KEGG   ENZYME: 3.4.21.1
Entry
EC 3.4.21.1                 Enzyme                                 
Name
chymotrypsin;
chymotrypsins A and B;
alpha-chymar ophth;
avazyme;
chymar;
chymotest;
enzeon;
quimar;
quimotrase;
alpha-chymar;
alpha-chymotrypsin A;
alpha-chymotrypsin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Preferential cleavage: Tyr!, Trp!, Phe!, Leu!
Comment
Chymotrypsin A is formed from cattle and pig chymotrypsinogen A, several iso-forms being produced according to the number of bonds hydrolysed in the precursor. Chymotrypsin B (formerly listed as EC 3.4.4.6), formed from chymotrypsinogen B, is homologous with chymotrypsin A. Enzymes with specificity similar to that of chymotrypsins A and B have been isolated from many species. In peptidase family S1 (trypsin family)
History
EC 3.4.21.1 created 1961 as EC 3.4.4.5 and EC 3.4.4.6, transferred 1972 to EC 3.4.21.1
Orthology
K01310  chymotrypsin
Genes
HSA1504(CTRB1) 440387(CTRB2)
PTR736467(CTRB2) 736783
PPS100979112 100979449
GGO101134917 101141122
PON100434840 100447221
NLE115836698
HMH116470816
MCC711100(CTRB2) 713851
MCF102146658(CTRB2) 102147021
MTHB126943760
MNI105491273 105491274(CTRB2)
CSAB103233307 103233308
CATY105572829(CTRB2) 105572907
PANU101013214(CTRB2) 116268652
TGE112613840(CTRB2) 112613841
MLEU105537239(CTRB2) 105537240
RRO104677837(CTRB2) 104677859
RBB108525819 108527423
TFN117088056(CTRB2)
PTEH111536153
CANG105514569
CJC100391196(CTRB1) 100401959
SBQ101041600 101041923
CIMI108305731 108305801
CSYR103251607 103270188
MMUR105875474 105875475(CTRB1)
LCAT123625430
PCOQ105808007
OGA100956077 100956389(CTRB1)
MMU66473(Ctrb1)
MCAL110300407
RNO24291(Ctrb1)
MCOC116068047
ANU117723190
MUN110554750
CGE100769139
MAUA101825831
PROB127234689
PLEU114709116 114709117
MORG121447482
MFOT126502860
AAMP119802244
NGI103725666
HGL101710293 101719459(Ctrb1)
CPOC100714036 100714680 100721715
CCAN109684024(Ctrb2)
DORD105982905 105982967 105982968 105983093 105983094 106002316
DSP122126162 122126209 122126210 122126211 122126212
PLOP125357871 125357872 125357873 125357874 125358182
NCAR124967034 124967339
MMMA107151090 125613095
OCU100341936 100342191
OPI101521949 101522298
TUP102489237(CTRB1) 102489643
GVR103582533 103582534
CFA479649 479650(CTRB2)
CLUD112646653 112646655 112646656(CTRB1)
VVP112915848 112915850
VLG121499706 121500658 121500961
NPO129497224(CTRB1) 129497225 129497655 129505499
AML100478694 100479949
UMR103656130 103662066(CTRB1) 103662113 103662114
UAH113251738 113251739 113252497 113257758
UAR123791312 123791411
ELK111152797 111152908 111153069
LLV125082280 125089503 125089504 125089548
MPUF101691590 101691591
MNP132008800 132009000 132009001
MLK131817947 131818054 131818063 131818127 131818713
NVS122911479 122911487 122913743 122913744
ORO101364050(CTRB1) 101367705 101367998
EJU114201155(CTRB1) 114201443 114216597
ZCA113936039(CTRB1) 113936409
MLX118023413 118023565
NSU110583368 110593944 110594215
LWW102746114 102746413 102747894
FCA101091168 101096125 101096371
PYU121009799 121009800 121024272
PCOO112857202 112857232
PBG122494470 122494472 122494473
