KEGG ENZYME: 3.2.1.229

ENZYME: 3.2.1.229

Entry

EC 3.2.1.229                Enzyme

Name

chitinosanase;
chitosan hydrolase (ambiguous)

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

partially acetylated chitosan endo glucsamino-N-acetylglucosaminohydrolase (configuration-inverting)

Reaction(IUBMB)

Xm-(1->4)-beta-D-GlcpN-(1->4)-beta-D-GlcpNAc-(1->4)-Xn + H2O = Xm-(1->4)-beta-D-GlcpN-(1->4)-alpha-D-GlcpNAc + Xn

Substrate

Xm-(1->4)-beta-D-GlcpN-(1->4)-beta-D-GlcpNAc-(1->4)-Xn;
H2O [CPD:C00001]

Product

Xm-(1->4)-beta-D-GlcpN-(1->4)-alpha-D-GlcpNAc;
Xn

Comment

The enzyme is an endo hydrolase that cleaves after the GlcN-GlcNAc diad in partially acetylated chitosan with subsite -2 specific for GlcN and subsite -1 specific for GlcNAc. The enzyme does not cleave fully deacetylated chitosans which are cleaved by chitosanases (EC 3.2.1.132) and glucosaminidases (EC 3.2.1.165) or fully acetylated chitins which are cleaved by chitinases (EC 3.2.1.14, EC 3.2.1.200, EC 3.2.1.201, EC 3.2.1.202) and N-acetylglucosaminidases (EC 3.2.1.52). The protein was found in the medium in which the plant pathogenic fungus Alternaria alternata was grown.

History

EC 3.2.1.229 created 2025

Reference

1 [PMID:28821036]

Authors

Kohlhoff M, Niehues A, Wattjes J, Beneteau J, Cord-Landwehr S, El Gueddari NE, Bernard F, Rivera-Rodriguez GR, Moerschbacher BM.

Title

Chitinosanase: A fungal chitosan hydrolyzing enzyme with a new and unusually specific cleavage pattern.

Journal

Carbohydr Polym 174:1121-1128 (2017)
DOI:10.1016/j.carbpol.2017.07.001

Other DBs

ExplorEnz - The Enzyme Database: