Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy

[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase

(1) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine  [RN:R09672];
(2) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-theronine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine [RN:R09673]

R09672 R09673
Reaction

[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine [CPD:C19802];
H2O [CPD:C00001];
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-theronine

Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.

EC 3.2.1.169 created 2011

1  [PMID:11148210]

Gao Y, Wells L, Comer FI, Parker GJ, Hart GW

Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human  brain.

J Biol Chem 276:9838-45 (2001)
DOI:10.1074/jbc.M010420200

[hsa:10724]

2  [PMID:11788610]

Wells L, Gao Y, Mahoney JA, Vosseller K, Chen C, Rosen A, Hart GW

Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase.

J Biol Chem 277:1755-61 (2002)
DOI:10.1074/jbc.m109656200

[hsa:10724]

3  [PMID:16533067]

Cetinbas N, Macauley MS, Stubbs KA, Drapala R, Vocadlo DJ

Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants.

Biochemistry 45:3835-44 (2006)
DOI:10.1021/bi052370b

4  [PMID:16565725]

Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GN, Vocadlo DJ, Davies GJ

Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity.

Nat Struct Mol Biol 13:365-71 (2006)
DOI:10.1038/nsmb1079

[bth:BT_4395]

5  [PMID:16584714]

Kim EJ, Kang DO, Love DC, Hanover JA

Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate.

Carbohydr Res 341:971-82 (2006)
DOI:10.1016/j.carres.2006.03.004

6  [PMID:8034696]

Dong DL, Hart GW

Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol.

J Biol Chem 269:19321-30 (1994)

[rno:154968]