Entry |
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Name |
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Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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Sysname |
protopanaxatriol-type ginsenoside 6-beta-D-glucohydrolase
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Reaction(IUBMB) |
a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + 2 H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose + a monosaccharide (overall reaction);
(1a) a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + H2O = a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + a monosaccharide;
(1b) a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose
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Substrate |
protopanaxatriol-type ginsenoside with two glycosyl residues at position 6;
H2O [CPD: C00001];
protopanaxatriol-type ginsenoside with a single glucosyl at position 6
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Product |
protopanaxatriol-type ginsenoside with no glycosidic modification at position 6;
D-glucopyranose [CPD: C00031];
monosaccharide;
protopanaxatriol-type ginsenoside with a single glucosyl at position 6
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Comment |
Ginsenosidase type IV catalyses the sequential hydrolysis of the 6-O-beta-D-(1->2)-glycosidic bond or the 6-O-alpha-D-(1->2)-glycosidic bond in protopanaxatriol-type ginsenosides with a disacchride attached to the C6 position, followed by the hydrolysis of the remaining 6-O-beta-D-glycosidic bond (e.g. ginsenoside Re -> ginsenoside Rg1 -> ginsenoside F1).
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History |
EC 3.2.1.194 created 2014
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Reference |
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Authors |
Wang DM, Yu HS, Song JG, Xu YF, Liu CY, Jin FX |
Title |
A novel ginsenosidase from an Aspergillus strain hydrolyzing 6-O-multi-glycosides of protopanaxatriol-type ginsenosides, named ginsenosidase type IV. |
Journal |
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Reference |
2 |
Authors |
Wang, D, Yu, H., Song, J., Xu, Y., Jin, F. |
Title |
Enzyme kinetics of ginsenosidase type IV hydrolyzing 6-O-multi-glycosides of protopanaxatriol type ginsenosides. |
Journal |
Process Biochem 47:133-138 (2012) |
Other DBs |
ExPASy - ENZYME nomenclature database: | 3.2.1.194 |
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