KEGG ENZYME: 3.4.19.1

ENZYME: 3.4.19.1

Entry

EC 3.4.19.1                 Enzyme

Name

acylaminoacyl-peptidase;
acylamino-acid-releasing enzyme;
N-acylpeptide hydrolase;
N-formylmethionine (fMet) aminopeptidase;
alpha-N-acylpeptide hydrolase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Omega peptidases

Reaction(IUBMB)

Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide

Comment

Active at neutral pH. Several variants of this enzyme exist; the human erythrocyte enzyme is relatively specific for removal of N-acetylalanine from peptides. Displays dipeptidyl-peptidase activity on glycyl-peptides, perhaps as a result of mis-recognition of the glycyl residue as an uncharged N-acyl group. Inhibited by diisopropyl fluorophosphate. In peptidase family S9 (prolyl oligopeptidase family).

History

EC 3.4.19.1 created 1978 as EC 3.4.14.3, transferred 1981 to EC 3.4.19.1

Orthology

K01303

acylaminoacyl-peptidase

Genes

HSA:

327(APEH)

PTR:	460376(APEH)

PPS:	100985786(APEH)

GGO:	101141597(APEH)

PON:	100435231(APEH)

NLE:	100582434(APEH)

HMH:	116473461(APEH)

MCC:	705948(APEH)

MCF:	102122091(APEH)

MTHB:

126948654

MNI:	105480452(APEH)

CSAB:

103227638(APEH)

CATY:

105573700(APEH)

PANU:

101017907(APEH)

TGE:	112617349(APEH)

MLEU:

105540339(APEH)

RRO:	104667317(APEH)

RBB:	108526983(APEH)

TFN:	117081498(APEH)

PTEH:

111555409(APEH)

CANG:

105509694(APEH)

CJC:	100396609(APEH)

SBQ:	101046863(APEH)

CIMI:

108286217(APEH)

CSYR:

103265527(APEH)

MMUR:

105874969(APEH)

LCAT:

123623266(APEH)

PCOQ:

105823391(APEH)

OGA:	100956550(APEH)

MMU:	235606(Apeh)

MCAL:

110301693(Apeh)

MPAH:

110327285(Apeh)

RNO:	24206(Apeh)

MCOC:

116072014(Apeh)

ANU:	117693467(Apeh)

MUN:	110565010(Apeh)

CGE:	100761732(Apeh)

MAUA:

101837793(Apeh)

PROB:

127236664(Apeh)

PLEU:

114705222(Apeh)

MORG:

121464287(Apeh)

MFOT:

126499808

AAMP:

119809474(Apeh)

NGI:	103750179(Apeh)

HGL:	101715825(Apeh)

CPOC:

100730493(Apeh)

CCAN:

109692697(Apeh)

DORD:

105994621(Apeh)

DSP:	122095664(Apeh)

NCAR:

124993379

OCU:

100354109

OPI:	101526873(APEH)

TUP:	102483690(APEH)

GVR:	103589554(APEH)

CFA:	476622(APEH)

CLUD:

112665791(APEH)

VVP:	112911391(APEH)

VLG:	121496040(APEH)

AML:	100475477(APEH)

UMR:	103674818(APEH)

UAH:	113256879(APEH)

UAR:	123798667(APEH)

ELK:

111144111

LLV:

125080035

MPUF:

101674069(APEH)

NVS:	122909423(APEH)

ORO:	101385988(APEH)

EJU:	114224998(APEH)

ZCA:	113916812(APEH)

MLX:	118005201(APEH)

NSU:	110577548(APEH)

LWW:	102749980(APEH)

FCA:	101085867(APEH)

PYU:	121011205(APEH)

PBG:	122488715(APEH)

LRUF:

124524147

PTG:	102959601(APEH)

PPAD:

109247810(APEH)

PUC:

125929356

AJU:	106979244(APEH)

HHV:	120229353(APEH)

BTA:	514666(APEH)

BOM:	102269347(APEH)

BIU:	109575998(APEH)

BBUB:

