KEGG   ENZYME: 3.4.14.14
Entry
EC 3.4.14.14                Enzyme                                 
Name
[mycofactocin precursor peptide] peptidase;
mftE (gene name)
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Dipeptidyl-peptidases and tripeptidyl-peptidases
Sysname
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one hydrolyase
Reaction(IUBMB)
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O = C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [RN:R12801]
Reaction(KEGG)
R12801
Substrate
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:C22365];
H2O [CPD:C00001]
Product
C-terminal [mycofactocin precursor peptide]-glycine [CPD:C22407];
3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:C22330]
Comment
Requires Fe2+ ad Zn2+. The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. It catalyses cleavage of the mycofactocin precursor peptide following its modification by MftC to liberate its final two residues, which consist of a cross-linked valine-tyramine dipeptide.
History
EC 3.4.14.14 created 2021
Orthology
K24697  mycofactocin precursor peptide peptidase
Genes
RBKE0H22_05970
SSEDH9L14_14825
SPAPH3Z74_03120
DEXHWD60_01655(mftE)
DVNHQ394_08750
MTURv0695
MTVRVBD_0695
MTCMT0722
MRAMRA_0703
MTFTBFG_10709
MTBTBMG_00708
MTKTBSG_00712
MTZTBXG_000701
MTGMRGA327_04360
MTIMRGA423_04335
MTECCDC5079_0646
MTURCFBS_0731
MTLCCDC5180_0637
MTOMTCTRI2_0711
MTDUDA_0695
MTNERDMAN_0767
MTJJ112_03720
MTUBMT7199_0713
MTUEJ114_03710
MTXM943_03615
MTUHI917_04950
MTULTBHG_00690
MTUTHKBT1_0731
MTUUHKBT2_0732
MTQHKBS1_0731
MBOBQ2027_MB0714
MBBBCG_0744
MBTJTY_0714
MBMBCGMEX_0715
MBKK60_007410
MBXBCGT_0487
MAFMAF_07040
MMICRN08_0775
MCEMCAN_06981
MCQBN44_10768
MCVBN43_20130
MCXBN42_20450
MCZBN45_10796
MPAMAP_4155
MAOMAP4_4281
MAVIRC58_21265
MAVURE97_21315
MAVMAV_4476
MITOCO_43670
MIAOCU_43430
MIDMIP_06627
MYOOEM_43900
MCHIAN480_22760
MIROCQ_44780
MMALCKJ54_20785(mftE)
MLPMLM_3632
MMANMMAN_31900(mftE)
MSAMycsm_00995
MULMUL_0775
MMCMmcs_1003
MKMMkms_1020
MJLMjls_1030
MMIMMAR_1023
MMAEMMARE11_09760
MMMW7S_22015
MLIMULP_01150
MKNMKAN_19600
MYVG155_07145
MYEAB431_05560
MGOAFA91_31790
MHADB586_18785
MDXBTO20_28580
MSHGMSG_00884(mftE)
MFJMFLOJ_25780(mftE)
MSTOMSTO_29420
MSIMMSIM_16060 MSIM_51540
MSAKMSAS_44590(mftE)
MKUI2456_05210(mftE)
MGROFZ046_11360(mftE)
MXEMYXE_39970
MNVMNVI_25260(mftE)
MPAGC0J29_05590(mftE)
MNMMNVM_27270(mftE)
MGORH0P51_05085(mftE)
MCOOMCOO_41780(mftE)
MBAIMB901379_00884(crnA)
MSEOMSEO_48270
