KEGG ENZYME: 3.2.2.24

ENZYME: 3.2.2.24

Entry

EC 3.2.2.24 Enzyme

Name

ADP-ribosyl-[dinitrogen reductase] hydrolase;
azoferredoxin glycosidase;
azoferredoxin-activating enzymes;
dinitrogenase reductase-activating glycohydrolase;
ADP-ribosyl glycohydrolase;
draG (gene name)

Class

Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds

Sysname

ADP-D-ribosyl-[dinitrogen reductase] ADP-ribosylhydrolase

Reaction(IUBMB)

[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine = ADP-D-ribose + [dinitrogen reductase]-L-arginine [RN:R03446]

Reaction(KEGG)

R03446

Substrate

[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine [CPD:C01253]

Product

ADP-D-ribose [CPD:C00301];
[dinitrogen reductase]-L-arginine [CPD:C03315]

Comment

The enzyme restores the activity of EC 1.18.6.1, nitrogenase, by catalysing the removal of ADP-ribose from an arginine residue of the dinitrogenase reductase component of nitrogenase. This activity occurs only when the nitrogenase product, ammonium, is not available. The combined activity of this enzyme and EC 2.4.2.37, NAD+-dinitrogen-reductase ADP-D-ribosyltransferase, controls the level of activity of nitrogenase.

History

EC 3.2.2.24 created 1992

Orthology

K05521

ADP-ribosyl-[dinitrogen reductase] hydrolase

Genes

SPE:

BTO:

BTRE:

BTRH:

BTRA:

XCC:	XCC2928(draG)

XCB:

XC_1180

XCA:	xcc-b100_1226

SMZ:

SMD_1618

PPF:

Pput_1073

PPW:	PputW619_2513

PPT:

PPI:

PPX:

PPUH:

PPUN:

PMON:

PMOT:

PFL:

PPRC:

PFLCHA0_c41750(draG)

PFO:	Pfl01_3893

PBA:	PSEBR_a3187

SSE:

Ssed_2268

TTU:	TERTU_0344

MMT:	Metme_3769

ALV:	Alvin_3044

TVI:	Thivi_2134

TMB:	Thimo_3254

HHC:	M911_09990

TAU:

Tola_0577

PNA:

Pnap_4684

RGE:	RGE_42850(draG)

SLT:

Slit_0832

DSU:

Dsui_0953

DAR:

Daro_1447

AZO:	azo0530(draG1)

AZA:	AZKH_0794(draG)

AZD:	CDA09_08370(draG)

APP:	CAP2UW1_4428

GSU:	GSU2800(draG)

GSK:	KN400_2737(draG)

GME:	Gmet_0680(draG)

GUR:

Gura_1208

GLO:

Glov_0420

GBM:	Gbem_2082(draG)

GEO:	Geob_2589(draG)

GEM:

GM21_2136

GEB:

GM18_1870

PCA:	Pcar_2093(draG)

PPD:

Ppro_3462

DAT:	HRM2_09820(draG1)

AFW:	Anae109_3034

RPA:	RPA2406(draG)

RPB:

RPC:

RPD:

RPE:

RPT:

RPX:	Rpdx1_3107

MEX:

Mext_4887

MDI:

METDI1913

RSQ:	Rsph17025_3202

RCP:	RCAP_rcc03017(draG)

NAR:

Saro_0259

RRU:

Rru_A1008

RRF:

F11_05195

RPM:	RSPPHO_02586

MAG:

amb1576

MGY:	MGMSRv2__0347(draG)

AZL:	AZL_007740(draG)

ALI:	AZOLI_0685(draG)

ABS:	AZOBR_70133(draG)

MGM:

Mmc1_1210

AFE:	Lferr_1243

AFR:	AFE_1525(draG)

AFI:	Acife_1653

GJF:	M493_17080

PJD:	Pjdr2_4657

GYM:	GYMC10_3507

PPY:

PPE_03341

PPO:	PPM_3580(draG)

PMS:	KNP414_06983

PMQ:	PM3016_6576

PMW:

B2K_33220

PTA:	HPL003_26365

PSAB:

PSAB_03525

CLJ:	CLJU_c19980

CAH:	CAETHG_0078

CCL:	Clocl_0258

RUM:

CK1_14850

STH:

STH1516

DAE:

Dtox_3565

DKU:	Desku_0578

TJR:	TherJR_1018

DED:	DHBDCA_p535

DEC:	DCF50_p593

DRS:	DEHRE_07060

ADG:

Adeg_1446

TTM:

Tthe_0504

TTO:	Thethe_02851

TSH:

Tsac_2819

FMA:

FMG_0339

MMAR:

MODMU_4120

RRD:	RradSPS_1114

AMR:

AM1_1924

ATM:	ANT_00250(draG)

TOS:	Theos_0897

CCZ:	CCALI_00031

TTR:

Tter_0338

CAA:

Caka_2844

DIN:	Selin_0258

DAP:	Dacet_1043

CNI:	Calni_0682

TID:	Thein_0106

MZH:	Mzhil_1549

MPL:

NPH:

HUT:

HTI:

HBO:	Hbor_13350

HLA:

Hlac_2793

HAH:	Halar_0206

NGE:	Natgr_2655

SALI:

L593_06395

» show all

Reference

1 [PMID:2506427]

Authors

Fitzmaurice WP, Saari LL, Lowery RG, Ludden PW, Roberts GP.

Title

Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum.

Journal

Mol Gen Genet 218:340-7 (1989)
DOI:10.1007/BF00331287

Sequence

[rru:Rru_A1008]

Reference

2 [PMID:19477184]

Authors

Li XD, Huergo LF, Gasperina A, Pedrosa FO, Merrick M, Winkler FK

Title

Crystal structure of dinitrogenase reductase-activating glycohydrolase (DraG) reveals conservation in the ADP-ribosylhydrolase fold and specific features in the ADP-ribose-binding pocket.

Journal

J Mol Biol 390:737-46 (2009)
DOI:10.1016/j.jmb.2009.05.031

Reference

3 [PMID:19706507]

Authors

Berthold CL, Wang H, Nordlund S, Hogbom M

Title

Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of the dimanganese mono-ADP-ribosylhydrolase DraG.

Journal

Proc Natl Acad Sci U S A 106:14247-52 (2009)
DOI:10.1073/pnas.0905906106

Other DBs

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