PVIV125153028 125153029 125153191
LRUF124510768 124511904 124511909
PTG102956672 102962235(CTRB1) 102962532
PPAD109245696 109245697 109245699 109268789
PUC125916431 125916665 125916666 125918921
AJU106989659 106989660 106989661
HHV120248187 120248190 120248195(CTRB1)
BTA100848132 504241(CTRB2) 618826
BOM102265599(CTRB1) 102286947 102287233 106700787
BIU109571602 109572005 109572232
BBUB102391462 102398934 112580189
BBIS104996478 104996479 104996480
CHX102173875 102174148 102176156 108636028
OAS101116249 101117443 114112429
BTAX128062970 128062971 128063404
ODA120861142 120872853 120872854 120873342
CCAD122420743 122421114 122421332
MBEZ129546278 129546279 129547367
SSC100621642(CTRB2) 100621820 100739735
CFR102521639 102521880 102524246
CBAI105078637 105078642 105079785
CDK105104323 105105250 105106186
VPC102525791 102533673 116281932
BACU103002465 103002741
BMUS118885186 118885308 118885609 118902070
LVE103078018(CTRB1) 103078294 103078577
OOR101283416 101285785 101286030
DLE111179508 111179510(CTRB1)
PCAD102975993 102976269 102976557
PSIU116743430(CTRB2) 116744283 116744761
NASI112393345 112393425(CTRB2) 112393460
ECB100146723 102150576
EPZ103553045 103553048
EAI106836125 123275589
MYB102246722(CTRB1) 102256565
MYD102752429(CTRB1)
MMYO118674129 118674130
MLF102438386(CTRB1)
PKL118720506 118720507
EFUS103284371 103284372
MNA107525597 107525608
DRO112300682(CTRB1)
SHON118999277 118999278
AJM119048943 119048944
PDIC114488303 114488329
PHAS123806074 123806075(CTRB2)
MMF118639088 118639268
PPAM129075053 129075055
HAI109384817 109384818
RFQ117034905 117035023
PALE102897343
PGIG120617367 120617390
PVP105305376 105311637
RAY107502674 107502677
MJV108396172 108396173
TOD119249080 119249081 119249082 119249085 119250308 119250309 119250310 119250311 119250312 119250314 119250315 119250316 119250317 119250318 119250319 119250362 119250363 119250364 119250366 119250368 119250369 119250371 119250373 119250374
SARA101557385 101558657 101559191
SETR126027438 126027439 126027440
LAV100672744
TMU101346609 101352410
ETF101643293 101650541
DNM101428502 101429676
MDO100023625
GAS123233219 123254716
SHR100915387 100926099 105749186 105749238
AFZ127546401 127547687 127547688 127547689 127547690 127547692
PCW110192485 110192499
OAA100082597 100082620 107547255
GGA112529919 431235(CTRB2)
MGP100544825
CJO107319077 107319196
TPAI128081747 128081749
LMUT125699077 125699078
NMEL110404411(CTRB1)
APLA101790190 119718180
ACYG106033168
CATA118246426 118246450
AFUL116494143 116494172
TGU100221838 105758698
LSR110476244
SCAN103816662
PMOA120508034 120508166
OTC121334000 121334001
PRUF121348958
FAB101809681 101819337
OMA130257780 130257781
PHI102099273 102099582(CTRB1) 102109513
PMAJ107209878 107209879
CCW120410610 120410611
CBRC103620291
ETL114069814 114069816
ZAB102066970
ZLE135452677 135452765
ACHL103810088
SVG106864686
MMEA130572747
HRT120757793 120757810
FPG101924388(CTRB1)
CLV102091024 106146127
NNI104016383
PCRI104029176
ACUN113484313 113484551 113488394
TALA116963884 122153886
PADL103918119
AFOR103895843
ACHC115345873
HLE104842148(CTRB1)
AGEN126041202 126041203
GCL127021094 127021095
