KEGG   ENZYME: 3.4.19.16Help
Entry
EC 3.4.19.16                Enzyme                                 

Name
glucosinolate gamma-glutamyl hydrolase;
GGP1 (gene name);
GGP3 (gene name)
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Omega peptidases
BRITE hierarchy
Reaction(IUBMB)
(1) an (E)-1-(glutathion-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine + H2O = an (E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine + L-glutamate [RN:R11792];
(2) (E)-1-(glutathion-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine + H2O = (E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine + L-glutamate [RN:R11793];
(3) (glutathion-S-yl)(1H-indol-3-yl)acetonitrile + H2O = (L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile + L-glutamate [RN:R11794];
(4) (Z)-1-(glutathion-S-yl)-N-hydroxy-2-phenylethan-1-imine + H2O = (Z)-1-(L-cysteinyglycin-S-yl)-N-hydroxy-2-phenylethan-1-imine + L-glutamate [RN:R11795]
Reaction(KEGG)
Substrate
(E)-1-(glutathion-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine;
H2O [CPD:C00001];
(E)-1-(glutathion-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine;
(glutathion-S-yl)(1H-indol-3-yl)acetonitrile;
(Z)-1-(glutathion-S-yl)-N-hydroxy-2-phenylethan-1-imine
Product
(E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine;
L-glutamate [CPD:C00025];
(E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine;
(L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile;
(Z)-1-(L-cysteinyglycin-S-yl)-N-hydroxy-2-phenylethan-1-imine
Comment
This enzyme, characterized from the plant Arabidopsis thaliana, participates in the biosynthesis of the plant defense compounds glucosinolates and camalexin. It is the only known plant enzyme capable of hydrolysing the gamma-glutamyl residue of glutathione in the cytosol.
History
EC 3.4.19.16 created 2017
Orthology
K22314  glucosinolate gamma-glutamyl hydrolase
Genes
EPA: 110235103 110236718 110255075
ADF: 107348401
ATH: AT2G23960 AT2G23970 AT4G30530(GGP1) AT4G30550(GGP3)
ALY: ARALYDRAFT_328661 ARALYDRAFT_353756 ARALYDRAFT_491698 ARALYDRAFT_667648 ARALYDRAFT_920239
CRB: 17878381 17878489 17880012 17880488 17890112
CSAT: 104702915 104713836 104717139 104717141 104717142 104721790 104721793 104721794 104730255 104730257 104730258 104752025 104774366
EUS: EUTSA_v10000299mg EUTSA_v10000640mg EUTSA_v10026067mg EUTSA_v10026083mg EUTSA_v10027000mg
BRP: 103834667 103834668 103842392 103842394 103852592 103861974
BNA: 106363694 106365158 106365159 106365160 106392894 106400381 106408952 106414873 106416083 106424103 106424211 106453017 111198271
BOE: 106306103 106306562 106310578 106312467 106329223 106329266
CPAP: 110819018
LJA: Lj4g3v0386190.1(Lj4g3v0386190.1) Lj4g3v2016180.1(Lj4g3v2016180.1) Lj6g3v2192310.1(Lj6g3v2192310.1)
DOSA: Os02t0179200-01(Os02g0179200) Os03t0685300-01(Os03g0685300) Os09t0424200-01(Os09g0424200)
ATS: 109745571(LOC109745571) 109750656(LOC109750656) 109750657(LOC109750657) 109750658(LOC109750658) 109781866(LOC109781866)
PPP: 112285930
ABP: AGABI1DRAFT130281(AGABI1DRAFT_130281)
ABV: AGABI2DRAFT121902(AGABI2DRAFT_121902)
DFA: DFA_02717
HYB: Q5W_04450
 » show all
Taxonomy
Reference
1  [PMID:21712415]
  Authors
Geu-Flores F, Moldrup ME, Bottcher C, Olsen CE, Scheel D, Halkier BA
  Title
Cytosolic gamma-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis.
  Journal
Plant Cell 23:2456-69 (2011)
DOI:10.1105/tpc.111.083998
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.4.19.16
IUBMB Enzyme Nomenclature: 3.4.19.16
ExPASy - ENZYME nomenclature database: 3.4.19.16
BRENDA, the Enzyme Database: 3.4.19.16

DBGET integrated database retrieval system