KEGG ENZYME: 3.4.14.14

ENZYME: 3.4.14.14

Entry

EC 3.4.14.14                Enzyme

Name

[mycofactocin precursor peptide] peptidase;
mftE (gene name)

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Dipeptidyl-peptidases and tripeptidyl-peptidases

Sysname

C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one hydrolyase

Reaction(IUBMB)

C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O = C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [RN:R12801]

Reaction(KEGG)

R12801

Substrate

C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:C22365];
H2O [CPD:C00001]

Product

C-terminal [mycofactocin precursor peptide]-glycine [CPD:C22407];
3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:C22330]

Comment

Requires Fe2+ ad Zn2+. The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. It catalyses cleavage of the mycofactocin precursor peptide following its modification by MftC to liberate its final two residues, which consist of a cross-linked valine-tyramine dipeptide.

History

EC 3.4.14.14 created 2021

Orthology

K24697

mycofactocin precursor peptide peptidase

Genes

RBK:	E0H22_05970

SSED:

H9L14_14825

SPAP:

H3Z74_03120

DEX:	HWD60_01655(mftE)

DVN:	HQ394_08750

MTU:

Rv0695

MTV:

RVBD_0695

MTC:

MT0722

MRA:

MRA_0703

MTF:	TBFG_10709

MTB:	TBMG_00708

MTK:	TBSG_00712

MTZ:	TBXG_000701

MTG:	MRGA327_04360

MTI:	MRGA423_04335

MTE:	CCDC5079_0646

MTUR:

CFBS_0731

MTL:	CCDC5180_0637

MTO:	MTCTRI2_0711

MTD:

UDA_0695

MTN:	ERDMAN_0767

MTJ:	J112_03720

MTUB:

MT7199_0713

MTUE:

J114_03710

MTX:	M943_03615

MTUH:

I917_04950

MTUL:

TBHG_00690

MTUT:

HKBT1_0731

MTUU:

HKBT2_0732

MTQ:	HKBS1_0731

MBO:	BQ2027_MB0714

MBB:

BCG_0744

MBT:

JTY_0714

MBM:	BCGMEX_0715

MBK:	K60_007410

MBX:

BCGT_0487

MAF:

MAF_07040

MMIC:

RN08_0775

MCE:	MCAN_06981

MCQ:	BN44_10768

MCV:	BN43_20130

MCX:	BN42_20450

MCZ:	BN45_10796

MPA:

MAP_4155

MAO:

MAP4_4281

MAVI:

RC58_21265

MAVU:

RE97_21315

MAV:

MAV_4476

MIT:

OCO_43670

MIA:

OCU_43430

MID:

MIP_06627

MYO:

OEM_43900

MCHI:

AN480_22760

MIR:

OCQ_44780

MMAL:

CKJ54_20785(mftE)

MLP:

MLM_3632

MMAN:

MMAN_31900(mftE)

MSA:	Mycsm_00995

MUL:

MUL_0775

MMC:

Mmcs_1003

MKM:

Mkms_1020

MJL:

Mjls_1030

MMI:

MMAR_1023

MMAE:

MMARE11_09760

MMM:

W7S_22015

MLI:	MULP_01150

MKN:	MKAN_19600

MYV:	G155_07145

MYE:	AB431_05560

MGO:	AFA91_31790

MHAD:

B586_18785

MDX:	BTO20_28580

MSHG:

MSG_00884(mftE)

MFJ:	MFLOJ_25780(mftE)

MSTO:

MSTO_29420

MSIM:

MSIM_16060 MSIM_51540

MSAK:

MSAS_44590(mftE)

MKU:	I2456_05210(mftE)

MGRO:

FZ046_11360(mftE)

MXE:	MYXE_39970

MNV:	MNVI_25260(mftE)

MPAG:

C0J29_05590(mftE)

MNM:	MNVM_27270(mftE)

MGOR:

H0P51_05085(mftE)

MCOO:

MCOO_41780(mftE)

MBAI:

MB901379_00884(crnA)

MSEO:

MSEO_48270

MPSE:

MPSD_11600

MSHO:

MSHO_28120

MHEK:

JMUB5695_03902

MGAU:

MGALJ_54750(mftE)

MLJ:	MLAC_34930(mftE)

MBRD:

MBRA_06470(mftE)

MSHJ:

MSHI_27500(mftE)

MKR:	MKOR_22760

MDF:	K0O62_05980(mftE)

MMAM:

K3U93_04285(mftE)

MHOL:

K3U96_21195(mftE)

MHER:

K3U94_19110(mftE)

MSEN:

K3U95_06225(mftE)

MPAE:

K0O64_05095(mftE)

MSPG:

