KEGG   ENZYME: 3.2.1.201Help
Entry
EC 3.2.1.201                Enzyme                                 

Name
exo-chitinase (reducing end);
chiA (gene name)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
(1->4)-2-acetamido-2-deoxy-beta-D-glucan diacetylchitobiohydrolase (reducing end)
Reaction(IUBMB)
Hydrolysis of N,N'-diacetylchitobiose from the reducing end of chitin and chitodextrins.
Comment
The enzyme hydrolyses the second glycosidic (1->4) linkage from reducing ends of chitin and chitodextrin molecules, liberating N,N'-diacetylchitobiose disaccharides. cf. EC 3.2.1.200, exo-chitinase (non-reducing end).
History
EC 3.2.1.201 created 2017
Reference
1  [PMID:15717865]
  Authors
Hult EL, Katouno F, Uchiyama T, Watanabe T, Sugiyama J
  Title
Molecular directionality in crystalline beta-chitin: hydrolysis by chitinases A and B from Serratia marcescens 2170.
  Journal
Biochem J 388:851-6 (2005)
DOI:10.1042/BJ20050090
Reference
2  [PMID:24168426]
  Authors
Nakagawa YS, Eijsink VG, Totani K, Vaaje-Kolstad G
  Title
Conversion of alpha-chitin substrates with varying particle size and crystallinity reveals substrate preferences of the chitinases and lytic polysaccharide monooxygenase of Serratia marcescens.
  Journal
J Agric Food Chem 61:11061-6 (2013)
DOI:10.1021/jf402743e
Reference
3  [PMID:23824666]
  Authors
Gutierrez-Roman MI, Dunn MF, Tinoco-Valencia R, Holguin-Melendez F, Huerta-Palacios G, Guillen-Navarro K
  Title
Potentiation of the synergistic activities of chitinases ChiA, ChiB and ChiC from Serratia marcescens CFFSUR-B2 by chitobiase (Chb) and chitin binding protein (CBP).
  Journal
World J Microbiol Biotechnol 30:33-42 (2014)
DOI:10.1007/s11274-013-1421-2
Reference
4  [PMID:8757722]
  Authors
Brurberg MB, Nes IF, Eijsink VG
  Title
Comparative studies of chitinases A and B from Serratia marcescens.
  Journal
Microbiology 142 ( Pt 7):1581-9 (1996)
DOI:10.1099/13500872-142-7-1581
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.201
IUBMB Enzyme Nomenclature: 3.2.1.201
ExPASy - ENZYME nomenclature database: 3.2.1.201
BRENDA, the Enzyme Database: 3.2.1.201

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