KEGG ENZYME: 3.2.2.24

ENZYME: 3.2.2.24

Entry

EC 3.2.2.24                 Enzyme

Name

ADP-ribosyl-[dinitrogen reductase] hydrolase;
azoferredoxin glycosidase;
azoferredoxin-activating enzymes;
dinitrogenase reductase-activating glycohydrolase;
ADP-ribosyl glycohydrolase;
draG (gene name)

Class

Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds

Sysname

ADP-D-ribosyl-[dinitrogen reductase] ADP-ribosylhydrolase

Reaction(IUBMB)

[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine + H2O = ADP-D-ribose + [dinitrogen reductase]-L-arginine [RN:R03446]

Reaction(KEGG)

R03446

Substrate

[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine [CPD:C01253];
H2O [CPD:C00001]

Product

ADP-D-ribose [CPD:C00301];
[dinitrogen reductase]-L-arginine [CPD:C03315]

Comment

The enzyme restores the activity of EC 1.18.6.1, nitrogenase, by catalysing the removal of ADP-ribose from an arginine residue of the dinitrogenase reductase component of nitrogenase. This activity occurs only when the nitrogenase product, ammonium, is not available. The combined activity of this enzyme and EC 2.4.2.37, NAD+---dinitrogen-reductase ADP-D-ribosyltransferase, controls the level of activity of nitrogenase.

History

EC 3.2.2.24 created 1992

Orthology

K05521

ADP-ribosyl-[dinitrogen reductase] hydrolase

Genes

MMT:	Metme_3769

MDN:	JT25_015370

MDH:	AYM39_06050

MKO:	MKLM6_1232

METL:

U737_04955(draG)

MPAD:

KEF85_02885(draG)

MELL:

IVG45_20705(draG)

MMOT:

QZJ86_06385(draG)

MEEP:

JWZ98_04565(draG)

MEEC:

PL263_09125(draG)

MEUP:

QC632_07135(draG)

MBUR:

EQU24_17290(draG)

MMAI:

sS8_5460

MSZE:

MSZNOR_4720(draG)

MMAF:

GCM100_05500

MTHA:

VSX76_07065(draG)

THIS:

HZT40_08840(draG)

TUN:	J9260_05640(draG)

TLO:	J9253_07445(draG)

TSB:	HMY34_12995

TSJ:	L3K52_17580(draG)

ALV:	Alvin_3044

TVI:	Thivi_2134

TMB:	Thimo_3254

MPUR:

MARPU_00285

TSY:	THSYN_04005

TTP:	E6P07_09455(draG)

THIP:

N838_15545(draG)

CJAP:

GWK36_11810(draG)

THIM:

KFB96_17575(draG)

TBOG:

LT988_12110(draG)

TLR:	Thiosp_03922(draG)

TFRI:

Thiofri_01467(draG_1)

TWG:	Thiowin_02585(draG_2)

HHC:	M911_09990

EBS:	ECTOBSL9_2255

AAEO:

BJI67_12565

HAZ:	A9404_01640

SEDS:

AAY24_16595

GBI:	PG2T_00225

VFF:	VITFI_CDS1674

AQL:	BXU06_13055

RAC:	RA876_02725

RLH:	MIZ03_0745

HTN:	KI616_08020(draG)

RGE:	RGE_42850(draG)

RBN:	RBXJA2T_11483

IDC:	LRM40_03675(draG)

NPV:	OHM77_01920(draG)

SLT:

Slit_0832

FAM:	OYT1_ch1204

FKU:	FGKAn22_10480

SEME:

MIZ01_0780

SLAC:

SKTS_09650

DSU:

Dsui_0953

ARES:

IWH25_06520(draG)

AIQ:	Azoinq_06070(draG)

NIV:	JY500_11895(draG)

RBH:	B4966_06715

DAR:

Daro_1447

DEY:	HYN24_06770(draG)

DDEN:

KI615_08680(draG)

QAU:	KI612_04950(draG)

FLE:	KI610_08855(draG)

AZO:	azo0530(draG1)

AOA:

dqs_0540

AZA:	AZKH_0794(draG)

AZI:	AzCIB_0709

AZD:	CDA09_08370(draG)

AZR:	CJ010_03520(draG)

AZQ:	G3580_18980(draG)

ACOM:

CEW83_10495(draG)

ZPA:	C3497_06280(draG)

APP:	CAP2UW1_4428

BOY:	LQG66_20680(draG)

RPA:	TX73_012440(draG)

RPB:

RPB_3043

RPC:

RPC_0877

RPD:

RPD_2408

RPE:

RPE_0836

RPT:

Rpal_2658

RPX:	Rpdx1_3107

BVR:

BVIR_1910

BLAG:

BLTE_24180

RSQ:	Rsph17025_3202

CAZT:

LV780_15495(draG)

RCP:	RCAP_rcc03017(draG)

RBL:	B6K69_09395

RHC:

RGUI_1804

RAP:	RHOA_4113(draG)

AZL:	AZL_007740(draG)

ALI:	AZOLI_0685(draG)

ABS:	AZOBR_70133(draG)

ABQ:	ABAZ39_09985

ABF:	AMK58_04675

ATI:	AL072_00465

AHU:	A6A40_02925

AZT:	TSH58p_07620(draG)

AZM:	DM194_02575(draG)

AZZ:	DEW08_14750(draG)

AOZ:	HUE56_19875(draG)

AARE:

D3093_13675(draG)

AZS:	E6C72_07865(draG)

SKT:	IGS68_09235(draG)

SROE:

JL101_016835(draG)

RRU:

Rru_A1008

RRF:

F11_05195

RPM:	RSPPHO_02586

MGY:	MGMSRv2__0347(draG)

MGRY:

MSR1_18680(draG)

MAGX:

XM1_3936(draG)

MAGN:

WV31_08650

DEX:	HWD60_15135(draG)

DVN:	HQ394_18645(draG)

MAG:

amb1576

VSU:	P3M64_06900(draG)

MGM:

Mmc1_1210

AFR:	AFE_1525(draG)

AFE:	Lferr_1243

AFI:	Acife_1653

AFJ:	AFERRID_23450

AFP:	K1Y48_11500(draG)

GSU:	GSU2800(draG)

GSK:	KN400_2737(draG)

GME:	Gmet_0680(draG)

GPI:	GPICK_12990

GBZ:	JZM60_09050(draG)

GEM:

GM21_2136

GEB:

GM18_1870

GEF:	FO488_16870(draG)

GEV:	GSVR_40820(draG)

GEC:	GEO60473_22290(draG)

GUR:

Gura_1208

GEO:	Geob_2589(draG)

GLO:

Glov_0420

TAMM:

GEAMG1_0044(draG)

GBM:	Gbem_2082(draG)

GBN:	GEOBRER4_11370(draG)

GER:	KP004_09410(draG)

GSUB:

KP001_05780(draG)

GNT:	KP003_08815(draG)

GPL:	M1B72_11360(draG)

GHC:	L9S41_01350(draG)

PCA:	Pcar_2093(draG)

PACE:

A6070_04830

PEF:	A7E78_00755

PPD:

Ppro_3462

DEU:

DBW_0107

DVE:	DESUT3_36220(draG)

DEP:	AOP6_2673(draG)

DES:	DSOUD_2575(draG)

DAT:	HRM2_09820(draG1)

DOV:	DSCO28_58830(draG)

AFW:	Anae109_3034

AORY:

AMOR_48950(draG)

APAU:

AMPC_39500(draG_3)

AAQW:

K1X11_007360(draG)

TMV:	OH491_22880(draG)

OBT:	OPIT5_01500

DIN:	Selin_0258

DAP:	Dacet_1043

GTL:	EP073_09660(draG)

CNI:	Calni_0682

» show all

Reference

1 [PMID:2506427]

Authors

Fitzmaurice WP, Saari LL, Lowery RG, Ludden PW, Roberts GP

Title

Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum.

Journal

Mol Gen Genet 218:340-7 (1989)
DOI:10.1007/BF00331287

Sequence

[rru:Rru_A1008]

Reference

2 [PMID:19477184]

Authors

Li XD, Huergo LF, Gasperina A, Pedrosa FO, Merrick M, Winkler FK

Title

Crystal structure of dinitrogenase reductase-activating glycohydrolase (DraG) reveals conservation in the ADP-ribosylhydrolase fold and specific features in the ADP-ribose-binding pocket.

Journal

J Mol Biol 390:737-46 (2009)
DOI:10.1016/j.jmb.2009.05.031

Reference

3 [PMID:19706507]

Authors

Berthold CL, Wang H, Nordlund S, Hogbom M

Title

Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of the dimanganese mono-ADP-ribosylhydrolase DraG.

Journal

Proc Natl Acad Sci U S A 106:14247-52 (2009)
DOI:10.1073/pnas.0905906106

Other DBs

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