Entry |
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Name |
ginsenoside Rb1 beta-glucosidase
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Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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Sysname |
ginsenoside Rb1 glucohydrolase
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Reaction(IUBMB) |
ginsenoside Rb1 + 2 H2O = ginsenoside Rg3 + 2 D-glucopyranose (overall reaction);
(1a) ginsenoside Rb1 + H2O = ginsenoside Rd + D-glucopyranose;
(1b) ginsenoside Rd + H2O = ginsenoside Rg3 + D-glucopyranose
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Substrate |
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Product |
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Comment |
Ginsenosidases catalyse the hydrolysis of glycosyl moieties attached to the C-3, C-6 or C-20 position of ginsenosides. They are specific with respect to the nature of the glycosidic linkage, the position and the order in which the linkages are cleaved. Ginsenoside Rb1 beta-glucosidase specifically and sequentially hydrolyses the 20-[beta-D-glucopyranosyl-(1->6)-beta-D glucopyranosyloxy] residues attached to position 20 by first hydrolysing the (1->6)-glucosidic bond to generate ginsenoside Rd as an intermediate, followed by hydrolysis of the remaining 20-O-beta-D-glucosidic bond.
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History |
EC 3.2.1.192 created 2014
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Reference |
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Authors |
Yan Q, Zhou W, Li X, Feng M, Zhou P |
Title |
Purification method improvement and characterization of a novel ginsenoside-hydrolyzing beta-glucosidase from Paecilomyces Bainier sp. 229. |
Journal |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 3.2.1.192 |
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