102408064(APEH)

BBIS:

104997203(APEH)

CHX:	102177845(APEH)

OAS:	101103273(APEH)

ODA:	120868232(APEH)

CCAD:

122424810(APEH)

SSC:	396961(APEH)

CFR:	102505664(APEH)

CBAI:

105080254(APEH)

CDK:	105087692(APEH)

VPC:	102536277(APEH)

BACU:

103017766(APEH)

LVE:	103078029(APEH)

OOR:	101276478(APEH)

DLE:	111174387(APEH)

PCAD:

102980272(APEH)

PSIU:

116761695(APEH)

ECB:	100053100(APEH)

EPZ:	103553415(APEH)

EAI:	106830693(APEH)

MYB:	102261178(APEH)

MYD:	102768766(APEH)

MMYO:

118668472(APEH)

MLF:	102438701(APEH)

MNA:	107529454(APEH)

PKL:	118715665(APEH)

HAI:	109376042(APEH)

DRO:	112309903(APEH)

SHON:

118989470(APEH)

AJM:	119036634(APEH)

PDIC:

114501406(APEH)

PHAS:

123830821(APEH)

MMF:	118633158(APEH)

RFQ:	117036653(APEH)

PALE:

102893074(APEH)

PGIG:

120582359(APEH)

PVP:	105302391(APEH)

RAY:	107513356(APEH)

MJV:	108393344(APEH)

TOD:	119260241(APEH)

SARA:

101542521(APEH)

LAV:	100675656(APEH)

TMU:

101358967

ETF:	101664508(APEH)

DNM:	101444501(APEH)

MDO:	100027962(APEH)

GAS:	123249059(APEH)

SHR:	100934071(APEH)

AFZ:

127554724

PCW:	110216886(APEH)

OAA:	100091478(APEH)

GGA:	415926(APEH) 770794(AAREL)

PCOC:

116241103 116241105

MGP:	100548252 104913124

CJO:	107319598 107319599(APEH)

NMEL:

110405049(APEH) 110405050

APLA:

113845085

ACYG:

106048758 106048772

AFUL:

116492926 116493144(APEH)

TGU:	100219182 100231670

LSR:

110469968

SCAN:

103817128

PMOA:

120495691(APEH) 120514120

OTC:	121347344 121347418

PRUF:

121352763 121352923

GFR:	102042072(APEH) 102042238

FAB:	101810254 101815864(APEH)

PHI:	102102558 102102747

PMAJ:

107210481

CCW:

104683426

CBRC:

103614353(APEH) 103614354

ETL:

114058795

ZAB:	102071466 102071645

ACHL:

103805386(APEH)

SVG:	106849242 106849244

FPG:	101919631(APEH) 101919804

FCH:	102059371(APEH) 102059543

CLV:	102097899(APEH)

EGZ:

104132490

NNI:

104023053

PCAO:

104053218(APEH)

ACUN:

113485010

TALA:

116959110 116959532

AFOR:

103900593(APEH)

ACHC:

115353782 115353783(APEH)

HLE:	104828136(APEH) 104828137

AGEN:

126049748 126049749

GCL:	127021485 127021698

LDI:	104348928(APEH) 104351682

MNB:	103780680(APEH)

NNT:	104411501(APEH)

SHAB:

115614320 115614688

DPUB:

104306650 104306674

CPEA:

104388135(APEH)

CUCA:

104059060

AAM:

106492157

AROW:

112960594 112960600

NPD:	112949133 112949150

TGT:	104569948(APEH)

DNE:	112987002 112987003

SCAM:

104147870(APEH) 104147872

ASN:	102378133(APEH)

AMJ:	102565732(APEH)

CPOO:

109318327(APEH)

GGN:	109288868(APEH)

PSS:	102451421(APEH)

CMY:	102935556(APEH)

CPIC:

101935673(APEH)

TST:	117880269(APEH)

CABI:

116831943(APEH)

MRV:	120369275(APEH)

ACS:	100567769(apeh) 103277916

PVT:	110076744(APEH)

