Entry
Name
[mycofactocin precursor peptide] peptidase;
mftE (gene name)
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Dipeptidyl-peptidases and tripeptidyl-peptidases
BRITE hierarchy
Sysname
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one hydrolyase
Reaction(IUBMB)
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O = C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [RN:
R12801 ]
Reaction(KEGG)
Substrate
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:
C22365 ];
H2O [CPD:
C00001 ]
Product
C-terminal [mycofactocin precursor peptide]-glycine [CPD:
C22407 ];
3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:
C22330 ]
Comment
Requires Fe2+ ad Zn2+. The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. It catalyses cleavage of the mycofactocin precursor peptide following its modification by MftC to liberate its final two residues, which consist of a cross-linked valine-tyramine dipeptide.
History
EC 3.4.14.14 created 2021
Orthology
K24697 mycofactocin precursor peptide peptidase
Genes
MAVM : MAA44156_03919(crnA)
MSIM : MSIM_16060 MSIM_51540
MBAI : MB901379_00884(crnA)
MKY : IWGMT90018_10180(mftE)
MPHL : MPHLCCUG_01295(crnA)
MTHN : 4412656_00905(crnA)
MAUU : NCTC10437_01081(crnA)
MFLV : NCTC10271_04118(crnA)
MBUG : MU0053_003830(mftE)
MSAL : DSM43276_03626(crnA)
MTER : 4434518_03333(crnA)
NFR : ERS450000_00861(crnA)
NAD : NCTC11293_04502(crnA)
NWL : NWFMUON74_27100 NWFMUON74_31310
RRT : 4535765_03559(crnA_1)
RCR : NCTC10994_03695(crnA_2)
RANT : RHODO2019_14285(mftE)
FSB : GCM10025867_43730(mftE)
JTR : V3C34_00440(mftE) V3C34_04860(mftE)
NARO : CFH99_21855(mftE) CFH99_24460(mftE)
NAQU : ENKNEFLB_00511(mftE)
AWS : ACN3XK_26850(mftE) ACN3XK_27000(mftE)
SACG : FDZ84_23090(mftE) FDZ84_34710(mftE)
AMOG : QRX60_23445(mftE) QRX60_42435(mftE)
ANW : QP939_20580(mftE) QP939_43780(mftE)
ABAR : AMYBAR_005653(mftE)
SBAE : DSM104329_01721(mftE_2)
» show all
Taxonomy
Reference
Authors
Bruender NA, Bandarian V
Title
The Creatininase Homolog MftE from Mycobacterium smegmatis Catalyzes a Peptide Cleavage Reaction in the Biosynthesis of a Novel Ribosomally Synthesized Post-translationally Modified Peptide (RiPP).
Journal
Sequence
Reference
Authors
Ayikpoe R, Salazar J, Majestic B, Latham JA
Title
Mycofactocin Biosynthesis Proceeds through 3-Amino-5-[( p-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone (AHDP); Direct Observation of MftE Specificity toward MftA.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 3.4.14.14