KEGG ENZYME: 3.4.21.1

ENZYME: 3.4.21.1

Entry

EC 3.4.21.1 Enzyme

Name

chymotrypsin;
chymotrypsins A and B;
alpha-chymar ophth;
avazyme;
chymar;
chymotest;
enzeon;
quimar;
quimotrase;
alpha-chymar;
alpha-chymotrypsin A;
alpha-chymotrypsin

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Preferential cleavage: Tyr!, Trp!, Phe!, Leu!

Comment

Chymotrypsin A is formed from cattle and pig chymotrypsinogen A, several iso-forms being produced according to the number of bonds hydrolysed in the precursor. Chymotrypsin B (formerly listed as EC 3.4.4.6), formed from chymotrypsinogen B, is homologous with chymotrypsin A. Enzymes with specificity similar to that of chymotrypsins A and B have been isolated from many species. In peptidase family S1 (trypsin family)

History

EC 3.4.21.1 created 1961 as EC 3.4.4.5 and EC 3.4.4.6, transferred 1972 to EC 3.4.21.1

Orthology

K01310

chymotrypsin

Genes

HSA:	1504(CTRB1) 440387(CTRB2)

PTR:	736467(CTRB2) 736783

PPS:	100979112(CTRB2) 100979449(CTRB1)

GGO:	101134917 101141122

PON:	100434840 100447221

NLE:

115836698

MCC:	711100(CTRB2) 713851

MCF:	102146658(CTRB2) 102147021(CTRB1)

CSAB:

103233307 103233308

RRO:	104677837(CTRB2) 104677859

RBB:	108525819 108527423

CJC:	100391196(CTRB1) 100401959

SBQ:	101041600 101041923

MMU:	66473(Ctrb1)

MCAL:

110300407

RNO:	24291(Ctrb1)

MUN:

110554750

CGE:

100769139

NGI:

103725666

HGL:	101710293 101719459(Ctrb1)

CCAN:

109684024(Ctrb2)

OCU:	100341936 100342191

TUP:	102489237(CTRB1) 102489643

CFA:	479649 479650(CTRB2) 610373

VVP:	112915848 112915850

AML:	100478694 100479949

UMR:	103656130 103662066 103662113 103662114

UAH:	113251738 113251739 113252497 113257758

ORO:	101364050(CTRB1) 101367705 101367998

ELK:	111152797 111152908 111153069

FCA:	101091168 101096125 101096371 111561341

PTG:	102956672 102962235(CTRB1) 102962532

PPAD:

109245696 109245697 109245699 109268789

AJU:	106977417 106989659 106989660 106989661

BTA:	100848132 504241(CTRB1) 618826

BOM:	102265599(CTRB1) 102286947 102287233 106700787

BIU:	109571602 109572005 109572232

BBUB:

102391462 102398934 112580189

CHX:	102173875 102174148 102176156 108636028

OAS:	101116249 101117443 114112429 114112430 114114039 114117780 114117802

SSC:	100621642(CTRB2) 100621820 100739735

CFR:	102521639 102521880 102524246

CDK:	105104323 105105250 105106186

BACU:

103002465 103002741

LVE:	103078018(CTRB1) 103078294 103078577

OOR:	101283416 101285785 101286030

DLE:	111179508 111179510(CTRB1)

PCAD:

102975993 102976269 102976557

ECB:	100146723 102150576

EPZ:	103553045 103553048

EAI:	106833713 106836125

MYB:	102246722(CTRB1) 102256565

MYD:	102752429(CTRB1)

MNA:	107525597 107525608

HAI:	109384817 109384818

DRO:	112300682(CTRB1)

PALE:

102897343

RAY:	107502674 107502677

MJV:	108387272 108396172 108396173

LAV:

100672744

TMU:	101346609 101352410

MDO:

100023625

SHR:	100915387 100926099 105749186 105749238

PCW:	110192485 110192499

OAA:	100082597 100082620 107547255

GGA:	112529919 431235(CTRB2)

MGP:

100544825

CJO:	107319077 107319196

NMEL:

110404411(CTRB1)

APLA:

101790190

ACYG:

106033168

TGU:	100221838 105758698

LSR:

110476244

SCAN:

103816662

FAB:	101809681 101819337

PHI:	102099273 102099582(CTRB1) 102109513

PMAJ:

107209878 107209879

ETL:	114069814 114069816

FPG:	101924388(CTRB1)

