KEGG   ENZYME: 3.2.1.67
Entry
EC 3.2.1.67                 Enzyme                                 

Name
galacturonan 1,4-alpha-galacturonidase;
exo-polygalacturonase;
poly(galacturonate) hydrolase (ambiguous);
exo-D-galacturonase;
exo-D-galacturonanase;
exopoly-D-galacturonase;
poly(1,4-alpha-D-galacturonide) galacturonohydrolase (ambiguous);
pgaA (gene name);
pgaB (gene name);
pgaC (gene name);
pgaD (gene name);
pgaE (gene name);
pgaI (gene name);
pgaII (gene name);
poly[(1->4)-alpha-D-galacturonide] galacturonohydrolase;
galacturan 1,4-alpha-galacturonidase (incorrect)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
poly[(1->4)-alpha-D-galacturonide] non-reducing-end galacturonohydrolase
Reaction(IUBMB)
[(1->4)-alpha-D-galacturonide]n + H2O = [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate [RN:R04320 R06201]
Reaction(KEGG)
R04320 R06201(G);
(other) R01982 R07413
Substrate
[(1->4)-alpha-D-galacturonide]n;
H2O [CPD:C00001]
Product
[(1->4)-alpha-D-galacturonide]n-1;
D-galacturonate [CPD:C00333]
Comment
The enzyme hydrolyses the first glycosidic bond from the non-reducing end of the substrate. It is specific for saturated oligomers of D-homogalacturonan, and is unable to degrade unsaturated substrates or methyl-esterified substrates.
History
EC 3.2.1.67 created 1972, modified 2019
Pathway
ec00040  Pentose and glucuronate interconversions
ec01100  Metabolic pathways
Orthology
K01213  galacturan 1,4-alpha-galacturonidase
K22933  galacturan 1,4-alpha-galacturonidase
Genes
ATH: AT1G02790(PGA4) AT3G07820 AT3G07830 AT3G07850 AT3G14040 AT4G18180 AT5G48140
ALY: 9298692 9299951 9306136 9310643 9317333 9317777 9317780 9320735 9320736 9321071 9328186
CRB: 17874179 17874612 17879325 17884988 17887250 17888137 17890685 17892314 17892331 17894364 17900061
CSAT: 104699345 104700137 104703427 104703791 104710461 104714362 104718508 104722810 104723264 104725295 104731719 104738760 104739532 104739617 104740407 104744977 104744978 104744980 104749557 104752514 104756045 104759054 104759259 104760794 104764510 104764511 104764512 104767373 104779872 104784314 104786261
EUS: EUTSA_v10004137mg EUTSA_v10004361mg EUTSA_v10005587mg EUTSA_v10007721mg EUTSA_v10009339mg EUTSA_v10009641mg EUTSA_v10017763mg EUTSA_v10018548mg EUTSA_v10020501mg EUTSA_v10020872mg EUTSA_v10027030mg EUTSA_v10027416mg
BRP: 103828990 103830423 103831485 103835322 103835323 103836643 103843737 103844563 103849010 103849020 103849039 103857474 103858095 103858475 103859243 103859245 103860974 103865247 103865787 103870629(MF16) 103870630 103872502 103874929 103875023 103875286 117132119 117132121 117132135
BNA: 106346714 106348877 106350894 106351472 106352276 106356887 106359168 106359170 106365425 106365426 106374944 106378346 106383935 106384230 106384231 106385399 106385755 106385911 106386224 106386476 106386695 106394103 106395150 106399070 106399073 106399659 106399796 106401622 106408360 106409949 106414786 106423001 106427894 106427912 106427919 106427947 106428991 106429005 106429009 106435698 106436568 106436569 106443469 106443576 106444385 106448957 106448958 106450140 106452868 106453771 106454301 111198712 111201742 111203984 111214122 111214123 111214125 111214126
BOE: 106292192 106293349 106294181 106294434 106295735 106298610 106299493 106303304 106303972 106304455 106305196 106310411 106310699 106320822 106320825 106321808 106325330 106325350 106332959 106333208 106333535 106333568 106333742 106335078 106339179 106341035
RSZ: 108809665 108812932 108813262 108814788 108821064 108821970 108837454 108838179 108842432 108844252 108846575 108850131 108850313 108852316 108852324 108853401 108853648 108853761 108854505 108856287 108857285 108857286 108859817 108860477 108860620 108860724