MUI104541905
ACAR104532764
RTD128908990 128909364
CUCA104054242
AROW112978193
NPD112943197 112943263
TGT104573898(CTRB1)
ASN102376101 102376351
AMJ102565040(CTRB2) 102566903 109283535
CPOO109310777 109310778
GGN109289488 109289489
CMY102937055 102938194 119563687
CCAY125620877 125620880 125620882
DCC119841038 119841039 119841178
CPIC101937265 101951625
TST117886625 117886751
CABI116823742 116823743 116831928
MRV120384610 120384612
ACS100565667(ctrb2)
ASAO132777433 132783302 132783314
SUND121937507 121937508
PBI103054169 103055448
PMUR107287370 107287371
CTIG120312672 120312674
TSR106539016
PGUT117670329 117670342
APRI131184301 131184302
PTEX113445325 113445327
NSS113420275 113420320
VKO123025330 123025430
PMUA114602911 114603013
PRAF128419020 128420397
ZVI118077563 118078015 118078073 118086454 118087759 118087770
HCG128334443 128334445
GJA107107885 107107897
STOW125443546 125443547
EMC129344220 129344338
XLA108713630 108713631 108713635 108715072 121402981 379495(ctrl.L) 379607(ctrb1.L) 444360(ctrb2.L)
XTR100489516 101733231 394984(ctrb1) 496968(ctrb2) 548358(ctrl)
NPR108789591 108796300 108796301 108796302
RTEM120917632 120920539 120920614 120920743 120920803 120920873 120920925 120920985 120921036 120921107 120921178 120921279 120921578 120921722 120921806 120921888 120925105
BBUF120980059 120980060 120980403 120980404 120980405 120980406 120980442 120980483
BGAR122920869 122920933 122920940 122920950 122920952 122920953 122920954 122920986
MUO115471220 115471311(CTRB2)
GSH117359722
DRE322451(ctrb.1) 550419(ctrb.3) 562139 692313(ctrb.2)
SRX107740462 107740487 107740502 107755026 107755043 107755119 107755120
SANH107657100 107696673 107696674 107696676
SGH107548936 107548953 107548954 107563459 107563461 107563495
CCAR109051619 109051621 109051655 109061241
CAUA113051482 113051484 113051485
CGIB128016874 128016875 128016876
PTET122348049(ctrb.3) 122348059
LROH127168978(ctrb.3) 127168979 127168980
OMC131543847 131544532(ctrb.3)
PPRM120490983 120491067
RKG130099591(ctrb.3) 130099592 130099594
MAMB125264819(ctrb.3) 125264820 125264821 125264822
CIDE127515588 127516065 127516066 127516067
MASI127416199 127416206 127448497
TROS130556937 130557024 130562737(ctrb.3)
TDW130421036 130426745(ctrb.3) 130426753
MANU129444514(ctrb.3) 129444525 129444540 129444547
IPU108274430
IFU128618051
PHYP113529515 113540780 113540809
SMEO124379939 124391985
TFD113639185
TVC132838670 132838767 132857222
TRN134303949 134311066
AMEX103027485 103028134 103028442 103033388 103033709
CMAO118805043 118811778 118811783 118812722 118817452(zgc:112160) 118817453 118817631
EEE113571848 113582722
CHAR105899747 105899750 105899752 105899753
TRU101074356 101074582 101077681 101077910 105417243 115252056
TFS130520246 130520253 130520259 130520267 130520275
TNGGSTEN00019361G001 GSTEN00019362G001 GSTEN00019363G001
LCO104938940
TBEN117469922 117469923 117472325
CGOB115005529 115005530 115005531
PGEO117442245 117442247 117442248
GACU117538580 117538581
EMAC134863642 134863643
ELY117250541 117254852 117254859 117254867
EFO125900162 125900164 125900169
PLEP121965227 121965274
