Entry
Name
oryzin;
Aspergillus alkaline proteinase;
aspergillopeptidase B;
API 21;
aspergillopepsin B;
aspergillopepsin F;
Aspergillus candidus alkaline proteinase;
Aspergillus flavus alkaline proteinase;
Aspergillus melleus semi-alkaline proteinase;
Aspergillus oryzae alkaline proteinase;
Aspergillus parasiticus alkaline proteinase;
Aspergillus serine proteinase;
Aspergillus sydowi alkaline proteinase;
Aspergillus soya alkaline proteinase;
Aspergillus melleus alkaline proteinase;
Aspergillus sulphureus alkaline proteinase;
prozyme;
P 5380;
kyorinase;
seaprose S;
semi-alkaline protease;
sumizyme MP;
prozyme 10;
onoprose;
onoprose SA;
protease P;
promelase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides
Comment
A peptidase of family S8 (subtilisin family), not containing cysteine, that is the predominant extracellular alkaline endopeptidase of the mold Aspergillus oryzae. Identical or closely related enzymes are produced by A. flavus and A. sojae [2,3,4]
History
EC 3.4.21.63 created 1992 (EC 3.4.21.14 created 1961 as EC 3.4.4.16, transferred 1972 to EC 3.4.21.14, modified 1986, part incorporated 1992)
Orthology
Genes
AFM : AFUA_4G11800 AFUA_5G09210
ACT : ACLA_011360 ACLA_050840
NFI : NFIA_078120 NFIA_104430
AOR : AO090003001036 AO090020000517
ANG : ANI_1_1348084(An09g03780) ANI_1_476064(An07g03880)
AFV : AFLA_000633 AFLA_010140
ALUC : AKAW2_11802A(SUB8) AKAW2_40417S(ALP1)
ACHE : ACHE_10233S(ALP1) ACHE_50640S(SUB8)
APUU : APUU_11890S(SUB8) APUU_40913S(ALP1)
Taxonomy
Reference
1
Authors
Nakagawa, Y.
Title
Alkaline proteinases from Aspergillus.
Journal
Methods Enzymol 19:581-591 (1970)
Reference
2
Authors
Hayashi, K. and Terada, M.
Title
Some characteristics of hydrolysis of synthetic substrates and proteins by the alkaline proteases from Aspergillus sojae.
Journal
Agric Biol Chem 36:1755-1765 (1972)
Reference
Authors
Turkova J, Mikes O, Hayashi K, Danno G, Polgar L.
Title
Alkaline proteinases of the genus Aspergillus.
Journal
Reference
Authors
Morihara K, Oka T, Tsuzuki H.
Title
Comparative study of various serine alkaline proteinases from microorganisms. Esterase activity against N-acylated peptide ester substrates.
Journal
Reference
Authors
Spadari S, Subramanian AR, Kalnitsky G.
Title
Highly restricted specificity of the serine proteinase aspergillopeptidase B.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 3.4.21.63