KEGG   ENZYME: 3.4.17.25
Entry
EC 3.4.17.25                Enzyme                                 
Name
glutathione-S-conjugate glycine hydrolase;
PCS1 (gene name);
PRC1 (gene name);
CPC (gene name);
ATG42 (gene name);
alr0975 (locus name)
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metallocarboxypeptidases
Sysname
glutathione-S-conjugate glycine hydrolase
Reaction(IUBMB)
a glutathione-S-conjugate + H2O = a [gamma-glutamyl-L-cysteine]-S-conjugate + glycine [RN:R12941]
Reaction(KEGG)
R12941
Substrate
glutathione-S-conjugate;
H2O [CPD:C00001]
Product
[gamma-glutamyl-L-cysteine]-S-conjugate [CPD:C22460];
glycine [CPD:C00037]
Comment
The enzyme participates in a glutathione-mediated detoxification pathway found in plants, algae, fungi, and some bacteria. The enzymes from the plant Arabidopsis thaliana and the yeast Saccharomyces cerevisiae also catalyse the activity of EC 2.3.2.15, glutathione gamma-glutamylcysteinyltransferase (phytochelatin synthase).
History
EC 3.4.17.25 created 2021
Orthology
K25587  glutathione-S-conjugate glycine hydrolase
Genes
CIN: 100178336 100186228
SCLV: 120325959 120340772 120340773
CEL: CELE_F54D5.1(pcs-1)
CBR: CBG_02855(Cbr-pcs-1)
BMY: BM_BM4359(Bma-pcs-1)
LOA: LOAG_03454
NAI: NECAME_01843
HRO: HELRODRAFT_167837 HELRODRAFT_72755
LGI: LOTGIDRAFT_126155 LOTGIDRAFT_137593 LOTGIDRAFT_146582
PCAN: 112567184
BGT: 106057552
GAE: 121384806 121384808
HRF: 124138280
HRJ: 124269210
CRG: 105340851
MYI: 110459071
PMAX: 117327503 117328362
OBI: 106869885
OSN: 115222911
LAK: 106151538 106162300
SMM: Smp_072740
SHX: MS3_00002296(PCS3)
OVI: T265_13386
EGL: EGR_05319
NVE: 5502512 5511053 5515808 5516558
EPA: 110234748 110234785 110245189
ATEN: 116296105 116300871 116305086
ADF: 107347323
AMIL: 114953249
PDAM: 113680149
SPIS: 111336955 111336957 111336959
DGT: 114520332 114520520
XEN: 124435507
HMG: 100213020
ATH: AT1G03980(PCS2) AT5G44070(CAD1)
ALY: 9299691 9325551
CRB: 17874871
CSAT: 104725048 104738906 104754519 104760541 104771564 104778551
EUS: EUTSA_v10003193mg EUTSA_v10003194mg EUTSA_v10007644mg
BRP: 103827929 103839206 103839325 103843677 103848856
BNA: 106365411 106387111 106410475 106410482 106411423 106416883 106417837 106417894
BOE: 106306174 106309808 106316809 106316852 106327302
RSZ: 108806450 108855485 108857205 108859194
THJ: 104801141 104815967
CPAP: 110807653 110807687
CIT: 102626992 102627849 102628145 102628437
CIC: CICLE_v10019812mg CICLE_v10019857mg CICLE_v10020222mg CICLE_v10024202mg
PVY: 116123094 116123100 116143112
MINC: 123209706 123215385
TCC: 18613100 18613101
GRA: 105766126
GHI: 107922692 107922779 107937656 107955409
GAB: 108468678 108487459
DZI: 111305317 111305318
EGR: 104416196 104429807 104456285 104456288
GMX: 100778828 100782042 100808418 100813002 547627(PCS1)
