KEGG ENZYME: 3.2.1.227

ENZYME: 3.2.1.227

Entry

EC 3.2.1.227                Enzyme

Name

N-acetyllactosaminidase

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

oligosaccharide exo beta-(1,3)-N-acetyllactosamine hydrolase (non-reducing end)

Reaction(IUBMB)

lacto-N-neotetraose + H2O = N-acetyllactosamine + lactose [RN:R13436]

Reaction(KEGG)

R13436(G)

Substrate

lacto-N-neotetraose;
H2O [CPD:C00001]

Product

N-acetyllactosamine [CPD:C00611];
lactose [CPD:C00243]

Comment

The enzyme, characterized from several bacterial species, hydrolyses the terminal N-acetyllactosamine residue (beta-D-Gal-(1->4)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure beta-D-Gal (1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R). The enzyme also catalyses the activity of EC 3.2.1.140, lacto-N-biosidase, and is not active on oligosaccharides with a terminal GlcNAc residue.

History

EC 3.2.1.227 created 2024

Orthology

K27981

N-acetyllactosaminidase

Genes

FPES:

NXS98_16245

LLH:

I41_15320

AGV:	OJF2_23330

LPRO:

PQO03_08500

TDE:

TDE_1723

TPK:	JO40_10595

TPAV:

HRQ91_09375

PFER:

IRI77_04675

PBT:	ING2E5B_2210

PMUC:

ING2E5A_0743

PDI:

BDI_1426

PARC:

CI960_11345

PGOL:

K6V26_08460

PFAI:

NXY11_07915

ASEN:

NQ519_07265

MCOS:

GM418_03060

CPI:

Cpin_3944

CHIH:

GWR21_03850

COY:	HF329_04240 HF329_25880

CFIL:

MYF79_18650

CHF:	KTO58_07615

CSAC:

SIO70_21135

CPOL:

WJU16_16250

CHIM:

CK934_21415

NKO:	Niako_3494

NSO:	NIASO_13285

PGIN:

FRZ67_11265

PGO:	FSB84_04615

PEP:	AQ505_11665

PGJ:	QG516_25130

MUC:

MuYL_2005

MGOT:

MgSA37_00596

MGIN:

FRZ54_23535

CAMU:

CA2015_0349

CXI:	ABWH99_09390

EVI:	Echvi_3931

EST:	DN752_03185

ECHI:

FKX85_18410

EMAI:

KZP23_14150

SPIK:

EXU85_07215

SPIB:

G8759_18150

STAE:

HNV11_16480

SOQ:	LQ777_13590

SFOL:

H3H32_21170

SENF:

GJR95_01005

RHOZ:

GXP67_20835

MAQA:

LAG90_09780

RUN:	DR864_25100

ADD:	HUW48_02465

CLIT:

OQ292_30105

IRO:	RT717_22695

CHK:	D4L85_13970

REK:	N6H18_18345

CNQ:	PZB72_27615

MART:

BTR34_08630

AQR:	HN014_05325

SINH:

LS482_12870

MLT:

VC82_2681

ZSP:	MQE36_04005

VIL:	CFK37_08605

VNE:	CFK40_03730

VSS:	PD280_10170

MEKU:

HUW50_08250

MRL:	WCV66_04470

POW:	IJ21_26600

PBK:	Back11_19830

PSPN:

L1F29_22995

JDA:	BW727_101632

PALM:

RBG61_07260

CCOT:

CCAX7_10720

FSH:	Q2T83_05035

» show all

Reference

1 [PMID:39239753]

Authors

Vuillemin M, Muschiol J, Zhang Y, Holck J, Barrett K, Preben Morth J, Meyer AS, Zeuner B.

Title

Discovery of Lacto-N-Biosidases and a Novel N-Acetyllactosaminidase Activity in the CAZy Family GH20: Functional Diversity and Structural Insights.

Journal

Chembiochem e202400710 (2024)
DOI:10.1002/cbic.202400710

Sequence

[nko:Niako_3494]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.227

IUBMB Enzyme Nomenclature:

3.2.1.227

ExPASy - ENZYME nomenclature database:

3.2.1.227

BRENDA, the Enzyme Database:

3.2.1.227

All links

No link information was found.

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