KEGG   ENZYME: 3.4.11.24
Entry
EC 3.4.11.24                Enzyme                                 
Name
aminopeptidase S;
Mername-AA022 peptidase;
SGAP;
aminopeptidase (Streptomyces griseus);
Streptomyces griseus aminopeptidase;
S. griseus AP;
double-zinc aminopeptidase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aminopeptidases
Reaction(IUBMB)
Release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
Comment
Aminopeptidases are associated with many biological functions, including protein maturation, protein degradation, cell-cycle control and hormone-level regulation [3,4]. This enzyme contains two zinc molecules in its active site and is activated by Ca2+ [4]. In the presence of Ca2+, the best substrates are Leu-Phe, Leu-Ser, Leu-pNA (aminoacyl-p-nitroanilide), Phe-Phe-Phe and Phe-Phe [3]. Peptides with proline in the P1' position are not substrates [3]. Belongs in peptidase family M28.
History
EC 3.4.11.24 created 2008
Orthology
K19702  aminopeptidase S
Genes
PMEXH4W19_05095
LABLA76x_2290
LAQGLA29479_4741
LYJFKV23_08410
LSOLGOY17_10765
LSXH8B22_14355
LAREHIV01_005740
AEIAOY20_13825
ALABFG52_11235
ACUMC9E88_015075
ADSFPL17_04915
ALJG8D99_10180
AWGGFH30_08895
ASHAG8E00_06040
AGYLFPL18_16740
SCOSCO4589(SCD20.07c)
SALBXNR_0958
SMASAVERM_4872(lap)
SGRSGR_5809
SGBWQO_06245
SCBSCAB_37611
SSXSACTE_1130
SVLStrvi_0799
SHYSHJG_6556
SHOSHJGH_6316
SRCM271_22010
SMALSMALA_4385 SMALA_6876
SSOII1A49_26000 I1A49_38160
SFASfla_5137
SBHSBI_06122
SVESVEN_4288
SALSSLNWT_4508
STRPF750_1501
SFISFUL_1215
SALUDC74_7273
SALLSAZ_37685
SLVSLIV_15325(lieA)
SGUSGLAU_02315(lieA)
SVTSVTN_22375
STREGZL_01334
SLDT261_0522
SLCSL103_19600 SL103_20730
SXISXIM_11980
STRMM444_20415
STRCAA958_10130
SAMBSAM23877_4424
SPRISPRI_3253
SCZABE83_29585
SCXAS200_20810
SRWTUE45_05961
STRFASR50_23025
SRNA4G23_03276(lieA)
SPAVSpa2297_19400
SCLFBB341_10770
STSIA4E84_23880
SKYD0C37_06180
SALWCP975_28260
SGMGCM10017557_48620
SCADDN051_05370
SPHWNFX46_03370 NFX46_33130
SLSSLINC_4975
SNRSNOUR_05855(lieA)
STRDNI25_13800 NI25_16020
SNWBBN63_13330
SAUOBV401_25760 BV401_38020
SSIAA7J05_08725
SVUB1H20_05425
SGVB1H19_36750
SLAUSLA_4407
SALFSMD44_05158
SALJSMD11_3785
SLXSLAV_16255(lieA2)
STROSTRMOE7_34925 STRMOE7_36025
SFKKY5_4733c KY5_6276c
SNZDC008_20335
SGEDWG14_00558 DWG14_03523(lieA_2)
SLKSLUN_23515
SGDELQ87_16250
SQZFQU76_20285
SCYAEJ357_27295
SASTCD934_11525
STIRDDW44_17555
SKACP970_09635 CP970_17755
SGZC0216_02600
SVNCP980_14235
SHAWCEB94_24245
SRKFGW37_14515
SFICEIZ62_13370
SGALCP966_11220 CP966_21505
SSPODDQ41_18595
SPADDVK44_12355
SFYGFH48_17805
SAQUEJC51_28720
SCAVCVT27_05075
SBYH7H31_01220 H7H31_13515
SRIMCP984_21950 CP984_37305
SSUBCP968_14400
SPHVF9278_30210
SCINCP977_20280
SFUGCNQ36_22895
SPACB1H29_15430
STSUB7R87_19850
SCHACP983_17910
SGXH4W23_23720
SCOECP976_25690
SBATG4Z16_31110
SSPBCP982_24865
SNFJYK04_05033(lieA_1) JYK04_07384
SPLACP981_35425
STUIGCM10017668_41790
SATAC5746_31225
SHUNDWB77_03282(lieA)
SHARHUT13_06765
SFEUIM697_41075
SLIAHA039_13765
SDWK7C20_34175
SCALI6J39_05595
SAUHSU9_031695
SFBCP974_17765
SMOBJ7W19_14225
SPEUCGZ69_20715
SROIIAG44_16440
SGJIAG43_18440
SHKJ2N69_29465
SANUK7396_02195
SDDD9753_27265
SACTDMT42_01750
SGOBtest1122_26150
SINEKI385_39865
SLFJEQ17_27395
SDECL3078_27775
SAOVG3H79_14895
SDURM4V62_11140 M4V62_18650
