Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

protopanaxatriol-type ginsenoside 6-beta-D-glucohydrolase

a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + 2 H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose + a monosaccharide (overall reaction);
(1a) a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + H2O = a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + a monosaccharide;
(1b) a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose

protopanaxatriol-type ginsenoside with two glycosyl residues at position 6;
H2O [CPD:C00001];
protopanaxatriol-type ginsenoside with a single glucosyl at position 6

Ginsenosidase type IV catalyses the sequential hydrolysis of the 6-O-beta-D-(1->2)-glycosidic bond or the 6-O-alpha-D-(1->2)-glycosidic bond in protopanaxatriol-type ginsenosides with a disacchride attached to the C6 position, followed by the hydrolysis of the remaining 6-O-beta-D-glycosidic bond (e.g. ginsenoside Re -> ginsenoside Rg1 -> ginsenoside F1).

EC 3.2.1.194 created 2014

1  [PMID:22031031]

Wang DM, Yu HS, Song JG, Xu YF, Liu CY, Jin FX

A novel ginsenosidase from an Aspergillus strain hydrolyzing 6-O-multi-glycosides of protopanaxatriol-type ginsenosides, named ginsenosidase type IV.

J Microbiol Biotechnol 21:1057-63 (2011)

2

Wang, D, Yu, H., Song, J., Xu, Y., Jin, F.

Enzyme kinetics of ginsenosidase type IV hydrolyzing 6-O-multi-glycosides of protopanaxatriol type ginsenosides.

Process Biochem 47:133-138 (2012)