KEGG   ENZYME: 3.2.1.197Help
Entry
EC 3.2.1.197                Enzyme                                 

Name
beta-1,2-mannosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
beta-1,2-D-mannoside mannohydrolase
Reaction(IUBMB)
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O = beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose [RN:R11544]
Reaction(KEGG)
Substrate
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose [CPD:C21454];
H2O [CPD:C00001]
Product
beta-D-mannopyranosyl-(1->2)-D-mannopyranose [CPD:C21336];
alpha-D-mannopyranose [CPD:C00936]
Comment
The enzyme, characterized from multiple bacterial species, catalyses the hydrolysis of terminal, non-reducing D-mannose residues from beta-1,2-mannotriose and beta-1,2-mannobiose. The mechanism involves anomeric inversion, resulting in the release of alpha-D-mannopyranose. Activity with beta-1,2-mannotriose or higher oligosaccharides is higher than that with beta-1,2-mannobiose.
History
EC 3.2.1.197 created 2016
Orthology
K21065  beta-1,2-mannosidase
Genes
THEF: E1B22_11255
FGI: OP10G_4571
PHM: PSMK_15170
ACA: ACP_0702
ACM: AciX9_3592
TSA: AciPR4_4005
TRS: Terro_0837
TALB: FTW19_09990
ABAS: ACPOL_0357
SUS: Acid_4608
PDI: BDI_3141
PSAC: PSM36_1078
CPI: Cpin_6158
MUC: MuYL_0039
DFE: Dfer_3176
SLI: Slin_3919
GFL: GRFL_1111
ZPR: ZPR_0445
MLT: VC82_1561
 » show all
Taxonomy
Reference
1  [PMID:26286752]
  Authors
Cuskin F, Basle A, Ladeveze S, Day AM, Gilbert HJ, Davies GJ, Potocki-Veronese G, Lowe EC
  Title
The GH130 Family of Mannoside Phosphorylases Contains Glycoside Hydrolases That Target beta-1,2-Mannosidic Linkages in Candida Mannan.
  Journal
J Biol Chem 290:25023-33 (2015)
DOI:10.1074/jbc.M115.681460
  Sequence
[bth:BT_3780]
Reference
2  [PMID:26476324]
  Authors
Nihira T, Chiku K, Suzuki E, Nishimoto M, Fushinobu S, Kitaoka M, Ohtsubo K, Nakai H
  Title
An inverting beta-1,2-mannosidase belonging to glycoside hydrolase family 130 from Dyadobacter fermentans.
  Journal
FEBS Lett 589:3604-10 (2015)
DOI:10.1016/j.febslet.2015.10.008
  Sequence
[dfe:Dfer_3176]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.197
IUBMB Enzyme Nomenclature: 3.2.1.197
ExPASy - ENZYME nomenclature database: 3.2.1.197
BRENDA, the Enzyme Database: 3.2.1.197

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