CLV:	102091024 106146127

NNI:

104016383

ACUN:

113484313 113484551 113488394

PADL:

103918119

ASN:	102376101 102376351

AMJ:	102565040(CTRB2) 102566903 109283535

CMY:	102937055(CTRB1) 102938194

CPIC:

101937265(CTRB1) 101951625

ACS:	100565667(ctrb2)

PBI:	103054169 103055448

PMUR:

107287370 107287371

TSR:

106539016

PMUA:

114602911 114603013

GJA:	107107885 107107897

XLA:	108713630 108715072 379495(ctrl.L) 379607(ctrb1.L) 444360(ctrb2.L)

XTR:	100489516 101733231 394984(ctrb1) 496968(ctrb2) 548358(ctrl)

NPR:	108789591 108796300 108796301 108796302

DRE:	322451(ctrb1) 550419(zgc:112160) 562139 692313(zgc:136461)

SRX:	107736419 107740462 107740487 107740502 107755026 107755043 107755119 107755120

SANH:

107657100 107696673 107696674 107696676

SGH:	107548936 107548953 107548954 107563459 107563461 107563495

CCAR:

109051619 109051621 109051655 109061241

IPU:

108274430

PHYP:

113529515 113540780 113540809

AMEX:

103027485 103028134 103028442 103033388 103033709

EEE:	113571848 113582722

TRU:	101074356 101074582 101077681 101077910 105417243 115252056

TNG:	GSTEN00019361G001 GSTEN00019362G001 GSTEN00019363G001

LCO:

104938940

MZE:	101469324 101483816 105941050

ONL:	100691584 100708221 102079176

OLA:	101170410 105356289

XMA:	102231986 111608451

XCO:	114143729 114143730

PRET:

103460505

CVG:	107096265 107096266

NFU:	107381521 107381578

KMR:

108248653

ALIM:

106532477

AOCE:

111567950 111586722

CSEM:

103378636 103378813 103378814 103378818 103378820

POV:

109624876

LCF:	108894207 108894209

SDU:	111225511 111225519

SLAL:

111649677 111652972 111654878 111667238 111667239

HCQ:	109529759 109529808

BPEC:

110161934 110161954 110161965

MALB:

109961663

SASA:

100196064(ctrb) 106576578 106588135 106591457 106592898 106609703

OTW:	112217114 112223934

SALP:

112072423 112077794

ELS:	105020421 105025470 105025471

SFM:	108926238 108926269 114910966

PKI:

111843547

LCM:	102353013 102363505

CMK:	103176139 103176140 103176141 103176142 103176187 103176191

RTP:	109915217 109915218 109935179

BFO:	118403996 118405025 118405040 118405176 118405177 118411574 118426942 118427491 118427714 118427831

CIN:	100178009 100181838 100181939 101242836

SPU:

105437658

APLC:

110978230

SKO:	100374383 100375731 100377421 102805473 102807257

DME:	Dmel_CG18179(CG18179) Dmel_CG6298(Jon74E)

DER:	6541630 6545101 6545296 6554094

DSI:	Dsimw501_GD12397(Dsim_GD12397) Dsimw501_GD13920(Dsim_GD13920) Dsimw501_GD13923(Dsim_GD13923) Dsimw501_GD18805(Dsim_GD18805) Dsimw501_GD22875(Dsim_GD22875) Dsimw501_GD28182(Dsim_GD28182)

DYA:	Dyak_GE15231 Dyak_GE16217 Dyak_GE20517 Dyak_GE21525 Dyak_GE22138 Dyak_GE26165

DSR:	110184693 110184717 110184729

DPO:	Dpse_GA19610 Dpse_GA19627 Dpse_GA24722

DPE:	113564919 6601482 6603255

DMN:	108152481 108152482 108153879 108165368

DWI:	26529398 26529761 6639493 6643904

DGR:	Dgri_GH11152 Dgri_GH16447 Dgri_GH17316

DMO:	Dmoj_GI13255 Dmoj_GI13257 Dmoj_GI13708 Dmoj_GI22460

DAZ:	108613159 108613179 108621027

DNV:	108650978 108660193

DHE:	111592766 111603674 111604592

MDE:	101897780 101898109 101898879 101898960

LCQ:	111676806 111676807 111676808 111676819 111676821 111676845 111676848 111676849 111683743 111684489 111689914 111690945

