| Entry |
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| Name |
20-O-multi-glycoside ginsenosidase;
ginsenosidase type II (erroneous)
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| Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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| Sysname |
protopanaxadiol-type ginsenoside 20-beta-D-glucohydrolase
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| Reaction(IUBMB) |
a protopanaxadiol-type ginsenoside with two glycosyl residues at position 20 + H2O = a protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20 + a monosaccharide
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| Substrate |
protopanaxadiol-type ginsenoside with two glycosyl residues at position 20;
H2O [CPD: C00001]
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| Product |
protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20;
monosaccharide
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| Comment |
The 20-O-multi-glycoside ginsenosidase catalyses the hydrolysis of the 20-O-alpha-(1->6)-glycosidic bond and the 20-O-beta-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20, although it can cleave the remaining glucosyl residue with a lower efficiency. Starting with a ginsenoside that is glycosylated at positions 3 and 20, such as ginsenosides Rb1, Rb2, Rb3 and Rc, the most common product is ginsenoside Rd, with a low amount of ginsenoside Rg3 also formed.
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| History |
EC 3.2.1.195 created 2014
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| Reference |
1 |
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| Authors |
Yu, H., Liu, Q., Zhang, C., Lu, M., Fu, Y., Im, W.-T., Lee, S.-T. and Jin, F. |
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| Title |
A new ginsenosidase from Aspergillus strain hydrolyzing 20-O-multi-glycoside of PPD ginsenoside. |
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| Journal |
Process Biochem 44:772-775 (2009) |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 3.2.1.195 |
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