Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity
Comment
From the sarcocarp of the musk melon (Cucumis melo). In peptidase family S8 (subtilisin family). Other endopeptidases from plants, which are less well characterized but presumably of serine-type, include euphorbain from Euphorbia cerifera [6], solanain from horse-nettle Solanum elaeagnifolium [1], hurain from Hura crepitans [2] and tabernamontanain from Tabernamontana grandiflora [3].
History
EC 3.4.21.25 created 1978 (EC 3.4.21.56 created 1972 as EC 3.4.99.7 transferred 1989 to EC 3.4.21.56, deleted 1992, EC 3.4.99.9 created 1972 deleted 1992, EC 3.4.99.21 created 1972 deleted 1992, EC 3.4.99.23 created 1972 deleted 1992, all covered by EC 3.4.21.25)
Reference
1
Authors
Greenberg, D.M. and Winnick, T.
Title
Plant proteases. I. Activation-inhibition reactions.
Journal
J Biol Chem 135:761-773 (1940)
Reference
2
Authors
Jaffe, W.G.
Title
Hurain, a new plant protease from Hura crepitans.
Journal
J Biol Chem 149:1-7 (1943)
Reference
3
Authors
Jaffe, W.G.
Title
A new vegetable proteolytic enzyme of the papain class.