SLUC116062381 116062384 116062651 116063137
ECRA117943329 117956471
ESP116692255 116693104
PFLV114556195 114556306
PPUG119193986 119199085
AFB129107313 129107323
CLUM117726062 117750045 117750053
MSAM119888105 119890060 119890061 119918844 119918845
SCHU122879328 122879336
CUD121509782 121509846
ALAT119018163
MZE101469324 101483816 105941050
ONL100691584 100708221 102079176
OAU116323365 116323375
OLA101170410 105356289
OML112138615 112138616
CSAI133457378 133457386 133457393
XMA102231986 111608451
XCO114143729 114143730
PRET103460505
PFOR103139941
PLAI106943317
PMEI106912757
GAF122823237 122823244
PPRL129352219 129352220 129355812
CVG107096265 107096266
CTUL119797486 119797487 119797490
GMU124866634 124866685 124866689 124867538
NFU107381521 107381578
KMR108248653
ALIM106532477
NWH119424882 119427345(ctrb.3)
AOCE111567950 111586722
MCEP125005315(ctrb.3) 125005389 125005441 125005754 125005755
CSEM103378636 103378813 103378814 103378818 103378820
POV109624876
SSEN122772690 122772691
HHIP117752381 117752393
HSP118101767
PPLT128445474 128445478 128445484
SMAU118301994 118301998
LCF108894207 108894209
SDU111225511 111225519
SLAL111649677 111652972 111654878 111667238 111667239
XGL120791548 120795722
HCQ109529759 109529808
SBIA133497648 133497653
PEE133395262 133418295
PTAO133468692 133469132
BPEC110161934 110161954 110161965
MALB109961663
BSPL114852151 114852777
SJO128359689 128359846 128367623 128367624 128373783 128373878
SASA100196064(ctrb) 106576578 106588135 106591457 106592898 106609703
STRU115180599 115199165 115203535 115203555
OTW112217114 112224680
OMY110490652 110490653 110510477 110515682
OGO124018461 124022865 124035470
ONE115115067 115116672
OKI109877234 109886150 109886152
OKE118381150 127924347
SALP112072423 112077794
SNH120037099 120038018 120038019 120038020 120039840 120050120
CCLU121548411 121550293 123487278
ELS105020421 105025470 105025471
SFM108926238 108926269 114910966
PKI111843547
AANG118215490 118215496 118227062 118227085
LOC102693054 102693259
PSPA121325261 121325262 121325432
ARUT117424715 117424717 117425720
PSEX120535237
LCM102353013 102363505
CMK103176139 103176140 103176141 103176142 103176187 103176191
RTP109915217 109915218 109935179
CPLA122558472 122558473 122558685
HOC132823748 132823749 132823750
LERI129705097 129705098
PMRN116942137 116944030 116944031 116944032 116944033 116944035 116944078 116944079 116944080 116944094
LRJ133343829 133350749 133350750 133350790 133350791 133350792 133350793 133350794 133350804
BFO118403996 118405025 118405040 118405176 118405177 118411574 118426942 118427491 118427714 118427831
BBEL109463802 109472895 109473066 109473121 109484998 109485071 109485090
CIN100178009 100181838 100181939 101242836
SCLV120325525 120325756 120325759 120326175 120326176 120327009 120327287 120329401 120344373
SPU105437658
LPIC129270197 129275797 129282040
APLC110978230
SKO100374383 100375731 100377421 102805473 102807257
DMEDmel_CG18179(CG18179) Dmel_CG6298(Jon74E)
DER6541630 6545101 6545296 6554094