GSJ: 114373175 114374931 114398368 114406940 114406941
PVU: PHAVU_001G162600g PHAVU_001G1627001
VRA: 106757134 106757457
VAR: 108323244 108323248
VUN: 114163757 114163774
CCAJ: 109803358 109803463 109803580 109803796
APRC: 113863844 113863845
MTR: 25499283 25499284
TPRA: 123889283 123891319 123915030 123916938 123918006 123918007
CAM: 101494765 101495095
PSAT: 127126013 127126023
LJA: Lj1g3v4515570.1(Lj1g3v4515570.1) Lj1g3v4515590.1(Lj1g3v4515590.1) Lj5g3v1117270.1(Lj5g3v1117270.1) Lj5g3v1117290.1(Lj5g3v1117290.1) Lj6g3v0927380.1(Lj6g3v0927380.1)
ADU: 107477440 107490548 107491737 107491738
AIP: 107634183 107642091 107646261 107646262
AHF: 112695181 112735758 112751025 112758743 112758744 112789329 112799809
LANG: 109333647 109334561 109334562
PCIN: 129293406 129306075
FVE: 101294901 101304357
RCN: 112172820 112174178
PPER: 18768401 18768506
PMUM: 103334440 103334442
PAVI: 110757423 110757529
PDUL: 117637360 117638181
MDM: 103435737 103435918 103445605
MSYL: 126623652 126624438
PXB: 103928761 103928762 103958854
ZJU: 107425745 107425778 107425788 107425809 107425810 107425813
MNT: 21389378 21389379
CSAV: 115695043 115712876 115712877
CSV: 101205022 101205258 101206852
CMO: 103497160 103497195 103498912 103498913 103499404
BHJ: 120067127 120068464
MCHA: 111024196
CMAX: 111488492 111488495
CMOS: 111456253 111456254
CPEP: 111807202 111807203
RCU: 8279693
JCU: 105644508
HBR: 110663356 110663371
MESC: 110630093
POP: 7455714 7464424
PEU: 105115283 105119463
PALZ: 118039661 118039662
JRE: 108996032 108996048
CILL: 122305365 122305366
QSU: 112031602 112031627 112031640
QLO: 115961054 115962285
TWL: 120007930 120011380
VVI: 100266944
VRI: 117918877
SLY: 101251125
SPEN: 107031688
SOT: 102580078 102589889(pcs1) 102600824(pcs6)
SSTN: 125870634
CANN: 107863883
NTA: 107820529(PCS1) 107826728
NSY: 104247600
NTO: 104090966
NAU: 109229239
INI: 109182785
ITR: 116026318
SIND: 105165666 105172177
OEU: 111369747 111403327 111407759 111407761
EGT: 105950186
SSPL: 121749053 121794403
APAN: 127244344 127260027
HAN: 110864979 110936785
ECAD: 122581761 122592595
LSV: 111891546 111893694
CCAV: 112506469 112513093
DCR: 108210641 108220616
CSIN: 114264971 114271994 114272163 114284746 114285270 114302608 114319460
BVG: 104899195 104899197 104899198
SOE: 110787047 110787089 110787091
CQI: 110689750 110689751 110689752 110727974 110727975 110727978
NNU: 104600915(PCS) 104602827 104604383 104610426
MING: 122090863
TSS: 122656950
PSOM: 113280455 113318209 113349886
OSA: 4338799 4339838
DOSA: Os05t0415200-00(Os05g0415200) Os06t0102300-01(Os06g0102300)
OBR: 102706657 102717159
OGL: 127773120 127776707
BDI: 100826819
ATS: 109765261 109779397
TDC: 119304200 119329590 119337742
TAES: 123099057 123108424 123149751 123158478 542834
TUA: 125511537 125520077
LPER: 127317461
SBI: 8079537 8080495