SUND:

121920358 121921307(APEH)

PBI:	103058104(APEH) 103063154

PMUR:

107288725

CTIG:

120315024 120315025(APEH)

PGUT:

117679059 117679069

VKO:	123034236 123034255

PMUA:

114591526(APEH)

ZVI:	118080574 118080575(APEH)

GJA:	107109058(APEH) 107109059

STOW:

125429078 125430022

XLA:	108714585(apeh.L)

XTR:	100491896(apeh)

NPR:	108803244(APEH)

RTEM:

120944995(APEH)

BBUF:

120979252(APEH)

BGAR:

122922658(APEH)

DRE:	100333019(apeh) 678599(zgc:136971)

SRX:	107717528 107730641

SANH:

107669673 107698444(apeh)

SGH:	107573312(apeh)

CCAR:

109083451(apeh) 109095384 109110148

CAUA:

113078400 113107799(apeh) 113107954

CGIB:

127963345(zgc:136971) 128019178

PPRM:

120481299 120483563(zgc:136971)

MAMB:

125279882(zgc:136971) 125280535(apeh)

CIDE:

127517488 127517721

MASI:

127415069(zgc:136971) 127424693 127424731

IPU:	108265705(apeh) 108265926(zgc:136971)

PHYP:

113533362(apeh) 113533537

SMEO:

124400033(apeh) 124400352(zgc:136971)

TFD:	113655831(zgc:136971) 113656077

AMEX:

103027063 103034957(apeh)

EEE:	113578880(apeh) 113589455

TRU:	101067673 101069353

TNG:	GSTEN00008970G001 GSTEN00027959G001

LCO:	104928033 104931711

NCC:	104955726(apeh)

CGOB:

115010464 115010876

ELY:	117258664(zgc:136971) 117258822(apeh)

EFO:	125891116(zgc:136971) 125892462(apeh)

PLEP:

121946728(apeh) 121947576(zgc:136971)

SLUC:

116040829(zgc:136971) 116061967(apeh)

ECRA:

117947448(apeh) 117948106(zgc:136971)

ESP:	116692321 116692853

PFLV:

114558796 114559445

GAT:	120829723(zgc:136971) 120829838(apeh)

PPUG:

119199468 119222130(zgc:136971) 119223415

MSAM:

119905439(apeh) 119905713(zgc:136971)

CUD:	121516962(apeh) 121518003(zgc:136971)

ALAT:

119022257(apeh) 119022628(zgc:136971)

MZE:	101469582 101473250

ONL:	100696455 100709967

OAU:	116324687(zgc:136971) 116330781(apeh)

OLA:	101160809 101170588

OML:	112158935(apeh) 112159656(zgc:136971)

XMA:	102220561 102235977

XCO:	114144974 114148491

XHE:	116725001 116727878

PRET:

103467450 103473525(apeh)

PFOR:

103129855 103155924(apeh)

PLAI:

106956352(apeh) 106959473

PMEI:

106930119 106930477(apeh)

GAF:	122824328(apeh) 122831686(zgc:136971)

CVG:	107094859 107098949(apeh)

CTUL:

119784344(apeh) 119789254(zgc:136971)

GMU:	124873754(zgc:136971) 124875143(apeh)

NFU:	107373674 107390611(apeh)

KMR:	108233030(zgc:136971) 108235266(apeh)

ALIM:

106513079(apeh) 106523903 106523914

NWH:	119417593(apeh) 119417693(zgc:136971)

AOCE:

111570009 111574391

MCEP:

124997898(zgc:136971) 125022208(apeh)

CSEM:

103384671 103384986(apeh)

POV:	109630474 109635252

SSEN:

122776629(zgc:136971) 122777359(apeh)

HHIP:

117764187(zgc:136971) 117764815(apeh)

HSP:	118111009(apeh) 118111426(zgc:136971)

LCF:	108877888 108891729(zgc:136971)

SDU:	111218987 111230715

SLAL:

111668981

XGL:	120804035(apeh) 120804543(zgc:136971)

