KEGG ENZYME: 3.4.21.63

ENZYME: 3.4.21.63

Entry

EC 3.4.21.63                Enzyme

Name

oryzin;
Aspergillus alkaline proteinase;
aspergillopeptidase B;
API 21;
aspergillopepsin B;
aspergillopepsin F;
Aspergillus candidus alkaline proteinase;
Aspergillus flavus alkaline proteinase;
Aspergillus melleus semi-alkaline proteinase;
Aspergillus oryzae alkaline proteinase;
Aspergillus parasiticus alkaline proteinase;
Aspergillus serine proteinase;
Aspergillus sydowi alkaline proteinase;
Aspergillus soya alkaline proteinase;
Aspergillus melleus alkaline proteinase;
Aspergillus sulphureus alkaline proteinase;
prozyme;
P 5380;
kyorinase;
seaprose S;
semi-alkaline protease;
sumizyme MP;
prozyme 10;
onoprose;
onoprose SA;
protease P;
promelase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides

Comment

A peptidase of family S8 (subtilisin family), not containing cysteine, that is the predominant extracellular alkaline endopeptidase of the mold Aspergillus oryzae. Identical or closely related enzymes are produced by A. flavus and A. sojae [2,3,4]

History

EC 3.4.21.63 created 1992 (EC 3.4.21.14 created 1961 as EC 3.4.4.16, transferred 1972 to EC 3.4.21.14, modified 1986, part incorporated 1992)

Orthology

K18549

oryzin

Genes

LPIC:

129256198 129259979 129262850 129265598 129272300 129272784 129272867 129272868 129272891 129272903 129272908 129272909 129272980 129273147 129273275 129273423 129273425 129273461 129273603 129277727 129277728 129282672

AJJ:	139967278 139967279 139967533 139968164 139982356

ARUN:

117287995 117291474 117294877 117295046 117296317 117298904 117302960 117306589 117307157

AAMU:

139933889 139935299 139937724 139939621 139939786 139939976 139940584 139940744 139940976 139954323 139954324

AFIL:

140135574 140142407 140147731 140151679 140151837 140151839 140152092 140152870 140154363 140155954 140161788 140161789 140163254 140163281 140166778 140168719 140171440 140172760

PFLL:

139121574 139123286 139128029 139133232 139133237 139137496 139137499 139137500 139137501 139151459

PBEL:

QC761_0074380

PPSD:

QC762_0074970 QC762_510310(SUB2_3)

PPSP:

QC763_0080550 QC763_510310(SUB2_3)

PPSA:

QC764_0074120 QC764_510310(SUB2_3)

PGRI:

PgNI_00992 PgNI_09125 PgNI_10512

FPOA:

FPOAC1_000849 FPOAC1_003675 FPOAC1_004570 FPOAC1_005648 FPOAC1_005685(PRB1_1) FPOAC1_007674(PRB1_2) FPOAC1_010003

FVN:	FVRRES_01015 FVRRES_04017 FVRRES_05068 FVRRES_06221 FVRRES_06258 FVRRES_08152 FVRRES_10849

FFC:	NCS54_00160300 NCS54_00189200 NCS54_00381600 NCS54_00958500 NCS54_01329600

FKR:	NCS57_00157500 NCS57_00187400 NCS57_00387000 NCS57_00979600 NCS57_01316600

FMU:	J7337_001591 J7337_001923(PRB1_1) J7337_006142 J7337_006306 J7337_007904 J7337_008478 J7337_010880 J7337_012173 J7337_012662(PRB1_2)

TATV:

25777411(TrAtP1_003115) 25782294(TrAtP1_011940)

TASP:

36609006(TrAFT101_006824) 36610185(PRB1)

MBRN:

26240007(ALP_1) 26242542(ALP_2) 26243488(alp1_6) 26243761(prb1) 26246022(alp1_2) 26246023(alp1_3) 26246901(alp1_7) 26247590(alp1_5) 26247814(alp1_1)