F6B93_04325(mftE)

MOT:	LTS72_20550(mftE)

MVM:	MJO54_19075(mftE)

MRF:	MJO55_05345(mftE)

MPAA:

MKK62_20115(mftE)

MCRO:

MI149_05820(mftE)

MSM:	MSMEG_1425

MSG:	MSMEI_1390

MSB:	LJ00_07105

MSN:	LI99_07105

MSH:	LI98_07105

MVA:

Mvan_1298

MGI:

Mflv_5058

MSP:	Mspyr1_44760

MCB:	Mycch_0936

MNE:	D174_06615

MYN:	MyAD_06480

MFT:	XA26_14290

MPHL:

MPHLCCUG_01295(crnA)

MVQ:

MYVA_1103

MLL:	B1R94_05285

MRH:	MycrhN_0706

MTHN:

4412656_00905(crnA)

MHAS:

MHAS_00224(mftE)

MDU:	MDUV_34190(mftE)

MCHT:

MCHIJ_01180(mftE)

MDR:	MDOR_06300

MAUU:

NCTC10437_01081(crnA)

MMAG:

MMAD_11170

MMOR:

MMOR_54160(mftE)

MFX:	MFAL_21340

MAIC:

MAIC_45850

MIJ:	MINS_13350

MALV:

MALV_24310

MTY:	MTOK_31940(mftE)

MPSC:

MPSYJ_53830(mftE)

MARZ:

MARA_27860(mftE)

MGAD:

MGAD_28120

MHEV:

MHEL_11860

MSAR:

MSAR_25570(mftE)

MANY:

MANY_49650

MAUB:

MAUB_22100(mftE)

MPOF:

MPOR_15800(mftE)

MPHU:

MPHO_48460(mftE)

MMUC:

C1S78_022995(mftE)

MMAT:

MMAGJ_42630

MBOK:

MBOE_62310

MFG:	K6L26_25495(mftE)

MSEI:

MSEDJ_23790

MFLV:

NCTC10271_04118(crnA)

MCEE:

MCEL_23170

MAB:

MAB_3832c

MMV:	MYCMA_2119

MABB:

MASS_3844

MABL:

MMASJCM_3848

MCHE:

BB28_19625

MIZ:	BAB75_21900

MSTE:

MSTE_03982

MSAO:

MYCSP_17695

MSAL:

DSM43276_03626(crnA)

MJD:	JDM601_3346

MTER:

4434518_03333(crnA)

MMIN:

MMIN_10480

MHIB:

MHIB_27740(mftE)

ASD:

AS9A_4368

MPAK:

MIU77_15065(mftE)

CLIA:

C3E79_10190

CCYC:

SCMU_09520

NFA:

NFA_32090

NFR:	ERS450000_00861(crnA)

NCY:	NOCYR_3385

NNO:	NONO_c57650

NSL:	BOX37_11270

NOZ:	DMB37_06075(mftE)

NAH:	F5544_26885(mftE)

NAD:	NCTC11293_04502(crnA)

NWL:	NWFMUON74_27100 NWFMUON74_31310

NIE:	KV110_26420(mftE)

NGP:	LTT66_26870(mftE)

RHA:	RHA1_ro06077

RER:

RER_18060

REY:

O5Y_08660

REB:	XU06_08900

RQI:	C1M55_09135(mftE)

ROP:

ROP_61350

ROA:	Pd630_LPD02718

RPY:	Y013_08745

RBY:	CEJ39_19640(mftE)

RHB:

NY08_1945

RAV:	AAT18_18450

RFA:	A3L23_00291(mftE)

RHS:	A3Q41_03123(mftE)

RRZ:	CS378_07970(mftE)

RHU:	A3Q40_00416(mftE)

RHQ:	IM25_00575

RHOD:

AOT96_19500

RRT:	4535765_03559(crnA_1)

RCR:	NCTC10994_03695(crnA_2)

RTM:	G4H71_16315(mftE)

RKO:	JWS14_33135(mftE)

RGO:	KYT97_09530(mftE)

ROZ:	CBI38_20370

REQ:

REQ_36200

GBR:

Gbro_3774

GPO:	GPOL_c36440

GOR:

KTR9_3733

GOQ:	ACH46_15880

GTA:	BCM27_19550

GOC:	CXX93_06980(mftE)

GIT:	C6V83_16175(mftE)

GRU:	GCWB2_19160(crnA)

GOM:	D7316_04930(mftE)

GAV:	C5O27_08985(mftE)

GOD:	GKZ92_18090(mftE)

GAM:	GII34_18030(mftE)

GPD:	GII33_17485(mftE)

GJI:	H1R19_17910(mftE)

GOI:	LK459_15825(mftE)

