Entry
Name
purine nucleosidase;
nucleosidase (misleading);
purine beta-ribosidase;
purine nucleoside hydrolase;
purine ribonucleosidase;
ribonucleoside hydrolase (misleading);
nucleoside hydrolase (misleading);
N-ribosyl purine ribohydrolase;
nucleosidase g;
N-D-ribosylpurine ribohydrolase;
inosine-adenosine-guanosine preferring nucleoside hydrolase;
purine-specific nucleoside N-ribohydrolase;
IAG-nucleoside hydrolase;
IAG-NH
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
purine-nucleoside ribohydrolase
Reaction(IUBMB)
a purine nucleoside + H2O = D-ribose + a purine base [RN:
R02341 ]
Reaction(KEGG)
Substrate
Product
Comment
The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [6].
History
EC 3.2.2.1 created 1961, modified 2006, modified 2011
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
Genes
TCR : 508153.640 510315.10
LMAT : 92514583(LSCM1_04568)
LOI : 92359654(LSCM4_03724)
GAI : IMCC3135_31495(rihB_2)
BPY : Bphyt_4161 Bphyt_4926
PMEG : FNZ07_10215 FNZ07_17155
PBRY : NDK50_22835 NDK50_30045
BAN : BA_2888 BA_3606 BA_5338
BAR : GBAA_2888 GBAA_3606 GBAA_5338
BAT : BAS2693 BAS3345 BAS4961
BAH : BAMEG_1024 BAMEG_1711 BAMEG_5393
BAI : BAA_2947 BAA_3635 BAA_5370
BAX : H9401_2756 H9401_3429 H9401_5093
BANT : A16_29180 A16_36130 A16_53530
BANR : A16R_29630 A16R_36590 A16R_54160
BANS : BAPAT_2774 BAPAT_3454 BAPAT_5120
BANH : HYU01_14310 HYU01_17645 HYU01_26095
BANV : DJ46_1658 DJ46_2326 DJ46_4006
BCE : BC2889 BC3552 BC5134
BCA : BCE_2927 BCE_3566 BCE_5237(iunH) BCE_A0076
BCZ : BCE33L2619(iunH) BCE33L3260 BCE33L4823
BCR : BCAH187_A2939 BCAH187_A3569 BCAH187_A5283 BCAH187_C0090
BCB : BCB4264_A2614 BCB4264_A2904 BCB4264_A3659 BCB4264_A5248
BCU : BCAH820_2891 BCAH820_3559 BCAH820_5219 BCAH820_B0062
BCG : BCG9842_B1658 BCG9842_B2170 BCG9842_B2341 BCG9842_B5704
BCQ : BCQ_2726(iunH) BCQ_3345 BCQ_4939
BCX : BCA_2968 BCA_3645 BCA_5245
BAL : BACI_c28560(iunH) BACI_c34950 BACI_c51160
BNC : BCN_2749 BCN_3354 BCN_5032 BCN_P075
BCF : bcf_14130 bcf_17600 bcf_25600 bcf_28005
BCER : BCK_09730 BCK_17505 BCK_20430
BCY : Bcer98_1846 Bcer98_1982 Bcer98_2226
BTK : BT9727_2642(iunH) BT9727_3310 BT9727_4813
BTL : BALH_2595(iunH) BALH_3191(iunH) BALH_4626(iunH)
BTB : BMB171_C2593 BMB171_C3226 BMB171_C4706
BTT : HD73_3089 HD73_3823 HD73_5466
BTHR : YBT1520_15215 YBT1520_18865 YBT1520_27110
BTHI : BTK_15315 BTK_18805 BTK_27080
BTC : CT43_CH2882 CT43_CH3495 CT43_CH3774 CT43_CH5162
BTF : YBT020_14445 YBT020_17390 YBT020_25350
BTM : MC28_2078 MC28_2700 MC28_4366 MC28_G096
BTG : BTB_c30070(rihA2) BTB_c36270(rihA3) BTB_c39030(rihA4) BTB_c53250(rihA5)
BTI : BTG_01625 BTG_04035 BTG_04905 BTG_23065
BTN : BTF1_11970 BTF1_12770 BTF1_15510 BTF1_24055
BTHT : H175_ch2930 H175_ch3553 H175_ch3834 H175_ch5244
BTHU : YBT1518_15920 YBT1518_19480 YBT1518_28570
BTW : BF38_4050 BF38_4725 BF38_862
BTHY : AQ980_03960 AQ980_12205 AQ980_15705
BWE : BcerKBAB4_1745 BcerKBAB4_2695 BcerKBAB4_3254 BcerKBAB4_4926
BWW : bwei_1388(iunH) bwei_2135(iunH) bwei_3142(iunH) bwei_4664(iunH)
BMYO : BG05_2460 BG05_3131 BG05_4102 BG05_924
BTY : Btoyo_0175 Btoyo_0764 Btoyo_2335 Btoyo_4749
