KEGG   ENZYME: 3.2.2.24
Entry
EC 3.2.2.24                 Enzyme                                 
Name
ADP-ribosyl-[dinitrogen reductase] hydrolase;
azoferredoxin glycosidase;
azoferredoxin-activating enzymes;
dinitrogenase reductase-activating glycohydrolase;
ADP-ribosyl glycohydrolase;
draG (gene name)
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
Sysname
ADP-D-ribosyl-[dinitrogen reductase] ADP-ribosylhydrolase
Reaction(IUBMB)
[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine + H2O = ADP-D-ribose + [dinitrogen reductase]-L-arginine [RN:R03446]
Reaction(KEGG)
R03446
Substrate
[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine [CPD:C01253];
H2O [CPD:C00001]
Product
ADP-D-ribose [CPD:C00301];
[dinitrogen reductase]-L-arginine [CPD:C03315]
Comment
The enzyme restores the activity of EC 1.18.6.1, nitrogenase, by catalysing the removal of ADP-ribose from an arginine residue of the dinitrogenase reductase component of nitrogenase. This activity occurs only when the nitrogenase product, ammonium, is not available. The combined activity of this enzyme and EC 2.4.2.37, NAD+---dinitrogen-reductase ADP-D-ribosyltransferase, controls the level of activity of nitrogenase.
History
EC 3.2.2.24 created 1992
Orthology
K05521  ADP-ribosyl-[dinitrogen reductase] hydrolase
Genes
MMT: Metme_3769
MDN: JT25_015370
MDH: AYM39_06050
MKO: MKLM6_1232
METL: U737_04955(draG)
MPAD: KEF85_02885(draG)
MELL: IVG45_20705(draG)
MMOT: QZJ86_06385(draG)
MEEP: JWZ98_04565(draG)
MEEC: PL263_09125(draG)
MEUP: QC632_07135(draG)
MBUR: EQU24_17290(draG)
MMAI: sS8_5460
MSZE: MSZNOR_4720(draG)
MMAF: GCM100_05500
MTHA: VSX76_07065(draG)
THIS: HZT40_08840(draG)
TUN: J9260_05640(draG)
TLO: J9253_07445(draG)
TSB: HMY34_12995
TSJ: L3K52_17580(draG)
ALV: Alvin_3044
TVI: Thivi_2134
TMB: Thimo_3254
MPUR: MARPU_00285
TSY: THSYN_04005
TTP: E6P07_09455(draG)
THIP: N838_15545(draG)
CJAP: GWK36_11810(draG)
THIM: KFB96_17575(draG)
TBOG: LT988_12110(draG)
TLR: Thiosp_03922(draG)
TFRI: Thiofri_01467(draG_1)
TWG: Thiowin_02585(draG_2)
HHC: M911_09990
EBS: ECTOBSL9_2255
AAEO: BJI67_12565
HAZ: A9404_01640
SEDS: AAY24_16595
GBI: PG2T_00225
VFF: VITFI_CDS1674
AQL: BXU06_13055
RAC: RA876_02725
RLH: MIZ03_0745
HTN: KI616_08020(draG)
RGE: RGE_42850(draG)
RBN: RBXJA2T_11483
IDC: LRM40_03675(draG)
NPV: OHM77_01920(draG)
MEDZ: MTDW_07920(draG)
SLT: Slit_0832
FAM: OYT1_ch1204
FKU: FGKAn22_10480
SEME: MIZ01_0780
SLAC: SKTS_09650
DSU: Dsui_0953
ARES: IWH25_06520(draG)
AIQ: Azoinq_06070(draG)
NIV: JY500_11895(draG)
RBH: B4966_06715
DAR: Daro_1447
DEY: HYN24_06770(draG)
DDEN: KI615_08680(draG)
QAU: KI612_04950(draG)
FLE: KI610_08855(draG)
AHV: AADV58_06370(draG)
AZO: azo0530(draG1)
AOA: dqs_0540
AZA: AZKH_0794(draG)
AZI: AzCIB_0709
AZD: CDA09_08370(draG)
AZR: CJ010_03520(draG)
AZQ: G3580_18980(draG)
ACOM: CEW83_10495(draG)
ZPA: C3497_06280(draG)
APP: CAP2UW1_4428
ACOG: HWD57_08125(draG)
BOY: LQG66_20680(draG)
RPA: TX73_012440(draG)
RPB: RPB_3043
RPC: RPC_0877
RPD: RPD_2408
RPE: RPE_0836
RPT: Rpal_2658
RPX: Rpdx1_3107
MPOL: RZS28_15730(draG)
BVR: BVIR_1910
BLAG: BLTE_24180
RSQ: Rsph17025_3202
CAZT: LV780_15495(draG)
RBL: B6K69_09395
RHC: RGUI_1804
RCP: RCAP_rcc03017(draG)
RAP: RHOA_4113(draG)
