KEGG   ENZYME: 3.2.1.200Help
Entry
EC 3.2.1.200                Enzyme                                 

Name
exo-chitinase (non-reducing end);
chiB (gene name)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
(1->4)-2-acetamido-2-deoxy-beta-D-glucan diacetylchitobiohydrolase (non-reducing end)
Reaction(IUBMB)
Hydrolysis of N,N'-diacetylchitobiose from the non-reducing end of chitin and chitodextrins.
Comment
The enzyme hydrolyses the second glycosidic (1->4) linkage from non-reducing ends of chitin and chitodextrin molecules, liberating N,N'-diacetylchitobiose disaccharides. cf. EC 3.2.1.201, exo-chitinase (reducing end).
History
EC 3.2.1.200 created 2017
Reference
1  [PMID:11468293]
  Authors
Tanaka T, Fukui T, Imanaka T
  Title
Different cleavage specificities of the dual catalytic domains in chitinase from  the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.
  Journal
J Biol Chem 276:35629-35 (2001)
DOI:10.1074/jbc.M105919200
  Sequence
[tko:TK1765]
Reference
2  [PMID:15717865]
  Authors
Hult EL, Katouno F, Uchiyama T, Watanabe T, Sugiyama J
  Title
Molecular directionality in crystalline beta-chitin: hydrolysis by chitinases A and B from Serratia marcescens 2170.
  Journal
Biochem J 388:851-6 (2005)
DOI:10.1042/BJ20050090
Reference
3  [PMID:21390509]
  Authors
Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T
  Title
A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis.
  Journal
Planta 234:123-37 (2011)
DOI:10.1007/s00425-011-1390-3
  Sequence
[ath:AT4G19810]
Reference
4  [PMID:23824666]
  Authors
Gutierrez-Roman MI, Dunn MF, Tinoco-Valencia R, Holguin-Melendez F, Huerta-Palacios G, Guillen-Navarro K
  Title
Potentiation of the synergistic activities of chitinases ChiA, ChiB and ChiC from Serratia marcescens CFFSUR-B2 by chitobiase (Chb) and chitin binding protein (CBP).
  Journal
World J Microbiol Biotechnol 30:33-42 (2014)
DOI:10.1007/s11274-013-1421-2
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.200
IUBMB Enzyme Nomenclature: 3.2.1.200
ExPASy - ENZYME nomenclature database: 3.2.1.200
BRENDA, the Enzyme Database: 3.2.1.200

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