DSIDsimw501_GD12397(Dsim_GD12397) Dsimw501_GD13920(Dsim_GD13920) Dsimw501_GD13923(Dsim_GD13923) Dsimw501_GD18805(Dsim_GD18805) Dsimw501_GD22875(Dsim_GD22875) Dsimw501_GD28182(Dsim_GD28182)
DYADyak_GE15231 Dyak_GE16217 Dyak_GE20517 Dyak_GE21525 Dyak_GE22138 Dyak_GE26165
DSR110184693 110184717 110184729
DPO5495507 6900956
DPE113564919 6601482 6603255
DMN108152481 108152482 108153879 108165368
DWI26529398 26529761 6639493 6643904
DMODmoj_GI13255 Dmoj_GI13257 Dmoj_GI13708 Dmoj_GI22460
DAZ108613159 108613179 108621027
DNV108650978 108660193
DHE111592766 111603674 111604592
AOQ129236961 129240955 129241341 129242521 129242675
TDA119677099 119677103
MDE101897780 101898109 101898879 131801295
SCAC106095262
LCQ111676821 111676848 111683743 111690945
ECOE129941471
CLON129608976
AGA1269887 1273529 1276347 1276349 1276350 1276351 1276364 1277264 1281971 133391403 3290017 3290018 3290022 3290023 3290331 5667010 5667317 5667341 5667342 5667393
ACOZ120949018 120949091 120949540 120949878 120949907 120949929 120950859 120951382 120951383 120951930 120952128 120952255 120952509 120952801 120952861 120952862 120953077 120961380
AARA120894443 120897820 120905049
AMER121590962 121592286 121594454 121599647
ASTE118508822 118513112
AFUN125760719 125770075
AMOU128302877 128305182 128305186 128309506
AAG5570259 5570261 5570262 5570265 5570266 5570270
AALB109409740 109409741 109409742 109409744 109409751 109409753 109409755 109415845 109431992 109432002 109432034 109432055 109432077 109432112 115256233 115265379 115265380 115265384
ASUA134213977 134213978 134213979 134213983 134213984 134213985 134213988 134213989 134213996 134217605 134217606 134223284 134223285 134224246
CQUCpipJ_CPIJ002098 CpipJ_CPIJ002127 CpipJ_CPIJ002128 CpipJ_CPIJ002133 CpipJ_CPIJ002134 CpipJ_CPIJ002135 CpipJ_CPIJ002156 CpipJ_CPIJ007384 CpipJ_CPIJ011433 CpipJ_CPIJ016102 CpipJ_CPIJ017990
CPII120412702 120413246 120419348 120420019 120423018 120423020 120423023 120427246 120427610 120428388 120428409 120428410 120428411 120428413
MPHA114253974
ACEP105618623
DQU106748793
LHU105676589
NVI100121542(SP102) 100124045
CSOL105365785 105365793 105365818
TPRE106649092 106655906 106657807
AGIF122851136
VCAN122417094
NPT124213111
NFB124175528
NLO107224110
NVG124297674
TCA103313392(H152)
SOY115884698 115884706
DVV114326719
NVL108562331
APLN108736052
LSIN126978143
HAW110373620
TNL113494420
SLIU111355580 111356392
PGW126379731
LGLY125236778 125241329
CCRN123298715 123299689 123299690 123301545
CLEC106664788
HHAL106679425
HVI124354471
PHUPhum_PHUM610350
TPAL117647215
BROR134528620
DPXDAPPUDRAFT_111683 DAPPUDRAFT_246339 DAPPUDRAFT_301538 DAPPUDRAFT_316896 DAPPUDRAFT_320584 DAPPUDRAFT_335851 DAPPUDRAFT_347826(TRY1)
DMK116929074 116933805
PVM113817021 113818967
PJA122244516 122252972
PCHN125026990
MNZ135202058 135202730
HAZT108676848
EAF111698094 111698104 111701749 111704166
LSM121127609
TCF131879010
DSV119435821 119445571 119458952
RSAN119373425 119382902 119390733 119390734
RMP119165030 119169187 119169990 119182475
VDE111249093 111251113
VJA111258932 111269777
TUT107371103
DFR124499468
ABRU129961630 129961981
UDV129224815 129224901
PMEO129581565 129581695 129583081 129583092 129590685 129596710 129597165 129600472