ZMA: 100382428
SITA: 101759348 101760841
SVS: 117858241 117858242
PVIR: 120675585 120705637 120705638
PHAI: 112895072 112895169
PDA: 103706919 103721843 103723014
EGU: 105032919 105055703 105061348
MUS: 103970581 104000433
ZOF: 121971480 121976172 122021446 122052674
DCT: 110107635 110115128
PEQ: 110031890
AOF: 109827790
NCOL: 116252981 116253328
ATR: 18447187
SMO: SELMODRAFT_105477 SELMODRAFT_98967
CRE: CHLRE_07g319500v5
VCN: VOLCADRAFT_33691 VOLCADRAFT_63429
MNG: MNEG_2503
CSL: COCSUDRAFT_19902
CVR: CHLNCDRAFT_25686
APRO: F751_5280
MIS: MICPUN_61050
MPP: MICPUCDRAFT_6746
CME: CYME_CMI111C
TML: GSTUM_00001002001
SPO: SPAC3H1.10
WSE: WALSEDRAFT_7313
WIC: J056_001618
PGR: PGTG_03320 PGTG_03321
MLR: MELLADRAFT_33495
MBR: MONBRDRAFT_12490
DDI: DDB_G0291187
DPP: DICPUDRAFT_53337
DFA: DFA_10091 DFA_10730
ACAN: ACA1_073300 ACA1_255500
TET: TTHERM_00088060
PTM: GSPATT00014601001
SMIN: v1.2.019049.t1(symbB.v1.2.019049.t1) v1.2.028032.t1(symbB.v1.2.028032.t1)
PTI: PHATRDRAFT_9478
FCY: FRACYDRAFT_208526(PCS_1)
TPS: THAPSDRAFT_34418
AAF: AURANDRAFT_3544 AURANDRAFT_53895(PCS1)
PIF: PITG_05730 PITG_05733
PSOJ: PHYSODRAFT_563256
EHX: EMIHUDRAFT_221497 EMIHUDRAFT_43058 EMIHUDRAFT_64521 EMIHUDRAFT_76324
GTT: GUITHDRAFT_144409
NGR: NAEGRDRAFT_32272 NAEGRDRAFT_72098
PJP: LAG73_16485
LSOL: GOY17_00285
LARE: HIV01_001125
PMAI: CF386_06480
SDE: Sde_0516
CBD: CBUD_1504
CBG: CbuG_1436
LLY: J2N86_05825
FRT: F7308_1444
FRF: LO80_01690
FHA: CDV26_04760
FRX: F7310_05910
FAD: CDH04_02960
FMI: F0R74_02890
FSR: KQR59_04500
FRL: FIP56_06165
BMA: BMA1537
BMV: BMASAVP1_A2036
BML: BMA10229_A3276
BMN: BMA10247_1307
BMAL: DM55_185
BMAE: DM78_1851
BMAQ: DM76_165
BMAI: DM57_363
BMAF: DM51_1237
BMAZ: BM44_1817
BMAB: BM45_1379
BPS: BPSL2141
BPM: BURPS1710b_2562
BPL: BURPS1106A_2472
BPD: BURPS668_2416
BPR: GBP346_A2542
BPSE: BDL_3407
BPSM: BBQ_1195
BPSU: BBN_1322
BPSD: BBX_1808
BPZ: BP1026B_I1254
BPQ: BPC006_I2514
BPK: BBK_2823
BPSH: DR55_2444
BPSA: BBU_21
BPSO: X996_2031
BUT: X994_448
BTE: BTH_I2045
BTQ: BTQ_1865
BTJ: BTJ_490
BTZ: BTL_1730
BTD: BTI_1488
BTV: BTHA_1884
BTHE: BTN_3038
BTHM: BTRA_2010
BTHA: DR62_3184
BTHL: BG87_1954
BOK: DM82_1725
BOC: BG90_2995
BUU: WS70_06960
BSAV: WS86_07795
BHG: I6G56_12960
BUL: BW21_1625
BUD: AQ610_07500
BXE: Bxe_B1278
BXB: DR64_6591
BPY: Bphyt_5317
BUZ: AYM40_27085
BFN: OI25_7027
PARB: CJU94_22780
PCAF: DSC91_001347
PMEG: FNZ07_11440
PGIS: I6I06_23215
PDIO: PDMSB3_1821.1
PNU: Pnuc_0547
DOE: DENOEST_3921
FAM: OYT1_ch1124 OYT1_ch2189
SEME: MIZ01_2292
AZI: AzCIB_3132
MOH: IHQ72_34985
KAI: K32_32330
MTW: CQW49_02405
HDI: HDIA_3635
MALG: MALG_03025
YAN: AYJ57_25070
HTQ: FRZ44_04540
DVN: HQ394_03590
PAUU: E8A73_006295
SYNE: Syn6312_3727
CGC: Cyagr_2386
PMT: PMT_1013
PMF: P9303_10791
CMP: Cha6605_0087
LEP: Lepto7376_0734
KOV: K9N68_18925
