 120908619
AAG: 5571796
CPII: 120416025
AME: 411905
ACER: 108000941
BIM: 100749208
BBIF: 117215870
BVK: 117238281
BVAN: 117160087
BTER: 100645450
BPYO: 122569957
CCAL: 108624654
OBB: 114878167
MGEN: 117224355
NMEA: 116429460
CGIG: 122396249
SOC: 105196907
MPHA: 105835235
AEC: 105154917
ACEP: 105618479
PBAR: 105430117
VEM: 105563901
HST: 105189349
DQU: 106747473
CFO: 105248789
FEX: 115237312
LHU: 105675868
PGC: 109860249
OBO: 105281645
PCF: 106793487
PFUC: 122516433
VPS: 122628814
NVI: 100119788
CSOL: 105360693
TPRE: 106652648
MDL: 103571170
CGLO: 123263502
FAS: 105270416
DAM: 107038060
AGIF: 122851854
CCIN: 107267294
TCA: 655306
DPA: 109539113
ATD: 109597139
AGB: 108904637
LDC: 111511864
NVL: 108558025
APLN: 108744311
PPYR: 116175725
OTU: 111423601
BMOR: 101735953
BMAN: 114253038
MSEX: 115451650
BANY: 112055407
PMAC: 106719051
PPOT: 106102929
PXU: 106116405
PRAP: 110998763
ZCE: 119837823
HAW: 110383470
TNL: 113507217
API: 100163269
DNX: 107165302
AGS: 114131204
RMD: 113554292
BTAB: 109033140
CLEC: 106662476
HHAL: 106678327
FOC: 113207022
ZNE: 110832426
CSEC: 111873810
FCD: 110863215
PJA: 122252320
HAME: 121864984
HAZT: 108679419
EAF: 111700900
DSV: 119459137
RSAN: 119379212
RMP: 119175846
VDE: 111254346
VJA: 111270765
TUT: 107360407
DPTE: 113788567
PTEP: 107442614
SDM: 118189878
BMY: BM_BM5855(Bma-oga-1)
BGT: 106065918
GAE: 121384746
CRG: 105347264
MYI: 110449102
PMAX: 117339865
OBI: 106884279
OSN: 115218926
LAK: 106176558
EGL: EGR_04224
NVE: 5519790
EPA: 110245582
ATEN: 116292862
ADF: 107327833
AMIL: 114973993
PDAM: 113667448
SPIS: 111320587
DGT: 114515726
HMG: 100215737
AQU: 100640131
 » show all
Reference
1  [PMID:11148210]
  Authors
Gao Y, Wells L, Comer FI, Parker GJ, Hart GW
  Title
Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human  brain.
  Journal
J Biol Chem 276:9838-45 (2001)
DOI:10.1074/jbc.M010420200
  Sequence
[hsa:10724]
Reference
2  [PMID:11788610]
  Authors
Wells L, Gao Y, Mahoney JA, Vosseller K, Chen C, Rosen A, Hart GW
  Title
Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase.
  Journal
J Biol Chem 277:1755-61 (2002)
DOI:10.1074/jbc.m109656200
  Sequence
[hsa:10724]
Reference
3  [PMID:16533067]
  Authors
Cetinbas N, Macauley MS, Stubbs KA, Drapala R, Vocadlo DJ
  Title
Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants.
  Journal
Biochemistry 45:3835-44 (2006)
DOI:10.1021/bi052370b
Reference
4  [PMID:16565725]
  Authors
Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GN, Vocadlo DJ, Davies GJ
  Title
Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity.
  Journal
Nat Struct Mol Biol 13:365-71 (2006)
DOI:10.1038/nsmb1079
  Sequence
[bth:BT_4395]
Reference
5  [PMID:16584714]
  Authors
Kim EJ, Kang DO, Love DC, Hanover JA
  Title
Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate.
  Journal
Carbohydr Res 341:971-82 (2006)
DOI:10.1016/j.carres.2006.03.004
Reference
6  [PMID:8034696]
  Authors
Dong DL, Hart GW
  Title
Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol.
  Journal
J Biol Chem 269:19321-30 (1994)
  Sequence
[rno:154968]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.169
IUBMB Enzyme Nomenclature: 3.2.1.169
ExPASy - ENZYME nomenclature database: 3.2.1.169
BRENDA, the Enzyme Database: 3.2.1.169

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