THJ: 104801285 104806965 104808267 104811276 104811277 104811279 104812499 104818906 104819047 104820122 104820125 104825659 104825959 104826658
LJA: Lj0g3v0307509.1(Lj0g3v0307509.1) Lj0g3v0354459.1(Lj0g3v0354459.1) Lj1g3v0589910.1(Lj1g3v0589910.1) Lj1g3v0589920.1(Lj1g3v0589920.1) Lj2g3v1415270.1(Lj2g3v1415270.1) Lj3g3v0075600.1(Lj3g3v0075600.1) Lj3g3v0075600.2(Lj3g3v0075600.2) Lj3g3v0075600.3(Lj3g3v0075600.3) Lj4g3v2290300.1(Lj4g3v2290300.1) Lj6g3v0607050.1(Lj6g3v0607050.1)
DOSA: Os01t0517500-01(Os01g0517500) Os02t0196700-00(Os02g0196700) Os05t0542800-01(Os05g0542800) Os06t0509600-00(Os06g0509600) Os06t0545200-00(Os06g0545200) Os06t0545400-01(Os06g0545400) Os06t0545800-01(Os06g0545800) Os06t0611400-01(Os06g0611400) Os06t0611500-01(Os06g0611500) Os08t0327200-01(Os08g0327200)
ATS: 109733957(LOC109733957) 109744519(LOC109744519) 109745357(LOC109745357) 109745359(LOC109745359) 109753987(LOC109753987) 109760245(LOC109760245) 109762320(LOC109762320) 109762328(LOC109762328) 109765326(LOC109765326) 109765327(LOC109765327) 109777920(LOC109777920) 109786760(LOC109786760)
PPP: 112276091
SLB: AWJ20_620(PGU1)
NCR: NCU06961(gh28-2)
NTE: NEUTE1DRAFT80051(NEUTE1DRAFT_80051)
MGR: MGG_08752
MAW: MAC_01988
MAJ: MAA_04744
CMT: CCM_08599
ANG: ANI_1_1372164(An18g04810) ANI_1_1598034(An03g06740) ANI_1_1910094(An11g04040) ANI_1_1976014(An01g14650) ANI_1_2108104(An12g07500)
PTE: PTT_08570
CNE: CNC02590
CNB: CNBC4630
ABP: AGABI1DRAFT114753(AGABI1DRAFT_114753) AGABI1DRAFT121554(AGABI1DRAFT_121554) AGABI1DRAFT127199(AGABI1DRAFT_127199)
ABV: AGABI2DRAFT118209(AGABI2DRAFT_118209) AGABI2DRAFT194940(AGABI2DRAFT_194940) AGABI2DRAFT194942(AGABI2DRAFT_194942)
DFA: DFA_12328
BSU: BSU07130(lplD)
BSR: I33_0804
BSL: A7A1_1757
BSH: BSU6051_07130(lplD)
BSUT: BSUB_00786(lplD)
BSUL: BSUA_00786(lplD)
BSUS: Q433_04010
BSS: BSUW23_03635(lplD)
BST: GYO_0973
BSO: BSNT_07099(lplD)
BSQ: B657_07130(lplD)
BSX: C663_0741(lplD)
BJS: MY9_0805
BACY: QF06_02470
BACL: BS34A_08120(lplD)
BALM: BsLM_0752
CSS: Cst_c08480(lplD)
CSD: Clst_0811
TIT: Thit_1733
CSC: Csac_2748
ATE: Athe_2028
TSH: Tsac_0200
PFT: JBW_04342
DTU: Dtur_0440
HUT: Huta_0159
HTI: HTIA_0721
 » show all
Reference
1  [PMID:5661621]
  Authors
Hasegawa S, Nagel CW.
  Title
Isolation of an oligogalacturonate hydrolase from a Bacillus specie.
  Journal
Arch Biochem Biophys 124:513-20 (1968)
DOI:10.1016/0003-9861(68)90360-3
Reference
2  [PMID:16262687]
  Authors
Kluskens LD, van Alebeek GJ, Walther J, Voragen AG, de Vos WM, van der Oost J
  Title
Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima.
  Journal
FEBS J 272:5464-73 (2005)
DOI:10.1111/j.1742-4658.2005.04935.x
Reference
3  [PMID:16822232]
  Authors
Martens-Uzunova ES, Zandleven JS, Benen JA, Awad H, Kools HJ, Beldman G, Voragen AG, Van den Berg JA, Schaap PJ
  Title
A new group of exo-acting family 28 glycoside hydrolases of Aspergillus niger that are involved in pectin degradation.
  Journal
Biochem J 400:43-52 (2006)
DOI:10.1042/BJ20060703
Reference
4  [PMID:19854184]
  Authors
Pijning T, van Pouderoyen G, Kluskens L, van der Oost J, Dijkstra BW
  Title
The crystal structure of a hyperthermoactive exopolygalacturonase from Thermotoga maritima reveals a unique tetramer.
  Journal
FEBS Lett 583:3665-70 (2009)
DOI:10.1016/j.febslet.2009.10.047
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.67
IUBMB Enzyme Nomenclature: 3.2.1.67
ExPASy - ENZYME nomenclature database: 3.2.1.67
BRENDA, the Enzyme Database: 3.2.1.67
CAS: 9045-35-6

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