Entry |
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Name |
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Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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Sysname |
ginsenoside glucohydrolase
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Reaction(IUBMB) |
(1) a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O = a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose;
(2) a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + H2O = a protopanaxadiol-type ginsenoside with no glycosidic modifications at position 3 + D-glucopyranose;
(3) a protopanaxadiol-type ginsenoside with two glycosyl residues at position 20 + H2O = a protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20 + a monosaccharide
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Substrate |
protopanaxadiol-type ginsenoside with two glucosyl residues at position 3;
H2O [CPD: C00001];
protopanaxadiol-type ginsenoside with one glucosyl residue at position 3;
protopanaxadiol-type ginsenoside with two glycosyl residues at position 20
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Product |
protopanaxadiol-type ginsenoside with one glucosyl residue at position 3;
D-glucopyranose [CPD: C00031];
protopanaxadiol-type ginsenoside with no glycosidic modifications at position 3;
protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20;
monosaccharide
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Comment |
Ginsenosidase type I is slightly activated by Mg2+ or Ca2+ [1]. The enzyme hydrolyses the 3-O-beta-D-(1->2)-glucosidic bond, the 3-O-beta-D-glucopyranosyl bond and the 20-O-beta-D-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. It usually leaves a single glucosyl residue attached at position 20 and one or no glucosyl residues at position 3. Starting with a ginsenoside that is glycosylated at both positions (e.g. ginsenoside Rb1, Rb2, Rb3, Rc or Rd), the most common products are ginsenoside F2 and ginsenoside C-K, with low amounts of ginsenoside Rh2.
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History |
EC 3.2.1.193 created 2014
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Reference |
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Authors |
Yu H, Zhang C, Lu M, Sun F, Fu Y, Jin F |
Title |
Purification and characterization of new special ginsenosidase hydrolyzing multi-glycisides of protopanaxadiol ginsenosides, ginsenosidase type I. |
Journal |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 3.2.1.193 |
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