MPSEMPSD_11600
MSHOMSHO_28120
MHEKJMUB5695_03902
MGAUMGALJ_54750(mftE)
MLJMLAC_34930(mftE)
MBRDMBRA_06470(mftE)
MSHJMSHI_27500(mftE)
MKRMKOR_22760
MDFK0O62_05980(mftE)
MMAMK3U93_04285(mftE)
MHOLK3U96_21195(mftE)
MHERK3U94_19110(mftE)
MSENK3U95_06225(mftE)
MPAEK0O64_05095(mftE)
MSPGF6B93_04325(mftE)
MOTLTS72_20550(mftE)
MVMMJO54_19075(mftE)
MRFMJO55_05345(mftE)
MPAAMKK62_20115(mftE)
MCROMI149_05820(mftE)
MSMMSMEG_1425
MSGMSMEI_1390
MSBLJ00_07105
MSNLI99_07105
MSHLI98_07105
MVAMvan_1298
MGIMflv_5058
MSPMspyr1_44760
MCBMycch_0936
MNED174_06615
MYNMyAD_06480
MFTXA26_14290
MPHLMPHLCCUG_01295(crnA)
MVQMYVA_1103
MLLB1R94_05285
MRHMycrhN_0706
MTHN4412656_00905(crnA)
MHASMHAS_00224(mftE)
MDUMDUV_34190(mftE)
MCHTMCHIJ_01180(mftE)
MDRMDOR_06300
MAUUNCTC10437_01081(crnA)
MMAGMMAD_11170
MMORMMOR_54160(mftE)
MFXMFAL_21340
MAICMAIC_45850
MIJMINS_13350
MALVMALV_24310
MTYMTOK_31940(mftE)
MPSCMPSYJ_53830(mftE)
MARZMARA_27860(mftE)
MGADMGAD_28120
MHEVMHEL_11860
MSARMSAR_25570(mftE)
MANYMANY_49650
MAUBMAUB_22100(mftE)
MPOFMPOR_15800(mftE)
MPHUMPHO_48460(mftE)
MMUCC1S78_022995(mftE)
MMATMMAGJ_42630
MBOKMBOE_62310
MFGK6L26_25495(mftE)
MSEIMSEDJ_23790
MFLVNCTC10271_04118(crnA)
MCEEMCEL_23170
MABMAB_3832c
MMVMYCMA_2119
MABBMASS_3844
MABLMMASJCM_3848
MCHEBB28_19625
MIZBAB75_21900
MSTEMSTE_03982
MSAOMYCSP_17695
MSALDSM43276_03626(crnA)
MJDJDM601_3346
MTER4434518_03333(crnA)
MMINMMIN_10480
MHIBMHIB_27740(mftE)
ASDAS9A_4368
MPAKMIU77_15065(mftE)
CLIAC3E79_10190
CCYCSCMU_09520
NFANFA_32090
NFRERS450000_00861(crnA)
NCYNOCYR_3385
NNONONO_c57650
NSLBOX37_11270
NOZDMB37_06075(mftE)
NAHF5544_26885(mftE)
NADNCTC11293_04502(crnA)
NWLNWFMUON74_27100 NWFMUON74_31310
NIEKV110_26420(mftE)
NGPLTT66_26870(mftE)
RHARHA1_ro06077
RERRER_18060
REYO5Y_08660
REBXU06_08900
RQIC1M55_09135(mftE)
ROPROP_61350
ROAPd630_LPD02718
RPYY013_08745
RBYCEJ39_19640(mftE)
RHBNY08_1945
RAVAAT18_18450
RFAA3L23_00291(mftE)
RHSA3Q41_03123(mftE)
RRZCS378_07970(mftE)
RHUA3Q40_00416(mftE)
RHQIM25_00575
RHODAOT96_19500
RRT4535765_03559(crnA_1)
RCRNCTC10994_03695(crnA_2)
RTMG4H71_16315(mftE)
RKOJWS14_33135(mftE)
RGOKYT97_09530(mftE)
ROZCBI38_20370
REQREQ_36200
GBRGbro_3774
GPOGPOL_c36440
GORKTR9_3733
GOQACH46_15880
GTABCM27_19550
GOCCXX93_06980(mftE)
GITC6V83_16175(mftE)
GRUGCWB2_19160(crnA)
GOMD7316_04930(mftE)
GAVC5O27_08985(mftE)
GODGKZ92_18090(mftE)
GAMGII34_18030(mftE)
GPDGII33_17485(mftE)
GJIH1R19_17910(mftE)
GOILK459_15825(mftE)
GHNMVF96_18475(mftE)
GAMIIHQ52_22055(mftE)
