KEGG   ENZYME: 3.4.21.104
Entry
EC 3.4.21.104               Enzyme                                 
Name
mannan-binding lectin-associated serine protease-2;
MASP-2;
MASP2;
MBP-associated serine protease-2;
mannose-binding lectin-associated serine protease-2;
p100;
mannan-binding lectin-associated serine peptidase 2
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Selective cleavage after Arg223 in complement component C2 (-Ser-Leu-Gly-Arg!Lys-Ile-Gln-Ile) and after Arg76 in complement component C4 (-Gly-Leu-Gln-Arg!Ala-Leu-Glu-Ile)
Comment
Mannan-binding lectin (MBL) recognizes patterns of neutral carbohydrates, such as mannose and N-acetylglucosamine, on a wide range of microbial surfaces and is able to initiate activation of the lectin pathway of complement [7]. This enzyme displays C_overbar_1s_-like esterolytic activity (cf. EC 3.4.21.42, complement subcomponent C_overbar_1s_). It also cleaves C4 and C2 with efficiencies that are relatively higher than those of EC 3.4.21.42 [3]. Belongs in peptidase family S1A.
History
EC 3.4.21.104 created 2005
Orthology
K03993  mannan-binding lectin serine protease 2
Genes
HSA10747(MASP2)
PTR107974877(MASP2)
PPS100981899(MASP2)
GGO101126677(MASP2)
PON100458242(MASP2)
NLE100593253(MASP2)
MCC722714(MASP2)
CSAB103225618(MASP2)
CATY105580873(MASP2)
TGE112616993 112617028
RRO104654364(MASP2)
RBB108539381(MASP2)
TFN117093329(MASP2)
CJC100408774(MASP2)
SBQ101047723(MASP2)
CSYR103252233(MASP2)
MMUR105886046(MASP2)
OGA100957137(MASP2)
MMU17175(Masp2)
MCAL110292278(Masp2)
MPAH110323473(Masp2)
RNO64459(Masp2)
MCOC116097019(Masp2)
MUN110548141(Masp2)
CGE100756295 100770654
PLEU114709641(Masp2)
NGI103733193(Masp2)
HGL101696699(Masp2) 101706103
CPOC100725747(Masp2)
CCAN109689658(Masp2)
DORD105998488(Masp2)
DSP122121477(Masp2)
NCAR124991948
OPI101525865(MASP2)
TUP102488905(MASP2)
CFA487447(MASP2)
VVP112929686(MASP2)
VLG121496717(MASP2)
AML100466924(MASP2)
UMR103666866(MASP2)
UAH113270516(MASP2)
UAR123779092(MASP2)
ELK111154710 111154837
LLV125097951
MPUF101678006(MASP2)
ORO101362776(MASP2)
EJU114213204(MASP2)
ZCA113929755(MASP2)
MLX118020013(MASP2)
FCA101099568(MASP2)
PYU121020988(MASP2)
PBG122479914(MASP2)
PPAD109274100(MASP2)
AJU106984902(MASP2)
HHV120233611(MASP2)
BTA505819(MASP2)
BOM102280596(MASP2)
BIU109570390(MASP2)
BBUB102414667(MASP2)
CHX102169785(MASP2)
ODA120880210(MASP2)
CCAD122451961(MASP2)
SSC100302537(MASP2)
CFR102513488(MASP2)
CBAI105070978(MASP2)
CDK105107020(MASP2)
VPC102529062(MASP2)
BACU103005678(MASP2)
LVE103083615(MASP2)
OOR101283337(MASP2)
DLE111187114(MASP2)
PCAD102973281(MASP2)
PSIU116758848(MASP2)
ECB100056770(MASP2)
EPZ103552243(MASP2)
EAI106836680(MASP2)
MYB102255311
MYD102764933(MASP2)
MMYO118675853(MASP2)
MLF102438779(MASP2)
MNA107540993(MASP2)
HAI109381284(MASP2)
DRO112299103(MASP2)
SHON118986356(MASP2)
AJM119064148(MASP2)
PDIC114496855(MASP2)
PHAS123831604(MASP2)
MMF118626289(MASP2)
RFQ117026790(MASP2)
PALE102885750(MASP2)
PGIG120585827(MASP2)
PVP105294376(MASP2)
RAY107513106(MASP2)
MJV108395496(MASP2) 108401888
TOD119235574(MASP2)
SARA101554126(MASP2)
LAV100662293(MASP2)
TMU101342871
DNM101436761(MASP2)
MDO100018957(MASP2)
GAS123240961(MASP2)
SHR100932261(MASP2)
OAA100079601(MASP2)
GGA407089(MASP2)
PCOC116242633(MASP2)
MGP100547191(MASP2)
CJO107323403(MASP2)
NMEL110408613(MASP2)
APLA101801331(MASP2)
ACYG106040226(MASP2)
AFUL116497751(MASP2)
TGU100232734(MASP2)
GFR102032737(MASP2)
PHI102110924(MASP2)
EGZ104133133(MASP2)
PADL103917979(MASP2)
ACHC115342613(MASP2)
AAM106482473(MASP2)
ASN102370755(MASP2)
CPOO109314472(MASP2)
GGN109293678(MASP2)
PSS102453425(MASP2)
CMY102929438(MASP2)
CPIC101950973(MASP2)
TST117868098(MASP2)
CABI116821926(MASP2)
MRV120387680(MASP2)
PVT110073231(MASP2)
SUND121936757(MASP2)
PBI103053568(MASP2)
PMUR107295524(MASP2) 107301108
TSR106548091(MASP2)
PGUT117673072(MASP2)
VKO123030990(MASP2)
PMUA114602450(MASP2)
ZVI118087952(MASP2)
GJA107124364(MASP2)
STOW125445616(MASP2)
XLA108696362(masp2.L) 373654(masp2.S)
XTR496446(masp2)
NPR108789090
RTEM120915654(MASP2)