BBY : CY96_13240 CY96_16750 CY96_24665 CY96_29130
BWD : CT694_15405 CT694_18890 CT694_27620 CT694_30660
BTRO : FJR70_04690 FJR70_10650 FJR70_19765
BMOB : MLA2C4_15290 MLA2C4_18435
BNT : GSN03_16790 GSN03_24390
BPAC : LMD38_01330 LMD38_09375 LMD38_12655
BPAN : NLJ82_13605 NLJ82_16765 NLJ82_25175 NLJ82_28460
BALU : QRY64_00745 QRY64_13060 QRY64_16555 QRY64_20370 QRY64_28380
BIF : N288_15890 N288_16315
BHAI : KJK41_03595 KJK41_10600
BARD : QRY57_01055 QRY57_17480
BHK : B4U37_04505 B4U37_08015 B4U37_12185
BCOH : BC6307_09290 BC6307_10885
BPF : BpOF4_09550 BpOF4_19070
BON : A361_11340 A361_18380 A361_18780
CFIR : NAF01_03285 NAF01_16490 NAF01_16900
CSPG : LS684_12565 LS684_22035
CPSS : M5V91_02210 M5V91_02645 M5V91_10195
HLI : HLI_03225 HLI_04125 HLI_05245
HNZ : P9989_02720 P9989_08710 P9989_11320
HSHI : MUO14_17285 MUO14_18190 MUO14_21610
HAMY : MUO15_03385 MUO15_05935 MUO15_08920 MUO15_17425
LEX : Len3610_02640 Len3610_09975
FAR : ABE41_003215 ABE41_005680 ABE41_018645
FEC : QNH15_02715 QNH15_07285
PCHU : QNI29_07825 QNI29_10645
MDG : K8L98_05635 K8L98_07305
BVQ : FHE72_03630 FHE72_13490 FHE72_22770
RAZ : U9J35_03370 U9J35_11715 U9J35_13705 U9J35_22050
ROD : P8596_03375 P8596_13970
ROSS : V2W31_03420 V2W31_11650 V2W31_13620
BOU : I5818_06375 I5818_10850
MCUI : G8O30_06410 G8O30_11825
MJO : FOF60_12610 FOF60_15450
MSUT : LC048_07095 LC048_09675
MMAE : MMARE11_11120(iunH)
MBAI : MB901379_01017(rihA)
MKY : IWGMT90018_11720(iunH)
MPHL : MPHLCCUG_01462(rihA)
MTHN : 4412656_01075(rihA)
MAUU : NCTC10437_01243(rihA)
MFLV : NCTC10271_03983(rihA)
MSAL : DSM43276_03509(rihA)
MTER : 4434518_03202(iunH)
CDIP : ERS451417_02365(rihA_2)
CAUI : CAURIS_04355(rihA1)
NFR : ERS450000_01547(rihA)
NAD : NCTC11293_03830(rihA)
RCR : NCTC10994_02838(iunH)
AAGI : NCTC2676_1_00554(rihA)
CGRN : 4412665_00787(rihA)
PAUS : NCTC13651_02109(rihA_1)
» show all
Taxonomy
Reference
Authors
HEPPEL LA, HILMOE RJ.
Title
[Phosphorolysis and hydrolysis of purine ribosides by enzymes from yeast.]
Journal
J Biol Chem 198:683-94 (1952)
Reference
2
Authors
Kalckar HM.
Title
Biosynthetic aspects of nucleosides and nucleic acids.
Journal
Pubbl Staz Zool (Napoli) 23:87-103 (1951)
Reference
Authors
TAKAGI Y, HORECKER BL.
Title
Purification and properties of a bacterial riboside hydrolase.
Journal
J Biol Chem 225:77-86 (1957)
Reference
Authors
TARR HL.
Title
Fish muscle riboside hydrolases.
Journal
Reference
Authors
Parkin DW.
Title
Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei. Purification, specificity, and kinetic mechanism.
Journal
Sequence
Reference
Authors
Ogawa J, Takeda S, Xie SX, Hatanaka H, Ashikari T, Amachi T, Shimizu S.
Title
Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi.
Journal
Reference
Authors
Versees W, Decanniere K, Van Holsbeke E, Devroede N, Steyaert J.
Title
Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax.
Journal
Sequence
Reference
Authors
Mazumder-Shivakumar D, Bruice TC.
Title
Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.1
ExPASy - ENZYME nomenclature database: 3.2.2.1
BRENDA, the Enzyme Database: 3.2.2.1