AZL: AZL_007740(draG)
ALI: AZOLI_0685(draG)
ABS: AZOBR_70133(draG)
ABQ: ABAZ39_09985
ABF: AMK58_04675
ATI: AL072_00465
AHU: A6A40_02925(draG)
AZT: TSH58p_07620(draG)
AZM: DM194_02575(draG)
AZZ: DEW08_14750(draG)
AOZ: HUE56_19875(draG)
AARE: D3093_13675(draG)
AZS: E6C72_07865(draG)
AZP: E6C67_31575(draG)
ADJ: ACIU1J_13745(draG)
SKT: IGS68_09235(draG)
SROE: JL101_016835(draG)
RRU: Rru_A1008
RRF: F11_05195
RPM: RSPPHO_02586
MGY: MGMSRv2__0347(draG)
MGRY: MSR1_18680(draG)
MAGX: XM1_3936(draG)
MAGN: WV31_08650
DEX: HWD60_15135(draG)
DVN: HQ394_18645(draG)
MAG: amb1576
VSU: P3M64_06900(draG)
AFR: AFE_1525(draG)
AFE: Lferr_1243
AFI: Acife_1653
AFJ: AFERRID_23450
AFP: K1Y48_11500(draG)
MGM: Mmc1_1210
GSU: GSU2800(draG)
GSK: KN400_2737(draG)
GME: Gmet_0680(draG)
GPI: GPICK_12990
GBZ: JZM60_09050(draG)
GEM: GM21_2136
GEB: GM18_1870
GEF: FO488_16870(draG)
GEV: GSVR_40820(draG)
GEC: GEO60473_22290(draG)
GUR: Gura_1208
GEO: Geob_2589(draG)
GLO: Glov_0420
TAMM: GEAMG1_0044(draG)
GBM: Gbem_2082(draG)
GBN: GEOBRER4_11370(draG)
GER: KP004_09410(draG)
GSUB: KP001_05780(draG)
GNT: KP003_08815(draG)
GPL: M1B72_11360(draG)
GHC: L9S41_01350(draG)
PCA: Pcar_2093(draG)
PACE: A6070_04830
PEF: A7E78_00755
PPD: Ppro_3462
DEU: DBW_0107
DVE: DESUT3_36220(draG)
DEP: AOP6_2673(draG)
DES: DSOUD_2575(draG)
DAT: HRM2_09820(draG1)
DHG: EYB58_19360(draG)
DOV: DSCO28_58830(draG)
DVR: Dvar_77700
AFW: Anae109_3034
AORY: AMOR_48950(draG)
APAU: AMPC_39500(draG_3)
AAQW: K1X11_007360(draG)
TMV: OH491_22880(draG)
OBT: OPIT5_01500
DIN: Selin_0258
DAP: Dacet_1043
GTL: EP073_09660(draG)
CNI: Calni_0682
 » show all
Reference
1  [PMID:2506427]
  Authors
Fitzmaurice WP, Saari LL, Lowery RG, Ludden PW, Roberts GP
  Title
Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum.
  Journal
Mol Gen Genet 218:340-7 (1989)
DOI:10.1007/BF00331287
  Sequence
[rru:Rru_A1008]
Reference
2  [PMID:19477184]
  Authors
Li XD, Huergo LF, Gasperina A, Pedrosa FO, Merrick M, Winkler FK
  Title
Crystal structure of dinitrogenase reductase-activating glycohydrolase (DraG) reveals conservation in the ADP-ribosylhydrolase fold and specific features in the ADP-ribose-binding pocket.
  Journal
J Mol Biol 390:737-46 (2009)
DOI:10.1016/j.jmb.2009.05.031
Reference
3  [PMID:19706507]
  Authors
Berthold CL, Wang H, Nordlund S, Hogbom M
  Title
Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of the dimanganese mono-ADP-ribosylhydrolase DraG.
  Journal
Proc Natl Acad Sci U S A 106:14247-52 (2009)
DOI:10.1073/pnas.0905906106
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.24
IUBMB Enzyme Nomenclature: 3.2.2.24
ExPASy - ENZYME nomenclature database: 3.2.2.24
BRENDA, the Enzyme Database: 3.2.2.24
CAS: 125626-63-3

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Gene (93)   
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Protein sequence (1961)   
   UniProt (998)   
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   RefSeq(pep) (939)   
   PDBSTR (22)   
   PMD (1)   
DNA sequence (2526)   
   RefSeq(nuc) (1426)   
   GenBank (552)   
   EMBL (548)   
3D Structure (7)   
   PDB (7)   
Protein domain (4)   
   InterPro (4)   
All databases (4591)   

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