| Entry |
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| Name |
physarolisin;
Dictyostelium discoideum aspartic proteinase;
Dictyostelium discoideum aspartic proteinase E;
Physarum flavicomum aspartic proteinase;
Physarum polycephalum acid proteinase;
Physarum aspartic proteinase;
physaropepsin
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| Class |
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
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| Reaction(IUBMB) |
Milk clotting activity. Preferential cleavage of Gly8!Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19!Gly and Phe24!Phe. No action on Ac-Phe-Tyr(I)2.
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| Comment |
Belongs in peptidase family S53. From the slime mold Physarum polycephalum. Is not inhibited by pepstatin, but is blocked by methyl 2-diazoacetamidohexanoate. Closely similar enzymes are found in Dictyostelium discoideum and P. flavicomum. Formerly included in EC 3.4.23.6.
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| History |
EC 3.4.21.103 created 1992 as EC 3.4.23.27 (EC 3.4.23.6 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992), transferred 2003 to EC 3.4.21.103
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| Orthology |
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| Genes |
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| Reference |
1 |
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| Authors |
Henney, H.R. and Tavana, G. |
|---|
| Title |
Purification and some properties of an intracellular acid (carboxyl) proteinase from differentiating haploid cells of Physarum flavicomum. |
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| Journal |
Exp Mycol 6:161-170 (1982) |
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| Reference |
2 |
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| Authors |
Murakami-Murofushi, K., Hiratsuka, A. and Ohta, J. |
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| Title |
A novel acid protease from haploid amoebae of Physarum polycephalum, and its changes during mating and subsequent differentiation into diploid plasmodia. |
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| Journal |
Cell Struct Funct 9:311-315 (1984) |
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| Reference |
3 |
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| Authors |
North, M.J. and Whyte, A. |
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| Title |
Purification and characterization of two acid proteinases from Dictyostelium discoideum. |
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| Journal |
J Gen Microbiol 130:123-134 (1984) |
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| Reference |
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| Authors |
Wlodawer A, Li M, Gustchina A, Oyama H, Dunn BM, Oda K. |
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| Title |
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases. |
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| Journal |
Acta Biochim Pol 50:81-102 (2003) |
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| Reference |
|
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| Authors |
Nishii W, Ueki T, Miyashita R, Kojima M, Kim YT, Sasaki N, Murakami-Murofushi K, Takahashi K |
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| Title |
Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase. |
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| Journal |
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| Sequence |
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| Other DBs |
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