| Entry |
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| Name |
alpha-D-xyloside xylohydrolase;
alpha-xylosidase
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| Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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| Sysname |
alpha-D-xyloside xylohydrolase
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| Reaction(IUBMB) |
Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.
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| Comment |
The enzyme catalyses hydrolysis of a terminal, unsubstituted xyloside at the extreme reducing end of a xylogluco-oligosaccharide. Representative alpha-xylosidases from glycoside hydrolase family 31 utilize a two-step (double-displacement) mechanism involving a covalent glycosyl-enzyme intermediate, and retain the anomeric configuration of the product.
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| History |
EC 3.2.1.177 created 2011
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| Orthology |
| K01811 | alpha-D-xyloside xylohydrolase |
| K15925 | alpha-D-xyloside xylohydrolase |
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| Genes |
» show all
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| Reference |
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| Authors |
Moracci M, Cobucci Ponzano B, Trincone A, Fusco S, De Rosa M, van Der Oost J, Sensen CW, Charlebois RL, Rossi M |
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| Title |
Identification and molecular characterization of the first alpha -xylosidase from an archaeon. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Sampedro J, Sieiro C, Revilla G, Gonzalez-Villa T, Zarra I |
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| Title |
Cloning and expression pattern of a gene encoding an alpha-xylosidase active against xyloglucan oligosaccharides from Arabidopsis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Crombie HJ, Chengappa S, Jarman C, Sidebottom C, Reid JS |
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| Title |
Molecular characterisation of a xyloglucan oligosaccharide-acting alpha-D-xylosidase from nasturtium (Tropaeolum majus L.) cotyledons that resembles plant 'apoplastic' alpha-D-glucosidases. |
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| Journal |
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| Reference |
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| Authors |
Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC |
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| Title |
Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Iglesias N, Abelenda JA, Rodino M, Sampedro J, Revilla G, Zarra I |
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| Title |
Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Okuyama M, Kaneko A, Mori H, Chiba S, Kimura A |
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| Title |
Structural elements to convert Escherichia coli alpha-xylosidase (YicI) into alpha-glucosidase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Larsbrink J, Izumi A, Ibatullin FM, Nakhai A, Gilbert HJ, Davies GJ, Brumer H |
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| Title |
Structural and enzymatic characterization of a glycoside hydrolase family 31 alpha-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 3.2.1.177 |
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