SPINKV203_03680(mftE)
TSMASU32_04100
DTMBJL86_2333
DITC3V38_01365(mftE)
DIZCT688_11580(mftE)
DPCA6048_12160
DLUA6035_11180
DKNNHB83_11600(mftE)
TOYFO059_05845(mftE)
SHYSHJG_2441
SHOSHJGH_2206
SRCM271_50000
SBROGQF42_07995(mftE)
STUIGCM10017668_53900
SPRACP972_11015(mftE)
YIALO772_03290(mftE)
MSEDE3O41_00730(mftE)
MCAWF6J84_03075(mftE)
FRPAX769_01380
AARCG127AT_05865(mftE)
CPHYB5808_07310
HUMDVJ78_13575(mftE)
HEAHL652_01770(mftE)
CHREIE160_02965(mftE)
SUBALQ955_15005(mftE)
AJGKKR91_08555(mftE)
ALXLVQ62_05575(mftE)
JLIEXU32_11035(mftE)
NDPE2C04_03210(mftE)
NAROCFH99_21855(mftE) CFH99_24460(mftE)
NAQUENKNEFLB_00511(mftE)
NROK8W59_04800(mftE)
NPCKUV85_09750(mftE)
NPSKRR39_16390(mftE)
PSIMKR76_24785
AEZC3E78_15420(mftE)
AEBC6I20_03065(mftE)
AEFGEV26_02920(mftE)
SAIUJ4H86_09350(mftE)
TCUTcur_2360
ACTWF7P10_02085(mftE)
FREFranean1_3204
FRIFraEuI1c_0994
FALFRAAL5874
FSYFsymDg_2777
NMLNamu_0769
GOBGobs_4930
BSDBLASA_4809
BLAPMVA48_17075(mftE)
MMARMODMU_5385
SENSACE_2391
SACGFDZ84_23090(mftE) FDZ84_34710(mftE)
AMQAMETH_4930
AMYCCU254_14355(mftE)
AACDLWP59_29675(mftE)
APRTMUY14_18350(mftE)
PDXPsed_5679
PSEAWY02_13530
PSEEFRP1_00660
PSEHXF36_00085
PSEQAD006_08300
PECQAD017_16130
PHHAFB00_13940
PAUTPdca_61380
PBROHOP40_10815(mftE)
ALOCRK56116
PMADBAY61_05990
ASEACPL_6043
AMSAMIS_27200
ACTSACWT_5911
ATLAthai_38640
CATICS0771_28390 CS0771_62050(mftE)
NAVJQS30_07140
EKEEK0264_07285(mftE)
RXYRxyl_3150
RRDRradSPS_2638
RMARGBA65_20420(mftE)
SBAEDSM104329_01721(mftE_2)
AYMYM304_36370
AQZKSP35_11430(mftE)
IAMHC251_02935(mftE)
 » show all
Reference
1  [PMID:28077628]
  Authors
Bruender NA, Bandarian V
  Title
The Creatininase Homolog MftE from Mycobacterium smegmatis Catalyzes a Peptide Cleavage Reaction in the Biosynthesis of a Novel Ribosomally Synthesized Post-translationally Modified Peptide (RiPP).
  Journal
J Biol Chem 292:4371-4381 (2017)
DOI:10.1074/jbc.M116.762062
  Sequence
Reference
2  [PMID:30183269]
  Authors
Ayikpoe R, Salazar J, Majestic B, Latham JA
  Title
Mycofactocin Biosynthesis Proceeds through 3-Amino-5-[( p-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone (AHDP); Direct Observation of MftE Specificity toward MftA.
  Journal
Biochemistry 57:5379-5383 (2018)
DOI:10.1021/acs.biochem.8b00816
Other DBs
ExplorEnz - The Enzyme Database: 3.4.14.14
IUBMB Enzyme Nomenclature: 3.4.14.14
ExPASy - ENZYME nomenclature database: 3.4.14.14
BRENDA, the Enzyme Database: 3.4.14.14

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