PSER: ABRG53_1809
CYT: cce_1896
CYJ: Cyan7822_3635
CYN: Cyan7425_3773
TER: Tery_2472
MVAG: D0A34_08760 D0A34_17490
GEI: GEI7407_1721
ONI: Osc7112_2808
MPRO: BJP34_29895
CEP: Cri9333_0746 Cri9333_4423
ANA: alr0975
NPU: Npun_R2659
NOS: Nos7107_2000
NOP: Nos7524_0634
NON: NOS3756_24960
NFL: COO91_00110
NOE: CLI64_02900
AVA: Ava_2958
ANB: ANA_C10574
ANN: EH233_10350
CSG: Cylst_1697
HBQ: QI031_27125
CALT: Cal6303_5233
CALH: IJ00_07355
CALN: NIES2098_37030
NCN: BZZ01_08825
CTHE: Chro_2403
LPRO: PQO03_09365
 » show all
Reference
1  [PMID:12620355]
  Authors
Beck A, Lendzian K, Oven M, Christmann A, Grill E.
  Title
Phytochelatin synthase catalyzes key step in turnover of glutathione conjugates.
  Journal
Phytochemistry 62:423-31 (2003)
DOI:10.1016/s0031-9422(02)00565-4
Reference
2  [PMID:17097087]
  Authors
Grzam A, Tennstedt P, Clemens S, Hell R, Meyer AJ.
  Title
Vacuolar sequestration of glutathione S-conjugates outcompetes a possible degradation of the glutathione moiety by phytochelatin synthase.
  Journal
FEBS Lett 580:6384-90 (2006)
DOI:10.1016/j.febslet.2006.10.050
Reference
3  [PMID:15561184]
  Authors
Harada E, von Roepenack-Lahaye E, Clemens S.
  Title
A cyanobacterial protein with similarity to phytochelatin synthases catalyzes the conversion of glutathione to gamma-glutamylcysteine and lacks phytochelatin synthase activity.
  Journal
Phytochemistry 65:3179-85 (2004)
DOI:10.1016/j.phytochem.2004.09.017
  Sequence
[ana:alr0975]
Reference
4  [PMID:14975765]
  Authors
Tsuji N, Nishikori S, Iwabe O, Shiraki K, Miyasaka H, Takagi M, Hirata K, Miyamoto K.
  Title
Characterization of phytochelatin synthase-like protein encoded by alr0975 from a prokaryote, Nostoc sp. PCC 7120.
  Journal
Biochem Biophys Res Commun 315:751-5 (2004)
DOI:10.1016/j.bbrc.2004.01.122
  Sequence
[ana:alr0975]
Reference
5  [PMID:16339904]
  Authors
Vivares D, Arnoux P, Pignol D.
  Title
A papain-like enzyme at work: native and acyl-enzyme intermediate structures in phytochelatin synthesis.
  Journal
Proc Natl Acad Sci U S A 102:18848-53 (2005)
DOI:10.1073/pnas.0505833102
  Sequence
[ana:alr0975]
Reference
6  [PMID:19897216]
  Authors
Wunschmann J, Krajewski M, Letzel T, Huber EM, Ehrmann A, Grill E, Lendzian KJ.
  Title
Dissection of glutathione conjugate turnover in yeast.
  Journal
Phytochemistry 71:54-61 (2010)
DOI:10.1016/j.phytochem.2009.09.034
Other DBs
ExplorEnz - The Enzyme Database: 3.4.17.25
IUBMB Enzyme Nomenclature: 3.4.17.25
ExPASy - ENZYME nomenclature database: 3.4.17.25
BRENDA, the Enzyme Database: 3.4.17.25

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (1)   
   KEGG REACTION (1)   
Gene (536)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (535)   
All databases (541)   

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