HCQ:	109514146 109528691

SSCV:

125988408(zgc:136971) 125988895

BPEC:

110164337 110164529

MALB:

109968751 109970409

BSPL:

114858466(apeh) 114858578(zgc:136971)

SASA:

106566520(apeh) 106572275

STRU:

115166227 115166228 115166229 115166230 115168544(apeh) 115186142 115186143

OTW:	112215840(zgc:136971) 112222360(apeh)

OMY:	110492560(apeh) 110532073(zgc:136971)

OGO:	124031769(zgc:136971) 124045572(apeh)

ONE:	115101982(apeh) 115131960

SALP:

111966270 112075521(apeh)

SNH:	120059455(zgc:136971) 120061445(apeh)

CCLU:

121538145(zgc:136971) 121546083(apeh)

ELS:	105013991 105025272

SFM:	108925571 108925663

PKI:	111848764 111848792

AANG:

118207176(zgc:136971) 118208215

LOC:	102695461 102698619

PSPA:

121328622 121329185(apeh)

ARUT:

117411788 117411912 117962413 117964009

PSEX:

120540431(zgc:136971)

LCM:	102351784(APEH) 102352874

CMK:	103176730(apeh)

RTP:	109931683(apeh)

BFO:

118419257

BBEL:

109477623

CIN:

100176229

SCLV:

120341819 120342226 120342227

SPU:	577307 577370

APLC:

110984912

AJC:

117102103

BCOO:

119068143 119080617

AME:

727648

ACER:

107995414

ALAB:

122714028

BIM:

100747821

BBIF:

117217237

BVK:

117242890

BVAN:

117154982

BTER:

100649598

BPYO:

122574842

CCAL:

108627639

OBB:

114876260

MGEN:

117225915

NMEA:

116428763

CGIG:

122400667

SOC:

105200317

MPHA:

105837705

AEC:

105145648

ACEP:

105627926

PBAR:

105428617

VEM:

105563374

HST:

105184645

DQU:

106746095

CFO:

105256026

FEX:

115233882

LHU:

105674837

PGC:

109861327

OBO:

105280362

PCF:

106792095

PFUC:

122521167

VPS:

122632439

NVI:

100677815

TPRE:

106653231 106654148

MDL:

103578939

CGLO:

123275278

FAS:

105264922

DAM:

107043367

AGIF:

122852546

CCIN:

107272657

TCA:

659540

DPA:

109543917

SOY:	115878097 115881293

ATD:

109605806

CSET:

123312754

AGB:	108913882 108915347

LDC:

111512446

NVL:

108557532

APLN:

108740363

PPYR:

116175611 116175757

OTU:

111417399

BMOR:

101747101

BMAN:

114241309

MSEX:

115441512

DPL:	KGM_215038

BANY:

112048077

PMAC:

106718043

PPOT:

106106910

PXU:

106119135

PRAP:

111002001

PBX:

123711681

ZCE:

119832346

HAW:

110378249

HZE:

124633093

TNL:

113492196

SLIU:

111362214

OFU:

114362878

PXY:

105388523

API:	100167555 100168218 100169146 100573475

DNX:	107168239 107168241

AGS:

114130068

RMD:	113547880 113555547

BTAB:

109039741

DCI:

103517973

CLEC:

106663725

HHAL:

106685860 106685861

NLU:

111047695

PHU:	Phum_PHUM012930

FOC:

113208151

TPAL:

117639090

ZNE:

110830876

CSEC:

111865367

SGRE:

126362567

FCD:

110855503

DPX:	DAPPUDRAFT_302098

DMK:

116924668

DPZ:	124314738 124314764

PVM:

113816135

PJA:

122254285

PCHN:

125048271

HAME:

121855976

PCLA:

123760251

PTRU:

123517038

HAZT:

108668136

EAF:

111717925

LSM:	121120235 121130302

PPOI:

119113010

ISC:	IscW_ISCW021356

DSV:	119437361 119460997

RSAN:

119396508

RMP:

119174689

VDE:	111245343 111248348

VJA:	111262267 111268783

TUT:

107362082

DPTE:

113788532

CSCU:

111639859

SDM:

118200104

CEL:	CELE_R11E3.8(dpf-5)

CBR:	CBG_11649(Cbr-dpf-5)

BMY:	BM_BM5352(Bm5352)

LOA:	LOAG_17363

TSP:

Tsp_03275

HRO:	HELRODRAFT_193470

LGI:	LOTGIDRAFT_201138

BGT:

106054054

GAE:

121367420

HRF:

124118542

HRJ:

124276865

CRG:	105317255 105329391

MYI:

110448837

PMAX:

117331697

MCAF:

127730396

MMER:

123530387

DPOL:

127880940

OBI:

106869032

OSN:

115210423

LAK:

106176014

SMM:	Smp_211050

SHX:	MS3_00007922(XRCC6)

EGL:

EGR_01472

NVE:	116609737 116609738 5512276

EPA:

110245204

ATEN:

116301430

ADF:	107343892 107346701

AMIL:

114947681 114947682 114947683

PDAM:

113664354

SPIS:

111335762

XEN:	124435641 124435642

HMG:

100199643

TAD:	TRIADDRAFT_24080

AQU:

100637042

ATH:	AT4G14570(AARE)

ALY:

9304349

CRB:	17874238 17878877

CSAT:

104723745 104732186 109124643

EUS:	EUTSA_v10024477mg

BRP:	103833453 103868336 103872033

BNA:	106349067 106360606 106401462 106406252 106415233

BOE:	106300842 106312247 106331151

RSZ:	108818380 108820831 108822763

THJ:

104822254

CPAP:

110825053

CIT:

102610646

CIC:	CICLE_v10004325mg

PVY:	116113966 116130824

MINC:

123210019 123219984

TCC:

18603106

GRA:

105800572

GHI:	107928568 107929144

GAB:

108473735

DZI:

111306676

EGR:

104456143

GMX:	100803600 100812681

GSJ:	114372112 114372179

PVU:	PHAVU_007G279900g PHAVU_007G280000g

VRA:	106769975 106772197

VAR:	108324241 108326490

VUN:	114189572 114189573

CCAJ:

109789595

APRC:

113870811 113872585

MTR:	11420124 11429366 25482911

CAM:

101502347

PSAT:

127097767 127097769

LJA:	Lj0g3v0015409.1(Lj0g3v0015409.1)

ADU:	107473606 107474841

AIP:	107625545 107645368

AHF:	112719931 112727726 112747534 112793110

LANG:

109325642 109352404

FVE:	101311565 101312103

RCN:	112198192 112201036

PPER:

18791115

PMUM:

103323948 103323950

PAVI:

110763562 110763564

PDUL:

117623090 117623114

MDM:	103416877 103442764

PXB:	103929772 103940576

ZJU:

107413108

MNT:	112095240 21393572

CSV:

101207866

CMO:

103491214

BHJ:

120067711

MCHA:

111009690

CMAX:

111499357

CMOS:

111460152

CPEP:

111806948

RCU:

8280187

JCU:

105632075

HBR:	110644620 110673285

MESC:

110611758

POP:

7473443

PEU:

105127747

PALZ:

118056683 118056707

JRE:

108989467

CILL:

122277781

QSU:

112001064

QLO:

115994929

TWL:

120015547

VVI:

100242540

VRI:

117914513

SLY:

101258623

SPEN:

107015056

SOT:

102595350

SSTN:

125858621

CANN:

107868464

NTA:	107782506 107811002

NSY:	104223836 104223837 104224252

NTO:	104096282 104096283 104121104

NAU:

109211905

INI:

109175906

ITR:	116001797 116001798

SIND:

105171627

OEU:

111393713

EGT:

105962901

SSPL:

121774181 121776064 121776171 121777697

APAN:

127249112

HAN:

110894440

ECAD:

122604689 122604718

LSV:	111910042 111910043

CCAV:

112501670

DCR:

108199676

CSIN:

114323350

BVG:	104895819 104903937

SOE:

110792382

CQI:	110686144 110718543 110722172 110739376

NNU:

104589648

MING:

122061651 122091191

TSS:	122638728 122638735 122642666

PSOM:

113350022

OSA:	4348626 4348628

DOSA:

Os10t0415800-01(Os10g0415800)

OBR:	102717281 102717554

OGL:	127752959 127752960

BDI:	100824207 100837577

ATS:	109763464 109786579

TDC:	119285881 119319613 119322638 119348890

TAES:

123061550 123131769 123135638 123138938 123143221 123182530

TUA:	125523441 125527859

LPER:

127309064 127344932

SBI:	110433074 8064918

ZMA:	100281428 100285393

SITA:

101784436 101785236

SVS:	117836161 117836162

PVIR:

120651052 120688237 120693407

PHAI:

112874717 112875312

PDA:

103711615

EGU:

105053423

MUS:

103969438

ZOF:	121977707 122036726

DCT:

110114561

PEQ:

110032413

AOF:	109820121 109822988

NCOL:

116255857

ATR:

18429541

SMO:	SELMODRAFT_168303

PPP:	112278092 112284190

CRE:	CHLRE_05g241650v5

VCN:	VOLCADRAFT_100173

CSL:	COCSUDRAFT_43626

CVR:	CHLNCDRAFT_134052

MPP:	MICPUCDRAFT_53049

CME:	CYME_CMT228C

GSL:	Gasu_23490

CCP:	CHC_T00001116001

LBC:	LACBIDRAFT_319100

CCI:	CC1G_15336

MPR:	MPER_10083

SCM:	SCHCO_02590080(SCHCODRAFT_02590080) SCHCO_02639825(SCHCODRAFT_02639825)

PGR:	PGTG_02024

DDI:	DDB_G0292456(apeh)

DPP:	DICPUDRAFT_51071

DFA:	DFA_09138(apeh)

CPV:

cgd5_4370

CHO:	Chro.50405

TGO:	TGME49_246800

PTM:	GSPATT00018893001

PTI:	PHATRDRAFT_13777

FCY:	FRACYDRAFT_179058

TPS:	THAPSDRAFT_262750

PIF:	PITG_07354

PSOJ:

PHYSODRAFT_510613

GTT:	GUITHDRAFT_119792

NGR:	NAEGRDRAFT_74773

TVA:	TVAG_2v0982040

FSG:	LQ771_14910

LFL:	IM816_08045

ILO:

IL0039

CCS:	CCNA_02065

BCE:

BC0192

OJD:	NP439_13775

TTH:

TT_C1241

FNU:

FN1128

SRU:

SRU_2034

APE:	APE_1547.1 APE_1832 APE_2290.1

» show all

Reference

1 [PMID:1137989]

Authors

Tsunasawa S, Narita K, Ogata K.

Title

Purification and properties of acylamino acid-releasing enzyme from rat liver.

Journal

J Biochem (Tokyo) 77:89-102 (1975)

Reference

2 [PMID:477668]

Authors

Unger T, Nagelschmidt M, Struck H.

Title

N-Acetylaminoacyl-p-nitranilidase from human placenta. Purification and some properties.

Journal

Eur J Biochem 97:205-11 (1979)
DOI:10.1111/j.1432-1033.1979.tb13104.x

Reference

3 [PMID:3305492]

Authors

Kobayashi K, Smith JA.

Title

Acyl-peptide hydrolase from rat liver. Characterization of enzyme reaction.

Journal

J Biol Chem 262:11435-45 (1987)

Sequence

[rno:24206]

Other DBs

ExplorEnz - The Enzyme Database:

3.4.19.1

IUBMB Enzyme Nomenclature:

3.4.19.1

ExPASy - ENZYME nomenclature database:

3.4.19.1

BRENDA, the Enzyme Database:

3.4.19.1

CAS:	73562-30-8

DBGET integrated database retrieval system