NAINECAME_08402
TSPTsp_00339 Tsp_03012 Tsp_03013
LGILOTGIDRAFT_159254 LOTGIDRAFT_226539 LOTGIDRAFT_229657
PVUL126816411 126828011
BGT106061198 106063034 106063489 106077507
GAE121369457 121374728 121389079
HRF124113812 124116273 124127381
HRJ124256260 124256270 124260320 124260383 124260627 124264924 124272550
CRG105321691 105339174 105343824 117681657 117684283
CVN111120583 111122041 111122788 111126375 111126930
CANU128155378 128156072 128164731
OED125678145 125680494 130051658
MYI110440267 110449410 110456811 110459661
PMAX117330793 117339749 117343139
MCAF127711847 127712063
MMER123523040 123546027 123553447 123565868
RPHI132720485 132726156 132732317 132750790 132750791 132756971
DPOL127832147 127840411 127850598 127855670 127857619 127858490 127858759 127858760 127861520 127863856 127863857 127865734 127868024 127868531 127870356
OBI106870942 106880032
OSN115218084 115224312 115226866 118763693
LJP135475633 135475874 135475927 135481029 135481030
LAK112042437
LLON135483610 135484911 135487394 135490906 135491355
NVE116607154 5501292 5506183 5510463 5512616 5514114 5516191 5519306 5519323
EPA110236060 110236291 110236966 110236968 110237783 110242679 110244488 110245130 110246458 110253045 110253053 110253113 110254988
ATEN116287990 116292485 116293200 116300341 116307728 116307729 116307993 116307995 116308097 116308151 116308537
AMIL114963331 114963336
PDAM113665228 113665269 113665301 113674910 113681979 113682055
SPIS111322500 111341127 111341787 111341789 111341790 111341802
DGT114521678
HMG100213885
HSY130612255 130641072 130641073 130642116 130645489 130645537 130646307
PIFPITG_02700 PITG_02704 PITG_02707 PITG_13655 PITG_13671
PSOJPHYSODRAFT_310065 PHYSODRAFT_341711 PHYSODRAFT_341752 PHYSODRAFT_470322 PHYSODRAFT_481652 PHYSODRAFT_531672
 » show all
Reference
1
  Authors
Wilcox, P.E.
  Title
Chymotrypsinogens - chymotrypsins.
  Journal
Methods Enzymol 19:64-108 (1970)
Reference
2
  Authors
Blow, D.M.
  Title
Structure and mechanism of chymotrypsin.
  Journal
Acc Chem Res 9:145-152 (1976)
Reference
3  [PMID:6768556]
  Authors
Bauer CA.
  Title
Active centers of alpha-chymotrypsin and of Streptomyces griseus proteases 1 and 3. S2-P2 enzyme-substrate interactions.
  Journal
Eur J Biochem 105:565-70 (1980)
DOI:10.1111/j.1432-1033.1980.tb04533.x
Reference
4
  Authors
Polgar, L.
  Title
Structure and function of serine proteases.
  Journal
In: Neuberger, A. and Brocklehurst, K. (Eds.), New Comprehensive Biochemistry: Hydrolytic Enzymes, vol. 16, Elsevier, Amsterdam, 1987, p. 159-200.
Reference
5  [PMID:2917002]
  Authors
Tomita N, Izumoto Y, Horii A, Doi S, Yokouchi H, Ogawa M, Mori T, Matsubara K
  Title
Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA.
  Journal
Biochem Biophys Res Commun 158:569-75 (1989)
DOI:10.1016/s0006-291x(89)80087-7
  Sequence
[hsa:440387 1504]
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.1
IUBMB Enzyme Nomenclature: 3.4.21.1
ExPASy - ENZYME nomenclature database: 3.4.21.1
BRENDA, the Enzyme Database: 3.4.21.1
CAS: 9004-07-3

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