Entry
Name
mannosyl-oligosaccharide alpha-1,3-glucosidase;
ER glucosidase II;
alpha-glucosidase II;
trimming glucosidase II;
ROT2 (gene name);
GTB1 (gene name);
GANAB (gene name);
PRKCSH (gene name)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
Glc2Man9GlcNAc2-[protein] 3-alpha-glucohydrolase (configuration-inverting)
Reaction(IUBMB)
(1) Glc2Man9GlcNAc2-[protein] + H2O = GlcMan9GlcNAc2-[protein] + beta-D-glucopyranose [RN:
R05980 ];
(2) GlcMan9GlcNAc2-[protein] + H2O = Man9GlcNAc2-[protein] + beta-D-glucopyranose [RN:
R05981 ]
Reaction(KEGG)
Substrate
Glc2Man9GlcNAc2-[protein];
H2O [CPD:
C00001 ];
GlcMan9GlcNAc2-[protein]
Product
GlcMan9GlcNAc2-[protein];
beta-D-glucopyranose [CPD:
C00221 ];
Man9GlcNAc2-[protein]
Comment
This eukaryotic enzyme cleaves off sequentially the two alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature N-glycosylated proteins.
History
EC 3.2.1.207 created 2018
Pathway
Orthology
K05546 mannosyl-oligosaccharide alpha-1,3-glucosidase
Genes
ACYG : 125181874 136788729
ACS : 100556943 100567299(ganab)
PTEX : 113453200 113453201
XLA : 100037025(ganab.L) 108715138
DRE : 100126019(ganaba) 100334730(ganabb)
SRX : 107722752 107730665 107740022 107756491
SANH : 107662060 107676501 107680629 107702959
SGH : 107548499 107550893(ganab) 107556017 107601512
CAUA : 113091080 113094232 113114076
CGIB : 127957918 127970950 128027518
PTET : 122324695(ganaba) 122357430(ganabb) 122358204
LROH : 127164925(ganaba) 127176107(ganabb)
OMC : 131540213(ganaba) 131553183(ganabb)
PPRM : 120460808(ganabb) 120472073(ganaba)
RKG : 130082547(ganaba) 130089770(ganabb)
CIDE : 127494833 127512586
CERY : 137017241(ganabb) 137033658(ganaba)
MASI : 127412623 127425978
TROS : 130564052(ganabb) 130568788(ganaba)
TDW : 130419410(ganaba) 130438257(ganabb)
MANU : 129422200(ganabb) 129441066(ganaba)
IPU : 101154665(ganabb) 108260090
IFU : 128603357 128611512(ganabb)
PHYP : 113529105 113531271
SMEO : 124378759(ganaba) 124386001(ganabb)
TFD : 113634146(ganaba) 113635647(ganabb)
TRN : 134303030 134319478(ganabb)
AMEX : 103032339(ganaba) 103037165(ganabb)
CMAO : 118806827(ganab) 118814403
CHAR : 105906688 105911952(ganabb)
ALIM : 106515999 106536317(ganab)
SASA : 106563465(ganab) 106604381 106612084
STRU : 115168724 115204297 115205583
OTW : 112221157(ganabb) 112231309 112255935
OMY : 110489060(ganab) 110509995(zgc:171967)
OGO : 123993607(ganabb) 124006740(ganaba)
ONE : 115101346 115129232 115130934
OKE : 118381268(ganabb) 118385095
SALP : 111964200 111965939 111967266
SNH : 120021362(ganaba) 120047944(ganabb) 120062434
CCLU : 121533440 121540102 121541857
ARUT : 117398606 117968522
PMRN : 116943169 116953847(GANAB)
DME : Dmel_CG14476(GCS2alpha)
DSI : Dsimw501_GD10555(Dsim_GD10555)
ECOE : 129944726 129945334 129948408
AALB : 109427786 115260715 115267133 115267137
ASUA : 134205520 134208265
CQU : CpipJ_CPIJ009306 CpipJ_CPIJ009308
CPII : 120420408 120420415
CNS : 116341862 116341889 116341890 116347114
BCOO : 119069480 119071884 119084624
LHU : 105669333(GCS2alpha)
VCRB : 124428016 124428017
AROA : 105688132 105689357
TCA : 655389 655466 657174 657250
AGRG : 126743401 126750101 126750246
AGB : 108904462 108904463 108904474 108904478 108904479 108904480 108904484 108907955
DVV : 114327813 114329117 114337325 114337781
PPYR : 116164313 116180232
SLIU : 111358145 111364616
HHAL : 106679031(GCS2alpha)
