a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + 2 H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose + a monosaccharide (overall reaction);
(1a) a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + H2O = a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + a monosaccharide;
(1b) a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose
Substrate
protopanaxatriol-type ginsenoside with two glycosyl residues at position 6;
H2O [CPD:C00001];
protopanaxatriol-type ginsenoside with a single glucosyl at position 6
Product
protopanaxatriol-type ginsenoside with no glycosidic modification at position 6;
D-glucopyranose [CPD:C00031];
monosaccharide;
protopanaxatriol-type ginsenoside with a single glucosyl at position 6
Comment
Ginsenosidase type IV catalyses the sequential hydrolysis of the 6-O-beta-D-(1->2)-glycosidic bond or the 6-O-alpha-D-(1->2)-glycosidic bond in protopanaxatriol-type ginsenosides with a disacchride attached to the C6 position, followed by the hydrolysis of the remaining 6-O-beta-D-glycosidic bond (e.g. ginsenoside Re -> ginsenoside Rg1 -> ginsenoside F1).