GHN:	MVF96_18475(mftE)

GAMI:

IHQ52_22055(mftE)

SPIN:

KV203_03680(mftE)

TSM:	ASU32_04100

DTM:	BJL86_2333

DIT:	C3V38_01365(mftE)

DIZ:	CT688_11580(mftE)

DPC:	A6048_12160

DLU:	A6035_11180

DKN:	NHB83_11600(mftE)

TOY:	FO059_05845(mftE)

SHY:

SHJG_2441

SHO:	SHJGH_2206

SRC:	M271_50000

SBRO:

GQF42_07995(mftE)

STUI:

GCM10017668_53900

SPRA:

CP972_11015(mftE)

YIA:	LO772_03290(mftE)

MSED:

E3O41_00730(mftE)

MCAW:

F6J84_03075(mftE)

FRP:	AX769_01380

AARC:

G127AT_05865(mftE)

CPHY:

B5808_07310

HUM:	DVJ78_13575(mftE)

HEA:	HL652_01770(mftE)

CHRE:

IE160_02965(mftE)

SUBA:

LQ955_15005(mftE)

AJG:	KKR91_08555(mftE)

ALX:	LVQ62_05575(mftE)

JLI:	EXU32_11035(mftE)

NDP:	E2C04_03210(mftE)

NARO:

CFH99_21855(mftE) CFH99_24460(mftE)

NAQU:

ENKNEFLB_00511(mftE)

NRO:	K8W59_04800(mftE)

NPC:	KUV85_09750(mftE)

NPS:	KRR39_16390(mftE)

PSIM:

KR76_24785

AEZ:	C3E78_15420(mftE)

AEB:	C6I20_03065(mftE)

AEF:	GEV26_02920(mftE)

SAIU:

J4H86_09350(mftE)

TCU:

Tcur_2360

ACTW:

F7P10_02085(mftE)

FRE:	Franean1_3204

FRI:	FraEuI1c_0994

FAL:

FRAAL5874

FSY:	FsymDg_2777

NML:

Namu_0769

GOB:

Gobs_4930

BSD:	BLASA_4809

BLAP:

MVA48_17075(mftE)

MMAR:

MODMU_5385

SEN:

SACE_2391

SACG:

FDZ84_23090(mftE) FDZ84_34710(mftE)

AMQ:	AMETH_4930

AMYC:

CU254_14355(mftE)

AACD:

LWP59_29675(mftE)

APRT:

MUY14_18350(mftE)

PDX:

Psed_5679

PSEA:

WY02_13530

PSEE:

FRP1_00660

PSEH:

XF36_00085

PSEQ:

AD006_08300

PECQ:

AD017_16130

PHH:	AFB00_13940

PAUT:

Pdca_61380

PBRO:

HOP40_10815(mftE)

ALO:

CRK56116

PMAD:

BAY61_05990

ASE:

ACPL_6043

AMS:	AMIS_27200

ACTS:

ACWT_5911

ATL:	Athai_38640

CATI:

CS0771_28390 CS0771_62050(mftE)

NAV:	JQS30_07140

EKE:	EK0264_07285(mftE)

RXY:

Rxyl_3150

RRD:	RradSPS_2638

RMAR:

GBA65_20420(mftE)

SBAE:

DSM104329_01721(mftE_2)

AYM:	YM304_36370

AQZ:	KSP35_11430(mftE)

IAM:	HC251_02935(mftE)

» show all

Reference

1 [PMID:28077628]

Authors

Bruender NA, Bandarian V

Title

The Creatininase Homolog MftE from Mycobacterium smegmatis Catalyzes a Peptide Cleavage Reaction in the Biosynthesis of a Novel Ribosomally Synthesized Post-translationally Modified Peptide (RiPP).

Journal

J Biol Chem 292:4371-4381 (2017)
DOI:10.1074/jbc.M116.762062

Sequence

[msh:LI98_07105]

Reference

2 [PMID:30183269]

Authors

Ayikpoe R, Salazar J, Majestic B, Latham JA

Title

Mycofactocin Biosynthesis Proceeds through 3-Amino-5-[( p-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone (AHDP); Direct Observation of MftE Specificity toward MftA.

Journal

Biochemistry 57:5379-5383 (2018)
DOI:10.1021/acs.biochem.8b00816

Other DBs

ExplorEnz - The Enzyme Database:

3.4.14.14

IUBMB Enzyme Nomenclature:

3.4.14.14

ExPASy - ENZYME nomenclature database:

3.4.14.14

BRENDA, the Enzyme Database:

3.4.14.14

All links

Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (1)   
   KEGG REACTION (1)   
Gene (308)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (307)   
All databases (313)   

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