KEGG   ENZYME: 3.2.1.216
Entry
EC 3.2.1.216                Enzyme                                 
Name
kojibiose hydrolase;
kojibiase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
kojibiose glucohydrolase (configuration-inverting)
Reaction(IUBMB)
kojibiose + H2O = beta-D-glucopyranose + D-glucopyranose [RN:R12983]
Reaction(KEGG)
R12983
Substrate
kojibiose [CPD:C15548];
H2O [CPD:C00001]
Product
beta-D-glucopyranose [CPD:C00221];
D-glucopyranose [CPD:C00031]
Comment
The enzyme, characterized from the bacteria Flavobacterium johnsoniae and Mucilaginibacter mallensis, uses anomer-inverting mechanism to release beta-glucose from the non-reducing ends of kojibiose and alpha-1,2-oligoglucans with a higher degree of polymerization.
History
EC 3.2.1.216 created 2022
Orthology
K25766  kojibiose hydrolase
Genes
BXY: BXY_20640
BCEL: BcellWH2_05323(kojP)
BHUM: JXR92_011630
BFC: BacF7301_03410
BFIN: BFINE_14210
BSA: Bacsa_3080
AFD: Alfi_1080
ASH: AL1_13820
ASEN: NQ519_05215
MBAS: ALGA_4412
CFIL: MYF79_21340
NKO: Niako_1955
NIA: A8C56_09625
FLA: SY85_00330
FGG: FSB75_06125
PGIN: FRZ67_10920
FLS: GLV81_01100
LACS: H4075_20830
FLV: KJS94_17255
THEO: IMW88_05175
HHY: Halhy_4691
PEK: FFJ24_008460
PGJ: QG516_03120
SCN: Solca_0103
SLAS: L2B55_01085
MUP: A0256_11590
MUC: MuYL_1472
MGOT: MgSA37_02000(kojP_1)
MUH: HYN43_010385
MGIN: FRZ54_22640
MGK: FSB76_14245
MRUB: DEO27_010505
MGOS: DIU38_010985
MGIK: GO620_008485
MSAB: SNE25_05015
SMON: AWR27_19280
SRHO: HH216_03310
SENF: GJR95_26845
EOL: Emtol_2190
FAE: FAES_0792
FIB: A6C57_09120
HSW: Hsw_1714
HYM: N008_06360
HYG: AUC43_03900
HYP: A0257_12675
HYZ: AXW84_06700
HNV: DDQ68_00620
HYJ: FHG12_01205
HTB: MTX78_01070
HYK: O9Z63_19165
HCW: O3303_17065
HSB: MWH26_03910
HAEI: MUN82_20585
HVL: MUN86_23395
FLM: MY04_4679
FLL: EI427_10900
FYA: KMW28_25650
FKA: KM029_18550
CHRW: KA713_06960
FUA: LVD17_21095
FLW: LVD16_16925
FJO: Fjoh_4428
FPLU: NLG42_16180 NLG42_20465
COC: Coch_1393
CAPN: CBG49_04995
CAPO: HW278_09085
CBAL: M667_18445
POM: MED152_05100
FOP: FNB79_11550
FEK: C1H87_15750
AQA: D1815_14275
MESQ: C7H62_0166
FLG: LV716_00920
FLB: LV704_01635
SPON: HME9304_02629
ALUT: O5O44_07335
EAO: BD94_2545
EMN: M876_09770
EEN: BBD30_14080
ELB: VO54_00171(kojP)
EMG: BBD33_10290
EGO: BBD34_01820
EGM: AYC65_05285
ELZ: FCS00_18420
ELIZ: JCR23_01830
CIO: CEQ15_19285
CORZ: MTP08_03000
CPRV: CYPRO_2649
 » show all
Reference
1  [PMID:34728215]
  Authors
Nakamura S, Nihira T, Kurata R, Nakai H, Funane K, Park EY, Miyazaki T.
  Title
Structure of a bacterial alpha-1,2-glucosidase defines mechanisms of hydrolysis and substrate specificity in GH65 family hydrolases.
  Journal
J Biol Chem 297:101366 (2021)
DOI:10.1016/j.jbc.2021.101366
  Sequence
[fjo:Fjoh_4428]
Reference
2  [PMID:34684901]
  Authors
De Beul E, Jongbloet A, Franceus J, Desmet T.
  Title
Discovery of a Kojibiose Hydrolase by Analysis of Specificity-Determining Correlated Positions in Glycoside Hydrolase Family 65.
  Journal
Molecules 26:6321 (2021)
DOI:10.3390/molecules26206321
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.216
IUBMB Enzyme Nomenclature: 3.2.1.216
ExPASy - ENZYME nomenclature database: 3.2.1.216
BRENDA, the Enzyme Database: 3.2.1.216

  All links  
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (1361)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (89)   
   KEGG MGENES (1271)   
All databases (1367)   

Download RDF
DBGET integrated database retrieval system