PCHM:

VFPPC_01236 VFPPC_05714 VFPPC_06535 VFPPC_06603

AMUS:

LMH87_006112 LMH87_010316

PTKZ:

JDV02_003813 JDV02_004629 JDV02_006290 JDV02_006357 JDV02_010728(ALP1_2)

CDET:

87939591(CDEST_03088) 87940465(CDEST_03962) 87943881(CDEST_07378) 87945197(CDEST_08694) 87948556(CDEST_12056) 87951296(CDEST_14796)

ANI:	ANIA_05558 ANIA_10030

AFM:	AFUA_4G11800 AFUA_5G09210

ACT:	ACLA_011360 ACLA_050840

NFI:	NFIA_078120 NFIA_104430

AOR:	AO090003001036 AO090020000517

ANG:	An07g03880(pepC) An09g03780(pepD)

AFV:	AFLA_000633 AFLA_010140

ALUC:

AKAW2_11802A(SUB8) AKAW2_40417S(ALP1)

ACHE:

ACHE_10233S(ALP1) ACHE_50640S(SUB8)

APUU:

APUU_11890S(SUB8) APUU_40913S(ALP1)

POU:	POX_a00593

TMF:	EYB26_003982

TRG:	TRUGW13939_10665

PTRR:

6346972(PtrM4_145250)

ADAC:

96081870(ACET3X_001548) 96088216(ACET3X_007894)

ARAB:

EKO05_0002227 EKO05_0010779

CBET:

CB0940_02328 CB0940_04215

RSX:	RhiXN_00065 RhiXN_05399 RhiXN_07378 RhiXN_09136

RIE:	OCT59_005554 OCT59_022140 OCT59_022323 OCT59_022326 OCT59_022331 OCT59_029871 OCT59_030016

» show all

Reference

Authors

Nakagawa Y.

Title

Alkaline proteinases from Aspergillus.

Journal

Methods Enzymol 19:581-591 (1970)

Reference

Authors

Hayashi K, Terada M.

Title

Some characteristics of hydrolysis of synthetic substrates and proteins by the alkaline proteases from Aspergillus sojae.

Journal

Agric Biol Chem 36:1755-1765 (1972)

Reference

3 [PMID:4623338]

Authors

Turkova J, Mikes O, Hayashi K, Danno G, Polgar L.

Title

Alkaline proteinases of the genus Aspergillus.

Journal

Biochim Biophys Acta 257:257-63 (1972)
DOI:10.1016/0005-2795(72)90277-2

Reference

4 [PMID:4441086]

Authors

Morihara K, Oka T, Tsuzuki H.

Title

Comparative study of various serine alkaline proteinases from microorganisms. Esterase activity against N-acylated peptide ester substrates.

Journal

Arch Biochem Biophys 165:72-9 (1974)
DOI:10.1016/0003-9861(74)90143-X

Reference

5 [PMID:4859351]

Authors

Spadari S, Subramanian AR, Kalnitsky G.

Title

Highly restricted specificity of the serine proteinase aspergillopeptidase B.

Journal

Biochim Biophys Acta 359:267-72 (1974)
DOI:10.1016/0005-2795(74)90224-4

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.63

IUBMB Enzyme Nomenclature:

3.4.21.63

ExPASy - ENZYME nomenclature database:

3.4.21.63

BRENDA, the Enzyme Database:

3.4.21.63

CAS:

9074-07-1

All links

Protein sequence (266)   
   UniProt (226)   
   SWISS-PROT (10)   
   RefSeq(pep) (20)   
   PDBSTR (10)   
DNA sequence (72)   
   RefSeq(nuc) (37)   
   GenBank (19)   
   EMBL (16)   
3D Structure (4)   
   PDB (4)   
Protein domain (11)   
   InterPro (10)   
   PROSITE(DOC) (1)   
All databases (353)   

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