Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

ginsenoside Rb1 glucohydrolase

ginsenoside Rb1 + 2 H2O = ginsenoside Rg3 + 2 D-glucopyranose (overall reaction);
(1a) ginsenoside Rb1 + H2O = ginsenoside Rd + D-glucopyranose;
(1b) ginsenoside Rd + H2O = ginsenoside Rg3 + D-glucopyranose

ginsenoside Rb1 [CPD:C20713];
H2O [CPD:C00001];
ginsenoside Rd [CPD:C20725]

Ginsenosidases catalyse the hydrolysis of glycosyl moieties attached to the C-3, C-6 or C-20 position of ginsenosides. They are specific with respect to the nature of the glycosidic linkage, the position and the order in which the linkages are cleaved. Ginsenoside Rb1 beta-glucosidase specifically and sequentially hydrolyses the 20-[beta-D-glucopyranosyl-(1->6)-beta-D glucopyranosyloxy] residues attached to position 20 by first hydrolysing the (1->6)-glucosidic bond to generate ginsenoside Rd as an intermediate, followed by hydrolysis of the remaining 20-O-beta-D-glucosidic bond.

EC 3.2.1.192 created 2014

1  [PMID:18256474]

Yan Q, Zhou W, Li X, Feng M, Zhou P

Purification method improvement and characterization of a novel ginsenoside-hydrolyzing beta-glucosidase from Paecilomyces Bainier sp. 229.

Biosci Biotechnol Biochem 72:352-9 (2008)
DOI:10.1271/bbb.70425