KEGG   ENZYME: 3.2.1.189
Entry
EC 3.2.1.189                Enzyme                                 

Name
dioscin glycosidase (diosgenin-forming);
dioscin glycosidase (aglycone-forming)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin hydrolase (diosgenin-forming)
Reaction(IUBMB)
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O = D-glucose + 2 L-rhamnose + diosgenin [RN:R10581]
Reaction(KEGG)
R10581
Substrate
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin [CPD:C08897];
H2O [CPD:C00001]
Product
D-glucose [CPD:C00031];
L-rhamnose [CPD:C00507];
diosgenin [CPD:C08898]
Comment
The enzyme is involved in degradation of the steroid saponin dioscin by some fungi of the Absidia genus. The enzyme can also hydrolyse 3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin into diosgenin and free sugars as the final products. cf. EC 3.2.1.190, dioscin glycosidase (3-O-beta-D-Glc-diosgenin-forming).
History
EC 3.2.1.189 created 2013
Reference
1  [PMID:20622501]
  Authors
Fu Y, Yu H, Tang SH, Hu X, Wang Y, Liu B, Yu C, Jin F
  Title
New dioscin-glycosidase hydrolyzing multi-glycosides of dioscin from Absidia strain.
  Journal
J Microbiol Biotechnol 20:1011-7 (2010)
DOI:10.4014/jmb.0910.10039
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.189
IUBMB Enzyme Nomenclature: 3.2.1.189
ExPASy - ENZYME nomenclature database: 3.2.1.189
BRENDA, the Enzyme Database: 3.2.1.189

  All links  
Chemical substance (5)   
   KEGG COMPOUND (5)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
All databases (8)   

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