BBUF120999375(MASP2)
BGAR122928835(MASP2) 122928836
DRE560277(masp2)
SRX107708846
SANH107681988(masp2)
SGH107571531
CCAR109048441
CAUA113041489(masp2) 113076023
PPRM120463137(masp2)
MAMB125260255(masp2)
IPU108268542 108276220(masp2)
PHYP113530393(masp2)
TFD113658081(masp2)
AMEX103023364(masp2)
EEE113581290(masp2)
TRU101078225(masp2)
NCC104954130(masp2)
CGOB115011239(masp2)
ELY117257906(masp2)
PLEP121947425(masp2)
SLUC116041034(masp2)
ECRA117947982(masp2)
PFLV114558539(masp2)
GAT120829410(masp2)
PPUG119223689(masp2)
MSAM119905737(masp2)
CUD121517697(masp2)
MZE101487177(masp2)
ONL100703975(masp2)
OAU116309673(masp2)
OLA101160657(masp2)
OML112159086(masp2)
XMA102226518(masp2)
XCO114141728(masp2) 114142162
XHE116719480(masp2)
PRET103467229(masp2)
PFOR103138924(masp2)
PLAI106949124(masp2)
PMEI106919908(masp2)
GAF122834630(masp2)
CVG107086886
CTUL119797085(masp2)
GMU124861211(masp2)
NFU107379963 107390742(masp2)
KMR108236764(masp2)
ALIM106536928
NWH119417291(masp2)
AOCE111566407(masp2)
CSEM103384955(masp2)
POV109630666(masp2)
SSEN122777836(masp2)
HHIP117764396(masp2)
LCF108876586(masp2)
SDU111219203(masp2)
SLAL111670573(masp2)
XGL120803440(masp2)
HCQ109526567(masp2)
BPEC110164865(masp2)
MALB109968959(masp2)
SASA106572058(masp2) 106597909 106605730
OTW112232616(masp2)
OMY110532320(masp2)
OGO124031969(masp2)
ONE115132195(masp2)
SALP111966480(masp2)
SNH120059317(masp2)
ELS105022975(masp2)
SFM108923279(masp2)
PKI111834328(masp2)
AANG118207316(masp2)
LOC102693055(masp2)
PSPA121328317 121329011(masp2)
ARUT117425426 117432094
LCM102360645(MASP2)
SCLV120328637
PTEP107450699
SDM118204575
LGILOTGIDRAFT_164015
HRJ124252926
 » show all
Reference
1  [PMID:1460414]
  Authors
Matsushita M, Fujita T.
  Title
Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease.
  Journal
J Exp Med 176:1497-502 (1992)
DOI:10.1084/jem.176.6.1497
Reference
2  [PMID:9087411]
  Authors
Thiel S, Vorup-Jensen T, Stover CM, Schwaeble W, Laursen SB, Poulsen K, Willis AC, Eggleton P, Hansen S, Holmskov U, Reid KB, Jensenius JC.
  Title
A second serine protease associated with mannan-binding lectin that activates complement.
  Journal
Nature 386:506-10 (1997)
DOI:10.1038/386506a0
  Sequence
[hsa:10747]
Reference
3  [PMID:11527969]
  Authors
Rossi V, Cseh S, Bally I, Thielens NM, Jensenius JC, Arlaud GJ.
  Title
Substrate specificities of recombinant mannan-binding lectin-associated serine proteases-1 and -2.
  Journal
J Biol Chem 276:40880-7 (2001)
DOI:10.1074/jbc.M105934200
Reference
4  [PMID:12538697]
  Authors
Ambrus G, Gal P, Kojima M, Szilagyi K, Balczer J, Antal J, Graf L, Laich A, Moffatt BE, Schwaeble W, Sim RB, Zavodszky P.
  Title
Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments.
  Journal
J Immunol 170:1374-82 (2003)
DOI:10.4049/jimmunol.170.3.1374
Reference
5  [PMID:15364579]
  Authors
Harmat V, Gal P, Kardos J, Szilagyi K, Ambrus G, Vegh B, Naray-Szabo G, Zavodszky P.
  Title
The structure of MBL-associated serine protease-2 reveals that identical substrate specificities of C1s and MASP-2 are realized through different sets of enzyme-substrate interactions.
  Journal
J Mol Biol 342:1533-46 (2004)
DOI:10.1016/j.jmb.2004.07.014
  Sequence
[hsa:10747]
Reference
6  [PMID:15060079]
  Authors
Chen CB, Wallis R.
  Title
Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases.
  Journal
J Biol Chem 279:26058-65 (2004)
DOI:10.1074/jbc.M401318200
Reference
7  [PMID:15728497]
  Authors
Teillet F, Dublet B, Andrieu JP, Gaboriaud C, Arlaud GJ, Thielens NM.
  Title
The two major oligomeric forms of human mannan-binding lectin: chemical characterization, carbohydrate-binding properties, and interaction with MBL-associated serine proteases.
  Journal
J Immunol 174:2870-7 (2005)
DOI:10.4049/jimmunol.174.5.2870
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.104
IUBMB Enzyme Nomenclature: 3.4.21.104
ExPASy - ENZYME nomenclature database: 3.4.21.104
BRENDA, the Enzyme Database: 3.4.21.104
CAS: 214915-16-9

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