MRJ : 136846299(GCS2alpha)
CEL : CELE_F40F9.6(aagr-3) CELE_F52D1.1(aagr-4)
CRQ : GCK72_016969 GCK72_022153
BMY : BM_BM4256(Bma-aagr-4)
HRO : HELRODRAFT_139434 HELRODRAFT_74366
MAEA : 128208100 128218781
CSAT : 104727012 104741841 104762526
BNA : 106347941 106348121 106366263
HSYR : 120140897 120181437
PCIN : 129295562 129309535
QSA : O6P43_023407 O6P43_030243
CMAX : 111469732 111471688
CMOS : 111438655 111451543
CPEP : 111777344 111794593
PALZ : 118030879 118043467
CILL : 122303341 122307266
EGT : 105959865 105964866 105976869
SSPL : 121768107 121771418
SHIS : 125206026 125208624
APAN : 127242003 127248738
MING : 122071614 122072856
PSOM : 113275165 113304844
TAES : 123085163 123092957 123098239
PVIR : 120646848 120688281
SMO : SELMODRAFT_142083 SELMODRAFT_169507
ZRO : ZYRO0E01760g ZYRO0E01782g
NCS : NCAS_0H01360(NCAS0H01360)
NDI : NDAI_0F01900(NDAI0F01900)
TPF : TPHA_0B02620(TPHA0B02620)
TBL : TBLA_0A10040(TBLA0A10040)
TDL : TDEL_0G03440(TDEL0G03440)
KAF : KAFR_0G02180(KAFR0G02180)
KNG : KNAG_0M00730(KNAG0M00730)
LBG : 92205210(LODBEIA_P00140) 92206350(LODBEIA_P11540) 92206377(LODBEIA_P11810) 92207302(LODBEIA_P21060) 92208287(LODBEIA_P30910) 92209110(LODBEIA_P39140) 92209920(LODBEIA_P47240) 92209947(LODBEIA_P47510) 92210944(LODBEIA_P57480)
CAL : CAALFM_C500220WA(ROT2)
YLI : 2906941(YALI2_C00247g)
NTE : NEUTE1DRAFT56633(NEUTE1DRAFT_56633)
SMP : 10800990(SMAC4_04391)
TATV : 25785327(TrAtP1_011421)
TASP : 36608181(TrAFT101_008690)
PLJ : 28890350(PLICBS_009685)
CDET : 87940902(CDEST_04399)
BFU : BCIN_02g01070(Bcrot2)
PTRR : 6349150(PtrM4_131080)
ZTR : MYCGRDRAFT_74623(MgAGL4)
CDEP : 91085988(L203_101775)
KMG : 30165205(I203_108496)
KNE : 92177390(IAR55_000130)
CCAC : CcaHIS019_0601500(ROT2)
PPL : POSPLDRAFT_120268 POSPLDRAFT_121535
DSQ : DICSQDRAFT_150074 DICSQDRAFT_183350
FME : FOMMEDRAFT_101639 FOMMEDRAFT_16646
ABP : AGABI1DRAFT72105(AGABI1DRAFT_72105)
ABV : AGABI2DRAFT222103(AGABI2DRAFT_222103)
SCM : SCHCO_02630789(SCHCODRAFT_02630789)
MLR : MELLADRAFT_88331 MELLADRAFT_88334
EHI : EHI_023430(25.t00013)
BLAC : 94349042(CCR75_005289)
EHX : EMIHUDRAFT_415505 EMIHUDRAFT_419333 EMIHUDRAFT_465541
TCR : 507993.260 511279.60
LMAT : 92516577(LSCM1_06653)
LOI : 92362851(LSCM4_07011)
LEIS : 94182292(GH5_07023)
LENR : 94174130(CUR178_06970)
LEIN : 94189428(JIQ42_05807)
PHET : 94292518(JKF63_06492)
TVA : TVAG_2v0200970 TVAG_2v0262360 TVAG_2v0288950 TVAG_2v0460620 TVAG_2v0508420 TVAG_2v0619320 TVAG_2v0662730 TVAG_2v0719050 TVAG_2v0767390 TVAG_2v0954430 TVAG_2v1021840
» show all
Taxonomy
Reference
Authors
Trombetta ES, Simons JF, Helenius A
Title
Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit.
Journal
Sequence
Reference
Authors
Ziak M, Meier M, Etter KS, Roth J
Title
Two isoforms of trimming glucosidase II exist in mammalian tissues and cell lines but not in yeast and insect cells.
Journal
Reference
Authors
Wilkinson BM, Purswani J, Stirling CJ
Title
Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulum.
Journal
Sequence
Reference
Authors
Mora-Montes HM, Bates S, Netea MG, Diaz-Jimenez DF, Lopez-Romero E, Zinker S, Ponce-Noyola P, Kullberg BJ, Brown AJ, Odds FC, Flores-Carreon A, Gow NA
Title
Endoplasmic reticulum alpha-glycosidases of Candida albicans are required for N glycosylation, cell wall integrity, and normal host-fungus interaction.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.2.1.207