KEGG ENZYME: 3.2.1.200

ENZYME: 3.2.1.200

Entry

EC 3.2.1.200 Enzyme

Name

exo-chitinase (non-reducing end);
chiB (gene name)

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

(1->4)-2-acetamido-2-deoxy-beta-D-glucan diacetylchitobiohydrolase (non-reducing end)

Reaction(IUBMB)

Hydrolysis of N,N'-diacetylchitobiose from the non-reducing end of chitin and chitodextrins.

Comment

The enzyme hydrolyses the second glycosidic (1->4) linkage from non-reducing ends of chitin and chitodextrin molecules, liberating N,N'-diacetylchitobiose disaccharides. cf. EC 3.2.1.201, exo-chitinase (reducing end).

History

EC 3.2.1.200 created 2017

Reference

1 [PMID:11468293]

Authors

Tanaka T, Fukui T, Imanaka T

Title

Different cleavage specificities of the dual catalytic domains in chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

Journal

J Biol Chem 276:35629-35 (2001)
DOI:10.1074/jbc.M105919200

Sequence

[tko:TK1765]

Reference

2 [PMID:15717865]

Authors

Hult EL, Katouno F, Uchiyama T, Watanabe T, Sugiyama J

Title

Molecular directionality in crystalline beta-chitin: hydrolysis by chitinases A and B from Serratia marcescens 2170.

Journal

Biochem J 388:851-6 (2005)
DOI:10.1042/BJ20050090

Reference

3 [PMID:21390509]

Authors

Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T

Title

A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis.

Journal

Planta 234:123-37 (2011)
DOI:10.1007/s00425-011-1390-3

Sequence

[ath:AT4G19810]

Reference

4 [PMID:23824666]

Authors

Gutierrez-Roman MI, Dunn MF, Tinoco-Valencia R, Holguin-Melendez F, Huerta-Palacios G, Guillen-Navarro K

Title

Potentiation of the synergistic activities of chitinases ChiA, ChiB and ChiC from Serratia marcescens CFFSUR-B2 by chitobiase (Chb) and chitin binding protein (CBP).

Journal

World J Microbiol Biotechnol 30:33-42 (2014)
DOI:10.1007/s11274-013-1421-2

Sequence

[smw:SMWW4_v1c35890]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.200

IUBMB Enzyme Nomenclature:

3.2.1.200

ExPASy - ENZYME nomenclature database:

3.2.1.200

BRENDA, the Enzyme Database:

3.2.1.200

All links

DNA sequence (4)   
   GenBank (2)   
   EMBL (2)   
All databases (4)   

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