SYANNRK68_19840
KABB7C62_05135
ARUASPU41_14755 ASPU41_18675
ARQBWQ92_21955
ACRYAC20117_14545 AC20117_15825
ASUFMNQ99_11185 MNQ99_16300
LMOIVV02_18030
TDFH9L22_00700
MUZH4N58_12095
KFLKfla_5609
KQIF1D05_12270
AGRAAGRA3207_002060 AGRA3207_003417
SROSros_0792
NOABKM31_41640
NCXNocox_23525
NGNLCN96_03670
SENSACE_5205
AMDAMED_1036
AMNRAM_05270
AMMAMES_1032
AMZB737_1033
AOIAORI_1025
AJAAJAP_34460
AMYCCU254_16950
AMYBBKN51_39230
AABA4R43_20210
AMYYYIM_09430(lieA2)
AORISD37_36070
APRTMUY14_31605
AROONQK81_17755
PSEAWY02_27520
PSEEFRP1_21215
PSEQAD006_00400
PECQAD017_08225
PHHAFB00_00440
APRECNX65_24430
AMIAmir_4953
SESPBN6_58410(lieA)
SSYIEKG83_20115 EKG83_38670
KPHYAOZ06_01175 AOZ06_30780 AOZ06_38895
LEDBBK82_23070 BBK82_37270
ALOCRK55046 CRK58695 CRK60820
SACEGIY23_14480
ACTUActkin_01195(lieA_3) Actkin_05450(lieA_8)
STPStrop_1408
SAQSare_1367
MAUMicau_2351
MILML5_2463
MICBMicB006_5416
MTUACSH63_13000
MICHFJK98_16960
MTEMGCE86_05215
MCABHXZ27_14170
MSAGGCM10017556_56230
VMAVAB18032_19160
AMSAMIS_36460
AFSAFR_35720
ACTSACWT_3172
AIHAiant_68920
ACTRAsp14428_63040 Asp14428_74090
PLABC6361_02130
PLATC6W10_19540
PFLAPflav_059630
PSUUPsuf_020360
VERHUT12_08535
CATICS0771_34570 CS0771_35120
DFUDfulv_37460
CCAZCOUCH_08675
SNASnas_5560
BVOPan97_33690(ywaD_1)
LPAVPLANPX_2384
GPNPan110_51830
SDYNMal52_50570
PLONPla110_35370(lieA)
PFERIRI77_10690
NOJEJ995_05055
ELBVO54_01494(lieA)
MEMAMMAB1_3205
 » show all
Reference
1  [PMID:2503378]
  Authors
Spungin A, Blumberg S.
  Title
Streptomyces griseus aminopeptidase is a calcium-activated zinc metalloprotein. Purification and properties of the enzyme.
  Journal
Eur J Biochem 183:471-7 (1989)
DOI:10.1111/j.1432-1033.1989.tb14952.x
  Sequence
[sgr:SGR_5809]
Reference
2  [PMID:8444149]
  Authors
Ben-Meir D, Spungin A, Ashkenazi R, Blumberg S
  Title
Specificity of Streptomyces griseus aminopeptidase and modulation of activity by divalent metal ion binding and substitution.
  Journal
Eur J Biochem 212:107-12 (1993)
DOI:10.1111/j.1432-1033.1993.tb17639.x
  Sequence
[sgr:SGR_5809]
Reference
3  [PMID:16080009]
  Authors
Arima J, Uesugi Y, Iwabuchi M, Hatanaka T.
  Title
Study on peptide hydrolysis by aminopeptidases from Streptomyces griseus, Streptomyces septatus and Aeromonas proteolytica.
  Journal
Appl Microbiol Biotechnol 70:541-7 (2006)
DOI:10.1007/s00253-005-0105-8
Reference
4  [PMID:15280041]
  Authors
Fundoiano-Hershcovitz Y, Rabinovitch L, Langut Y, Reiland V, Shoham G, Shoham Y.
  Title
Identification of the catalytic residues in the double-zinc aminopeptidase from Streptomyces griseus.
  Journal
FEBS Lett 571:192-6 (2004)
DOI:10.1016/j.febslet.2004.07.001
Reference
5  [PMID:10771423]
  Authors
Gilboa R, Greenblatt HM, Perach M, Spungin-Bialik A, Lessel U, Wohlfahrt G, Schomburg D, Blumberg S, Shoham G.
  Title
Interactions of Streptomyces griseus aminopeptidase with a methionine product analogue: a structural study at 1.53 A resolution.
  Journal
Acta Crystallogr D Biol Crystallogr 56:551-8 (2000)
DOI:10.1107/S0907444900002420
Other DBs
ExplorEnz - The Enzyme Database: 3.4.11.24
IUBMB Enzyme Nomenclature: 3.4.11.24
ExPASy - ENZYME nomenclature database: 3.4.11.24
BRENDA, the Enzyme Database: 3.4.11.24

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