| Entry |
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| Name |
exo beta-1,2-glucooligosaccharide sophorohydrolase (non-reducing end)
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| Class |
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
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| Sysname |
exo (1->2)-beta-D-glucooligosaccharide sophorohydrolase (non-reducing end)
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| Reaction(IUBMB) |
[(1->2)-beta-D-glucosyl]n + H2O = sophorose + [(1->2)-beta-D-glucosyl]n-2 [RN: R12634]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
sophorose [CPD: C08250];
[(1->2)-beta-D-glucosyl]n-2 [CPD: C02490]
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| Comment |
The enzyme, characterized from the bacterium Parabacteroides distasonis, specifically hydrolyses (1->2)-beta-D-glucooligosaccharides to sophorose. The best substrates are the tetra- and pentasaccharides. The enzyme is not able to cleave the trisaccharide, and activity with longer linear (1->2)-beta-D-glucans is quite low. This enzyme acts in exo mode and is not able to hydrolyse cyclic (1->2)-beta-D-glucans.
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| History |
EC 3.2.1.214 created 2020
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| Orthology |
| K24289 | exo beta-1,2-glucooligosaccharide sophorohydrolase (non-reducing end) |
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| Genes |
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| Reference |
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| Authors |
Shimizu H, Nakajima M, Miyanaga A, Takahashi Y, Tanaka N, Kobayashi K, Sugimoto N, Nakai H, Taguchi H |
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| Title |
Characterization and Structural Analysis of a Novel exo-Type Enzyme Acting on beta-1,2-Glucooligosaccharides from Parabacteroides distasonis. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 3.2.1.214 |
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