AAG:	5569033 5570259 5570261 5570262 5570265 5570266 5570270

CQU:	CpipJ_CPIJ002098 CpipJ_CPIJ002127 CpipJ_CPIJ002128 CpipJ_CPIJ002133 CpipJ_CPIJ002134 CpipJ_CPIJ002135 CpipJ_CPIJ002156 CpipJ_CPIJ007384 CpipJ_CPIJ011433 CpipJ_CPIJ016102 CpipJ_CPIJ017990

MPHA:

AEC:

ACEP:

DQU:

LHU:

OBO:

NVI:	100121542(SP102) 100124045

CSOL:

105359087 105365785 105365793 105365818

TCA:	103313392(H152) 656549(H110)

ATD:

109602633

NVL:	108562331 108563243

PMAC:

106714679

PRAP:

110992965 111000370 111003156

HAW:

110373620

TNL:

113494420

CLEC:

106664788 106673453

PHU:	Phum_PHUM440020 Phum_PHUM565430 Phum_PHUM610350

FCD:	110845616 110850452 110860066

DPX:	DAPPUDRAFT_105505 DAPPUDRAFT_111683 DAPPUDRAFT_246339 DAPPUDRAFT_301538 DAPPUDRAFT_316896 DAPPUDRAFT_320584 DAPPUDRAFT_335851 DAPPUDRAFT_347826(TRY1)

PVM:	113803370 113817021 113818967

ISC:	IscW_ISCW012738 IscW_ISCW014005

TUT:	107371103 107371919

NAI:	NECAME_08402 NECAME_11866

TSP:	Tsp_00339 Tsp_03012 Tsp_03013 Tsp_03015 Tsp_09782

HRO:	HELRODRAFT_170197

LGI:	LOTGIDRAFT_159254 LOTGIDRAFT_226539 LOTGIDRAFT_229657

CRG:

105343824

MYI:	110440267 110449410 110456811 110459661

OBI:	106870942 106875202 106880032

LAK:

112042437

NVE:	116607154 5501292 5506183 5510463 5512616 5514114 5516191 5519306 5519323

EPA:	110236060 110236291 110236966 110236968 110237783 110242679 110244488 110245130 110246458 110253045 110253053 110253113 110254988

AMIL:

114963331 114963336

PDAM:

113665228 113665269 113665301 113674910 113681979 113682055

SPIS:

111322500 111341127 111341787 111341789 111341790 111341802

DGT:

114521678

HMG:

100213885

FCY:	FRACYDRAFT_244069

PIF:	PITG_02700 PITG_02704 PITG_02707 PITG_13655 PITG_13671

PSOJ:

PHYSODRAFT_310065 PHYSODRAFT_341711 PHYSODRAFT_341752 PHYSODRAFT_470322 PHYSODRAFT_481652 PHYSODRAFT_531672

SACZ:

AOT14_04420

MICT:

FIU95_18505

» show all

Reference

Authors

Wilcox, P.E.

Title

Chymotrypsinogens - chymotrypsins.

Journal

Methods Enzymol 19:64-108 (1970)

Reference

Authors

Blow, D.M.

Title

Structure and mechanism of chymotrypsin.

Journal

Acc Chem Res 9:145-152 (1976)

Reference

3 [PMID:6768556]

Authors

Bauer CA.

Title

Active centers of alpha-chymotrypsin and of Streptomyces griseus proteases 1 and 3. S2-P2 enzyme-substrate interactions.

Journal

Eur J Biochem 105:565-70 (1980)
DOI:10.1111/j.1432-1033.1980.tb04533.x

Reference

Authors

Polgar, L.

Title

Structure and function of serine proteases.

Journal

In: Neuberger, A. and Brocklehurst, K. (Eds.), New Comprehensive Biochemistry: Hydrolytic Enzymes, vol. 16, Elsevier, Amsterdam, 1987, p. 159-200.

Reference

5 [PMID:2917002]

Authors

Tomita N, Izumoto Y, Horii A, Doi S, Yokouchi H, Ogawa M, Mori T, Matsubara K

Title

Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA.

Journal

Biochem Biophys Res Commun 158:569-75 (1989)
DOI:10.1016/s0006-291x(89)80087-7

Sequence

[hsa:440387 1504]

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.1

IUBMB Enzyme Nomenclature:

3.4.21.1

ExPASy - ENZYME nomenclature database:

3.4.21.1

BRENDA, the Enzyme Database:

3.4.21.1

CAS:

9004-07-3

DBGET integrated database retrieval system